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The map label for this gene is folD

Identifier: 193214168

GI number: 193214168

Start: 529526

End: 530410

Strand: Direct

Name: folD

Synonym: Ctha_0449

Alternate gene names: 193214168

Gene position: 529526-530410 (Clockwise)

Preceding gene: 193214167

Following gene: 193214172

Centisome position: 16.08

GC content: 45.31

Gene sequence:

>885_bases
ATGGTAGTACTCGATGGAAAAAAGCTTTCACAGGAAATTAAAGCTGAGCTAAAAACAAAAGTTGAGCAGTACAAACAAGA
GATTCAAAAAGTGCCTGGCCTAACGGTTATCATTGTTGGAGAAGATCCGGCCTCGCAAGTCTATGTTCGAAACAAAGCCA
AGTCGTGCAACGAAATCGGCATGGCATCCGAAGTGATTGAACTTCCGGCAAGCACCTCTCAAGAAGAGCTACTCAAAAAA
ATTGCGGATTTGAATCACAATCCCAACGTGCATGGCATTTTAGTGCAGCAGCCTTTGCCGAAGCACATCGATGAGTTCGC
CGTCACGCTGGCCATTGCACCGGAAAAAGATGTCGATGGATTTCATCCTGAAAATGTTGGAAGGCTTGTGCTCGGGCATT
TGGACAAATGTTTTGTAAGCTGCACACCGTTTGGCATCATTGAAATTTTGAAACGCTATAATATTGAAACCAAAGGCAAG
CATTGCGTGATTGTTGGTCGTAGCAATATTGTGGGAAAACCGATGGCAAACTTGATGGTGCAAAAATTGGCGTACATGAA
CTGCACCGTAACCGTTTGCCACTCCGCAACGCCAGACATCGCCACTTACACAAAACAAGCCGATATTTTAATTGCTGCCA
TTGGCAAGGCTCGTTTTATTACAGGCGATATGATTAAAGCAGGCGCTGTGGTAATCGACGTGGGCATTAACCGGATTGAA
GCAACAAACACAAAGAGCGGCTATCGCTTGGTGGGCGATGTTGATTTTGAGGCTGCTTCTCAAAAAGCCAGCGCGATTAC
GCCCGTTCCAGGTGGCGTTGGGCCAATGACAATTTCTATGTTGCTTGCCAACACCATGAAATCCTTCGAGCATTTTTTAG
CTTAA

Upstream 100 bases:

>100_bases
ACCGCGCGGAAGGCTTGCGCAAAACCATTTCATATTTCTTTAAGCAAGCAGGGATTATTTAATCAATTTGAAAAACTTCA
TAAACAGATAGAGAAAATTC

Downstream 100 bases:

>100_bases
AACAGAACTGCTGCCTTTCTAAAGACAGGCAAACTTGATCCTTGAAAAACTTGCTTCTCACAGATTGGGCAACAGGTATG
CGCCAGTCTGTGAGAGCATG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MVVLDGKKLSQEIKAELKTKVEQYKQEIQKVPGLTVIIVGEDPASQVYVRNKAKSCNEIGMASEVIELPASTSQEELLKK
IADLNHNPNVHGILVQQPLPKHIDEFAVTLAIAPEKDVDGFHPENVGRLVLGHLDKCFVSCTPFGIIEILKRYNIETKGK
HCVIVGRSNIVGKPMANLMVQKLAYMNCTVTVCHSATPDIATYTKQADILIAAIGKARFITGDMIKAGAVVIDVGINRIE
ATNTKSGYRLVGDVDFEAASQKASAITPVPGGVGPMTISMLLANTMKSFEHFLA

Sequences:

>Translated_294_residues
MVVLDGKKLSQEIKAELKTKVEQYKQEIQKVPGLTVIIVGEDPASQVYVRNKAKSCNEIGMASEVIELPASTSQEELLKK
IADLNHNPNVHGILVQQPLPKHIDEFAVTLAIAPEKDVDGFHPENVGRLVLGHLDKCFVSCTPFGIIEILKRYNIETKGK
HCVIVGRSNIVGKPMANLMVQKLAYMNCTVTVCHSATPDIATYTKQADILIAAIGKARFITGDMIKAGAVVIDVGINRIE
ATNTKSGYRLVGDVDFEAASQKASAITPVPGGVGPMTISMLLANTMKSFEHFLA
>Mature_294_residues
MVVLDGKKLSQEIKAELKTKVEQYKQEIQKVPGLTVIIVGEDPASQVYVRNKAKSCNEIGMASEVIELPASTSQEELLKK
IADLNHNPNVHGILVQQPLPKHIDEFAVTLAIAPEKDVDGFHPENVGRLVLGHLDKCFVSCTPFGIIEILKRYNIETKGK
HCVIVGRSNIVGKPMANLMVQKLAYMNCTVTVCHSATPDIATYTKQADILIAAIGKARFITGDMIKAGAVVIDVGINRIE
ATNTKSGYRLVGDVDFEAASQKASAITPVPGGVGPMTISMLLANTMKSFEHFLA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI94721354, Length=298, Percent_Identity=49.3288590604027, Blast_Score=254, Evalue=6e-68,
Organism=Homo sapiens, GI222136639, Length=294, Percent_Identity=43.1972789115646, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI222418558, Length=291, Percent_Identity=46.0481099656357, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI36796743, Length=224, Percent_Identity=28.125, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1786741, Length=286, Percent_Identity=47.9020979020979, Blast_Score=265, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17568735, Length=259, Percent_Identity=43.6293436293436, Blast_Score=199, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6319558, Length=285, Percent_Identity=45.6140350877193, Blast_Score=253, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6321643, Length=293, Percent_Identity=42.6621160409556, Blast_Score=240, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6322933, Length=313, Percent_Identity=24.9201277955272, Blast_Score=72, Evalue=8e-14,
Organism=Drosophila melanogaster, GI17136816, Length=292, Percent_Identity=48.972602739726, Blast_Score=271, Evalue=3e-73,
Organism=Drosophila melanogaster, GI17136818, Length=292, Percent_Identity=48.972602739726, Blast_Score=271, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24645718, Length=294, Percent_Identity=46.2585034013605, Blast_Score=258, Evalue=3e-69,
Organism=Drosophila melanogaster, GI17137370, Length=294, Percent_Identity=46.2585034013605, Blast_Score=258, Evalue=3e-69,
Organism=Drosophila melanogaster, GI62472483, Length=294, Percent_Identity=46.2585034013605, Blast_Score=258, Evalue=3e-69,
Organism=Drosophila melanogaster, GI45551871, Length=294, Percent_Identity=46.2585034013605, Blast_Score=258, Evalue=3e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_CHLT3 (B3QUL4)

Other databases:

- EMBL:   CP001100
- RefSeq:   YP_001995367.1
- GeneID:   6421492
- GenomeReviews:   CP001100_GR
- KEGG:   cts:Ctha_0449
- HOGENOM:   HBG328751
- OMA:   CTPLGCI
- ProtClustDB:   PRK14174
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31846; Mature: 31846

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVLDGKKLSQEIKAELKTKVEQYKQEIQKVPGLTVIIVGEDPASQVYVRNKAKSCNEIG
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCCHHHHC
MASEVIELPASTSQEELLKKIADLNHNPNVHGILVQQPLPKHIDEFAVTLAIAPEKDVDG
HHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHEEEEEECCCCCCCC
FHPENVGRLVLGHLDKCFVSCTPFGIIEILKRYNIETKGKHCVIVGRSNIVGKPMANLMV
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHH
QKLAYMNCTVTVCHSATPDIATYTKQADILIAAIGKARFITGDMIKAGAVVIDVGINRIE
HHHHHHCCEEEEECCCCCCHHHHHCCCCEEEEECCCCEEEECCHHHCCEEEEEECCCEEE
ATNTKSGYRLVGDVDFEAASQKASAITPVPGGVGPMTISMLLANTMKSFEHFLA
CCCCCCCEEEEECCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVVLDGKKLSQEIKAELKTKVEQYKQEIQKVPGLTVIIVGEDPASQVYVRNKAKSCNEIG
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCCHHHHC
MASEVIELPASTSQEELLKKIADLNHNPNVHGILVQQPLPKHIDEFAVTLAIAPEKDVDG
HHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHEEEEEECCCCCCCC
FHPENVGRLVLGHLDKCFVSCTPFGIIEILKRYNIETKGKHCVIVGRSNIVGKPMANLMV
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHH
QKLAYMNCTVTVCHSATPDIATYTKQADILIAAIGKARFITGDMIKAGAVVIDVGINRIE
HHHHHHCCEEEEECCCCCCHHHHHCCCCEEEEECCCCEEEECCHHHCCEEEEEECCCEEE
ATNTKSGYRLVGDVDFEAASQKASAITPVPGGVGPMTISMLLANTMKSFEHFLA
CCCCCCCEEEEECCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA