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The map label for this gene is clpB1 [H]

Identifier: 193213633

GI number: 193213633

Start: 2197075

End: 2198391

Strand: Direct

Name: clpB1 [H]

Synonym: Cpar_1997

Alternate gene names: 193213633

Gene position: 2197075-2198391 (Clockwise)

Preceding gene: 193213630

Following gene: 193213634

Centisome position: 95.97

GC content: 59.68

Gene sequence:

>1317_bases
ATGAATAACGCAGATACCAGACAAAGGCTGGCCGATATTGAAAAGCAGATCGCGAGCCTTAAGGAGGAGCAGGCGACCGT
CAAGGCGCAGTGGGATTCTGAAAAAGAGCTGATTCACGGCTCACGCAAGCTCAAGGAGCAGATCGAAAACCTCAGGGTAG
AGGCCGAAAATTACGAACGAAGCGGCGATTACGGCAAGGTGGCCGAAATCCGCTACGGCAAGATTGCCGAGATAGAAAAA
CAGCTCGAAGAGAACAATCGCAAAATCGAAGCCCGGCAGGCCTCCGGCGACCTCATCATGAAAGAGGAGATCGACTCGGA
CGACATCGCCGACATCGTCTCCCGCTGGACGGGAATTCCGGTCAGCAAGATGCTTCAGTCCGAACGCCAGAAGCTGCTCG
GCATCGAGGCTGAACTGCACCGCCGCGTCGTCGGTCAAGACGAAGCTGTGCGCGCGGTCAGCGACGCGGTCAAGCGCTCG
CGGGCGGGTATGGGCGACGAAAAGCGGCCAATCGGCTCGTTCATCTTCCTCGGCCCCACCGGCGTCGGCAAGACCGAGCT
GGCCCGCACGCTGGCCGAATACCTGTTCGACGACGAGGACGCGCTGATCCGCATCGACATGAGCGAGTACATGGAGGCGC
ACACGGTGAGCCGCCTTGTCGGCGCGCCTCCCGGCTACGTCGGCTACGAAGAGGGCGGCCAGCTTACCGAAGCAGTGCGG
CGCAAACCTTTCTCGGTGGTGCTGCTCGACGAAATCGAAAAAGCGCACCCGGATGTGTTCAACATCCTGCTCCAGATTCT
CGATGACGGGCGGCTGACGGACAGCAAGGGCCGCACCGTGAACTTCAAGAACACCATCATCATCATGACCTCGAACATCG
GCGCCCAGCTCATCCAGAGCGAGATGGAGCGGATCGACGGCGAAGCTTCCGAAGCGGTGCTCGAAGGGTTGCAGGAAAAG
CTCTTCCAGCTGCTCAAGCAGCAGGTGCGACCCGAGTTCCTGAACCGCATCGACGAGGTGATTCTGTTCACGCCGCTGAC
CCAGGAGAACCTGCGCGAGATCGTGACCATCCAGTTCAACATCATCAAGGCGATGGCCGCCCGCCAGCGCATCACGCTCG
AAATCACCGACGAGGCGCTGATGTGGCTCGCCCGCACCGGCTTCGACCCTGCATTCGGCGCGCGTCCTCTCAAACGCGTC
ATGCAGCGGCAGATCACCAACCGACTGTCGGAGATGATCCTCGCCGGTCAGATTGGCGAAGACGACACCGTCGAGATCAG
CCTTGAAAACGACACCATCGTGATGAAGAAACGATAA

Upstream 100 bases:

>100_bases
CGGCGGTCAGAAGAACAGCCGAAGCGTCAGGATTCGCACATTGGAACAGCCAATTTAATCAATATTTTTTCTTTTCACTC
TCCATCAACGATAGCTGTTT

Downstream 100 bases:

>100_bases
CACCGAACCGCAAGCCGGACGGGGCTTTCCGAAGTTCCATCCGGCTTGTCACTTTCCCACCCACCATGAAAAAGAGCCTC
CTCGCGGCGCTGCTGCTTTT

Product: AAA ATPase-2 domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MNNADTRQRLADIEKQIASLKEEQATVKAQWDSEKELIHGSRKLKEQIENLRVEAENYERSGDYGKVAEIRYGKIAEIEK
QLEENNRKIEARQASGDLIMKEEIDSDDIADIVSRWTGIPVSKMLQSERQKLLGIEAELHRRVVGQDEAVRAVSDAVKRS
RAGMGDEKRPIGSFIFLGPTGVGKTELARTLAEYLFDDEDALIRIDMSEYMEAHTVSRLVGAPPGYVGYEEGGQLTEAVR
RKPFSVVLLDEIEKAHPDVFNILLQILDDGRLTDSKGRTVNFKNTIIIMTSNIGAQLIQSEMERIDGEASEAVLEGLQEK
LFQLLKQQVRPEFLNRIDEVILFTPLTQENLREIVTIQFNIIKAMAARQRITLEITDEALMWLARTGFDPAFGARPLKRV
MQRQITNRLSEMILAGQIGEDDTVEISLENDTIVMKKR

Sequences:

