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The map label for this gene is trmH [H]

Identifier: 193213878

GI number: 193213878

Start: 187532

End: 188071

Strand: Direct

Name: trmH [H]

Synonym: Ctha_0159

Alternate gene names: 193213878

Gene position: 187532-188071 (Clockwise)

Preceding gene: 193213877

Following gene: 193213886

Centisome position: 5.69

GC content: 50.93

Gene sequence:

>540_bases
ATGTTGAGGAAATTACATGGTTCTGAAATGGGGCGCTTAGAGCCGGACGCGTTTCGCGAAAGCCAAAAACATCCGATTTA
TGTGATGTTTCAAAATATCCGAAGCATGTGGAATGTGGGCTCCATTTTCAGAACATGCGACGCGGCGAGAATTCAGAAAG
TTATTATTACGGGCTACACCGCCACGCCGCCACGCCCGCAAATAGACAAGGTGGCGCTGGGCGCAACCGAAACAGTCGAT
TGGGAATATGTCGCTGACCCGCTTGAGGCGCTCGCGGATTTGAAAGATCAGGGCGTGAAAATCGCCGCGCTGGAAATCAC
CGAAAACGCCCGAAATTATGACGAACTGAGCGCGGAAGATTTTCCACTTTGCCTGGTGTTAGGAAATGAAGTCACGGGCA
TAGACGATGAGGTGCTTCAGGAATGCGATTTTGCGTTGGAAATTCCGCAATACGGCACGAAGCATTCCTTAAATGTGGCC
GTTTCGGCGGGCATCGCGATTTTTGAGGCGGTTCGGCAATTTCGCGCTTGCGACATCTAA

Upstream 100 bases:

>100_bases
AAAATGCCTGCGCATCAATGTGGGAACAAAAGCGGAAAACGACCGATTTTTGTCGGAATTGGAAAAAATTATGAATGAAG
GTTAAGGATTTAGTAGAAAA

Downstream 100 bases:

>100_bases
AAAAAGCCGGACAAAATGACCGTTCCTTGTGAGAACAGTGATTCAATCCGGCTGCAATTTGTTTTCAGTCAAAAAGAGCT
TTCGCAAAGAAAGCGCTTTG

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MLRKLHGSEMGRLEPDAFRESQKHPIYVMFQNIRSMWNVGSIFRTCDAARIQKVIITGYTATPPRPQIDKVALGATETVD
WEYVADPLEALADLKDQGVKIAALEITENARNYDELSAEDFPLCLVLGNEVTGIDDEVLQECDFALEIPQYGTKHSLNVA
VSAGIAIFEAVRQFRACDI

Sequences:

>Translated_179_residues
MLRKLHGSEMGRLEPDAFRESQKHPIYVMFQNIRSMWNVGSIFRTCDAARIQKVIITGYTATPPRPQIDKVALGATETVD
WEYVADPLEALADLKDQGVKIAALEITENARNYDELSAEDFPLCLVLGNEVTGIDDEVLQECDFALEIPQYGTKHSLNVA
VSAGIAIFEAVRQFRACDI
>Mature_179_residues
MLRKLHGSEMGRLEPDAFRESQKHPIYVMFQNIRSMWNVGSIFRTCDAARIQKVIITGYTATPPRPQIDKVALGATETVD
WEYVADPLEALADLKDQGVKIAALEITENARNYDELSAEDFPLCLVLGNEVTGIDDEVLQECDFALEIPQYGTKHSLNVA
VSAGIAIFEAVRQFRACDI

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=169, Percent_Identity=30.7692307692308, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1790623, Length=153, Percent_Identity=30.0653594771242, Blast_Score=67, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 19975; Mature: 19975

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRKLHGSEMGRLEPDAFRESQKHPIYVMFQNIRSMWNVGSIFRTCDAARIQKVIITGYT
CCCCCCCCCCCCCCCHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC
ATPPRPQIDKVALGATETVDWEYVADPLEALADLKDQGVKIAALEITENARNYDELSAED
CCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCCC
FPLCLVLGNEVTGIDDEVLQECDFALEIPQYGTKHSLNVAVSAGIAIFEAVRQFRACDI
CCEEEEECCCCCCCCHHHHHHCCCEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLRKLHGSEMGRLEPDAFRESQKHPIYVMFQNIRSMWNVGSIFRTCDAARIQKVIITGYT
CCCCCCCCCCCCCCCHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC
ATPPRPQIDKVALGATETVDWEYVADPLEALADLKDQGVKIAALEITENARNYDELSAED
CCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCCC
FPLCLVLGNEVTGIDDEVLQECDFALEIPQYGTKHSLNVAVSAGIAIFEAVRQFRACDI
CCEEEEECCCCCCCCHHHHHHCCCEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]