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The map label for this gene is mscL

Identifier: 193212607

GI number: 193212607

Start: 1046919

End: 1047374

Strand: Direct

Name: mscL

Synonym: Cpar_0951

Alternate gene names: 193212607

Gene position: 1046919-1047374 (Clockwise)

Preceding gene: 193212594

Following gene: 193212608

Centisome position: 45.73

GC content: 53.73

Gene sequence:

>456_bases
ATGCTGAAAGAGTTCAAGGAATTCGCCCTGAAGGGTAACGTTGTCGATATGGCTGTCGGCATCATTATTGGTGGTGCTTT
TACTGGCATCGTTAAATCACTGGTCGGCGATGTTCTGACTCCGCCGCTCGGGCTTCTGCTCAACGGAGTCGATTTTACCA
ATCTGTTTGTTGTGCTCAAGGAGGGGGCTACTCCTGGGCCGTACCTTGCGCTTGAGCAGGCGCAGAGCGCTGGAGCGGTA
ACGCTTAATTATGGCCTGTTTATCAATGCGTTCATAAGTTTTGTGATTATGGCTGTCGCCGTGTTTTTCCTGGTTCGCGG
CATCAATAGACTGCGTAAGATGACCGAAAAACCCCCGGAGCCAGCCGCCGCTCCCGATACCAAGGAGTGCCCGTTCTGCT
TCTCGGCCATCCCTGTCAAGGCCGTGCGTTGCCCGAACTGCACGTCGCAGTTGTAA

Upstream 100 bases:

>100_bases
AAATACACTAATGTCCAATTAAGGGGGTTTAATTTCTTGATATTCCCTCGTTGTCCCCCATGACACCAGAGTACTTCCTC
AAATTTCAATAACCGGAACC

Downstream 100 bases:

>100_bases
GCGCGTGAATTCATGGGGAGTTTCCATAACAATTTGCAAGTTCCCCTATGGAGATCAACGGCTTTTTGTTTATGATTGGA
GTTGTTTTCATAACCTATAG

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MLKEFKEFALKGNVVDMAVGIIIGGAFTGIVKSLVGDVLTPPLGLLLNGVDFTNLFVVLKEGATPGPYLALEQAQSAGAV
TLNYGLFINAFISFVIMAVAVFFLVRGINRLRKMTEKPPEPAAAPDTKECPFCFSAIPVKAVRCPNCTSQL

Sequences:

>Translated_151_residues
MLKEFKEFALKGNVVDMAVGIIIGGAFTGIVKSLVGDVLTPPLGLLLNGVDFTNLFVVLKEGATPGPYLALEQAQSAGAV
TLNYGLFINAFISFVIMAVAVFFLVRGINRLRKMTEKPPEPAAAPDTKECPFCFSAIPVKAVRCPNCTSQL
>Mature_151_residues
MLKEFKEFALKGNVVDMAVGIIIGGAFTGIVKSLVGDVLTPPLGLLLNGVDFTNLFVVLKEGATPGPYLALEQAQSAGAV
TLNYGLFINAFISFVIMAVAVFFLVRGINRLRKMTEKPPEPAAAPDTKECPFCFSAIPVKAVRCPNCTSQL

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=130, Percent_Identity=46.9230769230769, Blast_Score=110, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_CHLP8 (B3QN57)

Other databases:

- EMBL:   CP001099
- RefSeq:   YP_001998560.1
- GeneID:   6419881
- GenomeReviews:   CP001099_GR
- KEGG:   cpc:Cpar_0951
- HOGENOM:   HBG727604
- OMA:   IKATRCP
- ProtClustDB:   PRK00567
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 16093; Mature: 16093

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1b8380d4)-; HASH(0x1bb3a138)-;

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEFKEFALKGNVVDMAVGIIIGGAFTGIVKSLVGDVLTPPLGLLLNGVDFTNLFVVLK
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHEE
EGATPGPYLALEQAQSAGAVTLNYGLFINAFISFVIMAVAVFFLVRGINRLRKMTEKPPE
CCCCCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PAAAPDTKECPFCFSAIPVKAVRCPNCTSQL
CCCCCCCCCCCCHHHHCCHHHEECCCCCCCH
>Mature Secondary Structure
MLKEFKEFALKGNVVDMAVGIIIGGAFTGIVKSLVGDVLTPPLGLLLNGVDFTNLFVVLK
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHEE
EGATPGPYLALEQAQSAGAVTLNYGLFINAFISFVIMAVAVFFLVRGINRLRKMTEKPPE
CCCCCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PAAAPDTKECPFCFSAIPVKAVRCPNCTSQL
CCCCCCCCCCCCHHHHCCHHHEECCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA