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The map label for this gene is mdh

Identifier: 193213817

GI number: 193213817

Start: 114424

End: 115356

Strand: Direct

Name: mdh

Synonym: Ctha_0098

Alternate gene names: 193213817

Gene position: 114424-115356 (Clockwise)

Preceding gene: 193213816

Following gene: 193213819

Centisome position: 3.47

GC content: 46.3

Gene sequence:

>933_bases
ATGAAAATTACAGTAATCGGCGCTGGAAATGTTGGGGCAACGGCAACTCAACGCATTGTTGAAAAACAATTAGCACGTGA
GGTTGTTCTCGTCGATGTTGTCGACGGTGTGCCTCAAGGCAAAGCGTTGGATATGTATGAATCGGCACCTGTTGAGCTTT
TTGATACTCGTGTTGTGGGTACAACTGGGTATGAAGAAACTGCGGGCTCCGATATTATTCTTATCACTGCTGGAAGACCT
CGCAAACCTGGCATGAGTCGAGATGACCTGTTGGCTATGAATACTGAAATTGTGAAAACAGTGACTGAAGAGGCTGTCTC
GAAATCACCGAATGCAATTATTATTGTTGTTTCAAATCCCTTGGATGTGATGACCTACGTGGCTTATGTGAGAAGTGGAT
TTCCGAAAGAGCGGGTAATTGGCATGGCTGGTGTTCTCGATACAGCCCGCTTTCGTACATTTATCGCAATGGAGCTCAAT
GTTTCTGTGCAAGATGTTAATGCCTTTGTTCTGGGAGGACATGGCGATTCAATGGTGCCGGTTGTGAAATACACCACTGT
AGCCGGGATTCCTATTTCGGAACTGCTGCCGCAAGATCGCATTGCTGCACTCGTTGATCGCGCCAGAAAAGGCGGCATTG
AAATTGTCAATTATTTGAAAACCGGCTCAGCTTACTACGCACCATCCGCTTCTGCTGTTGAAATGATCGACGCTATTGTC
AATGACCGCAAGCGCATTATGCCATGTTCGGCGTATGTAACCGGCCAATATGGATTGAACGATGTTTTCGTCGGCGTTCC
TGTAAAATTAGGTCGCGGTGGCGTTGAACAAGTTCTTGAAATCAATTTGGATGAAGCAGATCGCAACGCACTTCAGGCAT
CGGCCAACGAAGTGAAGGAAAGCTGCGAGAAAGTTAATTCCATGATGCAGTAA

Upstream 100 bases:

>100_bases
GTTTTCGGCGATAGAATGCTTTCTTTATGTTATGTTTTTTTACCATCCCCTCAAAAGTTTGACCGTAACGCGTATTTTTT
AACCTTAAATCAAAAACCGC

Downstream 100 bases:

>100_bases
GTTTTTTTCATCCTACGCGAAAATAGCCTTGAGGGAAGAAAGCGTCCGGTATTTATCGGGCGCTTTTTATTTGAGAAAAA
AATTCGAGTCCTCGTTTCGC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MKITVIGAGNVGATATQRIVEKQLAREVVLVDVVDGVPQGKALDMYESAPVELFDTRVVGTTGYEETAGSDIILITAGRP
RKPGMSRDDLLAMNTEIVKTVTEEAVSKSPNAIIIVVSNPLDVMTYVAYVRSGFPKERVIGMAGVLDTARFRTFIAMELN
VSVQDVNAFVLGGHGDSMVPVVKYTTVAGIPISELLPQDRIAALVDRARKGGIEIVNYLKTGSAYYAPSASAVEMIDAIV
NDRKRIMPCSAYVTGQYGLNDVFVGVPVKLGRGGVEQVLEINLDEADRNALQASANEVKESCEKVNSMMQ

Sequences:

>Translated_310_residues
MKITVIGAGNVGATATQRIVEKQLAREVVLVDVVDGVPQGKALDMYESAPVELFDTRVVGTTGYEETAGSDIILITAGRP
RKPGMSRDDLLAMNTEIVKTVTEEAVSKSPNAIIIVVSNPLDVMTYVAYVRSGFPKERVIGMAGVLDTARFRTFIAMELN
VSVQDVNAFVLGGHGDSMVPVVKYTTVAGIPISELLPQDRIAALVDRARKGGIEIVNYLKTGSAYYAPSASAVEMIDAIV
NDRKRIMPCSAYVTGQYGLNDVFVGVPVKLGRGGVEQVLEINLDEADRNALQASANEVKESCEKVNSMMQ
>Mature_310_residues
MKITVIGAGNVGATATQRIVEKQLAREVVLVDVVDGVPQGKALDMYESAPVELFDTRVVGTTGYEETAGSDIILITAGRP
RKPGMSRDDLLAMNTEIVKTVTEEAVSKSPNAIIIVVSNPLDVMTYVAYVRSGFPKERVIGMAGVLDTARFRTFIAMELN
VSVQDVNAFVLGGHGDSMVPVVKYTTVAGIPISELLPQDRIAALVDRARKGGIEIVNYLKTGSAYYAPSASAVEMIDAIV
NDRKRIMPCSAYVTGQYGLNDVFVGVPVKLGRGGVEQVLEINLDEADRNALQASANEVKESCEKVNSMMQ

