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The map label for this gene is dacB [H]

Identifier: 194334807

GI number: 194334807

Start: 2207146

End: 2208099

Strand: Reverse

Name: dacB [H]

Synonym: Paes_2010

Alternate gene names: 194334807

Gene position: 2208099-2207146 (Counterclockwise)

Preceding gene: 194334808

Following gene: 194334802

Centisome position: 87.87

GC content: 50.31

Gene sequence:

>954_bases
ATGATGAAGAGTCCGCTCCTGACGGGCCGCCGATCAGGTGCTGTTTGCACGATGACGGCAGTTTTTGCTTGTCTCATGTT
TTTTTGTTTCCCTGTGCCGTCGATAGCCGAGCCCCACGCAAAGGATTCTCTTAAGGTGGAGCCTCTCGATATCGCATCAT
ATATTCTCAAGGATATAGGCCGTTCAACGGTGCTGCTTGAAAAAGACCCCGGGACACGTCATGAACCGGCAAGTCTCACG
AAGATACTTACCTGCATTATTGCTATTGAGAGCGCCAGGCTCAATGAGTTTGTGACGATTCCTCTTGAAGCGACACAGGT
CGAACCGACCAAGGCCGGTTTTCTGCCGGGCGAAAAAATACGACTTCTTGATCTGGTTAAAGCCGCAATGGTCAAATCGA
GCAATGATGCAGCTTTTGCCATCGCAATACACCTTGGCGGAAGTGTTCAGGGGTTTGCGGCCATGATGAACGCCAAAGCC
GCTGAGCTTGGGCTTTCCCATTCCTGTTTTACCAATCCGGCCGGTTTCGATCGCCATCAGTACGTTGGCCATTATTCAAC
TGCTGGAGATATGCTGCGTTTGACCGAGTATGCGATCGGCAATCAGATTTTCAACTATATAGCAGGTCTCGACGAGGTCT
CGATCACTGAAAAACGTTCCGGCAAAATCTACAGGCTCAAGACGAGCAATAAATTGCTTCATCGGTATCCTTATGCTGTC
GGAATCAAAACCGGTTATACCTTCAGGGCAGGCAAGTGTCTTATTGCAAGGGCAAAAAAAGGTGACCGTGATGTCGTACT
GGTGATGCTCAATGCGCGTCTTGATCGTTGGTCGGTGGCAGAAGAACTTTTCGAGAAGGCCTTTTCGCTTCCGGGTTCTG
AGCGGGCTCCGTTCGGGCAGCGCAGTCACGATATGCGAGCATCACTTATTCTGAAGGGCAATGAGCTGCAGTAG

Upstream 100 bases:

>100_bases
GCTACTCGTTTCGATGCCATGCTGTGCTGAATACGGGTGTTATTTTGTTACCTTGGAAGTGATGAAGAGGTTTGTTGATA
TTGTTTGATAAGGAGAAGAG

Downstream 100 bases:

>100_bases
AAGAAAAAATAAACCCCGGTCAACTGCCGGGGTTTTCATAGATACTGTCGGGAACTGCATCAGTTATTGACGCGCACAGC
CTCTTCTGTTGAAAAGAAAA

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MMKSPLLTGRRSGAVCTMTAVFACLMFFCFPVPSIAEPHAKDSLKVEPLDIASYILKDIGRSTVLLEKDPGTRHEPASLT
KILTCIIAIESARLNEFVTIPLEATQVEPTKAGFLPGEKIRLLDLVKAAMVKSSNDAAFAIAIHLGGSVQGFAAMMNAKA
AELGLSHSCFTNPAGFDRHQYVGHYSTAGDMLRLTEYAIGNQIFNYIAGLDEVSITEKRSGKIYRLKTSNKLLHRYPYAV
GIKTGYTFRAGKCLIARAKKGDRDVVLVMLNARLDRWSVAEELFEKAFSLPGSERAPFGQRSHDMRASLILKGNELQ

