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The map label for this gene is mscL

Identifier: 194334431

GI number: 194334431

Start: 1770089

End: 1770493

Strand: Reverse

Name: mscL

Synonym: Paes_1626

Alternate gene names: 194334431

Gene position: 1770493-1770089 (Counterclockwise)

Preceding gene: 194334444

Following gene: 194334426

Centisome position: 70.46

GC content: 47.41

Gene sequence:

>405_bases
ATGGGATTTATTCAGGAGTTTAAGGACTTCGCGATGCGGGGAAATGTTGTTGATCTGGCTGTTGGTATCATTATCGGCGG
AGCATTCGGAAAGGTTGTGACGGCACTTGTGGATGGCGTTCTTATGCCTCCGATCGGTTTATTGATAGGGGGGGTGAATT
TTGATCAGCTTGCGTTCGAATTGAGGGCTGCCACAGCAGAGAGTGCGGCGGTGAGTCTCAACTACGGTGCATTTTTGCAA
ACGATCGTTGATTTTGTCATCATAGCCTTTTCAATATTTGTTGTTATCAAGGCGTTGAATTCACTGAAACGAAAGAGTGA
GGAAGCTCCCAAAGCTCCTCCGGTACCGTCGAAAGAAGAGGTGTTGCTCGGTGAGATTCGCGATCTGTTGAAAGAGAGAG
GTTAG

Upstream 100 bases:

>100_bases
TTTGCCTATATTTTTACAAGGCGGCATCGGTGCTTTTTTTCTTGATGCCTGCTTTTGAGTGTGGTAGGGTTTTTTCCAAC
GTCTCATGGAGGAGAGGATT

Downstream 100 bases:

>100_bases
GGGTGTGTTTTTGCTGCTGGAGCGGTTAAGCTGTCGATGCATGCCGCTCCATGCTTTCAGGGTCGGTATTTACGGAAATC
AATAGAGAATTTTTTGACTC

Product: large conductance mechanosensitive channel protein

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 134; Mature: 133

Protein sequence:

>134_residues
MGFIQEFKDFAMRGNVVDLAVGIIIGGAFGKVVTALVDGVLMPPIGLLIGGVNFDQLAFELRAATAESAAVSLNYGAFLQ
TIVDFVIIAFSIFVVIKALNSLKRKSEEAPKAPPVPSKEEVLLGEIRDLLKERG

Sequences:

>Translated_134_residues
MGFIQEFKDFAMRGNVVDLAVGIIIGGAFGKVVTALVDGVLMPPIGLLIGGVNFDQLAFELRAATAESAAVSLNYGAFLQ
TIVDFVIIAFSIFVVIKALNSLKRKSEEAPKAPPVPSKEEVLLGEIRDLLKERG
>Mature_133_residues
GFIQEFKDFAMRGNVVDLAVGIIIGGAFGKVVTALVDGVLMPPIGLLIGGVNFDQLAFELRAATAESAAVSLNYGAFLQT
IVDFVIIAFSIFVVIKALNSLKRKSEEAPKAPPVPSKEEVLLGEIRDLLKERG

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=134, Percent_Identity=60.4477611940298, Blast_Score=167, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_PROA2 (B4S9H4)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002016291.1
- ProteinModelPortal:   B4S9H4
- SMR:   B4S9H4
- GeneID:   6459872
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_1626
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- ProtClustDB:   CLSK2339382
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 14305; Mature: 14174

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1c922c74)-; HASH(0x1e6f1af8)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFIQEFKDFAMRGNVVDLAVGIIIGGAFGKVVTALVDGVLMPPIGLLIGGVNFDQLAFE
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHCCCCHHHHHHH
LRAATAESAAVSLNYGAFLQTIVDFVIIAFSIFVVIKALNSLKRKSEEAPKAPPVPSKEE
HHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
VLLGEIRDLLKERG
HHHHHHHHHHHHCH
>Mature Secondary Structure 
GFIQEFKDFAMRGNVVDLAVGIIIGGAFGKVVTALVDGVLMPPIGLLIGGVNFDQLAFE
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHCCCCHHHHHHH
LRAATAESAAVSLNYGAFLQTIVDFVIIAFSIFVVIKALNSLKRKSEEAPKAPPVPSKEE
HHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
VLLGEIRDLLKERG
HHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA