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The map label for this gene is birA [H]

Identifier: 193214348

GI number: 193214348

Start: 738847

End: 739644

Strand: Direct

Name: birA [H]

Synonym: Ctha_0630

Alternate gene names: 193214348

Gene position: 738847-739644 (Clockwise)

Preceding gene: 193214347

Following gene: 193214349

Centisome position: 22.43

GC content: 45.61

Gene sequence:

>798_bases
ATGGACACATCCATTTTTTTGACTGCTGAGAATATTCACAGCCAACTTTCCAGCAATCAGGTGATTGGAAAGAAAGTGTT
TTGCTACCACACCGCCAGCTCAACCAACGAAATTGGACACAAGTTGGCGCGCAGTGGCGCTTTTGATGGCACTTTGATTT
TGGCTGAAACCCAAACCAGGGGGAAAGGTCGCGGCGAACATGTCTGGCTATCAAATCCTGGGGAAAATCTTACTTTTACA
ATTGTACTGCGACCCAATTGTAAACCTGCGCAAACCACCGTGCTCAGCTTAATGGGCTCACTTGCCATTGCCGATGCGAT
CTATAAAATGCACAACAAAAAGCCCGAAATAAAATGGCCAAACGACGTTTTATTAGGCGGGAAAAAAGTTTGTGGATTGT
TGCTCGAATCGGCGATTTCTGGCGAGCAAGCCGATTATGTTTTATTGGGCATTGGTTTGAACGTCAATCAAAAAGTTTTT
CCAAAAGAGATTGAATCCGTAGCAACCTCCTTGCGCTTGGCTGCGAATCGCGCATTTTCGCGCGCAGCAGTCCTTAGCGA
AGTTGCCCGCCATGTTGAAAAACGCTATCTGCAATTTAAAGCCAACAACAGTCAATCGATTCTGGAGGACTGGAAAGCTT
ATTGCACCATGCTTGGCAAAAAAATCACTTTTTTGCACAACGGCATGGAGCGAACAGGCGTCGCCATCGATATTGATGAT
TATGGCTATTTGAGGGTGAGAGTTGGCGCAACGGCTCTCATGCTCTCTCATGCGGAGATTTCTAACGTTAGATTTTAA

Upstream 100 bases:

>100_bases
TACCGAGAAAACCGGTTTAAAAGTGCAACGCTCACTTTAAGCCATTTGCGCGACAGGCTGCTTCTGAAGTTTTGAAACTT
AAGCACATTGACAATTTGCA

Downstream 100 bases:

>100_bases
GCAATGACGCCACGCAACCATTTAGTCAAACAACCCAATTTATCTTCGATCGCAACAGTTGATATTGGAAATACCCAAAC
ATCGATTGCCATTTTCGAAG

Product: biotin/acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MDTSIFLTAENIHSQLSSNQVIGKKVFCYHTASSTNEIGHKLARSGAFDGTLILAETQTRGKGRGEHVWLSNPGENLTFT
IVLRPNCKPAQTTVLSLMGSLAIADAIYKMHNKKPEIKWPNDVLLGGKKVCGLLLESAISGEQADYVLLGIGLNVNQKVF
PKEIESVATSLRLAANRAFSRAAVLSEVARHVEKRYLQFKANNSQSILEDWKAYCTMLGKKITFLHNGMERTGVAIDIDD
YGYLRVRVGATALMLSHAEISNVRF

Sequences:

>Translated_265_residues
MDTSIFLTAENIHSQLSSNQVIGKKVFCYHTASSTNEIGHKLARSGAFDGTLILAETQTRGKGRGEHVWLSNPGENLTFT
IVLRPNCKPAQTTVLSLMGSLAIADAIYKMHNKKPEIKWPNDVLLGGKKVCGLLLESAISGEQADYVLLGIGLNVNQKVF
PKEIESVATSLRLAANRAFSRAAVLSEVARHVEKRYLQFKANNSQSILEDWKAYCTMLGKKITFLHNGMERTGVAIDIDD
YGYLRVRVGATALMLSHAEISNVRF
>Mature_265_residues
MDTSIFLTAENIHSQLSSNQVIGKKVFCYHTASSTNEIGHKLARSGAFDGTLILAETQTRGKGRGEHVWLSNPGENLTFT
IVLRPNCKPAQTTVLSLMGSLAIADAIYKMHNKKPEIKWPNDVLLGGKKVCGLLLESAISGEQADYVLLGIGLNVNQKVF
PKEIESVATSLRLAANRAFSRAAVLSEVARHVEKRYLQFKANNSQSILEDWKAYCTMLGKKITFLHNGMERTGVAIDIDD
YGYLRVRVGATALMLSHAEISNVRF

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Homo sapiens, GI46255045, Length=221, Percent_Identity=27.1493212669683, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790408, Length=243, Percent_Identity=26.7489711934156, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 29166; Mature: 29166

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTSIFLTAENIHSQLSSNQVIGKKVFCYHTASSTNEIGHKLARSGAFDGTLILAETQTR
CCCEEEEEHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCC
GKGRGEHVWLSNPGENLTFTIVLRPNCKPAQTTVLSLMGSLAIADAIYKMHNKKPEIKWP
CCCCCCEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NDVLLGGKKVCGLLLESAISGEQADYVLLGIGLNVNQKVFPKEIESVATSLRLAANRAFS
CCEEECCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RAAVLSEVARHVEKRYLQFKANNSQSILEDWKAYCTMLGKKITFLHNGMERTGVAIDIDD
HHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHCCHHHHHHCCHHHCCEEEEECC
YGYLRVRVGATALMLSHAEISNVRF
CCEEEEEECCEEEEEEHHHHHCCCC
>Mature Secondary Structure
MDTSIFLTAENIHSQLSSNQVIGKKVFCYHTASSTNEIGHKLARSGAFDGTLILAETQTR
CCCEEEEEHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCC
GKGRGEHVWLSNPGENLTFTIVLRPNCKPAQTTVLSLMGSLAIADAIYKMHNKKPEIKWP
CCCCCCEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NDVLLGGKKVCGLLLESAISGEQADYVLLGIGLNVNQKVFPKEIESVATSLRLAANRAFS
CCEEECCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RAAVLSEVARHVEKRYLQFKANNSQSILEDWKAYCTMLGKKITFLHNGMERTGVAIDIDD
HHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHCCHHHHHHCCHHHCCEEEEECC
YGYLRVRVGATALMLSHAEISNVRF
CCEEEEEECCEEEEEEHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]