Click here to switch to the map view.

The map label for this gene is queA [H]

Identifier: 193214235

GI number: 193214235

Start: 612714

End: 613733

Strand: Direct

Name: queA [H]

Synonym: Ctha_0516

Alternate gene names: 193214235

Gene position: 612714-613733 (Clockwise)

Preceding gene: 193214234

Following gene: 193214236

Centisome position: 18.6

GC content: 48.92

Gene sequence:

>1020_bases
ATGAACGTTTCGGATTTTGATTACACGCTACCCGATGAACAAATTGCTAAATATCCGCCAAAAGAGCGAGGAACAACCAA
ACTTTTGGCGCTCAATCGCAAGACGGGCGAAGTTCAGCATAGTTTATATCGCAGCTTGGATGCGTTTTTAGAACCTGGCG
ATTTGCTTGTTTTGAACAACACAAAAGTGATCAAAGCGCGCCTTCAGGCCAATAAGCCAACGGGCGCCAGCGTAGAGCTT
TTGCTATTGGAAAAACATGAAGGGCCACAACAAATCGTTATGTACGGCGGAAAAATTAAGGCTGGAAATACGCTGCTTGT
TGGAGAAGACGAGGTGCTGGTTGAGGAAATTTTGGGAAACGGCTTGGCTAAGGTTTCGGCGGCTGTTCCACTTTTATCGT
TGGTTGAAAAATATGGCAATGTTCCGATTCCACCGTACCTAAATCGCAAAGCCGAAAAAAGCGACGAAATGCGTTATCAG
ACGGTGTTTGCCAAAGAACAAGGCTCGGTGGCTGCACCAACAGCGTCGCTTAATTTCACCGAGTCGCTTGAGAAAAAGCT
CAAGGAAAAAGGCGTGCAACTGGCCTTCTTGACGCTTCATGTTGGGCTGGGAACGTTCATGCCGATTCGCACCGAAACGC
TTTCGGCACATGAAATGCACCGTGAGTTTTATCATATTTCGCATGAAACTGCCGCAGCCATTCGATTGGCAAAAGCCGAA
AAACGACGGGTTGTCGCGCTGGGAACAACGGTGACACGCGCGATCGAGCATGCAAGCGCGCGCCTTTTAGCGCACGAGCC
CGCCGAACTTGCTGGCGAAGCCGACATTTTTATTTATCCAGGCGGATATGAATTTCAAATCGTGGATGCTTTGCTCACAA
ACTTTCACGCGCCACGCTCAACCGTTTTGATGCTGGCTGCCGCGTTTGCCGGCGCAGCGCATCTAAAAGCGGCCTACGAA
GCCGCCCTGCAAAACGACTATCATTTTCTTAGCTACGGCGATAGCATGTTTATTTTCTAA

Upstream 100 bases:

>100_bases
CGGATGACATTTTAAATCGACTGCTGGCTGGCGCAGGCTGGTAGATCGCGCAATCTTAAATTTTGTCAGGAAAAAGAATA
TCAACATTTGTAAGGCAGTC

Downstream 100 bases:

>100_bases
GCCAACGGTGCGCTTCGCCTCGTGAAAAATCTCCCCCAATACAATCCAATTGTGATCTTTTACTGAGCATAAGAGGCTGG
AACAGGGAATCTTCTTTTCA

Product: S-adenosylmethionine/tRNA-ribosyltransferase- isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MNVSDFDYTLPDEQIAKYPPKERGTTKLLALNRKTGEVQHSLYRSLDAFLEPGDLLVLNNTKVIKARLQANKPTGASVEL
LLLEKHEGPQQIVMYGGKIKAGNTLLVGEDEVLVEEILGNGLAKVSAAVPLLSLVEKYGNVPIPPYLNRKAEKSDEMRYQ
TVFAKEQGSVAAPTASLNFTESLEKKLKEKGVQLAFLTLHVGLGTFMPIRTETLSAHEMHREFYHISHETAAAIRLAKAE
KRRVVALGTTVTRAIEHASARLLAHEPAELAGEADIFIYPGGYEFQIVDALLTNFHAPRSTVLMLAAAFAGAAHLKAAYE
AALQNDYHFLSYGDSMFIF

Sequences:

>Translated_339_residues
MNVSDFDYTLPDEQIAKYPPKERGTTKLLALNRKTGEVQHSLYRSLDAFLEPGDLLVLNNTKVIKARLQANKPTGASVEL
LLLEKHEGPQQIVMYGGKIKAGNTLLVGEDEVLVEEILGNGLAKVSAAVPLLSLVEKYGNVPIPPYLNRKAEKSDEMRYQ
TVFAKEQGSVAAPTASLNFTESLEKKLKEKGVQLAFLTLHVGLGTFMPIRTETLSAHEMHREFYHISHETAAAIRLAKAE
KRRVVALGTTVTRAIEHASARLLAHEPAELAGEADIFIYPGGYEFQIVDALLTNFHAPRSTVLMLAAAFAGAAHLKAAYE
AALQNDYHFLSYGDSMFIF
>Mature_339_residues
MNVSDFDYTLPDEQIAKYPPKERGTTKLLALNRKTGEVQHSLYRSLDAFLEPGDLLVLNNTKVIKARLQANKPTGASVEL
LLLEKHEGPQQIVMYGGKIKAGNTLLVGEDEVLVEEILGNGLAKVSAAVPLLSLVEKYGNVPIPPYLNRKAEKSDEMRYQ
TVFAKEQGSVAAPTASLNFTESLEKKLKEKGVQLAFLTLHVGLGTFMPIRTETLSAHEMHREFYHISHETAAAIRLAKAE
KRRVVALGTTVTRAIEHASARLLAHEPAELAGEADIFIYPGGYEFQIVDALLTNFHAPRSTVLMLAAAFAGAAHLKAAYE
AALQNDYHFLSYGDSMFIF

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=345, Percent_Identity=42.0289855072464, Blast_Score=271, Evalue=6e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 37288; Mature: 37288

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVSDFDYTLPDEQIAKYPPKERGTTKLLALNRKTGEVQHSLYRSLDAFLEPGDLLVLNN
CCCCCCCCCCCHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEECC
TKVIKARLQANKPTGASVELLLLEKHEGPQQIVMYGGKIKAGNTLLVGEDEVLVEEILGN
CEEEEEEEECCCCCCCCEEEEEEECCCCCCEEEEECCEEECCCEEEECCHHHHHHHHHCC
GLAKVSAAVPLLSLVEKYGNVPIPPYLNRKAEKSDEMRYQTVFAKEQGSVAAPTASLNFT
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHEEEECCCCCEECCCCCCCHH
ESLEKKLKEKGVQLAFLTLHVGLGTFMPIRTETLSAHEMHREFYHISHETAAAIRLAKAE
HHHHHHHHHCCCEEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
KRRVVALGTTVTRAIEHASARLLAHEPAELAGEADIFIYPGGYEFQIVDALLTNFHAPRS
CCEEEEECHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEECCCCEEEEHHHHHHCCCCCHH
TVLMLAAAFAGAAHLKAAYEAALQNDYHFLSYGDSMFIF
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEC
>Mature Secondary Structure
MNVSDFDYTLPDEQIAKYPPKERGTTKLLALNRKTGEVQHSLYRSLDAFLEPGDLLVLNN
CCCCCCCCCCCHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEECC
TKVIKARLQANKPTGASVELLLLEKHEGPQQIVMYGGKIKAGNTLLVGEDEVLVEEILGN
CEEEEEEEECCCCCCCCEEEEEEECCCCCCEEEEECCEEECCCEEEECCHHHHHHHHHCC
GLAKVSAAVPLLSLVEKYGNVPIPPYLNRKAEKSDEMRYQTVFAKEQGSVAAPTASLNFT
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHEEEECCCCCEECCCCCCCHH
ESLEKKLKEKGVQLAFLTLHVGLGTFMPIRTETLSAHEMHREFYHISHETAAAIRLAKAE
HHHHHHHHHCCCEEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
KRRVVALGTTVTRAIEHASARLLAHEPAELAGEADIFIYPGGYEFQIVDALLTNFHAPRS
CCEEEEECHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEECCCCEEEEHHHHHHCCCCCHH
TVLMLAAAFAGAAHLKAAYEAALQNDYHFLSYGDSMFIF
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA