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The map label for this gene is gatB

Identifier: 194334877

GI number: 194334877

Start: 2263123

End: 2264568

Strand: Reverse

Name: gatB

Synonym: Paes_2080

Alternate gene names: 194334877

Gene position: 2264568-2263123 (Counterclockwise)

Preceding gene: 194334878

Following gene: 194334875

Centisome position: 90.12

GC content: 50.55

Gene sequence:

>1446_bases
ATGAGCTATGAATTAGTTGTGGGCCTTGAAGTCCACTGTCAGTTAAATACGAACAGCAAAGCGTTCTGCGGCTGTTCGAC
CGAGTTCGGCAAACCGGCAAACACCAATGTTTGTCCGGTTTGTCTGGCTTTACCGGGGGCCCTGCCGGTCCTGAACCAGA
GAGTTGTCGACGATGCCATCAAGCTTGGGATGTCAACAGGTTGTTCCATCGCGCCTCATTCTGTTCTTGCCAGGAAAAAC
TATTTTTATCCCGATCTGCCGAAAGGATATCAGATTTCACAGTTTGAAGAACCTATCTGTCTTGAGGGTTGTCTATCGAT
CGATGCGGGTGAGGGGCAGAGGGATATACGTCTGATTCGAATACACATCGAAGAGGATGCCGGTAAATCTATTCACGATA
TCGGTGACGATACCTTTATCGATGCCAACCGGAGTGGCGTTCCTCTTCTTGAAATAGTCAGTTACCCCGATATCCGTTCT
TCGAAAGAGGCTTCGGCATATCTGCAGAAGCTGCGTCAGATTGTCAAATATCTCGGTATTTCCGATGGGAATATGGAGGA
GGGCAGTCTTCGTTGTGATGCCAACGTGTCGATGCGCCCGAGAGGCGAGAGCGAATACGGCACGAGAACCGAGATCAAGA
ATATGAACTCGTTCAAGAGCGTTGAAAAGGCGATCGAGTATGAAGCGCAGCGTCATATTGACGTGCTTGAAGCCGGAGGA
ACCATTGTTCAGGAAACCCGTCTCTGGGATGCCGACAAGCTTGAAACCCGTTCCATGCGCGGCAAGGAGTTCTCTCATGA
CTACCGGTATTTTCCGGACCCTGATCTGGTTCCGATCGCTGTTGACGATGCAATGCTTGATCGTCTCCGAAAAGAACTTC
CCGAGTTTCCCGAGGCCCGTGCTGCCCGTTTTGTCGAGGAGTACGATATTCCGCCATATGATGCAGGCGTCTTGACTGCG
GAACGTGAAATCGCCGATTACTTCGAGGAGACGGTGAGGGTTTCCGGTGATGGAAAAGCTGCTTCAAACTGGGTCATGGG
CGAGGTGCTCCGGACCCTTAAAGAGAAGTATATTCCGATTGCCTCCTTTACTATTTCGCCTGAGCGGCTCGGTGAACTGA
TAGGCCTCATCGCGAAGGGAACGATCAGTAACACCATCGCCAAGCAGGTTTTCGAGAAAATGCAGGAGCTTGACGAGGGG
CCGCAGGAAATTGTCAGGAAAGAAGGCCTTGCTCAGGTTTCAGATACCGGTGCGATCGAGGGTGTCGTTCAGGAGATTAT
TGATGCTAACCCGGCACAGGTTGAACAGTACAGGAGCGGTAAAACCAGGATTTTCGGCTTTTTTGTCGGACAGTGTATGA
AAGAGATGAAAGGCAAGGCCAATCCTGCGGTGGTCAATGATATTCTGAAACAGAAACTTGATGAGGGTATGACGGGCGAT
GCCTAA

Upstream 100 bases:

>100_bases
AAGTTTTCGTTTCAGCCCTATTGAGCGGTTTTCAGGGTTTACCAGGCTTTTTTTGTGTGAACCGTCAACAGGTGTTTATC
AATATTGTTCTTGAAATTCA

Downstream 100 bases:

>100_bases
CCGGGATCGCTGAACAGGTCGGTATAGCGCGGGGTTTCGGATGGCTTGCCTGATTGAGATTCCGGGTTGATCGTTTCAAG
TTTCTGTCTGGAATCCTGCA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSYELVVGLEVHCQLNTNSKAFCGCSTEFGKPANTNVCPVCLALPGALPVLNQRVVDDAIKLGMSTGCSIAPHSVLARKN
YFYPDLPKGYQISQFEEPICLEGCLSIDAGEGQRDIRLIRIHIEEDAGKSIHDIGDDTFIDANRSGVPLLEIVSYPDIRS
SKEASAYLQKLRQIVKYLGISDGNMEEGSLRCDANVSMRPRGESEYGTRTEIKNMNSFKSVEKAIEYEAQRHIDVLEAGG
TIVQETRLWDADKLETRSMRGKEFSHDYRYFPDPDLVPIAVDDAMLDRLRKELPEFPEARAARFVEEYDIPPYDAGVLTA
EREIADYFEETVRVSGDGKAASNWVMGEVLRTLKEKYIPIASFTISPERLGELIGLIAKGTISNTIAKQVFEKMQELDEG
PQEIVRKEGLAQVSDTGAIEGVVQEIIDANPAQVEQYRSGKTRIFGFFVGQCMKEMKGKANPAVVNDILKQKLDEGMTGD
A

Sequences:

>Translated_481_residues
MSYELVVGLEVHCQLNTNSKAFCGCSTEFGKPANTNVCPVCLALPGALPVLNQRVVDDAIKLGMSTGCSIAPHSVLARKN
YFYPDLPKGYQISQFEEPICLEGCLSIDAGEGQRDIRLIRIHIEEDAGKSIHDIGDDTFIDANRSGVPLLEIVSYPDIRS
SKEASAYLQKLRQIVKYLGISDGNMEEGSLRCDANVSMRPRGESEYGTRTEIKNMNSFKSVEKAIEYEAQRHIDVLEAGG
TIVQETRLWDADKLETRSMRGKEFSHDYRYFPDPDLVPIAVDDAMLDRLRKELPEFPEARAARFVEEYDIPPYDAGVLTA
EREIADYFEETVRVSGDGKAASNWVMGEVLRTLKEKYIPIASFTISPERLGELIGLIAKGTISNTIAKQVFEKMQELDEG
PQEIVRKEGLAQVSDTGAIEGVVQEIIDANPAQVEQYRSGKTRIFGFFVGQCMKEMKGKANPAVVNDILKQKLDEGMTGD
A
>Mature_480_residues
SYELVVGLEVHCQLNTNSKAFCGCSTEFGKPANTNVCPVCLALPGALPVLNQRVVDDAIKLGMSTGCSIAPHSVLARKNY
FYPDLPKGYQISQFEEPICLEGCLSIDAGEGQRDIRLIRIHIEEDAGKSIHDIGDDTFIDANRSGVPLLEIVSYPDIRSS
KEASAYLQKLRQIVKYLGISDGNMEEGSLRCDANVSMRPRGESEYGTRTEIKNMNSFKSVEKAIEYEAQRHIDVLEAGGT
IVQETRLWDADKLETRSMRGKEFSHDYRYFPDPDLVPIAVDDAMLDRLRKELPEFPEARAARFVEEYDIPPYDAGVLTAE
REIADYFEETVRVSGDGKAASNWVMGEVLRTLKEKYIPIASFTISPERLGELIGLIAKGTISNTIAKQVFEKMQELDEGP
QEIVRKEGLAQVSDTGAIEGVVQEIIDANPAQVEQYRSGKTRIFGFFVGQCMKEMKGKANPAVVNDILKQKLDEGMTGDA

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=36.7676767676768, Blast_Score=320, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI115534094, Length=299, Percent_Identity=37.4581939799331, Blast_Score=197, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6319391, Length=509, Percent_Identity=30.4518664047151, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI45550808, Length=482, Percent_Identity=33.195020746888, Blast_Score=278, Evalue=6e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_PROA2 (B4S5P3)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002016737.1
- ProteinModelPortal:   B4S5P3
- SMR:   B4S5P3
- GeneID:   6460664
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_2080
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53398; Mature: 53267

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYELVVGLEVHCQLNTNSKAFCGCSTEFGKPANTNVCPVCLALPGALPVLNQRVVDDAI
CCCEEEEEEEEEEEECCCCCEEECCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHH
KLGMSTGCSIAPHSVLARKNYFYPDLPKGYQISQFEEPICLEGCLSIDAGEGQRDIRLIR
HHCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEEEE
IHIEEDAGKSIHDIGDDTFIDANRSGVPLLEIVSYPDIRSSKEASAYLQKLRQIVKYLGI
EEEECCCCCHHHHCCCCCEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
SDGNMEEGSLRCDANVSMRPRGESEYGTRTEIKNMNSFKSVEKAIEYEAQRHIDVLEAGG
CCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
TIVQETRLWDADKLETRSMRGKEFSHDYRYFPDPDLVPIAVDDAMLDRLRKELPEFPEAR
CEEEHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHCCCCCHHH
AARFVEEYDIPPYDAGVLTAEREIADYFEETVRVSGDGKAASNWVMGEVLRTLKEKYIPI
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHCCCE
ASFTISPERLGELIGLIAKGTISNTIAKQVFEKMQELDEGPQEIVRKEGLAQVSDTGAIE
EEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHCCHHHH
GVVQEIIDANPAQVEQYRSGKTRIFGFFVGQCMKEMKGKANPAVVNDILKQKLDEGMTGD
HHHHHHHCCCHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
A
C
>Mature Secondary Structure 
SYELVVGLEVHCQLNTNSKAFCGCSTEFGKPANTNVCPVCLALPGALPVLNQRVVDDAI
CCEEEEEEEEEEEECCCCCEEECCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHH
KLGMSTGCSIAPHSVLARKNYFYPDLPKGYQISQFEEPICLEGCLSIDAGEGQRDIRLIR
HHCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEEEE
IHIEEDAGKSIHDIGDDTFIDANRSGVPLLEIVSYPDIRSSKEASAYLQKLRQIVKYLGI
EEEECCCCCHHHHCCCCCEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
SDGNMEEGSLRCDANVSMRPRGESEYGTRTEIKNMNSFKSVEKAIEYEAQRHIDVLEAGG
CCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
TIVQETRLWDADKLETRSMRGKEFSHDYRYFPDPDLVPIAVDDAMLDRLRKELPEFPEAR
CEEEHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHCCCCCHHH
AARFVEEYDIPPYDAGVLTAEREIADYFEETVRVSGDGKAASNWVMGEVLRTLKEKYIPI
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHCCCE
ASFTISPERLGELIGLIAKGTISNTIAKQVFEKMQELDEGPQEIVRKEGLAQVSDTGAIE
EEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHCCHHHH
GVVQEIIDANPAQVEQYRSGKTRIFGFFVGQCMKEMKGKANPAVVNDILKQKLDEGMTGD
HHHHHHHCCCHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA