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The map label for this gene is ispE

Identifier: 194334396

GI number: 194334396

Start: 1733168

End: 1734028

Strand: Reverse

Name: ispE

Synonym: Paes_1591

Alternate gene names: 194334396

Gene position: 1734028-1733168 (Counterclockwise)

Preceding gene: 194334399

Following gene: 194334393

Centisome position: 69.0

GC content: 53.54

Gene sequence:

>861_bases
ATGCAGAGTATCAGCGTCAAGGCATATGCTAAAATCAATCTTGGCCTCCTGATTACCGGCAAACGGCCGGATGGCTACCA
TACTCTCGAGACCGTGTTTGCCCCGATCAACTGGTATGACGAGCTGACATTTTCTCCTTCGCAGGAGGTGCGGATGAGCT
GTACCAATCAGGATCTTCCCTGTGACGACAGCAATCTCTGTATCAGGGCTGCAAAGGCGTTGCGTGATTTCTCCGGTGTT
TCATCGGGCGTGGAGATAGGGCTCGCCAAACACATTCCTTTCGGTGCCGGTCTTGGCGGCGGTAGCAGTGATGCTGCCTC
GACGCTCAGAGTGCTCAACAGAATGTGGAATATCAACGCATCATCGCATGATCTGCACCGTATCGCCGTCGGTCTTGGTG
CCGATGTTCCCTATTTTCTGGAGTCCGGCGGACTGGCCTATGCCGCCGGGATTGGAGAGGAGCTCGAGGACCTTGAACTG
ACCCTCCCGTTTTCTATTGTCACGCTTTTTCCCGGCGATCATATTTCCACGGTATGGGCGTACCGCAATTTTTATGCCTG
TTTCGAGAGGGAGATTCCGGATTTGAAAGGACTGGTGCGGTCTTTGTGCCAGGCCAGGGATACTTCCGTTCTGGAGGTGT
TCGACAACGATTTTGAACCGGTGGTTTTCGACCATTATCCGGCCGTACGAGAGGCAAAAGACGCGCTTGTGCAGTCAGGG
AGTTTTTTTGCGTCTCTTTCCGGAAGCGGTTCGGCTGTTTTTGGACTTTTTGAAAACGACAGCGATGCAGAGCGAGCTGC
AGAGCAGTTGTCGGCAACCTACCGGGCAAGATTCACTCCGGCATCCTTTGCTATGGCCTGA

Upstream 100 bases:

>100_bases
ATATCCGGAATACATGCATGAGTATTGTGCGCGGTTCAGGTTAGTGAAGGACCGTTTCCACAAAATATGGTAAATATTCA
TTTAAAATCAATCAGTTTCC

Downstream 100 bases:

>100_bases
GATGATCTTCTTTCAATCATGGTATGGAGAGCCGTTGTTTTACCCCTGCGGTCTCCATACCATCACATCGTTGAGTTCAG
AGGCAAAGAGTCTGAGATCT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MQSISVKAYAKINLGLLITGKRPDGYHTLETVFAPINWYDELTFSPSQEVRMSCTNQDLPCDDSNLCIRAAKALRDFSGV
SSGVEIGLAKHIPFGAGLGGGSSDAASTLRVLNRMWNINASSHDLHRIAVGLGADVPYFLESGGLAYAAGIGEELEDLEL
TLPFSIVTLFPGDHISTVWAYRNFYACFEREIPDLKGLVRSLCQARDTSVLEVFDNDFEPVVFDHYPAVREAKDALVQSG
SFFASLSGSGSAVFGLFENDSDAERAAEQLSATYRARFTPASFAMA

Sequences:

>Translated_286_residues
MQSISVKAYAKINLGLLITGKRPDGYHTLETVFAPINWYDELTFSPSQEVRMSCTNQDLPCDDSNLCIRAAKALRDFSGV
SSGVEIGLAKHIPFGAGLGGGSSDAASTLRVLNRMWNINASSHDLHRIAVGLGADVPYFLESGGLAYAAGIGEELEDLEL
TLPFSIVTLFPGDHISTVWAYRNFYACFEREIPDLKGLVRSLCQARDTSVLEVFDNDFEPVVFDHYPAVREAKDALVQSG
SFFASLSGSGSAVFGLFENDSDAERAAEQLSATYRARFTPASFAMA
>Mature_286_residues
MQSISVKAYAKINLGLLITGKRPDGYHTLETVFAPINWYDELTFSPSQEVRMSCTNQDLPCDDSNLCIRAAKALRDFSGV
SSGVEIGLAKHIPFGAGLGGGSSDAASTLRVLNRMWNINASSHDLHRIAVGLGADVPYFLESGGLAYAAGIGEELEDLEL
TLPFSIVTLFPGDHISTVWAYRNFYACFEREIPDLKGLVRSLCQARDTSVLEVFDNDFEPVVFDHYPAVREAKDALVQSG
SFFASLSGSGSAVFGLFENDSDAERAAEQLSATYRARFTPASFAMA

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=268, Percent_Identity=32.4626865671642, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_PROA2 (B4S9D9)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002016256.1
- ProteinModelPortal:   B4S9D9
- SMR:   B4S9D9
- GeneID:   6460072
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_1591
- HOGENOM:   HBG734593
- OMA:   PAVMVNP
- ProtClustDB:   PRK14616
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31030; Mature: 31030

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSISVKAYAKINLGLLITGKRPDGYHTLETVFAPINWYDELTFSPSQEVRMSCTNQDLP
CCCCEEEEEEEEEEEEEEECCCCCCCHHHHHHHHCCCCHHCCCCCCCHHHHEEECCCCCC
CDDSNLCIRAAKALRDFSGVSSGVEIGLAKHIPFGAGLGGGSSDAASTLRVLNRMWNINA
CCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
SSHDLHRIAVGLGADVPYFLESGGLAYAAGIGEELEDLELTLPFSIVTLFPGDHISTVWA
CCCHHHEEEECCCCCCCHHHHCCCEEEECCCCCCHHHEEEECCEEEEEECCCCCHHHHHH
YRNFYACFEREIPDLKGLVRSLCQARDTSVLEVFDNDFEPVVFDHYPAVREAKDALVQSG
HHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCCHHHHHHHHHHHCC
SFFASLSGSGSAVFGLFENDSDAERAAEQLSATYRARFTPASFAMA
CEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHCCC
>Mature Secondary Structure
MQSISVKAYAKINLGLLITGKRPDGYHTLETVFAPINWYDELTFSPSQEVRMSCTNQDLP
CCCCEEEEEEEEEEEEEEECCCCCCCHHHHHHHHCCCCHHCCCCCCCHHHHEEECCCCCC
CDDSNLCIRAAKALRDFSGVSSGVEIGLAKHIPFGAGLGGGSSDAASTLRVLNRMWNINA
CCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
SSHDLHRIAVGLGADVPYFLESGGLAYAAGIGEELEDLELTLPFSIVTLFPGDHISTVWA
CCCHHHEEEECCCCCCCHHHHCCCEEEECCCCCCHHHEEEECCEEEEEECCCCCHHHHHH
YRNFYACFEREIPDLKGLVRSLCQARDTSVLEVFDNDFEPVVFDHYPAVREAKDALVQSG
HHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCCHHHHHHHHHHHCC
SFFASLSGSGSAVFGLFENDSDAERAAEQLSATYRARFTPASFAMA
CEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA