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The map label for this gene is ssb [H]

Identifier: 192291528

GI number: 192291528

Start: 3399306

End: 3399809

Strand: Reverse

Name: ssb [H]

Synonym: Rpal_3154

Alternate gene names: 192291528

Gene position: 3399809-3399306 (Counterclockwise)

Preceding gene: 192291533

Following gene: 192291524

Centisome position: 59.19

GC content: 64.88

Gene sequence:

>504_bases
ATGGCGGGTAGCGTGAACAAGGTGATCCTGGTCGGGAATCTCGGTGCCGATCCGGAGATCAAGCGGACCCAGGACGGGCG
TCCGATTGCCAACCTGCGGATCGCCACCTCGGAGTCCTGGCGCGATCGCGCCACCGGCGAGCGCAAGGAAAAGACCGAGT
GGCATCGCGTGGTGATCTTCAATGAGGGATTGTGCAAGGTCGCGGAGCAGTACCTGAGAAAGGGCGCGAAGGTTTACATC
GAAGGCCAACTGCAGACACGCAAATGGACCGATCAGAGCGGCGCTGAGCGCTACAGCACCGAGGTTGTGCTGCAGAACTT
CAACTCGAATCTGACCATGCTCGACGGACGCAGCGGCGGCGGAGGTGGCGGCGGCTATGGCGACGACAACTCCGGCGGCG
ATTTCGGCTCCAGCGGCCCCTCGGGTGGCGGCGGCGCACGGCGGCCGATGCCGGCCAGCAGCGGCGGCGGCCGCAGCGAT
ATGGACGACGACATTCCGTTCTGA

Upstream 100 bases:

>100_bases
CGCCGAGCTTTGGCGTGAGCAGCGGCAGAGCGGAAGGCGGGCAATGGCCGGCCGGGTCGCGGGCGCCGCGCAGTCAGTAT
GGTTTTAAGAGGAGACGTCG

Downstream 100 bases:

>100_bases
GCCCACGGGTTCTCAGCCATTCCTTTCCGGCCGTGCTCGGCTGGGACGGGATACGAGACGACGGCCGCCGCGCCCCATCG
CGGCGGCCGTCGTCGTTTTG

Product: single-stranded DNA-binding protein

Products: NA

Alternate protein names: SSB; Helix-destabilizing protein [H]

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MAGSVNKVILVGNLGADPEIKRTQDGRPIANLRIATSESWRDRATGERKEKTEWHRVVIFNEGLCKVAEQYLRKGAKVYI
EGQLQTRKWTDQSGAERYSTEVVLQNFNSNLTMLDGRSGGGGGGGYGDDNSGGDFGSSGPSGGGGARRPMPASSGGGRSD
MDDDIPF

Sequences:

>Translated_167_residues
MAGSVNKVILVGNLGADPEIKRTQDGRPIANLRIATSESWRDRATGERKEKTEWHRVVIFNEGLCKVAEQYLRKGAKVYI
EGQLQTRKWTDQSGAERYSTEVVLQNFNSNLTMLDGRSGGGGGGGYGDDNSGGDFGSSGPSGGGGARRPMPASSGGGRSD
MDDDIPF
>Mature_166_residues
AGSVNKVILVGNLGADPEIKRTQDGRPIANLRIATSESWRDRATGERKEKTEWHRVVIFNEGLCKVAEQYLRKGAKVYIE
GQLQTRKWTDQSGAERYSTEVVLQNFNSNLTMLDGRSGGGGGGGYGDDNSGGDFGSSGPSGGGGARRPMPASSGGGRSDM
DDDIPF

Specific function: This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair [H]

COG id: COG0629

COG function: function code L; Single-stranded DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SSB domain [H]

Homologues:

Organism=Homo sapiens, GI4507231, Length=112, Percent_Identity=32.1428571428571, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1790494, Length=175, Percent_Identity=53.1428571428571, Blast_Score=160, Evalue=3e-41,
Organism=Drosophila melanogaster, GI85725234, Length=100, Percent_Identity=32, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24647376, Length=100, Percent_Identity=32, Blast_Score=71, Evalue=4e-13,

Paralogues:

None

Copy number: 1622 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000424
- InterPro:   IPR011344 [H]

Pfam domain/function: PF00436 SSB [H]

EC number: NA

Molecular weight: Translated: 17828; Mature: 17697

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS50935 SSB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGSVNKVILVGNLGADPEIKRTQDGRPIANLRIATSESWRDRATGERKEKTEWHRVVIF
CCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCHHHHCCCCCCCCCCEEEEEEE
NEGLCKVAEQYLRKGAKVYIEGQLQTRKWTDQSGAERYSTEVVLQNFNSNLTMLDGRSGG
CCCHHHHHHHHHHCCCEEEEEECEECCCCCCCCCHHHHHHHHHEECCCCCEEEEECCCCC
GGGGGYGDDNSGGDFGSSGPSGGGGARRPMPASSGGGRSDMDDDIPF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AGSVNKVILVGNLGADPEIKRTQDGRPIANLRIATSESWRDRATGERKEKTEWHRVVIF
CCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCHHHHCCCCCCCCCCEEEEEEE
NEGLCKVAEQYLRKGAKVYIEGQLQTRKWTDQSGAERYSTEVVLQNFNSNLTMLDGRSGG
CCCHHHHHHHHHHCCCEEEEEECEECCCCCCCCCHHHHHHHHHEECCCCCEEEEECCCCC
GGGGGYGDDNSGGDFGSSGPSGGGGARRPMPASSGGGRSDMDDDIPF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]