>Translated_438_residues
MNNADTRQRLADIEKQIASLKEEQATVKAQWDSEKELIHGSRKLKEQIENLRVEAENYERSGDYGKVAEIRYGKIAEIEK
QLEENNRKIEARQASGDLIMKEEIDSDDIADIVSRWTGIPVSKMLQSERQKLLGIEAELHRRVVGQDEAVRAVSDAVKRS
RAGMGDEKRPIGSFIFLGPTGVGKTELARTLAEYLFDDEDALIRIDMSEYMEAHTVSRLVGAPPGYVGYEEGGQLTEAVR
RKPFSVVLLDEIEKAHPDVFNILLQILDDGRLTDSKGRTVNFKNTIIIMTSNIGAQLIQSEMERIDGEASEAVLEGLQEK
LFQLLKQQVRPEFLNRIDEVILFTPLTQENLREIVTIQFNIIKAMAARQRITLEITDEALMWLARTGFDPAFGARPLKRV
MQRQITNRLSEMILAGQIGEDDTVEISLENDTIVMKKR
>Mature_438_residues
MNNADTRQRLADIEKQIASLKEEQATVKAQWDSEKELIHGSRKLKEQIENLRVEAENYERSGDYGKVAEIRYGKIAEIEK
QLEENNRKIEARQASGDLIMKEEIDSDDIADIVSRWTGIPVSKMLQSERQKLLGIEAELHRRVVGQDEAVRAVSDAVKRS
RAGMGDEKRPIGSFIFLGPTGVGKTELARTLAEYLFDDEDALIRIDMSEYMEAHTVSRLVGAPPGYVGYEEGGQLTEAVR
RKPFSVVLLDEIEKAHPDVFNILLQILDDGRLTDSKGRTVNFKNTIIIMTSNIGAQLIQSEMERIDGEASEAVLEGLQEK
LFQLLKQQVRPEFLNRIDEVILFTPLTQENLREIVTIQFNIIKAMAARQRITLEITDEALMWLARTGFDPAFGARPLKRV
MQRQITNRLSEMILAGQIGEDDTVEISLENDTIVMKKR

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=319, Percent_Identity=36.6771159874608, Blast_Score=210, Evalue=2e-54,
Organism=Escherichia coli, GI1788943, Length=427, Percent_Identity=50.3512880562061, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI1787109, Length=331, Percent_Identity=41.9939577039275, Blast_Score=273, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6320464, Length=426, Percent_Identity=45.7746478873239, Blast_Score=392, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6323002, Length=434, Percent_Identity=40.3225806451613, Blast_Score=324, Evalue=1e-89,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR001270
- InterPro:   IPR019489 [H]

Pfam domain/function: PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 49652; Mature: 49652

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNADTRQRLADIEKQIASLKEEQATVKAQWDSEKELIHGSRKLKEQIENLRVEAENYER
CCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHEECHHHCCC
SGDYGKVAEIRYGKIAEIEKQLEENNRKIEARQASGDLIMKEEIDSDDIADIVSRWTGIP
CCCCCCCHHCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHCCCC
VSKMLQSERQKLLGIEAELHRRVVGQDEAVRAVSDAVKRSRAGMGDEKRPIGSFIFLGPT
HHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCC
GVGKTELARTLAEYLFDDEDALIRIDMSEYMEAHTVSRLVGAPPGYVGYEEGGQLTEAVR
CCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
RKPFSVVLLDEIEKAHPDVFNILLQILDDGRLTDSKGRTVNFKNTIIIMTSNIGAQLIQS
CCCCEEEEHHHHHHHCCHHHHHHHHHHCCCCEECCCCCEEEECCEEEEEECCHHHHHHHH
EMERIDGEASEAVLEGLQEKLFQLLKQQVRPEFLNRIDEVILFTPLTQENLREIVTIQFN
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHEEEECCCCHHHHHHHHHHHHH
IIKAMAARQRITLEITDEALMWLARTGFDPAFGARPLKRVMQRQITNRLSEMILAGQIGE
HHHHHHHCCEEEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
DDTVEISLENDTIVMKKR
CCEEEEEECCCEEEEECC
>Mature Secondary Structure
MNNADTRQRLADIEKQIASLKEEQATVKAQWDSEKELIHGSRKLKEQIENLRVEAENYER
CCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHEECHHHCCC
SGDYGKVAEIRYGKIAEIEKQLEENNRKIEARQASGDLIMKEEIDSDDIADIVSRWTGIP
CCCCCCCHHCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHCCCC
VSKMLQSERQKLLGIEAELHRRVVGQDEAVRAVSDAVKRSRAGMGDEKRPIGSFIFLGPT
HHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCC
GVGKTELARTLAEYLFDDEDALIRIDMSEYMEAHTVSRLVGAPPGYVGYEEGGQLTEAVR
CCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
RKPFSVVLLDEIEKAHPDVFNILLQILDDGRLTDSKGRTVNFKNTIIIMTSNIGAQLIQS
CCCCEEEEHHHHHHHCCHHHHHHHHHHCCCCEECCCCCEEEECCEEEEEECCHHHHHHHH
EMERIDGEASEAVLEGLQEKLFQLLKQQVRPEFLNRIDEVILFTPLTQENLREIVTIQFN
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHEEEECCCCHHHHHHHHHHHHH
IIKAMAARQRITLEITDEALMWLARTGFDPAFGARPLKRVMQRQITNRLSEMILAGQIGE
HHHHHHHCCEEEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
DDTVEISLENDTIVMKKR
CCEEEEEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]