Click here to switch to the map view.

The map label for this gene is glyA2 [H]

Identifier: 192291511

GI number: 192291511

Start: 3381219

End: 3382523

Strand: Reverse

Name: glyA2 [H]

Synonym: Rpal_3135

Alternate gene names: 192291511

Gene position: 3382523-3381219 (Counterclockwise)

Preceding gene: 192291512

Following gene: 192291510

Centisome position: 58.89

GC content: 64.06

Gene sequence:

>1305_bases
ATGGGTCCATCCGTTGCGCCGCACGCTGTCCACACCATCGCAAACCCAGGCGTGAGCGCCGCCGGCCTAGTCGCGGCTGA
TCACGCTGTCGCGGCCGCGATCGCCAATGAAGAAACCCGCCAGCGCGACTCGATCGAGCTGATCGCCTCGGAGAATTTCG
TCAGCCGGGCGGTCCTCGATGCGCAGGGATCGGTACTGACTAACAAATATGCCGAAGGCTATCCACATCGTCGCTACTAC
GGCGGTTGCGCGAATGTCGACGCGATCGAGGATCTGGCGATCGCCCGTGTCAATCAGCTGTTCGGCAGCGCCTACGCCAA
CGTCCAGCCGCATTCGGGAAGCCAAGCCAATCAGGCGGTGTTTCTCGCACTGCTCGCACCCGGCGATACGATCCTTGGCC
TGGATTTGAAGGCCGGCGGCCATCTGACCCATGGGGCGCCGGTCAACATGTCCGGCCGCTGGTTCAAGGCCGTCAGCTAC
GGCGTCGACCCCGAGACTCATCGCATCGACATGGATCAGGTGGCGGCACAGGCGCGCCAGCATCGCCCAAGGCTGCTGAT
TGCTGGCGGGTCGGCCTATCCGCGGATCATCGATTTCGGCCGCTTCCGCCAGATCGCCGACGAGGTTGGAGCGATCCTGA
TGGTCGACATGGCCCACTTCGCCGGGCTCGTGGCCGGAGGCGTTTATCCGTCGCCTGTTCCGTTTGCCGATGTCGTTACC
TCGACGACGCACAAGACGCTTCGTGGTCCGCGCGGCGGGTTCGTTCTGACCAACGATGCGAACATCGCCAAGAAGATCAA
TTCAGCGACGTTCCCCGGGCTTCAGGGCGGCCCGCTGATGCATGTGATTGCCGCGAAGGCCGTCGCTTTTGGCGAGGCGC
TGCAACCGGAGTTCGGAGCCTATGCGCAGGCCGTCGTCGAGAATTGCCGCGTGCTGGCGCAAGCGCTTGCGGATGGCGGG
CTGACGATCACCTCGGGCGGCACCGATTGCCATCTCGCCGTCGTCGATCTGCGACCATTCGGCGTGACCGGTAATATCGC
CGAGCAGGCTTTGGAGTCGGTAGGGATCACGCTCAACAAGAACGCGATCCCGAACGATCCCGAGAAACCAATGGTGACGT
CGGGTATCCGTGTGGGAACGGCTGCCGGCACGTCCCGCGGCTTCGGCGCGGACCAGTATCGCGAGATTGCCGGGCTTGTC
CTCGAAACGCTCCACGCCGTCCGCGCCGGGACGCTGGATGCAGAGCGGCAAGCGATCAACAAGCGCGTTCGGCGACTGGC
CGCGAGCTTTCCGCTCCCTTACTGA

Upstream 100 bases:

>100_bases
GAGGGGATAACCAGACCACGCCCGTGCTAGATCCTGGTGACCGACGCAGCACGTGTCTTCTGATGCGCGCGGCAAGTCGT
CGGCATCAAGGAGATCAGCC

Downstream 100 bases:

>100_bases
TCAGGAGTATTCGTGTCACGATGCCTGCCAGTCCCGACCTCTTGCCGGACGAGATCGCCCGTTTCGGCACTGATCCCGCC
GCGGCCTTTGCGGCGAACCT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT 2; Serine methylase 2 [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MGPSVAPHAVHTIANPGVSAAGLVAADHAVAAAIANEETRQRDSIELIASENFVSRAVLDAQGSVLTNKYAEGYPHRRYY
GGCANVDAIEDLAIARVNQLFGSAYANVQPHSGSQANQAVFLALLAPGDTILGLDLKAGGHLTHGAPVNMSGRWFKAVSY
GVDPETHRIDMDQVAAQARQHRPRLLIAGGSAYPRIIDFGRFRQIADEVGAILMVDMAHFAGLVAGGVYPSPVPFADVVT
STTHKTLRGPRGGFVLTNDANIAKKINSATFPGLQGGPLMHVIAAKAVAFGEALQPEFGAYAQAVVENCRVLAQALADGG
LTITSGGTDCHLAVVDLRPFGVTGNIAEQALESVGITLNKNAIPNDPEKPMVTSGIRVGTAAGTSRGFGADQYREIAGLV
LETLHAVRAGTLDAERQAINKRVRRLAASFPLPY

Sequences:

>Translated_434_residues
MGPSVAPHAVHTIANPGVSAAGLVAADHAVAAAIANEETRQRDSIELIASENFVSRAVLDAQGSVLTNKYAEGYPHRRYY
GGCANVDAIEDLAIARVNQLFGSAYANVQPHSGSQANQAVFLALLAPGDTILGLDLKAGGHLTHGAPVNMSGRWFKAVSY
GVDPETHRIDMDQVAAQARQHRPRLLIAGGSAYPRIIDFGRFRQIADEVGAILMVDMAHFAGLVAGGVYPSPVPFADVVT
STTHKTLRGPRGGFVLTNDANIAKKINSATFPGLQGGPLMHVIAAKAVAFGEALQPEFGAYAQAVVENCRVLAQALADGG
LTITSGGTDCHLAVVDLRPFGVTGNIAEQALESVGITLNKNAIPNDPEKPMVTSGIRVGTAAGTSRGFGADQYREIAGLV
LETLHAVRAGTLDAERQAINKRVRRLAASFPLPY
>Mature_433_residues
GPSVAPHAVHTIANPGVSAAGLVAADHAVAAAIANEETRQRDSIELIASENFVSRAVLDAQGSVLTNKYAEGYPHRRYYG
GCANVDAIEDLAIARVNQLFGSAYANVQPHSGSQANQAVFLALLAPGDTILGLDLKAGGHLTHGAPVNMSGRWFKAVSYG
VDPETHRIDMDQVAAQARQHRPRLLIAGGSAYPRIIDFGRFRQIADEVGAILMVDMAHFAGLVAGGVYPSPVPFADVVTS
TTHKTLRGPRGGFVLTNDANIAKKINSATFPGLQGGPLMHVIAAKAVAFGEALQPEFGAYAQAVVENCRVLAQALADGGL
TITSGGTDCHLAVVDLRPFGVTGNIAEQALESVGITLNKNAIPNDPEKPMVTSGIRVGTAAGTSRGFGADQYREIAGLVL
ETLHAVRAGTLDAERQAINKRVRRLAASFPLPY

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI261862352, Length=452, Percent_Identity=44.2477876106195, Blast_Score=344, Evalue=1e-94,
Organism=Homo sapiens, GI261862350, Length=452, Percent_Identity=44.2477876106195, Blast_Score=344, Evalue=1e-94,
Organism=Homo sapiens, GI261862348, Length=452, Percent_Identity=44.2477876106195, Blast_Score=344, Evalue=1e-94,
Organism=Homo sapiens, GI19923315, Length=462, Percent_Identity=43.9393939393939, Blast_Score=344, Evalue=1e-94,
Organism=Homo sapiens, GI261862346, Length=462, Percent_Identity=42.8571428571429, Blast_Score=326, Evalue=2e-89,
Organism=Homo sapiens, GI22547186, Length=457, Percent_Identity=43.9824945295405, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI22547189, Length=443, Percent_Identity=41.7607223476298, Blast_Score=288, Evalue=7e-78,
Organism=Escherichia coli, GI1788902, Length=408, Percent_Identity=53.6764705882353, Blast_Score=422, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI25144732, Length=398, Percent_Identity=45.7286432160804, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI25144729, Length=398, Percent_Identity=45.7286432160804, Blast_Score=332, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6319739, Length=390, Percent_Identity=44.3589743589744, Blast_Score=329, Evalue=5e-91,
Organism=Saccharomyces cerevisiae, GI6323087, Length=401, Percent_Identity=42.8927680798005, Blast_Score=317, Evalue=2e-87,
Organism=Drosophila melanogaster, GI24640005, Length=466, Percent_Identity=43.1330472103004, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI221329721, Length=453, Percent_Identity=44.1501103752759, Blast_Score=355, Evalue=3e-98,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 45472; Mature: 45341

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPSVAPHAVHTIANPGVSAAGLVAADHAVAAAIANEETRQRDSIELIASENFVSRAVLD
CCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHCCCCEEEEECCCHHHHHHHH
AQGSVLTNKYAEGYPHRRYYGGCANVDAIEDLAIARVNQLFGSAYANVQPHSGSQANQAV
CCCCCHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
FLALLAPGDTILGLDLKAGGHLTHGAPVNMSGRWFKAVSYGVDPETHRIDMDQVAAQARQ
EEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEECHHHHHHHHHH
HRPRLLIAGGSAYPRIIDFGRFRQIADEVGAILMVDMAHFAGLVAGGVYPSPVPFADVVT
CCCEEEEECCCCCCHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHCCCCCCCCCHHHHHH
STTHKTLRGPRGGFVLTNDANIAKKINSATFPGLQGGPLMHVIAAKAVAFGEALQPEFGA
HHHHHHHCCCCCCEEEECCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHH
YAQAVVENCRVLAQALADGGLTITSGGTDCHLAVVDLRPFGVTGNIAEQALESVGITLNK
HHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEECCCCCCHHHHHHHHHCCCEEEC
NAIPNDPEKPMVTSGIRVGTAAGTSRGFGADQYREIAGLVLETLHAVRAGTLDAERQAIN
CCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KRVRRLAASFPLPY
HHHHHHHHHCCCCC
>Mature Secondary Structure 
GPSVAPHAVHTIANPGVSAAGLVAADHAVAAAIANEETRQRDSIELIASENFVSRAVLD
CCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHCCCCEEEEECCCHHHHHHHH
AQGSVLTNKYAEGYPHRRYYGGCANVDAIEDLAIARVNQLFGSAYANVQPHSGSQANQAV
CCCCCHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
FLALLAPGDTILGLDLKAGGHLTHGAPVNMSGRWFKAVSYGVDPETHRIDMDQVAAQARQ
EEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEECHHHHHHHHHH
HRPRLLIAGGSAYPRIIDFGRFRQIADEVGAILMVDMAHFAGLVAGGVYPSPVPFADVVT
CCCEEEEECCCCCCHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHCCCCCCCCCHHHHHH
STTHKTLRGPRGGFVLTNDANIAKKINSATFPGLQGGPLMHVIAAKAVAFGEALQPEFGA
HHHHHHHCCCCCCEEEECCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHH
YAQAVVENCRVLAQALADGGLTITSGGTDCHLAVVDLRPFGVTGNIAEQALESVGITLNK
HHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEECCCCCCHHHHHHHHHCCCEEEC
NAIPNDPEKPMVTSGIRVGTAAGTSRGFGADQYREIAGLVLETLHAVRAGTLDAERQAIN
CCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KRVRRLAASFPLPY
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA