Click here to switch to the map view.

The map label for this gene is trmB [H]

Identifier: 194333811

GI number: 194333811

Start: 1082973

End: 1083566

Strand: Reverse

Name: trmB [H]

Synonym: Paes_0985

Alternate gene names: 194333811

Gene position: 1083566-1082973 (Counterclockwise)

Preceding gene: 194333815

Following gene: 194333810

Centisome position: 43.12

GC content: 46.8

Gene sequence:

>594_bases
ATGCTCATCGATACCAGAGATCTTCTATCCGATAAGGAGAACATGACAGAAGATAACCAGCACGATGCCAGAGAGATCGA
AGTTGAAATTGGCTTCGGGAATGGAGACTATCTGATCAGAAGAGCGTCAGAATGCCCGGAAAAAATGTTTATCGGTATCG
AAAAAAAAACATCCCTGGTCACAAAAGTCTCAAAAAAAGCCGAAAGCCTGAACCTTTCCAATATCCGGATTCTCCAGTCA
TGCGCTGAAGAGGCCTTCTCCGACCACTTTTCGACCGCTTCGATTTCCAGAGTATATGCGCTCTTTCCCGACCCATGGCC
GAAAAAAAAGCATATCAAAAACAGACTGTTTTCATCACCATACCTCCGGCTGCTCAACAACAGGCTCTTACTCGGCAGCG
AAGCCATGATTGTAACCGACAGCGAAGCGTATCACCGATGGATACTCAAGCAAACCCCGGACACCGGCTTCGACGTCCAA
AACGACACCATCCCACCGCAGTTCGACACCAAATTTGAACGAAAATGGGTAGAACAGGAGTTCAAAAGCTTCTTCCGGAT
CATTCTCAGAAAAATCGAGCACATCGATACCTGA

Upstream 100 bases:

>100_bases
GCCTATTGATGATAAAAAAAAACGGGACAGAAGTAGAGAGGCAGAATCAATCATCTTTTTTCAATACATTGATTCCTTTT
TCCACCGTCAATAATCCACT

Downstream 100 bases:

>100_bases
AACAGTATACCCGACCACGTAACGACAGGTCTCGGTCTGGTTCCTGCTGATGAAGACCAGCGATGCTGTCAATGAGTCCC
ATAAGCAACGCAAGTCTTGT

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MLIDTRDLLSDKENMTEDNQHDAREIEVEIGFGNGDYLIRRASECPEKMFIGIEKKTSLVTKVSKKAESLNLSNIRILQS
CAEEAFSDHFSTASISRVYALFPDPWPKKKHIKNRLFSSPYLRLLNNRLLLGSEAMIVTDSEAYHRWILKQTPDTGFDVQ
NDTIPPQFDTKFERKWVEQEFKSFFRIILRKIEHIDT

Sequences:

>Translated_197_residues
MLIDTRDLLSDKENMTEDNQHDAREIEVEIGFGNGDYLIRRASECPEKMFIGIEKKTSLVTKVSKKAESLNLSNIRILQS
CAEEAFSDHFSTASISRVYALFPDPWPKKKHIKNRLFSSPYLRLLNNRLLLGSEAMIVTDSEAYHRWILKQTPDTGFDVQ
NDTIPPQFDTKFERKWVEQEFKSFFRIILRKIEHIDT
>Mature_197_residues
MLIDTRDLLSDKENMTEDNQHDAREIEVEIGFGNGDYLIRRASECPEKMFIGIEKKTSLVTKVSKKAESLNLSNIRILQS
CAEEAFSDHFSTASISRVYALFPDPWPKKKHIKNRLFSSPYLRLLNNRLLLGSEAMIVTDSEAYHRWILKQTPDTGFDVQ
NDTIPPQFDTKFERKWVEQEFKSFFRIILRKIEHIDT

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=125, Percent_Identity=34.4, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 23014; Mature: 23014

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIDTRDLLSDKENMTEDNQHDAREIEVEIGFGNGDYLIRRASECPEKMFIGIEKKTSLV
CCCCCHHHHCCHHCCCCCCCCCCEEEEEEEECCCCHHHHCCHHHCHHHHEECCCHHHHHH
TKVSKKAESLNLSNIRILQSCAEEAFSDHFSTASISRVYALFPDPWPKKKHIKNRLFSSP
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHEEEECCCCCCCHHHHHHHHCCCH
YLRLLNNRLLLGSEAMIVTDSEAYHRWILKQTPDTGFDVQNDTIPPQFDTKFERKWVEQE
HHHHHCCEEEECCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
FKSFFRIILRKIEHIDT
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLIDTRDLLSDKENMTEDNQHDAREIEVEIGFGNGDYLIRRASECPEKMFIGIEKKTSLV
CCCCCHHHHCCHHCCCCCCCCCCEEEEEEEECCCCHHHHCCHHHCHHHHEECCCHHHHHH
TKVSKKAESLNLSNIRILQSCAEEAFSDHFSTASISRVYALFPDPWPKKKHIKNRLFSSP
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHEEEECCCCCCCHHHHHHHHCCCH
YLRLLNNRLLLGSEAMIVTDSEAYHRWILKQTPDTGFDVQNDTIPPQFDTKFERKWVEQE
HHHHHCCEEEECCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
FKSFFRIILRKIEHIDT
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA