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The map label for this gene is grpE

Identifier: 194334388

GI number: 194334388

Start: 1724732

End: 1725352

Strand: Reverse

Name: grpE

Synonym: Paes_1583

Alternate gene names: 194334388

Gene position: 1725352-1724732 (Counterclockwise)

Preceding gene: 194334389

Following gene: 194334387

Centisome position: 68.66

GC content: 50.08

Gene sequence:

>621_bases
ATGAGTAAAAAAGCATCAATGCATAAAGAGGAAAAACATGAAAAGGGGCAGCATAAGCAGCCTGCAGATGAACAGCCTCT
CGGCATAGACCCGGAAGTCTCTTCGGGCGAAGCATCCGAAGGAGCTTGTCAGGAGTCGGACGCAAAGGTTCAGGAACTCG
AAAAGGCCCTCGAAGAGGCTCAGCAGCAGGCAGAGAAATATCGTGGTGAAATGATGCGTTTTGCCGCTGATTTCGAGAAT
TTCCGTAAACAGAAAGAGCGGGAGCTTCAAGCAGCAGGAACCCGGAGTATCGAGAATACCATCAGGGAACTGCTTCCTCT
TGTCGATGATATGAAGAGGGTTATGGAGCATGCTCCCGATGATCTGGAGCAGTCCGGGGAGGCTCGTCCGTATTTGGAGG
GTGTTGAACTGTTATGGAAGAATCTTCTGAAATGGTTTGAACGCAAAGGGGTGAAGCAAATCGAGGCTTGCGGACAGAAA
CTGGATGTGAATTTCCATGAAGCCATTACCCAGGTCGATCATCCGGACGCGGAGCCCGATACGGTGATCGAGGAGTATCA
GACCGGCTATGTTATGGGCGATAAAGTGCTGCGGCACGCTAAAGTTATTGTTGCCAGATAA

Upstream 100 bases:

>100_bases
ACGGATGGATTACGCTCGTGTTGTTCGGGTTGTCAATTATATGGCGGACCGTTTGTCAACCACATTTTCAGATGTCAATT
AAATCGTATCAGCACAACTT

Downstream 100 bases:

>100_bases
GAGAGATATATTGTTCTAACTTAAAGGATTTTTTTAAGAGAAGCGATGAAGAGAGATTACTACGAGGTTCTCGGCGTTGG
ACGTTCAGCAACCAAGGACG

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MSKKASMHKEEKHEKGQHKQPADEQPLGIDPEVSSGEASEGACQESDAKVQELEKALEEAQQQAEKYRGEMMRFAADFEN
FRKQKERELQAAGTRSIENTIRELLPLVDDMKRVMEHAPDDLEQSGEARPYLEGVELLWKNLLKWFERKGVKQIEACGQK
LDVNFHEAITQVDHPDAEPDTVIEEYQTGYVMGDKVLRHAKVIVAR

Sequences:

>Translated_206_residues
MSKKASMHKEEKHEKGQHKQPADEQPLGIDPEVSSGEASEGACQESDAKVQELEKALEEAQQQAEKYRGEMMRFAADFEN
FRKQKERELQAAGTRSIENTIRELLPLVDDMKRVMEHAPDDLEQSGEARPYLEGVELLWKNLLKWFERKGVKQIEACGQK
LDVNFHEAITQVDHPDAEPDTVIEEYQTGYVMGDKVLRHAKVIVAR
>Mature_205_residues
SKKASMHKEEKHEKGQHKQPADEQPLGIDPEVSSGEASEGACQESDAKVQELEKALEEAQQQAEKYRGEMMRFAADFENF
RKQKERELQAAGTRSIENTIRELLPLVDDMKRVMEHAPDDLEQSGEARPYLEGVELLWKNLLKWFERKGVKQIEACGQKL
DVNFHEAITQVDHPDAEPDTVIEEYQTGYVMGDKVLRHAKVIVAR

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Escherichia coli, GI1788967, Length=170, Percent_Identity=33.5294117647059, Blast_Score=90, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6324806, Length=165, Percent_Identity=26.0606060606061, Blast_Score=78, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24653432, Length=171, Percent_Identity=29.8245614035088, Blast_Score=83, Evalue=1e-16,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_PROA2 (B4S9D1)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002016248.1
- ProteinModelPortal:   B4S9D1
- SMR:   B4S9D1
- GeneID:   6460163
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_1583
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- ProtClustDB:   CLSK637785
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 23558; Mature: 23426

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKASMHKEEKHEKGQHKQPADEQPLGIDPEVSSGEASEGACQESDAKVQELEKALEEA
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
QQQAEKYRGEMMRFAADFENFRKQKERELQAAGTRSIENTIRELLPLVDDMKRVMEHAPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DLEQSGEARPYLEGVELLWKNLLKWFERKGVKQIEACGQKLDVNFHEAITQVDHPDAEPD
HHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCHH
TVIEEYQTGYVMGDKVLRHAKVIVAR
HHHHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKKASMHKEEKHEKGQHKQPADEQPLGIDPEVSSGEASEGACQESDAKVQELEKALEEA
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
QQQAEKYRGEMMRFAADFENFRKQKERELQAAGTRSIENTIRELLPLVDDMKRVMEHAPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DLEQSGEARPYLEGVELLWKNLLKWFERKGVKQIEACGQKLDVNFHEAITQVDHPDAEPD
HHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCHH
TVIEEYQTGYVMGDKVLRHAKVIVAR
HHHHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA