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The map label for this gene is amiA [H]

Identifier: 193213787

GI number: 193213787

Start: 79601

End: 81391

Strand: Direct

Name: amiA [H]

Synonym: Ctha_0068

Alternate gene names: 193213787

Gene position: 79601-81391 (Clockwise)

Preceding gene: 193213782

Following gene: 193213788

Centisome position: 2.42

GC content: 46.62

Gene sequence:

>1791_bases
ATGGTTTTTCATCAAAGAAGCTTGAAAAATAAGATCGCGGCGCACGCCCAGCCGGCCTTTGCTGAAGGCGTTTTTTCCAC
AGGCGATTGCGACAAAAAGCAACACAACTTGGCGCTCCTCACCCTATTCCTTTTTCTTACACTTTTACTTCTTTTACCGC
AAAACGCATTGGCGGGCGAAACCATCAGCGTTTCGGTCACTTTAGAAAACAAGCAGGTTTATACAAAAAAAATCGCGCTT
TATAGCGTCGGAAATCTGAAAATGGTGGCGGTTTCAGAACTTTGCGACGCCTTGCGGCTTTCGAACACGCAAAGCTTGGC
CGATTTGGTCGTCACGGCAAAACCGGAAAGTCAGCGCGAGTTTTGCACGTTTAGGCCGCTTAATCATTTTGCCGTGATTC
GCTCGATGGAAGGCAGCAAACCTGCACACGTGTTGCAACTTGGTGTTGCGCCAGCTATGATTGGCGGCACGATTTATCTT
CCGCCGGCGCAAGTTGCTCGGCTTTTTTCATCGTGGCAAGGGCTAAAATTTGAATACAATTTGGTAACCAAAATTTTTAC
GGCTGACTTTTCGCAAAAACTTTCGGCCTCGATTGCAAAATCACCTACGCCGCCCAGCCTTTCAGAAAAAGATGAAAAGG
AAAGCTTAACGCTCGACGAACAAGACAAAGTCGATACGCGATTTACGATTCCAAAATTCAGCATCGATGAAAAAGCAAAC
GGCGCAATTATCCGCATTTATTGCACGCGCCCAGAGGTCGAATATGAATTCATCCGCCCGAATAAAAACGGGGTCGCGTA
TTTGACTTTCCGAAACGCCGTTGGCGATATCAAAAACCTAACGCAGACTTTTAGCACAGGATTACTAAAGGAAGTGACTG
CGTTTGCGTTGCGTTCCGGCGGCTTGCAACTGACGCTGAACTTTAACACGAAACGCTACAAGATTAAATCGACCGAATGC
AAACGCGACGAAAAAAGCGATGATTTTCTGGTGCATGTTCTCAGCGATGTGGATGTTTCGGAAATTTACAAGACGGAAAA
AGAGAAGGAAATCAAAGCGCTGCTGCAAGAAGATCGCGAACGATGGAAACTGGACGTTATCGCACTCGACGCAGGCCATG
GCGGGAAAGATCCTGGCGCGATTGGCTATTCTGGCACGTACGAAAAAAATGTGGTGCTCGGCGTGGTGATGGAACTTGGG
AAATTGATAAACCAATATTGGCCAGATGTGAAAGTGGTTTATACCCGAAAAACAGACGACTTTATCGAGCTCGACGAGCG
AGGAAGAATTGCCAACCAGCAAAATGCCAAGCTTTTTGTGAGCGTGCATTGCAATGCGAGCCGCAATCAGCGAGTTTCAG
GTGTGGAAGTCTATATGCTCGGCTTGCATAAAACCGATGCGGCACTTCGCGTGGCTGAGCGCGAAAACGCCGTTATTTTG
CAAGAAGATGATTACAAGGATCGCTATAAAGATTTCACTGACGAGAACCTGATTATGATCACCATGGCACAAAGTGCGTT
TTCGTATCAGTCGCAGAAACTGGCTGATTTGATCAATAGAAATATCCAAGAAAGAACGAAACAGGCTGGCAGAGGTGTTA
AACAAGCTGGATTTATGGTGCTTTGGACGCCTTCAATGCCAAGCGTTTTGGTTGAGGCTGGCTACATTACCAATCCTCGC
GAAGAGCGTTTCCTAAAATCTAAAGAAGGCCAGCTACGAGTGGCTCGCGCTATTTTTGAAGGCCTCAAAAAATATCGATC
GGATTATGAAGCGCAATTAAAAGCGCGGTAA

Upstream 100 bases:

>100_bases
AAGAGCGAAGCGCAAAAGATGAGCCGGCACGCGGTAAATAGCAAGCACTTCATGGTTCGGATTGAGATTGGATGGATTAT
AAAAGACTTTTGAAAATTTA

Downstream 100 bases:

>100_bases
AAACCCGATTTAGATTGCCCAGAAAATTCCAGAAAAGGCCATCGCATAGGTTACAAAATGTTCAAGCATTTTTTATTTTT
ACCCTATTGTTAACATAGAG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MVFHQRSLKNKIAAHAQPAFAEGVFSTGDCDKKQHNLALLTLFLFLTLLLLLPQNALAGETISVSVTLENKQVYTKKIAL
YSVGNLKMVAVSELCDALRLSNTQSLADLVVTAKPESQREFCTFRPLNHFAVIRSMEGSKPAHVLQLGVAPAMIGGTIYL
PPAQVARLFSSWQGLKFEYNLVTKIFTADFSQKLSASIAKSPTPPSLSEKDEKESLTLDEQDKVDTRFTIPKFSIDEKAN
GAIIRIYCTRPEVEYEFIRPNKNGVAYLTFRNAVGDIKNLTQTFSTGLLKEVTAFALRSGGLQLTLNFNTKRYKIKSTEC
KRDEKSDDFLVHVLSDVDVSEIYKTEKEKEIKALLQEDRERWKLDVIALDAGHGGKDPGAIGYSGTYEKNVVLGVVMELG
KLINQYWPDVKVVYTRKTDDFIELDERGRIANQQNAKLFVSVHCNASRNQRVSGVEVYMLGLHKTDAALRVAERENAVIL
QEDDYKDRYKDFTDENLIMITMAQSAFSYQSQKLADLINRNIQERTKQAGRGVKQAGFMVLWTPSMPSVLVEAGYITNPR
EERFLKSKEGQLRVARAIFEGLKKYRSDYEAQLKAR

Sequences:

>Translated_596_residues
MVFHQRSLKNKIAAHAQPAFAEGVFSTGDCDKKQHNLALLTLFLFLTLLLLLPQNALAGETISVSVTLENKQVYTKKIAL
YSVGNLKMVAVSELCDALRLSNTQSLADLVVTAKPESQREFCTFRPLNHFAVIRSMEGSKPAHVLQLGVAPAMIGGTIYL
PPAQVARLFSSWQGLKFEYNLVTKIFTADFSQKLSASIAKSPTPPSLSEKDEKESLTLDEQDKVDTRFTIPKFSIDEKAN
GAIIRIYCTRPEVEYEFIRPNKNGVAYLTFRNAVGDIKNLTQTFSTGLLKEVTAFALRSGGLQLTLNFNTKRYKIKSTEC
KRDEKSDDFLVHVLSDVDVSEIYKTEKEKEIKALLQEDRERWKLDVIALDAGHGGKDPGAIGYSGTYEKNVVLGVVMELG
KLINQYWPDVKVVYTRKTDDFIELDERGRIANQQNAKLFVSVHCNASRNQRVSGVEVYMLGLHKTDAALRVAERENAVIL
QEDDYKDRYKDFTDENLIMITMAQSAFSYQSQKLADLINRNIQERTKQAGRGVKQAGFMVLWTPSMPSVLVEAGYITNPR
EERFLKSKEGQLRVARAIFEGLKKYRSDYEAQLKAR
>Mature_596_residues
MVFHQRSLKNKIAAHAQPAFAEGVFSTGDCDKKQHNLALLTLFLFLTLLLLLPQNALAGETISVSVTLENKQVYTKKIAL
YSVGNLKMVAVSELCDALRLSNTQSLADLVVTAKPESQREFCTFRPLNHFAVIRSMEGSKPAHVLQLGVAPAMIGGTIYL
PPAQVARLFSSWQGLKFEYNLVTKIFTADFSQKLSASIAKSPTPPSLSEKDEKESLTLDEQDKVDTRFTIPKFSIDEKAN
GAIIRIYCTRPEVEYEFIRPNKNGVAYLTFRNAVGDIKNLTQTFSTGLLKEVTAFALRSGGLQLTLNFNTKRYKIKSTEC
KRDEKSDDFLVHVLSDVDVSEIYKTEKEKEIKALLQEDRERWKLDVIALDAGHGGKDPGAIGYSGTYEKNVVLGVVMELG
KLINQYWPDVKVVYTRKTDDFIELDERGRIANQQNAKLFVSVHCNASRNQRVSGVEVYMLGLHKTDAALRVAERENAVIL
QEDDYKDRYKDFTDENLIMITMAQSAFSYQSQKLADLINRNIQERTKQAGRGVKQAGFMVLWTPSMPSVLVEAGYITNPR
EERFLKSKEGQLRVARAIFEGLKKYRSDYEAQLKAR

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm. Note=Distributed throughout the periplasm in all cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=224, Percent_Identity=35.2678571428571, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI87082163, Length=227, Percent_Identity=39.2070484581498, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI1790611, Length=231, Percent_Identity=35.0649350649351, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 67132; Mature: 67132

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFHQRSLKNKIAAHAQPAFAEGVFSTGDCDKKQHNLALLTLFLFLTLLLLLPQNALAGE
CCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TISVSVTLENKQVYTKKIALYSVGNLKMVAVSELCDALRLSNTQSLADLVVTAKPESQRE
EEEEEEEECCCEEEEEEEEEEEECCEEEEEHHHHHHHHHCCCCHHHHHEEEECCCCCCCC
FCTFRPLNHFAVIRSMEGSKPAHVLQLGVAPAMIGGTIYLPPAQVARLFSSWQGLKFEYN
CEEECCCHHHHHEEECCCCCCCEEEEECCCHHHHCCEEEECHHHHHHHHHHCCCCEEEEE
LVTKIFTADFSQKLSASIAKSPTPPSLSEKDEKESLTLDEQDKVDTRFTIPKFSIDEKAN
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCEEECCCCCCCCCCC
GAIIRIYCTRPEVEYEFIRPNKNGVAYLTFRNAVGDIKNLTQTFSTGLLKEVTAFALRSG
CCEEEEEECCCCCEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GLQLTLNFNTKRYKIKSTECKRDEKSDDFLVHVLSDVDVSEIYKTEKEKEIKALLQEDRE
CEEEEEEECCEEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
RWKLDVIALDAGHGGKDPGAIGYSGTYEKNVVLGVVMELGKLINQYWPDVKVVYTRKTDD
HEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
FIELDERGRIANQQNAKLFVSVHCNASRNQRVSGVEVYMLGLHKTDAALRVAERENAVIL
CEEECCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEEEEEECCCHHEEEECCCCEEEE
QEDDYKDRYKDFTDENLIMITMAQSAFSYQSQKLADLINRNIQERTKQAGRGVKQAGFMV
ECCCCHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHCCEEE
LWTPSMPSVLVEAGYITNPREERFLKSKEGQLRVARAIFEGLKKYRSDYEAQLKAR
EECCCCCHHHEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEECCC
>Mature Secondary Structure
MVFHQRSLKNKIAAHAQPAFAEGVFSTGDCDKKQHNLALLTLFLFLTLLLLLPQNALAGE
CCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TISVSVTLENKQVYTKKIALYSVGNLKMVAVSELCDALRLSNTQSLADLVVTAKPESQRE
EEEEEEEECCCEEEEEEEEEEEECCEEEEEHHHHHHHHHCCCCHHHHHEEEECCCCCCCC
FCTFRPLNHFAVIRSMEGSKPAHVLQLGVAPAMIGGTIYLPPAQVARLFSSWQGLKFEYN
CEEECCCHHHHHEEECCCCCCCEEEEECCCHHHHCCEEEECHHHHHHHHHHCCCCEEEEE
LVTKIFTADFSQKLSASIAKSPTPPSLSEKDEKESLTLDEQDKVDTRFTIPKFSIDEKAN
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCEEECCCCCCCCCCC
GAIIRIYCTRPEVEYEFIRPNKNGVAYLTFRNAVGDIKNLTQTFSTGLLKEVTAFALRSG
CCEEEEEECCCCCEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GLQLTLNFNTKRYKIKSTECKRDEKSDDFLVHVLSDVDVSEIYKTEKEKEIKALLQEDRE
CEEEEEEECCEEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
RWKLDVIALDAGHGGKDPGAIGYSGTYEKNVVLGVVMELGKLINQYWPDVKVVYTRKTDD
HEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
FIELDERGRIANQQNAKLFVSVHCNASRNQRVSGVEVYMLGLHKTDAALRVAERENAVIL
CEEECCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEEEEEECCCHHEEEECCCCEEEE
QEDDYKDRYKDFTDENLIMITMAQSAFSYQSQKLADLINRNIQERTKQAGRGVKQAGFMV
ECCCCHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHCCEEE
LWTPSMPSVLVEAGYITNPREERFLKSKEGQLRVARAIFEGLKKYRSDYEAQLKAR
EECCCCCHHHEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503; 1903834 [H]