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=307, Percent_Identity=34.8534201954397, Blast_Score=191, Evalue=8e-49,
Organism=Homo sapiens, GI221136809, Length=307, Percent_Identity=34.8534201954397, Blast_Score=191, Evalue=8e-49,
Organism=Homo sapiens, GI260099723, Length=303, Percent_Identity=34.3234323432343, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI5031857, Length=303, Percent_Identity=34.3234323432343, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI15082234, Length=307, Percent_Identity=32.5732899022801, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI291575128, Length=302, Percent_Identity=36.0927152317881, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI4557032, Length=302, Percent_Identity=36.0927152317881, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI9257228, Length=302, Percent_Identity=32.1192052980132, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI4504973, Length=302, Percent_Identity=32.1192052980132, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI260099725, Length=196, Percent_Identity=41.3265306122449, Blast_Score=148, Evalue=8e-36,
Organism=Homo sapiens, GI260099727, Length=196, Percent_Identity=41.3265306122449, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI207028494, Length=189, Percent_Identity=32.8042328042328, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI103472011, Length=301, Percent_Identity=27.906976744186, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI21735621, Length=322, Percent_Identity=29.1925465838509, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI103472015, Length=179, Percent_Identity=27.9329608938547, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI1789632, Length=325, Percent_Identity=28.6153846153846, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17535107, Length=291, Percent_Identity=34.020618556701, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17554310, Length=317, Percent_Identity=27.1293375394322, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6322765, Length=224, Percent_Identity=29.4642857142857, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320125, Length=278, Percent_Identity=27.6978417266187, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17136226, Length=303, Percent_Identity=32.3432343234323, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI45550422, Length=310, Percent_Identity=30.9677419354839, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24647881, Length=322, Percent_Identity=29.8136645962733, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24663599, Length=251, Percent_Identity=31.0756972111554, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24663595, Length=251, Percent_Identity=27.8884462151394, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_CHLT3 (B3QSH8)

Other databases:

- EMBL:   CP001100
- RefSeq:   YP_001995016.1
- ProteinModelPortal:   B3QSH8
- GeneID:   6421270
- GenomeReviews:   CP001100_GR
- KEGG:   cts:Ctha_0098
- HOGENOM:   HBG566126
- OMA:   YAVYKES
- ProtClustDB:   PRK06223
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 33272; Mature: 33272

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: ACT_SITE 174-174 BINDING 32-32 BINDING 81-81 BINDING 87-87 BINDING 94-94 BINDING 119-119 BINDING 150-150

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITVIGAGNVGATATQRIVEKQLAREVVLVDVVDGVPQGKALDMYESAPVELFDTRVVG
CEEEEEECCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCEECCCCCCCCEEEEEEEEE
TTGYEETAGSDIILITAGRPRKPGMSRDDLLAMNTEIVKTVTEEAVSKSPNAIIIVVSNP
CCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCC
LDVMTYVAYVRSGFPKERVIGMAGVLDTARFRTFIAMELNVSVQDVNAFVLGGHGDSMVP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEEEEEEEEEEEEEEEEECCCCCCCCE
VVKYTTVAGIPISELLPQDRIAALVDRARKGGIEIVNYLKTGSAYYAPSASAVEMIDAIV
EEEEEEECCCCHHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCCEEECCCCHHHHHHHHHH
NDRKRIMPCSAYVTGQYGLNDVFVGVPVKLGRGGVEQVLEINLDEADRNALQASANEVKE
CCCHHEEEEEEEEECCCCCCEEEEECCCEECCCCHHHHHEECCCHHHHHHHHHHHHHHHH
SCEKVNSMMQ
HHHHHHHHCC
>Mature Secondary Structure
MKITVIGAGNVGATATQRIVEKQLAREVVLVDVVDGVPQGKALDMYESAPVELFDTRVVG
CEEEEEECCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCEECCCCCCCCEEEEEEEEE
TTGYEETAGSDIILITAGRPRKPGMSRDDLLAMNTEIVKTVTEEAVSKSPNAIIIVVSNP
CCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCC
LDVMTYVAYVRSGFPKERVIGMAGVLDTARFRTFIAMELNVSVQDVNAFVLGGHGDSMVP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEEEEEEEEEEEEEEEEECCCCCCCCE
VVKYTTVAGIPISELLPQDRIAALVDRARKGGIEIVNYLKTGSAYYAPSASAVEMIDAIV
EEEEEEECCCCHHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCCEEECCCCHHHHHHHHHH
NDRKRIMPCSAYVTGQYGLNDVFVGVPVKLGRGGVEQVLEINLDEADRNALQASANEVKE
CCCHHEEEEEEEEECCCCCCEEEEECCCEECCCCHHHHHEECCCHHHHHHHHHHHHHHHH
SCEKVNSMMQ
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA