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The map label for this gene is hisH [H]

Identifier: 193212139

GI number: 193212139

Start: 500934

End: 501542

Strand: Direct

Name: hisH [H]

Synonym: Cpar_0470

Alternate gene names: 193212139

Gene position: 500934-501542 (Clockwise)

Preceding gene: 193212131

Following gene: 193212140

Centisome position: 21.88

GC content: 54.35

Gene sequence:

>609_bases
ATGATATTTATTGCCGATTACGGCGCCGGAAATCTCAGGTCGGTGCACAAGGCGTTCGAGTTTCTCAGTATCGATGCGGT
TGTCAGCGACAAGGCTTCGGAGATGAGCCGTTACGAAAAGGTGCTCATTCCAGGCGTTGGCGCTTATGGCCCTGCGATGG
AGGCGTTCAACCAGCATGGTTTCGGCGATGCGATCGGGGAGCACATCGACAAGGGGCGGAGCGTGCTCGGCATCTGCCTC
GGCATGCAGCTCTTTCTGACCGAAAGCGAAGAGATGGGCACGAATCAGGGGCTCGATCTCGTACCCGGAAAGGTGCTTCA
TTTTCCGCGTGGCGGTGACAAAATTCCGCAGATCGGCTGGAACACGATCAATCTGTGCCGCAAAGAGTCGGTGCTGTTCC
GCGGTATTCCGGACGAGTCGTGGTTCTACTTCGTCCACTCCTACTATTGCAGCCCCGTCGATCCGGCAAGTATCGCTGCC
ACGACCGAGTACGCCGGAAAAAAGTTTTGTTCGGCCATTGAAAAAAATGGTATTTTCGCAGTTCAGTTCCACCCGGAAAA
GAGCTCCGAAGCGGGCTTGCTGGTGTTGAAGAATTTTGCAGAGTTTTAA

Upstream 100 bases:

>100_bases
GGCAAGAACTTCTTAATTGAAAGCCGTGTTCACGCTTTTTCGACCTGTGGGTGCTTTTTTCTCCATCCGGTGGTCATCAG
TCACGCTTAATTTTCTGTTC

Downstream 100 bases:

>100_bases
GCTGTTGAGGGGTTTACGCTATGTTGATCATACCCGCTATCGATATTAAGGAAGGCAAGTGCGTCAGACTGACCAGGGGT
GATTTTGCTCAGAAAAAGAT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MIFIADYGAGNLRSVHKAFEFLSIDAVVSDKASEMSRYEKVLIPGVGAYGPAMEAFNQHGFGDAIGEHIDKGRSVLGICL
GMQLFLTESEEMGTNQGLDLVPGKVLHFPRGGDKIPQIGWNTINLCRKESVLFRGIPDESWFYFVHSYYCSPVDPASIAA
TTEYAGKKFCSAIEKNGIFAVQFHPEKSSEAGLLVLKNFAEF

Sequences:

>Translated_202_residues
MIFIADYGAGNLRSVHKAFEFLSIDAVVSDKASEMSRYEKVLIPGVGAYGPAMEAFNQHGFGDAIGEHIDKGRSVLGICL
GMQLFLTESEEMGTNQGLDLVPGKVLHFPRGGDKIPQIGWNTINLCRKESVLFRGIPDESWFYFVHSYYCSPVDPASIAA
TTEYAGKKFCSAIEKNGIFAVQFHPEKSSEAGLLVLKNFAEF
>Mature_202_residues
MIFIADYGAGNLRSVHKAFEFLSIDAVVSDKASEMSRYEKVLIPGVGAYGPAMEAFNQHGFGDAIGEHIDKGRSVLGICL
GMQLFLTESEEMGTNQGLDLVPGKVLHFPRGGDKIPQIGWNTINLCRKESVLFRGIPDESWFYFVHSYYCSPVDPASIAA
TTEYAGKKFCSAIEKNGIFAVQFHPEKSSEAGLLVLKNFAEF

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=201, Percent_Identity=36.81592039801, Blast_Score=114, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6319725, Length=209, Percent_Identity=33.9712918660287, Blast_Score=121, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22182; Mature: 22182

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFIADYGAGNLRSVHKAFEFLSIDAVVSDKASEMSRYEKVLIPGVGAYGPAMEAFNQHG
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHCCC
FGDAIGEHIDKGRSVLGICLGMQLFLTESEEMGTNQGLDLVPGKVLHFPRGGDKIPQIGW
CHHHHHHHHHCCHHHHHHHHHHHHEEECCHHCCCCCCCCCCCCCEEECCCCCCCCCCCCC
NTINLCRKESVLFRGIPDESWFYFVHSYYCSPVDPASIAATTEYAGKKFCSAIEKNGIFA
CHHHHHHHCCCEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEE
VQFHPEKSSEAGLLVLKNFAEF
EEECCCCCCCCCEEEEHHHHCC
>Mature Secondary Structure
MIFIADYGAGNLRSVHKAFEFLSIDAVVSDKASEMSRYEKVLIPGVGAYGPAMEAFNQHG
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHCCC
FGDAIGEHIDKGRSVLGICLGMQLFLTESEEMGTNQGLDLVPGKVLHFPRGGDKIPQIGW
CHHHHHHHHHCCHHHHHHHHHHHHEEECCHHCCCCCCCCCCCCCEEECCCCCCCCCCCCC
NTINLCRKESVLFRGIPDESWFYFVHSYYCSPVDPASIAATTEYAGKKFCSAIEKNGIFA
CHHHHHHHCCCEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEE
VQFHPEKSSEAGLLVLKNFAEF
EEECCCCCCCCCEEEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]