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The map label for this gene is ybjS [C]

Identifier: 193212263

GI number: 193212263

Start: 640521

End: 641528

Strand: Direct

Name: ybjS [C]

Synonym: Cpar_0596

Alternate gene names: 193212263

Gene position: 640521-641528 (Clockwise)

Preceding gene: 193212262

Following gene: 193212264

Centisome position: 27.98

GC content: 60.52

Gene sequence:

>1008_bases
ATGAGCAAAACAGTCCTTATCACAGGCGCGTCGGGGTTTATCGGTTCGCATCTGGTAAAGCGTTGCCTGAAGGACGGGTA
TCGCGTCAAGGCGCTGGTGCGGAAGGGAAACTCCTGCATCGAGCCGTTGCGCACTTCCGGCGTCGAGGTGGTGACGGGCG
ATGTGCGCGATGCCGACGCGGTCGATACTGCCGTGCAAGGTTGTGATCTTGTGCTGCACGCAGCGGCGCTGACTTCCGAC
TGGGGGCCGATGCAGGATTTCATCGACATCAACGTCGGCGGCACGCGCAACGTGTGCGAGTCGTCGCTACGGCACGGGGT
TGGGCGGCTGGTGCACATCAGCTCGTTCGAGTGCTTCGACCACCACCTGCTCGGCCGTATCGATGAAGAGACGCCCTGCA
TGCCGCGAAGGCAATCCTATCCCGATACAAAAATCGGTGGAAGCAACGAGGTTTGGGCGGCGATGAAGCGTGGACTTTCA
GCGAGCATTCTCTACCCTGTCTGGGTTTACGGGCCCGGAGATCGCACCCTTTTCCCTTTGCTGGCCGACAGCATACGCAA
ACGGCAGATGTTCTACTGGTCTCGGAATTCGCCGATCAGTCTGGTTTATATCGATAACCTTGTCGAGCTGATCCTGCTTG
CCGCCACTCGTCCCGAGGCCGATGGGGAGGCGTTCATGGCCTGCGACGGCGAACCGATTACCTTCGAGGAGGTGTGCCAG
CGTGTCGCCAAGGCGATTGGCTCGCCGTCGCCGTCGTTGTATCTGCCGTTCGGCCTGGTGCGGAATCTTGCCTGGATGAT
GGAAACGGCCTACCGGATATTCGGAAGCGAAACGCGCCCGCTGCTGACCCGGCAGGCAGTCGATGTGCTCGCCTCGCGCG
CGCTGGCCGATGTGTCGAAATCCCGCAAAATGCTTGGCTGGCAAAGTCTCGTGCCACAGGACGAGGGCATTCGCCGCACG
CTCGACTGGCTGGTGACGGTCGATCCGTCACTGTGGAAGATGAAATAG

Upstream 100 bases:

>100_bases
CGCCGTGCTGGCCTGGACCGAGCGGGTCAATGCGGAGCAGGAGATGGCTGTCGCAGGGGAATAGCGTGAGCGGAAATGTT
TCAAGCAGGATTATAGCTTT

Downstream 100 bases:

>100_bases
AGCATCCGGAGTGCCGGTAAGCGGCTTTGTTTCATCCGAAAAAAAATTCCATTTCCCCGTCGCCCGCAAACCGTTTTGCT
CTGGGCATTGCACGGGTTTA

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: Cholesterol dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-HSD; 3BHSD; 3-beta hydroxysterol dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MSKTVLITGASGFIGSHLVKRCLKDGYRVKALVRKGNSCIEPLRTSGVEVVTGDVRDADAVDTAVQGCDLVLHAAALTSD
WGPMQDFIDINVGGTRNVCESSLRHGVGRLVHISSFECFDHHLLGRIDEETPCMPRRQSYPDTKIGGSNEVWAAMKRGLS
ASILYPVWVYGPGDRTLFPLLADSIRKRQMFYWSRNSPISLVYIDNLVELILLAATRPEADGEAFMACDGEPITFEEVCQ
RVAKAIGSPSPSLYLPFGLVRNLAWMMETAYRIFGSETRPLLTRQAVDVLASRALADVSKSRKMLGWQSLVPQDEGIRRT
LDWLVTVDPSLWKMK

Sequences:

>Translated_335_residues
MSKTVLITGASGFIGSHLVKRCLKDGYRVKALVRKGNSCIEPLRTSGVEVVTGDVRDADAVDTAVQGCDLVLHAAALTSD
WGPMQDFIDINVGGTRNVCESSLRHGVGRLVHISSFECFDHHLLGRIDEETPCMPRRQSYPDTKIGGSNEVWAAMKRGLS
ASILYPVWVYGPGDRTLFPLLADSIRKRQMFYWSRNSPISLVYIDNLVELILLAATRPEADGEAFMACDGEPITFEEVCQ
RVAKAIGSPSPSLYLPFGLVRNLAWMMETAYRIFGSETRPLLTRQAVDVLASRALADVSKSRKMLGWQSLVPQDEGIRRT
LDWLVTVDPSLWKMK
>Mature_334_residues
SKTVLITGASGFIGSHLVKRCLKDGYRVKALVRKGNSCIEPLRTSGVEVVTGDVRDADAVDTAVQGCDLVLHAAALTSDW
GPMQDFIDINVGGTRNVCESSLRHGVGRLVHISSFECFDHHLLGRIDEETPCMPRRQSYPDTKIGGSNEVWAAMKRGLSA
SILYPVWVYGPGDRTLFPLLADSIRKRQMFYWSRNSPISLVYIDNLVELILLAATRPEADGEAFMACDGEPITFEEVCQR
VAKAIGSPSPSLYLPFGLVRNLAWMMETAYRIFGSETRPLLTRQAVDVLASRALADVSKSRKMLGWQSLVPQDEGIRRTL
DWLVTVDPSLWKMK

Specific function: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidation and isomerization of cholesterol, pregnenolone, and dehydroepiandrosterone (DHEA) into cholest-4-en-3-one, progesterone, and androsterone, respectively [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-beta-HSD family [H]

Homologues:

Organism=Homo sapiens, GI116268111, Length=350, Percent_Identity=27.7142857142857, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI193211614, Length=336, Percent_Identity=25, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI8393516, Length=336, Percent_Identity=25, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI310132178, Length=330, Percent_Identity=27.8787878787879, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI310113012, Length=330, Percent_Identity=27.8787878787879, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI239745448, Length=330, Percent_Identity=27.8787878787879, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI19923621, Length=358, Percent_Identity=25.9776536312849, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI87081792, Length=331, Percent_Identity=26.2839879154079, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71987463, Length=327, Percent_Identity=27.217125382263, Blast_Score=81, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6321437, Length=354, Percent_Identity=27.9661016949153, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.1.1.145; =5.3.3.1 [H]

Molecular weight: Translated: 37134; Mature: 37003

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTVLITGASGFIGSHLVKRCLKDGYRVKALVRKGNSCIEPLRTSGVEVVTGDVRDADA
CCCEEEEECCCCHHHHHHHHHHHHCCHHEHHHHHCCCHHHHHHHHCCCEEEECCCCCCHH
VDTAVQGCDLVLHAAALTSDWGPMQDFIDINVGGTRNVCESSLRHGVGRLVHISSFECFD
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCEEEECCHHHHH
HHLLGRIDEETPCMPRRQSYPDTKIGGSNEVWAAMKRGLSASILYPVWVYGPGDRTLFPL
HHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEEEEECCCCCHHHH
LADSIRKRQMFYWSRNSPISLVYIDNLVELILLAATRPEADGEAFMACDGEPITFEEVCQ
HHHHHHHHHHEEEECCCCEEEEEHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHH
RVAKAIGSPSPSLYLPFGLVRNLAWMMETAYRIFGSETRPLLTRQAVDVLASRALADVSK
HHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
SRKMLGWQSLVPQDEGIRRTLDWLVTVDPSLWKMK
HHHHHHHHHCCCCCCHHHHHHHHHEECCCHHHCCC
>Mature Secondary Structure 
SKTVLITGASGFIGSHLVKRCLKDGYRVKALVRKGNSCIEPLRTSGVEVVTGDVRDADA
CCEEEEECCCCHHHHHHHHHHHHCCHHEHHHHHCCCHHHHHHHHCCCEEEECCCCCCHH
VDTAVQGCDLVLHAAALTSDWGPMQDFIDINVGGTRNVCESSLRHGVGRLVHISSFECFD
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCEEEECCHHHHH
HHLLGRIDEETPCMPRRQSYPDTKIGGSNEVWAAMKRGLSASILYPVWVYGPGDRTLFPL
HHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEEEEECCCCCHHHH
LADSIRKRQMFYWSRNSPISLVYIDNLVELILLAATRPEADGEAFMACDGEPITFEEVCQ
HHHHHHHHHHEEEECCCCEEEEEHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHH
RVAKAIGSPSPSLYLPFGLVRNLAWMMETAYRIFGSETRPLLTRQAVDVLASRALADVSK
HHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
SRKMLGWQSLVPQDEGIRRTLDWLVTVDPSLWKMK
HHHHHHHHHCCCCCCHHHHHHHHHEECCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]