Sequences:

>Translated_317_residues
MMKSPLLTGRRSGAVCTMTAVFACLMFFCFPVPSIAEPHAKDSLKVEPLDIASYILKDIGRSTVLLEKDPGTRHEPASLT
KILTCIIAIESARLNEFVTIPLEATQVEPTKAGFLPGEKIRLLDLVKAAMVKSSNDAAFAIAIHLGGSVQGFAAMMNAKA
AELGLSHSCFTNPAGFDRHQYVGHYSTAGDMLRLTEYAIGNQIFNYIAGLDEVSITEKRSGKIYRLKTSNKLLHRYPYAV
GIKTGYTFRAGKCLIARAKKGDRDVVLVMLNARLDRWSVAEELFEKAFSLPGSERAPFGQRSHDMRASLILKGNELQ
>Mature_317_residues
MMKSPLLTGRRSGAVCTMTAVFACLMFFCFPVPSIAEPHAKDSLKVEPLDIASYILKDIGRSTVLLEKDPGTRHEPASLT
KILTCIIAIESARLNEFVTIPLEATQVEPTKAGFLPGEKIRLLDLVKAAMVKSSNDAAFAIAIHLGGSVQGFAAMMNAKA
AELGLSHSCFTNPAGFDRHQYVGHYSTAGDMLRLTEYAIGNQIFNYIAGLDEVSITEKRSGKIYRLKTSNKLLHRYPYAV
GIKTGYTFRAGKCLIARAKKGDRDVVLVMLNARLDRWSVAEELFEKAFSLPGSERAPFGQRSHDMRASLILKGNELQ

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=251, Percent_Identity=31.0756972111554, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786851, Length=336, Percent_Identity=28.2738095238095, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87082022, Length=236, Percent_Identity=30.5084745762712, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI87082054, Length=233, Percent_Identity=27.8969957081545, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 34774; Mature: 34774

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKSPLLTGRRSGAVCTMTAVFACLMFFCFPVPSIAEPHAKDSLKVEPLDIASYILKDIG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECHHHHHHHHHHHCC
RSTVLLEKDPGTRHEPASLTKILTCIIAIESARLNEFVTIPLEATQVEPTKAGFLPGEKI
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCHH
RLLDLVKAAMVKSSNDAAFAIAIHLGGSVQGFAAMMNAKAAELGLSHSCFTNPAGFDRHQ
HHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCHH
YVGHYSTAGDMLRLTEYAIGNQIFNYIAGLDEVSITEKRSGKIYRLKTSNKLLHRYPYAV
HCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEECCCHHHHHCCEEE
GIKTGYTFRAGKCLIARAKKGDRDVVLVMLNARLDRWSVAEELFEKAFSLPGSERAPFGQ
EECCCCEEECCCEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
RSHDMRASLILKGNELQ
CCCCCEEEEEEECCCCC
>Mature Secondary Structure
MMKSPLLTGRRSGAVCTMTAVFACLMFFCFPVPSIAEPHAKDSLKVEPLDIASYILKDIG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECHHHHHHHHHHHCC
RSTVLLEKDPGTRHEPASLTKILTCIIAIESARLNEFVTIPLEATQVEPTKAGFLPGEKI
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCHH
RLLDLVKAAMVKSSNDAAFAIAIHLGGSVQGFAAMMNAKAAELGLSHSCFTNPAGFDRHQ
HHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCHH
YVGHYSTAGDMLRLTEYAIGNQIFNYIAGLDEVSITEKRSGKIYRLKTSNKLLHRYPYAV
HCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEECCCHHHHHCCEEE
GIKTGYTFRAGKCLIARAKKGDRDVVLVMLNARLDRWSVAEELFEKAFSLPGSERAPFGQ
EECCCCEEECCCEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
RSHDMRASLILKGNELQ
CCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]