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The map label for this gene is gdhA [H]

Identifier: 193214069

GI number: 193214069

Start: 402453

End: 403760

Strand: Direct

Name: gdhA [H]

Synonym: Ctha_0350

Alternate gene names: 193214069

Gene position: 402453-403760 (Clockwise)

Preceding gene: 193214062

Following gene: 193214074

Centisome position: 12.22

GC content: 49.77

Gene sequence:

>1308_bases
ATGCCGCAAAGACAGCACTCACCGTATTCAAAATCTGTCTACAAAGAGCCCGCGCCAGTACACGACCCCGAAAATCCGTT
TGAATCCATGATGGAGCGCTTTAACCTGGCTGCCGAGTTGCTAAATCTTGATGAAGGGATTTACAATTTTCTGAAAATGC
CAGCCAAACAAGTGATTGTTTCCATTCCGATTCAAATGGATAGCGGCGAAATTAAAGTGTTTGAAGGCTATCGCGTCATT
CACGACGACACGCTCGGCCCGTCAAAAGGCGGCGTGCGCTACGCCCCCGATGTGACGCTGGACGAAGTAAAAGCGCTGGC
CGCCTGGATGACTTGGAAATGCTCGATTTTAGGCTTGCCTTTCGGTGGCGCAAAAGGCGCGGTCAAATGCGATCCGTCAA
AATTGACACCAACTGAATTAGAAAAAATTACCCGTCGCTACACCGCCAGCTTAATTTCCATTTTTGGCCCTGAACGCGAT
ATTCCGGCTCCCGACATGAACACCAACGAGCAAATTATGGCCTGGATTATGGATACCTATTCCATGCACGTAGAACACAC
CGAAACGGCAGTTGTAACAGGCAAACCCGTTATTCTTGGCGGCTCGCTTGGAAGAATTGAAGCAACCGGACGCGGTGTCA
TGATTTCCGCATTGAGCGCTATGAAAAAAATGAATATTTCGCCAGAAAGCGCAAAAGTTGTGGTGCAAGGGTTTGGCAAT
GTTGGTTCTGTTTCTGCCAAACTGCTGGCTGAGCAAGGCTGCAAAATCGTTGGAATCAGCGATATTAGCGGCGGCTATTA
CAACCCAAATGGCATCGATTTGGAGAAAGTTCGCGAACATCTAAAAGTGCATCACGTGCTATCGGGCTTTCCAGAAGCGG
ATTCGGTAACGAACGCCGAATTGCTTGAACTGCCTTGCGATGTGCTGGTGCCCGCTGCAAAGGAAGACCAAATTACCGCA
AAAAATGCAGACAAGTTGAACTGTAAACTGATCGTGGAAGGCGCAAACGGCCCAACCACTGCCGATGCCGACCCAATCTT
AAATGAACGCTGCATCATGGTTGTTCCCGATATTTTGGCCAACGCTGGCGGCGTCACCGTATCTTATTTTGAATGGGTTC
AGGATAGGCAAGGCTACTTCTGGCCGCTTGATCGCGTCAACATGCGCCTCGAACGCTTCATGCGAAACGCTTTCGATACG
GTCTATGAAACCGCTTCTCGCTACAACCAGCCGCTGCGAATCGGCGCGTACATTCAAGCGATCGATAAAGTTGCCAGAAC
CGTACGGCTGCGAGGGATTTACGCCTAA

Upstream 100 bases:

>100_bases
TCTTTGACGAAAGCTTTTTCAATCCGTTCAATTTCGGCTTAAATTAAAAGCAGGTTGAAGTTTTTTTTCTCACCAAAAGT
TTGAGAAGGAGTGATTGATT

Downstream 100 bases:

>100_bases
AGAGTAATTACAAATAGTAATCAATTATTTATTTTGGTAATCACATTCGCATGGCCAATAGCCAGAACACGACATAAATT
CAGCGATCCAATTCTTGGCA

Product: Glu/Leu/Phe/Val dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MPQRQHSPYSKSVYKEPAPVHDPENPFESMMERFNLAAELLNLDEGIYNFLKMPAKQVIVSIPIQMDSGEIKVFEGYRVI
HDDTLGPSKGGVRYAPDVTLDEVKALAAWMTWKCSILGLPFGGAKGAVKCDPSKLTPTELEKITRRYTASLISIFGPERD
IPAPDMNTNEQIMAWIMDTYSMHVEHTETAVVTGKPVILGGSLGRIEATGRGVMISALSAMKKMNISPESAKVVVQGFGN
VGSVSAKLLAEQGCKIVGISDISGGYYNPNGIDLEKVREHLKVHHVLSGFPEADSVTNAELLELPCDVLVPAAKEDQITA
KNADKLNCKLIVEGANGPTTADADPILNERCIMVVPDILANAGGVTVSYFEWVQDRQGYFWPLDRVNMRLERFMRNAFDT
VYETASRYNQPLRIGAYIQAIDKVARTVRLRGIYA

Sequences:

>Translated_435_residues
MPQRQHSPYSKSVYKEPAPVHDPENPFESMMERFNLAAELLNLDEGIYNFLKMPAKQVIVSIPIQMDSGEIKVFEGYRVI
HDDTLGPSKGGVRYAPDVTLDEVKALAAWMTWKCSILGLPFGGAKGAVKCDPSKLTPTELEKITRRYTASLISIFGPERD
IPAPDMNTNEQIMAWIMDTYSMHVEHTETAVVTGKPVILGGSLGRIEATGRGVMISALSAMKKMNISPESAKVVVQGFGN
VGSVSAKLLAEQGCKIVGISDISGGYYNPNGIDLEKVREHLKVHHVLSGFPEADSVTNAELLELPCDVLVPAAKEDQITA
KNADKLNCKLIVEGANGPTTADADPILNERCIMVVPDILANAGGVTVSYFEWVQDRQGYFWPLDRVNMRLERFMRNAFDT
VYETASRYNQPLRIGAYIQAIDKVARTVRLRGIYA
>Mature_434_residues
PQRQHSPYSKSVYKEPAPVHDPENPFESMMERFNLAAELLNLDEGIYNFLKMPAKQVIVSIPIQMDSGEIKVFEGYRVIH
DDTLGPSKGGVRYAPDVTLDEVKALAAWMTWKCSILGLPFGGAKGAVKCDPSKLTPTELEKITRRYTASLISIFGPERDI
PAPDMNTNEQIMAWIMDTYSMHVEHTETAVVTGKPVILGGSLGRIEATGRGVMISALSAMKKMNISPESAKVVVQGFGNV
GSVSAKLLAEQGCKIVGISDISGGYYNPNGIDLEKVREHLKVHHVLSGFPEADSVTNAELLELPCDVLVPAAKEDQITAK
NADKLNCKLIVEGANGPTTADADPILNERCIMVVPDILANAGGVTVSYFEWVQDRQGYFWPLDRVNMRLERFMRNAFDTV
YETASRYNQPLRIGAYIQAIDKVARTVRLRGIYA

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=365, Percent_Identity=45.4794520547945, Blast_Score=305, Evalue=4e-83,
Organism=Homo sapiens, GI4885281, Length=348, Percent_Identity=46.8390804597701, Blast_Score=302, Evalue=3e-82,
Organism=Escherichia coli, GI1788059, Length=382, Percent_Identity=29.3193717277487, Blast_Score=155, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17544676, Length=506, Percent_Identity=35.3754940711462, Blast_Score=278, Evalue=6e-75,
Organism=Saccharomyces cerevisiae, GI6324951, Length=413, Percent_Identity=27.1186440677966, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6319256, Length=391, Percent_Identity=26.8542199488491, Blast_Score=127, Evalue=3e-30,
Organism=Drosophila melanogaster, GI45553475, Length=449, Percent_Identity=36.3028953229399, Blast_Score=282, Evalue=4e-76,
Organism=Drosophila melanogaster, GI45549226, Length=385, Percent_Identity=39.2207792207792, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24649283, Length=343, Percent_Identity=42.2740524781341, Blast_Score=261, Evalue=5e-70,
Organism=Drosophila melanogaster, GI24649460, Length=325, Percent_Identity=39.0769230769231, Blast_Score=230, Evalue=1e-60,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 47907; Mature: 47775

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQRQHSPYSKSVYKEPAPVHDPENPFESMMERFNLAAELLNLDEGIYNFLKMPAKQVIV
CCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEE
SIPIQMDSGEIKVFEGYRVIHDDTLGPSKGGVRYAPDVTLDEVKALAAWMTWKCSILGLP
EEEEEECCCCEEEEECEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHEEEEEEECC
FGGAKGAVKCDPSKLTPTELEKITRRYTASLISIFGPERDIPAPDMNTNEQIMAWIMDTY
CCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
SMHVEHTETAVVTGKPVILGGSLGRIEATGRGVMISALSAMKKMNISPESAKVVVQGFGN
HHEEECCCEEEEECCCEEEECCCCEEEECCCCHHHHHHHHHHHCCCCCHHHEEEEEECCC
VGSVSAKLLAEQGCKIVGISDISGGYYNPNGIDLEKVREHLKVHHVLSGFPEADSVTNAE
CCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCH
LLELPCDVLVPAAKEDQITAKNADKLNCKLIVEGANGPTTADADPILNERCIMVVPDILA
HHHCCHHHCCCCCCCCCEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEECHHHC
NAGGVTVSYFEWVQDRQGYFWPLDRVNMRLERFMRNAFDTVYETASRYNQPLRIGAYIQA
CCCCCEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHH
IDKVARTVRLRGIYA
HHHHHHHHHHCCCCC
>Mature Secondary Structure 
PQRQHSPYSKSVYKEPAPVHDPENPFESMMERFNLAAELLNLDEGIYNFLKMPAKQVIV
CCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEE
SIPIQMDSGEIKVFEGYRVIHDDTLGPSKGGVRYAPDVTLDEVKALAAWMTWKCSILGLP
EEEEEECCCCEEEEECEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHEEEEEEECC
FGGAKGAVKCDPSKLTPTELEKITRRYTASLISIFGPERDIPAPDMNTNEQIMAWIMDTY
CCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
SMHVEHTETAVVTGKPVILGGSLGRIEATGRGVMISALSAMKKMNISPESAKVVVQGFGN
HHEEECCCEEEEECCCEEEECCCCEEEECCCCHHHHHHHHHHHCCCCCHHHEEEEEECCC
VGSVSAKLLAEQGCKIVGISDISGGYYNPNGIDLEKVREHLKVHHVLSGFPEADSVTNAE
CCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCH
LLELPCDVLVPAAKEDQITAKNADKLNCKLIVEGANGPTTADADPILNERCIMVVPDILA
HHHCCHHHCCCCCCCCCEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEECHHHC
NAGGVTVSYFEWVQDRQGYFWPLDRVNMRLERFMRNAFDTVYETASRYNQPLRIGAYIQA
CCCCCEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHH
IDKVARTVRLRGIYA
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]