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The map label for this gene is lpd

Identifier: 193212390

GI number: 193212390

Start: 775858

End: 777267

Strand: Direct

Name: lpd

Synonym: Cpar_0724

Alternate gene names: 193212390

Gene position: 775858-777267 (Clockwise)

Preceding gene: 193212386

Following gene: 193212391

Centisome position: 33.89

GC content: 62.27

Gene sequence:

>1410_bases
ATGCAGCAGTCAGAGAGTTCGTCAGCGCAGTTCGATGTGGCTGTCATCGGTTCCGGCCCCGGTGGTTACGAAGCGGCGCT
TCATGCAGCCCGTCACGGCATGAAGGTGTGTCTTGTTGAAAAGGCTTCGCTTGGCGGGGTTTGCGTGAACTGGGGCTGTA
TTCCGACCAAGGCGCTGCTCCGGAGTGCCGAAGTGTACGATCTGGCGAAGAATCCGTCGGAGTTCGGCGTGAACGTCAGC
GAGTTGTCGTTTGATCTCGCGCAGGCTGTCAAGCGCAGCCGGAAGGTGTCGCTGAAAAGTTCCAAAGGTGTCGAATTCAT
GCTGAAAAAGGCAAAGGTCGAGGTGTGGAGAGGCGAAGCGGTGCTGACTGGTAGTAAAGGTGTGAAGGTGACCGCGGAGG
ATGGTTCGGAGCGTTCGCTCGAAGCGGCGAACATCATCGTCGCGACCGGCGCGCAGCCGCGCGTAATTCCGGGGCTTGAA
CCGGACGGCAAGAAGATCATCACGAGCCGAGAGGCGCTGATTCTGAAAGATGTTCCGGAGTCGATGATCGTGGTCGGCGG
CGGGGCGATCGGCGTCGAGATGGCCTGGTTCTACGCCAAGGCGGGGGCGAAAGTGACCATCGTCGAACTGATGCCGCGCT
TGTTGCCAGCTGAGGAGGCGGAGGTTTCCGAAGCGCTGAAACGCTCGTTCGAGAAGGTGGATATTACCGTGCAGTGCGGT
GCGAAGCTCGGCAATGTCGCCATCAGCGAGTTCGGTGTGAATGCCGATTTGCTCGCGGAGGGGAAGGAGCCTCAGAAAAT
CGAGGCTTCGTGTATGCTCGTGGCGGTGGGCGTGACTGGCGTCATCGATGGCCTCGGCCTCGATGCGGCGGGCATCGAGA
CGGAGCGCGGATTCATTCGCACCGACGAACTGTGCCGCACCTCCGCATCCGGCATTTACGCTATCGGCGACGTGCGTGGC
GGAATGCTGCTGGCGCATAAGGCCTCGGCGGAGGCGGCGATTGCGGTCGAGGCAATCGCCGGAAAGTTGCCCGAGCCGCT
ATCCGAGCCGTTGATTCCTCGATGTGTCTATGCCCAGCCCTCGGTGGCATCGGTCGGCCTGACCGAAGAGGCTGCGATTG
CGGCGGGTTACAAGGTGCTGGTCGGACGTTCGCAGTTTGCTGCATCCGGTAAAGCCAACGCCTACGGCCAGCTCGAAGGC
TTCGTCAAGCTGGTCTTCAACGCCGAAACCGGCAAGATGCTCGGCGGCCACCTCATCGGCCACGACGCGGTCGAGCTGAT
CGGCGAATTGGGGCTGGCGTGCCGCTACGGCGTGACCGCCGAAGGGCTGGTCGGCACGGTGCACGCGCACCCGACGCTCT
CGGAGACGGTTCGCGAGGCCGCGTTCGCCGCGCTGCAAAGCAAGGGGTGA

Upstream 100 bases:

>100_bases
CTCGTGAATGTTATTATAGTCAAAGAATCATCCTTTGCAACTTTGATCGTTTGTCTTCGCTGATTCGAGGCGTGGTTGCG
GTCAATAAATTCAGAATTCC

Downstream 100 bases:

>100_bases
CCGGGTGAGCGGGTTTTCGATCAGTCGGATCAGACCAATCGGTCTGATCCGACTGATTTTCCTTCTCCCAAAATCGCTCT
TTAACCGCCTGTTTTCGCCA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MQQSESSSAQFDVAVIGSGPGGYEAALHAARHGMKVCLVEKASLGGVCVNWGCIPTKALLRSAEVYDLAKNPSEFGVNVS
ELSFDLAQAVKRSRKVSLKSSKGVEFMLKKAKVEVWRGEAVLTGSKGVKVTAEDGSERSLEAANIIVATGAQPRVIPGLE
PDGKKIITSREALILKDVPESMIVVGGGAIGVEMAWFYAKAGAKVTIVELMPRLLPAEEAEVSEALKRSFEKVDITVQCG
AKLGNVAISEFGVNADLLAEGKEPQKIEASCMLVAVGVTGVIDGLGLDAAGIETERGFIRTDELCRTSASGIYAIGDVRG
GMLLAHKASAEAAIAVEAIAGKLPEPLSEPLIPRCVYAQPSVASVGLTEEAAIAAGYKVLVGRSQFAASGKANAYGQLEG
FVKLVFNAETGKMLGGHLIGHDAVELIGELGLACRYGVTAEGLVGTVHAHPTLSETVREAAFAALQSKG

Sequences:

>Translated_469_residues
MQQSESSSAQFDVAVIGSGPGGYEAALHAARHGMKVCLVEKASLGGVCVNWGCIPTKALLRSAEVYDLAKNPSEFGVNVS
ELSFDLAQAVKRSRKVSLKSSKGVEFMLKKAKVEVWRGEAVLTGSKGVKVTAEDGSERSLEAANIIVATGAQPRVIPGLE
PDGKKIITSREALILKDVPESMIVVGGGAIGVEMAWFYAKAGAKVTIVELMPRLLPAEEAEVSEALKRSFEKVDITVQCG
AKLGNVAISEFGVNADLLAEGKEPQKIEASCMLVAVGVTGVIDGLGLDAAGIETERGFIRTDELCRTSASGIYAIGDVRG
GMLLAHKASAEAAIAVEAIAGKLPEPLSEPLIPRCVYAQPSVASVGLTEEAAIAAGYKVLVGRSQFAASGKANAYGQLEG
FVKLVFNAETGKMLGGHLIGHDAVELIGELGLACRYGVTAEGLVGTVHAHPTLSETVREAAFAALQSKG
>Mature_469_residues
MQQSESSSAQFDVAVIGSGPGGYEAALHAARHGMKVCLVEKASLGGVCVNWGCIPTKALLRSAEVYDLAKNPSEFGVNVS
ELSFDLAQAVKRSRKVSLKSSKGVEFMLKKAKVEVWRGEAVLTGSKGVKVTAEDGSERSLEAANIIVATGAQPRVIPGLE
PDGKKIITSREALILKDVPESMIVVGGGAIGVEMAWFYAKAGAKVTIVELMPRLLPAEEAEVSEALKRSFEKVDITVQCG
AKLGNVAISEFGVNADLLAEGKEPQKIEASCMLVAVGVTGVIDGLGLDAAGIETERGFIRTDELCRTSASGIYAIGDVRG
GMLLAHKASAEAAIAVEAIAGKLPEPLSEPLIPRCVYAQPSVASVGLTEEAAIAAGYKVLVGRSQFAASGKANAYGQLEG
FVKLVFNAETGKMLGGHLIGHDAVELIGELGLACRYGVTAEGLVGTVHAHPTLSETVREAAFAALQSKG

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family

Homologues:

Organism=Homo sapiens, GI91199540, Length=460, Percent_Identity=36.5217391304348, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI50301238, Length=458, Percent_Identity=28.6026200873362, Blast_Score=142, Evalue=9e-34,
Organism=Homo sapiens, GI22035672, Length=466, Percent_Identity=28.1115879828326, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI291045266, Length=433, Percent_Identity=24.2494226327945, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI148277071, Length=469, Percent_Identity=22.6012793176972, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI148277065, Length=469, Percent_Identity=22.6012793176972, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI33519430, Length=469, Percent_Identity=22.6012793176972, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI33519428, Length=469, Percent_Identity=22.6012793176972, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI33519426, Length=469, Percent_Identity=22.6012793176972, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI291045268, Length=425, Percent_Identity=24, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1786307, Length=465, Percent_Identity=38.2795698924731, Blast_Score=267, Evalue=8e-73,
Organism=Escherichia coli, GI87082354, Length=467, Percent_Identity=29.7644539614561, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI87081717, Length=457, Percent_Identity=26.4770240700219, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1789915, Length=437, Percent_Identity=29.9771167048055, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI32565766, Length=475, Percent_Identity=36.2105263157895, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI17557007, Length=495, Percent_Identity=27.4747474747475, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71983429, Length=461, Percent_Identity=28.1995661605206, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71983419, Length=461, Percent_Identity=28.1995661605206, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71982272, Length=485, Percent_Identity=23.5051546391753, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321091, Length=466, Percent_Identity=36.2660944206009, Blast_Score=260, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6325240, Length=478, Percent_Identity=29.4979079497908, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6325166, Length=471, Percent_Identity=27.1762208067941, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI21358499, Length=467, Percent_Identity=37.6873661670236, Blast_Score=274, Evalue=8e-74,
Organism=Drosophila melanogaster, GI24640549, Length=472, Percent_Identity=26.9067796610169, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24640553, Length=474, Percent_Identity=26.7932489451477, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24640551, Length=476, Percent_Identity=26.6806722689076, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI17737741, Length=479, Percent_Identity=27.348643006263, Blast_Score=105, Evalue=8e-23,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): DLDH_CHLP8 (O50311)

Other databases:

- EMBL:   Z83933
- EMBL:   CP001099
- PIR:   T17191
- RefSeq:   YP_001998343.1
- ProteinModelPortal:   O50311
- GeneID:   6419649
- GenomeReviews:   CP001099_GR
- KEGG:   cpc:Cpar_0724
- HOGENOM:   HBG515043
- OMA:   EMAWFYA
- ProtClustDB:   CLSK637692
- BRENDA:   1.8.1.4
- GO:   GO:0005737
- GO:   GO:0006096
- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327
- Gene3D:   G3DSA:3.30.390.30
- PRINTS:   PR00368
- TIGRFAMs:   TIGR01350

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim; SSF55424 FAD/NAD-linked_reductase_dimer

EC number: =1.8.1.4

Molecular weight: Translated: 48756; Mature: 48756

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: ACT_SITE 450-450 BINDING 57-57 BINDING 120-120 BINDING 209-209 BINDING 317-317 BINDING 325-325

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQSESSSAQFDVAVIGSGPGGYEAALHAARHGMKVCLVEKASLGGVCVNWGCIPTKALL
CCCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCEEEECCCCCHHHHH
RSAEVYDLAKNPSEFGVNVSELSFDLAQAVKRSRKVSLKSSKGVEFMLKKAKVEVWRGEA
HHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHCEECCCCCCHHHHHHHHEEEEEECCE
VLTGSKGVKVTAEDGSERSLEAANIIVATGAQPRVIPGLEPDGKKIITSREALILKDVPE
EEECCCCEEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEECCCEEEEECCCC
SMIVVGGGAIGVEMAWFYAKAGAKVTIVELMPRLLPAEEAEVSEALKRSFEKVDITVQCG
EEEEEECCCHHHEEEEEEECCCCEEEHHHHHHHHCCCCHHHHHHHHHHCHHHEEEEEEEC
AKLGNVAISEFGVNADLLAEGKEPQKIEASCMLVAVGVTGVIDGLGLDAAGIETERGFIR
CCCCCEEHHHCCCCHHEEECCCCCCEECCEEEEEEECHHHHHHCCCCCCCCCCCCCCCEE
TDELCRTSASGIYAIGDVRGGMLLAHKASAEAAIAVEAIAGKLPEPLSEPLIPRCVYAQP
CHHHHHCCCCCEEEEECCCCCEEEEEECCCCHHEEHHHHHCCCCCCCCCCCCCEEEECCC
SVASVGLTEEAAIAAGYKVLVGRSQFAASGKANAYGQLEGFVKLVFNAETGKMLGGHLIG
CCCCCCCCCHHHHHCCEEEEEECHHHCCCCCCCCCHHHCEEEEEEEECCCCCCCCCEEEC
HDAVELIGELGLACRYGVTAEGLVGTVHAHPTLSETVREAAFAALQSKG
HHHHHHHHHHCCEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQQSESSSAQFDVAVIGSGPGGYEAALHAARHGMKVCLVEKASLGGVCVNWGCIPTKALL
CCCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCEEEECCCCCHHHHH
RSAEVYDLAKNPSEFGVNVSELSFDLAQAVKRSRKVSLKSSKGVEFMLKKAKVEVWRGEA
HHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHCEECCCCCCHHHHHHHHEEEEEECCE
VLTGSKGVKVTAEDGSERSLEAANIIVATGAQPRVIPGLEPDGKKIITSREALILKDVPE
EEECCCCEEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEECCCEEEEECCCC
SMIVVGGGAIGVEMAWFYAKAGAKVTIVELMPRLLPAEEAEVSEALKRSFEKVDITVQCG
EEEEEECCCHHHEEEEEEECCCCEEEHHHHHHHHCCCCHHHHHHHHHHCHHHEEEEEEEC
AKLGNVAISEFGVNADLLAEGKEPQKIEASCMLVAVGVTGVIDGLGLDAAGIETERGFIR
CCCCCEEHHHCCCCHHEEECCCCCCEECCEEEEEEECHHHHHHCCCCCCCCCCCCCCCEE
TDELCRTSASGIYAIGDVRGGMLLAHKASAEAAIAVEAIAGKLPEPLSEPLIPRCVYAQP
CHHHHHCCCCCEEEEECCCCCEEEEEECCCCHHEEHHHHHCCCCCCCCCCCCCEEEECCC
SVASVGLTEEAAIAAGYKVLVGRSQFAASGKANAYGQLEGFVKLVFNAETGKMLGGHLIG
CCCCCCCCCHHHHHCCEEEEEECHHHCCCCCCCCCHHHCEEEEEEEECCCCCCCCCEEEC
HDAVELIGELGLACRYGVTAEGLVGTVHAHPTLSETVREAAFAALQSKG
HHHHHHHHHHCCEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA