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The map label for this gene is gcvH

Identifier: 193212231

GI number: 193212231

Start: 606886

End: 607269

Strand: Direct

Name: gcvH

Synonym: Cpar_0562

Alternate gene names: 193212231

Gene position: 606886-607269 (Clockwise)

Preceding gene: 193212230

Following gene: 193212232

Centisome position: 26.51

GC content: 58.07

Gene sequence:

>384_bases
ATGAACATCCCTGATAATCTTCGCTATACCAAAGACCACGAATGGCTCCAGCTTCTCGATGATGGCGTGACCGCCGTGGT
TGGCATCACCGATTTCGCGCAGTCTGAACTTGGCGACATCGTGTTCGTCGAAACCAAGCCGGTCGGCACCAAGGTCGCGG
CGCACGGCGCGTTTGGCACTGTTGAGGCGGTCAAGACTGTGGCCGATCTTTTCGCTCCGGTGGCGGGTGAGATCGTCGAG
GTCAATGGTGCACTCGACGATGCGGCGATTGTCAACTCCGATCCCTACAACGAGGGCTGGATTGTCAAGATGAAGCTCGA
CAACGCTGCCGAGGTCGAGTCGCTGCTCTCACCGGCGGATTACAGCGCCCTGATCGGCGAATAA

Upstream 100 bases:

>100_bases
ACGTCCAATCCGACCGATCTTTCCGCGATTCTGAAAAAATGTTACCTTCAAGTTCATTTGTAACTCTTCAATTGCATAGC
AACTTAAATTGCGTTCTGCC

Downstream 100 bases:

>100_bases
GGATTATCCAGAATTTCAATACGCAACCATGCCTTTCATTGTCAATACCGATGCTGAGCGGGCCGAGATGCTTCGCGAAA
TCGGCGTCGAGAATTTCGAG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 127

Protein sequence:

>127_residues
MNIPDNLRYTKDHEWLQLLDDGVTAVVGITDFAQSELGDIVFVETKPVGTKVAAHGAFGTVEAVKTVADLFAPVAGEIVE
VNGALDDAAIVNSDPYNEGWIVKMKLDNAAEVESLLSPADYSALIGE

Sequences:

>Translated_127_residues
MNIPDNLRYTKDHEWLQLLDDGVTAVVGITDFAQSELGDIVFVETKPVGTKVAAHGAFGTVEAVKTVADLFAPVAGEIVE
VNGALDDAAIVNSDPYNEGWIVKMKLDNAAEVESLLSPADYSALIGE
>Mature_127_residues
MNIPDNLRYTKDHEWLQLLDDGVTAVVGITDFAQSELGDIVFVETKPVGTKVAAHGAFGTVEAVKTVADLFAPVAGEIVE
VNGALDDAAIVNSDPYNEGWIVKMKLDNAAEVESLLSPADYSALIGE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=119, Percent_Identity=38.655462184874, Blast_Score=100, Evalue=4e-22,
Organism=Homo sapiens, GI89057342, Length=119, Percent_Identity=37.8151260504202, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789271, Length=125, Percent_Identity=49.6, Blast_Score=116, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17507493, Length=117, Percent_Identity=41.025641025641, Blast_Score=102, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17551294, Length=120, Percent_Identity=37.5, Blast_Score=93, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6319272, Length=126, Percent_Identity=38.8888888888889, Blast_Score=97, Evalue=5e-22,
Organism=Drosophila melanogaster, GI17865652, Length=120, Percent_Identity=37.5, Blast_Score=99, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_CHLP8 (B3QM31)

Other databases:

- EMBL:   CP001099
- RefSeq:   YP_001998184.1
- GeneID:   6419476
- GenomeReviews:   CP001099_GR
- KEGG:   cpc:Cpar_0562
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- ProtClustDB:   PRK01202
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13503; Mature: 13503

Theoretical pI: Translated: 3.86; Mature: 3.86

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIPDNLRYTKDHEWLQLLDDGVTAVVGITDFAQSELGDIVFVETKPVGTKVAAHGAFGT
CCCCCCCCCCCCHHHHHHHHCCCEEEEEHHHHHHHCCCCEEEEECCCCCCEEECCCCCHH
VEAVKTVADLFAPVAGEIVEVNGALDDAAIVNSDPYNEGWIVKMKLDNAAEVESLLSPAD
HHHHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCCCCCEEEEEECCCHHHHHHHCCCCC
YSALIGE
CHHHCCC
>Mature Secondary Structure
MNIPDNLRYTKDHEWLQLLDDGVTAVVGITDFAQSELGDIVFVETKPVGTKVAAHGAFGT
CCCCCCCCCCCCHHHHHHHHCCCEEEEEHHHHHHHCCCCEEEEECCCCCCEEECCCCCHH
VEAVKTVADLFAPVAGEIVEVNGALDDAAIVNSDPYNEGWIVKMKLDNAAEVESLLSPAD
HHHHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCCCCCEEEEEECCCHHHHHHHCCCCC
YSALIGE
CHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA