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The map label for this gene is yrkF [H]

Identifier: 193214053

GI number: 193214053

Start: 382751

End: 383056

Strand: Direct

Name: yrkF [H]

Synonym: Ctha_0334

Alternate gene names: 193214053

Gene position: 382751-383056 (Clockwise)

Preceding gene: 193214041

Following gene: 193214058

Centisome position: 11.62

GC content: 48.69

Gene sequence:

>306_bases
ATGAAACATATCAATCCTGAACAAGCTTTGGAAATAATGAAAATGAGGCCGGAATTCCAAGTGCTTGATGTCAGAACGCG
GCGAGAATTTGGTGTGCGACGGCTCGCTTGTGCGATGAATATTCCGGTCGATGAACTCAGCCGCCGCTACCACGAGCTGG
ATGAAGACGCGGAAATTATTGTTTTGTGCGAGCATGGCCTGAGGAGCCAACGAGCAGCGTTGCTCTTGGAAGATGCTGGA
TTTGAGAAAATTTACAATGTGCTGGGTGGCCTATCGCGTTGGAAAGCACAAACTGTGTCAGGATAG

Upstream 100 bases:

>100_bases
CACCTGAACCAAAGGGTATAGAGCGCACTTCGGCGAACTTTCAGCATGTTTTTTTTGATTAAGCTATGAGTTTCTGAAAA
GAAAAACAGGTTTTTTGAAA

Downstream 100 bases:

>100_bases
TTTATGCCCCGCAACAGGCGTTTAAAGCATGTGCTTGCGAAGCAACGCTTTAACTTCCTCGTCCGTGAGTTCAGGCGAAT
ATTCGACAAGAAATTTTTGT

Product: rhodanese domain-containing protein

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 101; Mature: 101

Protein sequence:

>101_residues
MKHINPEQALEIMKMRPEFQVLDVRTRREFGVRRLACAMNIPVDELSRRYHELDEDAEIIVLCEHGLRSQRAALLLEDAG
FEKIYNVLGGLSRWKAQTVSG

Sequences:

>Translated_101_residues
MKHINPEQALEIMKMRPEFQVLDVRTRREFGVRRLACAMNIPVDELSRRYHELDEDAEIIVLCEHGLRSQRAALLLEDAG
FEKIYNVLGGLSRWKAQTVSG
>Mature_101_residues
MKHINPEQALEIMKMRPEFQVLDVRTRREFGVRRLACAMNIPVDELSRRYHELDEDAEIIVLCEHGLRSQRAALLLEDAG
FEKIYNVLGGLSRWKAQTVSG

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001455 [H]

Pfam domain/function: PF00581 Rhodanese; PF01206 SirA [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 11680; Mature: 11680

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHINPEQALEIMKMRPEFQVLDVRTRREFGVRRLACAMNIPVDELSRRYHELDEDAEII
CCCCCHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEE
VLCEHGLRSQRAALLLEDAGFEKIYNVLGGLSRWKAQTVSG
EEECCCCCCHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCC
>Mature Secondary Structure
MKHINPEQALEIMKMRPEFQVLDVRTRREFGVRRLACAMNIPVDELSRRYHELDEDAEII
CCCCCHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEE
VLCEHGLRSQRAALLLEDAGFEKIYNVLGGLSRWKAQTVSG
EEECCCCCCHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]