Click here to switch to the map view.

The map label for this gene is cobS [H]

Identifier: 193213827

GI number: 193213827

Start: 126409

End: 127206

Strand: Direct

Name: cobS [H]

Synonym: Ctha_0108

Alternate gene names: 193213827

Gene position: 126409-127206 (Clockwise)

Preceding gene: 193213826

Following gene: 193213828

Centisome position: 3.84

GC content: 48.25

Gene sequence:

>798_bases
GTGAAAACCCAATTCAATGTTTTTTTGTCCGCTGTTATGTTTCTCACCCGTCTGCCGGTTGGCAGATGGGTGCACTACAA
TGCTGAGCATCTGGCCCAATCCTCTATTTACTTTCCATTGGTGGGCGCACTGGTGGGCGCGATTGCGGTAGGCGCGCTGA
GCCTCAGCTTACTGCTTATTCCAGTTCTTCCGGCTGTTCTAATTTCCATGATCGCCACCATTTTAGCAACAGGCGCTTTG
CACGAAGACGGTTTCGCCGATTGCGCCGATGGATTCGGCGGCGGATTCGAGACTGAGCGGGTGCTGGAAATCATGAAAGA
CAGTCGGTTGGGAACTTACGGCGCCATTGCATTGTGGATCAGCTTAAGCTTAAAAGCCGCGTTGCTCTACGAAATTACTT
CTCGAGACGCACTTTTGTGCGCTGGCGCATTAATTAGCGGGCACACGCTTGGAAGATTCTCGAGTTTAGTGTTAATTTAC
CGCTATCCGTATGTGAGAAAAACTGCTTCAAAATCTAAATCGGTCATGAACGGTCTTTCTTCGCCACGGTTAGCAATAGC
TATGATATACACACTCATAATAACTGCCACATCGCTTTTAGAGCAAGCGCTTTTGCCACTTGGCTTTGCAGCTCTTGCAA
CAGGGTTTGCCGGGCGCTACTTCAACCGCCGAATCGGAGGCATTACTGGCGACACGCTCGGTGCAGCCAGCCAATTCGTT
GAACTTTCCGTTTATTTAGGCATCTTAATTCAATCAAATTTTCTTACCACTATTTCTCTTCTATGGAAGTCTTTTTAG

Upstream 100 bases:

>100_bases
CGAAATGGCTACCTTCGACACCGCAGGCGTTTCCGATTCCGAGCAAGATGCGAACAAACAAGCTCGCTCGGCTTCCGAAA
AAAACGATTAACCCGCTACT

Downstream 100 bases:

>100_bases
TTCGTCATCTCGAAATTCCATCTTATGAAAAGGGCGTCTGCGTCGGCCAATCCGACGTTGCATTAACCGAAACGGGTTTG
GCAAACATTCAGCCACTTGC

Product: cobalamin 5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MKTQFNVFLSAVMFLTRLPVGRWVHYNAEHLAQSSIYFPLVGALVGAIAVGALSLSLLLIPVLPAVLISMIATILATGAL
HEDGFADCADGFGGGFETERVLEIMKDSRLGTYGAIALWISLSLKAALLYEITSRDALLCAGALISGHTLGRFSSLVLIY
RYPYVRKTASKSKSVMNGLSSPRLAIAMIYTLIITATSLLEQALLPLGFAALATGFAGRYFNRRIGGITGDTLGAASQFV
ELSVYLGILIQSNFLTTISLLWKSF

Sequences:

>Translated_265_residues
MKTQFNVFLSAVMFLTRLPVGRWVHYNAEHLAQSSIYFPLVGALVGAIAVGALSLSLLLIPVLPAVLISMIATILATGAL
HEDGFADCADGFGGGFETERVLEIMKDSRLGTYGAIALWISLSLKAALLYEITSRDALLCAGALISGHTLGRFSSLVLIY
RYPYVRKTASKSKSVMNGLSSPRLAIAMIYTLIITATSLLEQALLPLGFAALATGFAGRYFNRRIGGITGDTLGAASQFV
ELSVYLGILIQSNFLTTISLLWKSF
>Mature_265_residues
MKTQFNVFLSAVMFLTRLPVGRWVHYNAEHLAQSSIYFPLVGALVGAIAVGALSLSLLLIPVLPAVLISMIATILATGAL
HEDGFADCADGFGGGFETERVLEIMKDSRLGTYGAIALWISLSLKAALLYEITSRDALLCAGALISGHTLGRFSSLVLIY
RYPYVRKTASKSKSVMNGLSSPRLAIAMIYTLIITATSLLEQALLPLGFAALATGFAGRYFNRRIGGITGDTLGAASQFV
ELSVYLGILIQSNFLTTISLLWKSF

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=249, Percent_Identity=34.5381526104418, Blast_Score=101, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 28421; Mature: 28421

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTQFNVFLSAVMFLTRLPVGRWVHYNAEHLAQSSIYFPLVGALVGAIAVGALSLSLLLI
CCCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PVLPAVLISMIATILATGALHEDGFADCADGFGGGFETERVLEIMKDSRLGTYGAIALWI
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHH
SLSLKAALLYEITSRDALLCAGALISGHTLGRFSSLVLIYRYPYVRKTASKSKSVMNGLS
HHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
SPRLAIAMIYTLIITATSLLEQALLPLGFAALATGFAGRYFNRRIGGITGDTLGAASQFV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
ELSVYLGILIQSNFLTTISLLWKSF
HHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTQFNVFLSAVMFLTRLPVGRWVHYNAEHLAQSSIYFPLVGALVGAIAVGALSLSLLLI
CCCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PVLPAVLISMIATILATGALHEDGFADCADGFGGGFETERVLEIMKDSRLGTYGAIALWI
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHH
SLSLKAALLYEITSRDALLCAGALISGHTLGRFSSLVLIYRYPYVRKTASKSKSVMNGLS
HHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
SPRLAIAMIYTLIITATSLLEQALLPLGFAALATGFAGRYFNRRIGGITGDTLGAASQFV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
ELSVYLGILIQSNFLTTISLLWKSF
HHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA