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The map label for this gene is folE [H]

Identifier: 194333692

GI number: 194333692

Start: 941464

End: 942144

Strand: Reverse

Name: folE [H]

Synonym: Paes_0859

Alternate gene names: 194333692

Gene position: 942144-941464 (Counterclockwise)

Preceding gene: 194333693

Following gene: 194333691

Centisome position: 37.49

GC content: 47.72

Gene sequence:

>681_bases
ATGAAACACGAAAAAACTCTGTCAGATACCTTGGCGAATAATCGCCGGTCGTCCTATTCCGGCGTCAGCATGTGCGAAGA
TGATGACTGCCTCGATGCCATGACTGATAACGAACCTCAAATCATTGAAACACTGTCATCTGCGGTTACAACGATTCTTG
GTGGTCTGGGGGAGAACCCTGAGCGCGAAGGACTGCTGAAAACACCGGAACGGGTCGCTAAATCACTGCGTTTTCTGACC
AAAGGCTACCGCGAAAATCCGGAAGAACTGCTTAAAAAAGCAGTCTTTAAAGAATCTTACGACGAAATGGTGCTGGTTAA
GGATATCGAACTCTTTTCGATGTGCGAACATCATATGCTTCCATTTTTCGGAAAAGCGCATGTTGCCTATATTCCTGATG
GAAAGATTGTCGGTCTGTCGAAAATCGCACGTGTCGTGGAAGTCTATGCCCGCCGCCTGCAGGTTCAGGAACGGCTCACC
CAGCAGATAAGGGATGCGATCCAGGACGTGCTTAACCCCAAAGGAGTCGGGGTGATTATCGAAGCCAAGCACATGTGTAT
GGTGATGCGGGGAGTAGAAAAACAAAATTCAGTAACAACAACATCGGCAATGTCCGGCGAATTCATCTCTTCGCCATCCA
CAAGAAGCGAATTCCTTCGCCTTATTCACAACAGATCATAA

Upstream 100 bases:

>100_bases
CCCAGTACGGAAATACTGGCTGTAACTGTCTGGGACATTTTACACGATGCTCTCCAATCATACTCCAACAAGGAGATAAC
TCTTCATGAAGTCAAAATCC

Downstream 100 bases:

>100_bases
CGTTTTACCAGGCCTTTGGTCATGCATCAATCTGCAAGCAACCGCAACCTGCTGGTTGCCGTCATAACAGCCGGAGCCCT
TTTTCTGGGATTTGAAGTCA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MKHEKTLSDTLANNRRSSYSGVSMCEDDDCLDAMTDNEPQIIETLSSAVTTILGGLGENPEREGLLKTPERVAKSLRFLT
KGYRENPEELLKKAVFKESYDEMVLVKDIELFSMCEHHMLPFFGKAHVAYIPDGKIVGLSKIARVVEVYARRLQVQERLT
QQIRDAIQDVLNPKGVGVIIEAKHMCMVMRGVEKQNSVTTTSAMSGEFISSPSTRSEFLRLIHNRS

Sequences:

>Translated_226_residues
MKHEKTLSDTLANNRRSSYSGVSMCEDDDCLDAMTDNEPQIIETLSSAVTTILGGLGENPEREGLLKTPERVAKSLRFLT
KGYRENPEELLKKAVFKESYDEMVLVKDIELFSMCEHHMLPFFGKAHVAYIPDGKIVGLSKIARVVEVYARRLQVQERLT
QQIRDAIQDVLNPKGVGVIIEAKHMCMVMRGVEKQNSVTTTSAMSGEFISSPSTRSEFLRLIHNRS
>Mature_226_residues
MKHEKTLSDTLANNRRSSYSGVSMCEDDDCLDAMTDNEPQIIETLSSAVTTILGGLGENPEREGLLKTPERVAKSLRFLT
KGYRENPEELLKKAVFKESYDEMVLVKDIELFSMCEHHMLPFFGKAHVAYIPDGKIVGLSKIARVVEVYARRLQVQERLT
QQIRDAIQDVLNPKGVGVIIEAKHMCMVMRGVEKQNSVTTTSAMSGEFISSPSTRSEFLRLIHNRS

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=189, Percent_Identity=58.2010582010582, Blast_Score=240, Evalue=6e-64,
Organism=Homo sapiens, GI4503949, Length=189, Percent_Identity=58.2010582010582, Blast_Score=240, Evalue=6e-64,
Organism=Homo sapiens, GI66932970, Length=141, Percent_Identity=57.4468085106383, Blast_Score=190, Evalue=9e-49,
Organism=Homo sapiens, GI66932972, Length=141, Percent_Identity=57.4468085106383, Blast_Score=190, Evalue=1e-48,
Organism=Escherichia coli, GI1788476, Length=185, Percent_Identity=43.7837837837838, Blast_Score=127, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17560486, Length=184, Percent_Identity=57.0652173913043, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6321706, Length=186, Percent_Identity=56.4516129032258, Blast_Score=223, Evalue=3e-59,
Organism=Drosophila melanogaster, GI24656782, Length=198, Percent_Identity=54.5454545454545, Blast_Score=221, Evalue=3e-58,
Organism=Drosophila melanogaster, GI24656791, Length=213, Percent_Identity=51.6431924882629, Blast_Score=221, Evalue=3e-58,
Organism=Drosophila melanogaster, GI24656796, Length=198, Percent_Identity=54.5454545454545, Blast_Score=220, Evalue=5e-58,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 25437; Mature: 25437

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHEKTLSDTLANNRRSSYSGVSMCEDDDCLDAMTDNEPQIIETLSSAVTTILGGLGENP
CCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC
EREGLLKTPERVAKSLRFLTKGYRENPEELLKKAVFKESYDEMVLVKDIELFSMCEHHML
CCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
PFFGKAHVAYIPDGKIVGLSKIARVVEVYARRLQVQERLTQQIRDAIQDVLNPKGVGVII
CCCCCCEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
EAKHMCMVMRGVEKQNSVTTTSAMSGEFISSPSTRSEFLRLIHNRS
HHHHHHHHHHHHHHCCCCCHHHHHCCHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKHEKTLSDTLANNRRSSYSGVSMCEDDDCLDAMTDNEPQIIETLSSAVTTILGGLGENP
CCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC
EREGLLKTPERVAKSLRFLTKGYRENPEELLKKAVFKESYDEMVLVKDIELFSMCEHHML
CCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
PFFGKAHVAYIPDGKIVGLSKIARVVEVYARRLQVQERLTQQIRDAIQDVLNPKGVGVII
CCCCCCEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
EAKHMCMVMRGVEKQNSVTTTSAMSGEFISSPSTRSEFLRLIHNRS
HHHHHHHHHHHHHHCCCCCHHHHHCCHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA