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The map label for this gene is hisF

Identifier: 193213819

GI number: 193213819

Start: 117509

End: 118264

Strand: Direct

Name: hisF

Synonym: Ctha_0100

Alternate gene names: 193213819

Gene position: 117509-118264 (Clockwise)

Preceding gene: 193213817

Following gene: 193213820

Centisome position: 3.57

GC content: 49.21

Gene sequence:

>756_bases
ATGCTTGCAAAACGAATTATCCCTTGCCTTGATGTCAAAAATGGGCGTGTAGTGAAAGGCGTGCAATTTGAAGAATTGCG
CGATGCCGGCTCAATTTTGGAACAAGCAAAATTTTATAATGACGAACTGGCTGACGAGCTTGTTTTTTTAGATATTTCTG
CATCGATCGAGTCGCGGCGAACGACGCTGGAAGAAGTGCTGAAGGTTTCGGAACAGGTGTTTATTCCCCTGACGGTTGGC
GGCGGCATTAATTCCGTTGAACGCGCGCGCGAAGCGTTTTTGCACGGCGCCGACAAAGTTTCCGTGAATACCTCAGCCGT
CAAAGAGCCAACACTGATTTCCGAACTGGCAGAACGATTCGGCTCGCAGGCGGTTGTCGTTGCCATTGATATCAAAAATG
TTGGAAGCCATTATGAGGTTTTCACGCATTCGGGCAAAACGCCAACCGGCCTCGATACGTTGGAATGGGCGCACAAAGTG
GTTGAACTCGGCGCCGGTGAAATTCTTTTGACCAGCATGGACAGAGACGGCACACAAAAAGGCTACGACAATGTGATTTT
GAAGGAAATTTCCACTTCCGTTGGCGTTCCTGTAATTGCATCGGGTGGCGCTGGGAATTTGCAGCATCTTTACGAAGGCT
TTTCCATCGGCATGGCCGATGCGGCGCTGGCCGCCTCGATTTTCCATTTTCGCCAGCATTCGGTTCGTGAGGCAAAAGCA
TTTTTGCAAGAAAAAGGCATTGCGATTCGACTATAA

Upstream 100 bases:

>100_bases
CATGATGCGCTTTTCTCGAACTTCTTTTGAAATTAAAATTGGCAAAAGGAAGCGCAATATTATGACTATGCGTTCTTTTT
AAAAAGAAAACTTTTGAAAA

Downstream 100 bases:

>100_bases
AACAAAACGCTTGCTTTTAAATCAGACATGGATCACATAGACCATTTTTCCCAAACGATATAGAGACGCACTGGCGCGTC
TCTATTATTTATGAGAAAAG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLAKRIIPCLDVKNGRVVKGVQFEELRDAGSILEQAKFYNDELADELVFLDISASIESRRTTLEEVLKVSEQVFIPLTVG
GGINSVERAREAFLHGADKVSVNTSAVKEPTLISELAERFGSQAVVVAIDIKNVGSHYEVFTHSGKTPTGLDTLEWAHKV
VELGAGEILLTSMDRDGTQKGYDNVILKEISTSVGVPVIASGGAGNLQHLYEGFSIGMADAALAASIFHFRQHSVREAKA
FLQEKGIAIRL

Sequences:

>Translated_251_residues
MLAKRIIPCLDVKNGRVVKGVQFEELRDAGSILEQAKFYNDELADELVFLDISASIESRRTTLEEVLKVSEQVFIPLTVG
GGINSVERAREAFLHGADKVSVNTSAVKEPTLISELAERFGSQAVVVAIDIKNVGSHYEVFTHSGKTPTGLDTLEWAHKV
VELGAGEILLTSMDRDGTQKGYDNVILKEISTSVGVPVIASGGAGNLQHLYEGFSIGMADAALAASIFHFRQHSVREAKA
FLQEKGIAIRL
>Mature_251_residues
MLAKRIIPCLDVKNGRVVKGVQFEELRDAGSILEQAKFYNDELADELVFLDISASIESRRTTLEEVLKVSEQVFIPLTVG
GGINSVERAREAFLHGADKVSVNTSAVKEPTLISELAERFGSQAVVVAIDIKNVGSHYEVFTHSGKTPTGLDTLEWAHKV
VELGAGEILLTSMDRDGTQKGYDNVILKEISTSVGVPVIASGGAGNLQHLYEGFSIGMADAALAASIFHFRQHSVREAKA
FLQEKGIAIRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=46.3035019455253, Blast_Score=216, Evalue=9e-58,
Organism=Escherichia coli, GI87082028, Length=245, Percent_Identity=25.3061224489796, Blast_Score=71, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=32.8025477707006, Blast_Score=142, Evalue=7e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_CHLT3 (B3QSI0)

Other databases:

- EMBL:   CP001100
- RefSeq:   YP_001995018.1
- ProteinModelPortal:   B3QSI0
- GeneID:   6421272
- GenomeReviews:   CP001100_GR
- KEGG:   cts:Ctha_0100
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27192; Mature: 27192

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVKNGRVVKGVQFEELRDAGSILEQAKFYNDELADELVFLDISASIESRR
CCHHHCCCCEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHH
TTLEEVLKVSEQVFIPLTVGGGINSVERAREAFLHGADKVSVNTSAVKEPTLISELAERF
HHHHHHHHHHCEEEEEEEECCCCCHHHHHHHHHHCCCCCEEECHHHHCCCHHHHHHHHHH
GSQAVVVAIDIKNVGSHYEVFTHSGKTPTGLDTLEWAHKVVELGAGEILLTSMDRDGTQK
CCCEEEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCC
GYDNVILKEISTSVGVPVIASGGAGNLQHLYEGFSIGMADAALAASIFHFRQHSVREAKA
CHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
FLQEKGIAIRL
HHHHCCCEEEC
>Mature Secondary Structure
MLAKRIIPCLDVKNGRVVKGVQFEELRDAGSILEQAKFYNDELADELVFLDISASIESRR
CCHHHCCCCEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHH
TTLEEVLKVSEQVFIPLTVGGGINSVERAREAFLHGADKVSVNTSAVKEPTLISELAERF
HHHHHHHHHHCEEEEEEEECCCCCHHHHHHHHHHCCCCCEEECHHHHCCCHHHHHHHHHH
GSQAVVVAIDIKNVGSHYEVFTHSGKTPTGLDTLEWAHKVVELGAGEILLTSMDRDGTQK
CCCEEEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCC
GYDNVILKEISTSVGVPVIASGGAGNLQHLYEGFSIGMADAALAASIFHFRQHSVREAKA
CHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
FLQEKGIAIRL
HHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA