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The map label for this gene is rlmI [H]

Identifier: 193212806

GI number: 193212806

Start: 1269277

End: 1270461

Strand: Direct

Name: rlmI [H]

Synonym: Cpar_1153

Alternate gene names: 193212806

Gene position: 1269277-1270461 (Clockwise)

Preceding gene: 193212804

Following gene: 193212809

Centisome position: 55.45

GC content: 56.2

Gene sequence:

>1185_bases
ATGCACGCACTTTACCTCAAACCGAAAGAGCATCGGCGGCTGCTTGGCGGCCACCTGTGGGTTTTCAGCAACGAACTCAA
AGAGGTGCCGCGTGACATCGCTGCTGGAGAAACCGTCCAGCTCTTCACTAACGACGGGCGGCTACTCGGCACCGGCTTTT
TCAATCCGCACTCGCTGATCGCTTTCCGCCTGCTGACCAGAGACGAAACCCAGCCTGATCGGGAATTTTTCCGCAATAAA
ATCCGAGAAGCGCTCAAACTGCGCGAAAAAATCTATCCGGAAAGCGAAACCAACGCCTGGCGGCTGGTGCACGGGGAGTC
GGACGGCTTGCCGGGCCTGGTGATTGACCGGTTTGACAAAGCTTTCGTCCTGCAATCCTTTTCGGCTGGTATGGAAAACC
ACCTGCCGATCATCACCGAAGTACTGCGAGAGCTGTTCGACCCAAAAGCGATTGTGCTGCGCAACGAATCGCCACTCAGG
GAACTCGAAGGCTTGCCGCTCTACAAAGAGATGCTTTTGGGTGATGAAAACGATGCCTGGCAGGTCATCAATGACGATGG
CATCAGCTACCGGGTCAACATTCTCGAAGGACAGAAGACGGGCTTTTTCCTCGACCAGCGCGAAAACCGCCGCCACATCC
GGAAATACGCCGCGGATGCCGACGTACTCGACGTCTATACCAACGACGGAGGCTTTGCGCTCAATGCCATGCACGCCGGA
GCGCGATCAACGACGATGGTCGATATATCGCAGGATGCACTTCAACGCGCCGAGCAGAACGCCCGCATGAATGGCCTGGA
GAAGTTCTCGATCGTGGCGGATGACGCATTTGCAACGCTTGGCCGACTCAGGCAGGAAAACCGCTCGTACGATCTGGTGA
TTCTCGACCCGCCAAGCTTCACGAAGAGCCGCAAAACCGTTCCGACCGCCCTGAAAGCGTATACCAAGCTTAACCGCCTT
GGTCTTCAGCTCGTCAAAAATGAAGGATTTCTGGCCACAGCCTCATGCTCACACCACGTTTCCGAAGAGGATTTTCTGGC
CGCTGTCCAGCTCGGGGCACTACACGCAGGCAAGCACCTCAGACTCATCTCCCGCAATGCGCAGCCGCCGGATCACCCGA
TACTGCTCTCCATGCCCGAAACCAGCTACCTCAAGTTCGCCTGCTTTTACGTAACGAACCTGTAA

Upstream 100 bases:

>100_bases
TGCCGGGAATGTCAGTTCCCGCATGAGGTGGATTGTTATGAAATGTTATTTTAATCAAATCCTGTCTCTATCAAAACACG
TCTTCCGGAGTTTTCGATCC

Downstream 100 bases:

>100_bases
TCCCGAAAAGTTGAGCTGACTGTTCATGCTCGTCACCCGATATAGATCAGGTGACGAGAGGCTCAGGCTCCGGAGAAAAC
GCAGCACTTTACGACATAAC

Product: PUA domain-containing protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MHALYLKPKEHRRLLGGHLWVFSNELKEVPRDIAAGETVQLFTNDGRLLGTGFFNPHSLIAFRLLTRDETQPDREFFRNK
IREALKLREKIYPESETNAWRLVHGESDGLPGLVIDRFDKAFVLQSFSAGMENHLPIITEVLRELFDPKAIVLRNESPLR
ELEGLPLYKEMLLGDENDAWQVINDDGISYRVNILEGQKTGFFLDQRENRRHIRKYAADADVLDVYTNDGGFALNAMHAG
ARSTTMVDISQDALQRAEQNARMNGLEKFSIVADDAFATLGRLRQENRSYDLVILDPPSFTKSRKTVPTALKAYTKLNRL
GLQLVKNEGFLATASCSHHVSEEDFLAAVQLGALHAGKHLRLISRNAQPPDHPILLSMPETSYLKFACFYVTNL

Sequences:

>Translated_394_residues
MHALYLKPKEHRRLLGGHLWVFSNELKEVPRDIAAGETVQLFTNDGRLLGTGFFNPHSLIAFRLLTRDETQPDREFFRNK
IREALKLREKIYPESETNAWRLVHGESDGLPGLVIDRFDKAFVLQSFSAGMENHLPIITEVLRELFDPKAIVLRNESPLR
ELEGLPLYKEMLLGDENDAWQVINDDGISYRVNILEGQKTGFFLDQRENRRHIRKYAADADVLDVYTNDGGFALNAMHAG
ARSTTMVDISQDALQRAEQNARMNGLEKFSIVADDAFATLGRLRQENRSYDLVILDPPSFTKSRKTVPTALKAYTKLNRL
GLQLVKNEGFLATASCSHHVSEEDFLAAVQLGALHAGKHLRLISRNAQPPDHPILLSMPETSYLKFACFYVTNL
>Mature_394_residues
MHALYLKPKEHRRLLGGHLWVFSNELKEVPRDIAAGETVQLFTNDGRLLGTGFFNPHSLIAFRLLTRDETQPDREFFRNK
IREALKLREKIYPESETNAWRLVHGESDGLPGLVIDRFDKAFVLQSFSAGMENHLPIITEVLRELFDPKAIVLRNESPLR
ELEGLPLYKEMLLGDENDAWQVINDDGISYRVNILEGQKTGFFLDQRENRRHIRKYAADADVLDVYTNDGGFALNAMHAG
ARSTTMVDISQDALQRAEQNARMNGLEKFSIVADDAFATLGRLRQENRSYDLVILDPPSFTKSRKTVPTALKAYTKLNRL
GLQLVKNEGFLATASCSHHVSEEDFLAAVQLGALHAGKHLRLISRNAQPPDHPILLSMPETSYLKFACFYVTNL

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=389, Percent_Identity=34.1902313624679, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1787181, Length=257, Percent_Identity=28.7937743190661, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF01472 PUA [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 44701; Mature: 44701

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHALYLKPKEHRRLLGGHLWVFSNELKEVPRDIAAGETVQLFTNDGRLLGTGFFNPHSLI
CCEEECCCHHHHHHHCCEEEEEEHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCHHHHH
AFRLLTRDETQPDREFFRNKIREALKLREKIYPESETNAWRLVHGESDGLPGLVIDRFDK
HEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHCCH
AFVLQSFSAGMENHLPIITEVLRELFDPKAIVLRNESPLRELEGLPLYKEMLLGDENDAW
HHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCHHHHHHCCCCCCCE
QVINDDGISYRVNILEGQKTGFFLDQRENRRHIRKYAADADVLDVYTNDGGFALNAMHAG
EEEECCCCEEEEEEEECCCCCEEECCCCCHHHHHHHHCCCCEEEEEECCCCEEEEEECCC
ARSTTMVDISQDALQRAEQNARMNGLEKFSIVADDAFATLGRLRQENRSYDLVILDPPSF
CCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHCCCCEEEEEECCCCC
TKSRKTVPTALKAYTKLNRLGLQLVKNEGFLATASCSHHVSEEDFLAAVQLGALHAGKHL
CCCCCHHHHHHHHHHHHHHHHHHEEECCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEE
RLISRNAQPPDHPILLSMPETSYLKFACFYVTNL
EEEECCCCCCCCCEEEECCCCCHHEEEEEEEECC
>Mature Secondary Structure
MHALYLKPKEHRRLLGGHLWVFSNELKEVPRDIAAGETVQLFTNDGRLLGTGFFNPHSLI
CCEEECCCHHHHHHHCCEEEEEEHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCHHHHH
AFRLLTRDETQPDREFFRNKIREALKLREKIYPESETNAWRLVHGESDGLPGLVIDRFDK
HEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHCCH
AFVLQSFSAGMENHLPIITEVLRELFDPKAIVLRNESPLRELEGLPLYKEMLLGDENDAW
HHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCHHHHHHCCCCCCCE
QVINDDGISYRVNILEGQKTGFFLDQRENRRHIRKYAADADVLDVYTNDGGFALNAMHAG
EEEECCCCEEEEEEEECCCCCEEECCCCCHHHHHHHHCCCCEEEEEECCCCEEEEEECCC
ARSTTMVDISQDALQRAEQNARMNGLEKFSIVADDAFATLGRLRQENRSYDLVILDPPSF
CCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHCCCCEEEEEECCCCC
TKSRKTVPTALKAYTKLNRLGLQLVKNEGFLATASCSHHVSEEDFLAAVQLGALHAGKHL
CCCCCHHHHHHHHHHHHHHHHHHEEECCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEE
RLISRNAQPPDHPILLSMPETSYLKFACFYVTNL
EEEECCCCCCCCCEEEECCCCCHHEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA