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The map label for this gene is proS

Identifier: 193213223

GI number: 193213223

Start: 1726859

End: 1728304

Strand: Direct

Name: proS

Synonym: Cpar_1578

Alternate gene names: 193213223

Gene position: 1726859-1728304 (Clockwise)

Preceding gene: 193213222

Following gene: 193213225

Centisome position: 75.43

GC content: 59.89

Gene sequence:

>1446_bases
GTGGCTGATAAAATCACTTCGAGGCAGCAGGACTATTCTCAATGGTATATCGACCTGGTCAGGTCGGCCAAACTTGCCGA
CTATTCGGACGTGCGTGGCTGCATGGTCATCCGACCGAACGGCTACGCCATCTGGGAGAAGATGCAGGCCGCGCTCGACG
GCATGTTCAAGGAAACCGGCCATGTGAACGCCTATTTCCCGATGTTCATCCCCGAAAGCTTCATCGCCAAAGAGGCCGAG
CACATCGAAGGATTCGCGCCGGAGTGCGCCGTGGTGACGCATGGCGGCGGCGAGGAGCTTGCCGAAAAACTCTACGTCCG
CCCGACTTCGGAGACCATCATCTGGTCGTCGTATAAAAAATGGATCCAATCCTACCGCGACCTGCCACTCCTTATCAACC
AGTGGGCCAACGTCGTGCGCTGGGAGATGCGCACCCGCCTGTTCCTGCGGACGACCGAGTTCCTCTGGCAGGAGGGGCAC
ACGGCCCACGCCGATCCGGAAGAGGCGCAGGAAGAGGTGATCCGCATGATTAACGTGTACCGCACCTTCGCCGAAGAGTA
CATGGCGATGCCGGTGATCGTCGGCAAAAAGAGCGAGAGCGAGAAGTTCGCCGGTGCGGACGCGACCTACTGCATCGAAG
CGATGATGCAGGACGGCAAGGCGTTGCAGGCAGGCACCTCGCACAACCTCGGCCAGAACTTCGCCAAGGCGTTCGACTGC
CAGTTCCAGACCAAAGAGGGCGTGCTGGACTACGTCTGGGCCACGAGCTGGGGCGTTTCGACTCGGCTGATCGGCGCGCT
CATCATGGCGCACTCCGACGACAAGGGCCTCGTGCTGCCGCCGAAACTCGCGTCGCGCCAGGTGGTGATTATTCCGATCC
TCAAGGGCGACAAGGAGGAGGTTCGCGCGCACGCGCGCTTTATCGCCAAGGCGCTCAACAAGCACGGCATCTCGACCTTC
GTCGATGACAGCGAGAACAACTCGCCCGGCTGGAAATTCGCCGAGTACGAGCTGCAGGGCATTCCGGTCAGGATCGAACT
CGGCCCGCGCGACATCGCAGAGGGCAAATGCATCGTCGCCCGCCGCGACACCTTCGAAAAAACCGAAGTGCTGCTCGACG
CAGAGATGACCGAAAACATCGAGGAAATCCTGAACAACATCCAGCAGAACCTCTACGAACGCGCCCTGCAGTTCAGGCAG
GACAACACGGTTGAAGCGACAACCTGGGAGGAGTTCAAAGCAGGCGTGGAGAAAGGATTCGTCATCGCCCACTGGGACGG
CACGGATGAAACCGAAGCGCTCATCAAGGAGGAAACCAAAGCCACGATCCGTGTGATGCCAACTGATGAAGAGTACCGCA
AACAGTACAACATGGACGAGCCGGGCACCTGCATCCGCAGCGGCAACCCCGCCACGCAGAAGGTGGTGTTCGCCAAGGCG
TACTGA

Upstream 100 bases:

>100_bases
TTCCACCTCGCATTATTACTTTACAGGTTTACCGTCATAACCTTATGGCGCCCGGCGACTCCGGGCTCATTTCCCGTCAA
ACGAAAACACAAGCATTACC

Downstream 100 bases:

>100_bases
TCAGTTGAAAATGGATAGTTAGTAATTATGGGGCGATCGGTTGATCGCCCTTTTTTTGTCCACCGCGCCCACAATTCCTC
ACCACAAAAAAATGCCGGGA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MADKITSRQQDYSQWYIDLVRSAKLADYSDVRGCMVIRPNGYAIWEKMQAALDGMFKETGHVNAYFPMFIPESFIAKEAE
HIEGFAPECAVVTHGGGEELAEKLYVRPTSETIIWSSYKKWIQSYRDLPLLINQWANVVRWEMRTRLFLRTTEFLWQEGH
TAHADPEEAQEEVIRMINVYRTFAEEYMAMPVIVGKKSESEKFAGADATYCIEAMMQDGKALQAGTSHNLGQNFAKAFDC
QFQTKEGVLDYVWATSWGVSTRLIGALIMAHSDDKGLVLPPKLASRQVVIIPILKGDKEEVRAHARFIAKALNKHGISTF
VDDSENNSPGWKFAEYELQGIPVRIELGPRDIAEGKCIVARRDTFEKTEVLLDAEMTENIEEILNNIQQNLYERALQFRQ
DNTVEATTWEEFKAGVEKGFVIAHWDGTDETEALIKEETKATIRVMPTDEEYRKQYNMDEPGTCIRSGNPATQKVVFAKA
Y

Sequences:

>Translated_481_residues
MADKITSRQQDYSQWYIDLVRSAKLADYSDVRGCMVIRPNGYAIWEKMQAALDGMFKETGHVNAYFPMFIPESFIAKEAE
HIEGFAPECAVVTHGGGEELAEKLYVRPTSETIIWSSYKKWIQSYRDLPLLINQWANVVRWEMRTRLFLRTTEFLWQEGH
TAHADPEEAQEEVIRMINVYRTFAEEYMAMPVIVGKKSESEKFAGADATYCIEAMMQDGKALQAGTSHNLGQNFAKAFDC
QFQTKEGVLDYVWATSWGVSTRLIGALIMAHSDDKGLVLPPKLASRQVVIIPILKGDKEEVRAHARFIAKALNKHGISTF
VDDSENNSPGWKFAEYELQGIPVRIELGPRDIAEGKCIVARRDTFEKTEVLLDAEMTENIEEILNNIQQNLYERALQFRQ
DNTVEATTWEEFKAGVEKGFVIAHWDGTDETEALIKEETKATIRVMPTDEEYRKQYNMDEPGTCIRSGNPATQKVVFAKA
Y
>Mature_480_residues
ADKITSRQQDYSQWYIDLVRSAKLADYSDVRGCMVIRPNGYAIWEKMQAALDGMFKETGHVNAYFPMFIPESFIAKEAEH
IEGFAPECAVVTHGGGEELAEKLYVRPTSETIIWSSYKKWIQSYRDLPLLINQWANVVRWEMRTRLFLRTTEFLWQEGHT
AHADPEEAQEEVIRMINVYRTFAEEYMAMPVIVGKKSESEKFAGADATYCIEAMMQDGKALQAGTSHNLGQNFAKAFDCQ
FQTKEGVLDYVWATSWGVSTRLIGALIMAHSDDKGLVLPPKLASRQVVIIPILKGDKEEVRAHARFIAKALNKHGISTFV
DDSENNSPGWKFAEYELQGIPVRIELGPRDIAEGKCIVARRDTFEKTEVLLDAEMTENIEEILNNIQQNLYERALQFRQD
NTVEATTWEEFKAGVEKGFVIAHWDGTDETEALIKEETKATIRVMPTDEEYRKQYNMDEPGTCIRSGNPATQKVVFAKAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=494, Percent_Identity=39.8785425101215, Blast_Score=377, Evalue=1e-104,
Organism=Escherichia coli, GI1786392, Length=134, Percent_Identity=29.8507462686567, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71991342, Length=446, Percent_Identity=42.152466367713, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71991346, Length=357, Percent_Identity=46.218487394958, Blast_Score=355, Evalue=3e-98,
Organism=Saccharomyces cerevisiae, GI6321808, Length=441, Percent_Identity=45.3514739229025, Blast_Score=398, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24649466, Length=443, Percent_Identity=40.6320541760722, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24649468, Length=443, Percent_Identity=40.6320541760722, Blast_Score=357, Evalue=1e-98,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_CHLP8 (B3QPX3)

Other databases:

- EMBL:   CP001099
- RefSeq:   YP_001999176.1
- GeneID:   6420520
- GenomeReviews:   CP001099_GR
- KEGG:   cpc:Cpar_1578
- HOGENOM:   HBG334108
- OMA:   PGWKFNE
- ProtClustDB:   PRK08661
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- PRINTS:   PR01046
- SMART:   SM00946
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 54823; Mature: 54691

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKITSRQQDYSQWYIDLVRSAKLADYSDVRGCMVIRPNGYAIWEKMQAALDGMFKETG
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCC
HVNAYFPMFIPESFIAKEAEHIEGFAPECAVVTHGGGEELAEKLYVRPTSETIIWSSYKK
CCEEEEHHHCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCCEEEHHHHHH
WIQSYRDLPLLINQWANVVRWEMRTRLFLRTTEFLWQEGHTAHADPEEAQEEVIRMINVY
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
RTFAEEYMAMPVIVGKKSESEKFAGADATYCIEAMMQDGKALQAGTSHNLGQNFAKAFDC
HHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCC
QFQTKEGVLDYVWATSWGVSTRLIGALIMAHSDDKGLVLPPKLASRQVVIIPILKGDKEE
CCCCCCCCCHHHHHCCCCHHHHHHHHHHHEECCCCCCEECCCCCCCCEEEEEECCCCHHH
VRAHARFIAKALNKHGISTFVDDSENNSPGWKFAEYELQGIPVRIELGPRDIAEGKCIVA
HHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEECCEEEEEEECCCCCCCCCEEEE
RRDTFEKTEVLLDAEMTENIEEILNNIQQNLYERALQFRQDNTVEATTWEEFKAGVEKGF
ECCCCCHHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCE
VIAHWDGTDETEALIKEETKATIRVMPTDEEYRKQYNMDEPGTCIRSGNPATQKVVFAKA
EEEEECCCCHHHHHHHHCCCCEEEEECCCHHHHHHCCCCCCCCEEECCCCCHHEEEEEEC
Y
C
>Mature Secondary Structure 
ADKITSRQQDYSQWYIDLVRSAKLADYSDVRGCMVIRPNGYAIWEKMQAALDGMFKETG
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCC
HVNAYFPMFIPESFIAKEAEHIEGFAPECAVVTHGGGEELAEKLYVRPTSETIIWSSYKK
CCEEEEHHHCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCCEEEHHHHHH
WIQSYRDLPLLINQWANVVRWEMRTRLFLRTTEFLWQEGHTAHADPEEAQEEVIRMINVY
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
RTFAEEYMAMPVIVGKKSESEKFAGADATYCIEAMMQDGKALQAGTSHNLGQNFAKAFDC
HHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCC
QFQTKEGVLDYVWATSWGVSTRLIGALIMAHSDDKGLVLPPKLASRQVVIIPILKGDKEE
CCCCCCCCCHHHHHCCCCHHHHHHHHHHHEECCCCCCEECCCCCCCCEEEEEECCCCHHH
VRAHARFIAKALNKHGISTFVDDSENNSPGWKFAEYELQGIPVRIELGPRDIAEGKCIVA
HHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEECCEEEEEEECCCCCCCCCEEEE
RRDTFEKTEVLLDAEMTENIEEILNNIQQNLYERALQFRQDNTVEATTWEEFKAGVEKGF
ECCCCCHHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCE
VIAHWDGTDETEALIKEETKATIRVMPTDEEYRKQYNMDEPGTCIRSGNPATQKVVFAKA
EEEEECCCCHHHHHHHHCCCCEEEEECCCHHHHHHCCCCCCCCEEECCCCCHHEEEEEEC
Y
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA