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The map label for this gene is hemK [H]

Identifier: 193213806

GI number: 193213806

Start: 101066

End: 101950

Strand: Direct

Name: hemK [H]

Synonym: Ctha_0087

Alternate gene names: 193213806

Gene position: 101066-101950 (Clockwise)

Preceding gene: 193213802

Following gene: 193213807

Centisome position: 3.07

GC content: 45.42

Gene sequence:

>885_bases
ATGTCTCAATCGCAAAACTGGACGGTTCTTTCGCTCTTAAAAGCAAGCAGCGATTTTTTTGCACAAAAAAATATCGACGA
CGCGCGACTCAATGCCGAATTGCTCTTGGCGCATACGCTGAACCTGAAACGAATGGACTTGTATTTGAAATTCGACATGC
CCGTCACGGAACAGGAGCGCCAAACCTTTCGCGAACTTTGCAAACGGCGACTCGAAGGCGAGCCCGTTCAATACATCATT
GGCAATCAAGATTTTTTTGGACTGACGCTCGACGTGGATTCGCGCGTTCTGATTCCACGCCCCGAAACGGAACTGCTCGT
TGAAGAGGCGCTCAACAGCCTTTCGCAGTTGGATTTCGGGGACGAGAAAATCAAGATTTTGGACATCGGCACGGGAAGCG
GGTGCATTGCGCTGGCGTTTGCCTCGCAGCTCTCGAACGCGGAAATTCTTGCCGTTGATGTTTCTTCGGAGGCGCTTGCG
CTGGCAAAACAAAATTCAGAAAAAAATAAACTTAAGTCTGAAGTCCGTTTTTTGAACATAGATATGCTTTCAGCGCATTT
TTACGATGAGGTTCCAGGAAGCTATCATTTGATTATCTCCAATCCTCCTTATATTCCAATAGCAGAGCGCGATTCACTTC
AAGTGGAGGTGAGAAATTTTGAACCGGCAATTGCACTATTTGTTCAGCAAGGATTCGAGTTTTACGAAAAAATCGCTCAA
GAGGCTGCCAGGTTGCTCAAGCCAAACGGCCTTTTGTGCTTTGAACTCCACGCGGATGGCGCCACAAAAGTGAATATCAT
TTTAAAAAAAAATGGTTTTGAACAGATCCGTTTTGTTCAAGATTATGCTGGTTTTTCGAGAATTGCAATTGCCAGTAATT
CTTAA

Upstream 100 bases:

>100_bases
ATAAAAATTTGGAAGCCTATTTTTTGAAACTCACATTTCTTTTGATAGGCTGGTTTGAAAATTAAAATTAGCATTCACTC
ATTTTTCTCGAACGATAAAT

Downstream 100 bases:

>100_bases
TACACCCAGATAATGAACAGCAAAATTATTGGAATTGTCGGCGCAAAAAGTAGTGGCGTCAATATGGCCATTACAGTCGC
AAAGGCAAACCACAGCGTGC

Product: protein-(glutamine-N5) methyltransferase, release factor-specific

Products: NA

Alternate protein names: M.HindHemKP [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MSQSQNWTVLSLLKASSDFFAQKNIDDARLNAELLLAHTLNLKRMDLYLKFDMPVTEQERQTFRELCKRRLEGEPVQYII
GNQDFFGLTLDVDSRVLIPRPETELLVEEALNSLSQLDFGDEKIKILDIGTGSGCIALAFASQLSNAEILAVDVSSEALA
LAKQNSEKNKLKSEVRFLNIDMLSAHFYDEVPGSYHLIISNPPYIPIAERDSLQVEVRNFEPAIALFVQQGFEFYEKIAQ
EAARLLKPNGLLCFELHADGATKVNIILKKNGFEQIRFVQDYAGFSRIAIASNS

Sequences:

>Translated_294_residues
MSQSQNWTVLSLLKASSDFFAQKNIDDARLNAELLLAHTLNLKRMDLYLKFDMPVTEQERQTFRELCKRRLEGEPVQYII
GNQDFFGLTLDVDSRVLIPRPETELLVEEALNSLSQLDFGDEKIKILDIGTGSGCIALAFASQLSNAEILAVDVSSEALA
LAKQNSEKNKLKSEVRFLNIDMLSAHFYDEVPGSYHLIISNPPYIPIAERDSLQVEVRNFEPAIALFVQQGFEFYEKIAQ
EAARLLKPNGLLCFELHADGATKVNIILKKNGFEQIRFVQDYAGFSRIAIASNS
>Mature_293_residues
SQSQNWTVLSLLKASSDFFAQKNIDDARLNAELLLAHTLNLKRMDLYLKFDMPVTEQERQTFRELCKRRLEGEPVQYIIG
NQDFFGLTLDVDSRVLIPRPETELLVEEALNSLSQLDFGDEKIKILDIGTGSGCIALAFASQLSNAEILAVDVSSEALAL
AKQNSEKNKLKSEVRFLNIDMLSAHFYDEVPGSYHLIISNPPYIPIAERDSLQVEVRNFEPAIALFVQQGFEFYEKIAQE
AARLLKPNGLLCFELHADGATKVNIILKKNGFEQIRFVQDYAGFSRIAIASNS

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=259, Percent_Identity=35.1351351351351, Blast_Score=141, Evalue=6e-34,
Organism=Escherichia coli, GI1787463, Length=291, Percent_Identity=31.6151202749141, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI87082085, Length=220, Percent_Identity=35.9090909090909, Blast_Score=100, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324266, Length=205, Percent_Identity=35.1219512195122, Blast_Score=102, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24582226, Length=268, Percent_Identity=27.9850746268657, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 33109; Mature: 32978

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSQNWTVLSLLKASSDFFAQKNIDDARLNAELLLAHTLNLKRMDLYLKFDMPVTEQER
CCCCCCCEEEEEECCCCHHHHHCCCCHHHCCCEEEEEEECCEEEEEEEEEECCCCCHHHH
QTFRELCKRRLEGEPVQYIIGNQDFFGLTLDVDSRVLIPRPETELLVEEALNSLSQLDFG
HHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCEEEECCCCHHHHHHHHHHHHHHCCCC
DEKIKILDIGTGSGCIALAFASQLSNAEILAVDVSSEALALAKQNSEKNKLKSEVRFLNI
CCEEEEEEECCCCCCEEEEEHHHCCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHEEEEE
DMLSAHFYDEVPGSYHLIISNPPYIPIAERDSLQVEVRNFEPAIALFVQQGFEFYEKIAQ
EEEEHHHHHCCCCCEEEEECCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
EAARLLKPNGLLCFELHADGATKVNIILKKNGFEQIRFVQDYAGFSRIAIASNS
HHHHHHCCCCEEEEEEECCCCEEEEEEEECCCHHHHHHHHHHCCCEEEEEECCC
>Mature Secondary Structure 
SQSQNWTVLSLLKASSDFFAQKNIDDARLNAELLLAHTLNLKRMDLYLKFDMPVTEQER
CCCCCCEEEEEECCCCHHHHHCCCCHHHCCCEEEEEEECCEEEEEEEEEECCCCCHHHH
QTFRELCKRRLEGEPVQYIIGNQDFFGLTLDVDSRVLIPRPETELLVEEALNSLSQLDFG
HHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCEEEECCCCHHHHHHHHHHHHHHCCCC
DEKIKILDIGTGSGCIALAFASQLSNAEILAVDVSSEALALAKQNSEKNKLKSEVRFLNI
CCEEEEEEECCCCCCEEEEEHHHCCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHEEEEE
DMLSAHFYDEVPGSYHLIISNPPYIPIAERDSLQVEVRNFEPAIALFVQQGFEFYEKIAQ
EEEEHHHHHCCCCCEEEEECCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
EAARLLKPNGLLCFELHADGATKVNIILKKNGFEQIRFVQDYAGFSRIAIASNS
HHHHHHCCCCEEEEEEECCCCEEEEEEEECCCHHHHHHHHHHCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]