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The map label for this gene is alkK [H]

Identifier: 192291495

GI number: 192291495

Start: 3362955

End: 3364604

Strand: Reverse

Name: alkK [H]

Synonym: Rpal_3119

Alternate gene names: 192291495

Gene position: 3364604-3362955 (Counterclockwise)

Preceding gene: 192291496

Following gene: 192291488

Centisome position: 58.58

GC content: 64.73

Gene sequence:

>1650_bases
ATGAGCACCCCGCAAGGTCACTACAGCATCGGTCTGGACAAGACCCCGGCCAACTTCGTGCCGCTGTCGCCGCTGAGCTT
CCTGGAGCGCACCGCCAACGTTTATCCGGAAATGACCTCCGTGGTGTACGAGGGGCGGCACTACACCTGGAAAGAGACCC
GCGCGCGCTGCCGCCGCTTCGCCTCCTGGCTGACCCGCAGCGGCATCGGCCGCGGCGACACCGTCGCGGTGATGCTGCCC
AACGTGCCGGCGATGGTCGAAGTGCATTTCGCGGTGCCGATGGCCGGCGCGGTGCTGAACGCGCTCAACATTCGGCTCGA
TGCTGCGGCGATCGCCTTCCAACTCGATCACGGCGGCGCCAAGATCATCCTGGTCGATCCGGAGTTCTCCAGCGTCGTCG
CCGAGGCGCTGAAGCTGATGACCAAGCCGAAGCCGCTGGTGATCGACGTCGACGACAAGATGTATCCCGGCAGCCACCGT
ATCGGCGAACTGGAATACGAATACGCCGTCGCGTCCGGCGATCCGACCTTCGCCGGCCATCGCCCCGAGGAAGAGTGGGA
CGCGATCTCGCTCGGCTACACCTCCGGCACCACCGGCAATCCGAAGGGCGTCGTCACGCATCATCGCGGCGCGTATCTCA
ACGCCGTCAGCAACATTCTCGCCGGCAATCTCGGCCAGCATCCGGTGTATCTGTGGACGCTGCCGATGTTCCACTGCAAC
GGCTGGTGCTTCCCGTGGACGCTCGCGGCCGCGGCCGGCATCAACGTCTGCCTGCGCAAGGTCGACCCGGCCAAGATCTT
CGAACTGATCCCGAAGCACGGCGTCACTCATATGTGCGGCGCACCGATCGTCTACAACGCACTGATCAACGCACCTGAAG
CGCCGAAGGGCGCTGCGACGAAGCCCGTGGTCGGCCTGATCGCCGGCGCCGCGCCGCCGATGGCGGTGCTGGCCGGCGCC
GAGACCATCGGTATCAAGCTGACGCATGTGTACGGCCTGACCGAAGTGTACGGCCCCGCCTCGGTCTGCGCCGAGCAGCC
CGGTTGGGACGAGCTGCCGGTCGCCGAGCGCGCCAAGCTGAAGCGTCGCCAGGGCGTGCCCTACCCGATGCAGGAAGCCG
TCACCGTGCTCAATCCTGAGACGATGCAGGAAGTGCCGCGCGACGGCGAGACCATCGGCGAGGTGATGTTCCGCGGCAAT
ATCGTGATGAAGGGCTATCTAAAGAACGAGAAGGCCACCAAGGAAGCGCTCGCCGGCGGCTGGTTTCACACCGGCGACCT
CGGCGTGCTCGATGCCGACGGCTACGTCATCATCAAGGACCGCTCCAAGGACATCATCATCTCGGGCGGCGAGAACGTCT
CGTCGGTCGAGGTCGAAGACGTGCTGTACAAGCACCCGGCGATCCTGTTCGCCGCCGTGGTCGCCAAACCTGATCCGAAA
TGGGGCGAAGTCCCCTGCGCCTTCGTCGAGCTGAAAGACGGCGCCAGCGCCACCGAAGCCGAGATCATCGCCTATTGCCG
CGAGCACCTGCCCGGCTTCAAGACCCCGAAGAGCATCGTGTTCTCGGCCATCCCCAAGACTTCGACCGGCAAGATCCAGA
AATTCATGCTGCGCGATCAGGTGAAGTCGGCGAAGGCGATTTTGGAGTAA

Upstream 100 bases:

>100_bases
GATCGTCGTCGCGGCGATCCCGTGGATCTCGATTGGATTCTTGTAGAGGCGCTGCGGGGAGATTGCAGCGGCGCCGTCGT
GAAGTGAGAGGCAGATCGTC

Downstream 100 bases:

>100_bases
TTGGTTTTCGTCATTCCGGGGCGCGCGAAGCGCGAACCCGGAATCTCGAGGTTGTTAGTGACGTTATTAGTAAGGGCTGT
CGAAGCCGTGCGCGTTTCAG

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MSTPQGHYSIGLDKTPANFVPLSPLSFLERTANVYPEMTSVVYEGRHYTWKETRARCRRFASWLTRSGIGRGDTVAVMLP
NVPAMVEVHFAVPMAGAVLNALNIRLDAAAIAFQLDHGGAKIILVDPEFSSVVAEALKLMTKPKPLVIDVDDKMYPGSHR
IGELEYEYAVASGDPTFAGHRPEEEWDAISLGYTSGTTGNPKGVVTHHRGAYLNAVSNILAGNLGQHPVYLWTLPMFHCN
GWCFPWTLAAAAGINVCLRKVDPAKIFELIPKHGVTHMCGAPIVYNALINAPEAPKGAATKPVVGLIAGAAPPMAVLAGA
ETIGIKLTHVYGLTEVYGPASVCAEQPGWDELPVAERAKLKRRQGVPYPMQEAVTVLNPETMQEVPRDGETIGEVMFRGN
IVMKGYLKNEKATKEALAGGWFHTGDLGVLDADGYVIIKDRSKDIIISGGENVSSVEVEDVLYKHPAILFAAVVAKPDPK
WGEVPCAFVELKDGASATEAEIIAYCREHLPGFKTPKSIVFSAIPKTSTGKIQKFMLRDQVKSAKAILE

Sequences:

>Translated_549_residues
MSTPQGHYSIGLDKTPANFVPLSPLSFLERTANVYPEMTSVVYEGRHYTWKETRARCRRFASWLTRSGIGRGDTVAVMLP
NVPAMVEVHFAVPMAGAVLNALNIRLDAAAIAFQLDHGGAKIILVDPEFSSVVAEALKLMTKPKPLVIDVDDKMYPGSHR
IGELEYEYAVASGDPTFAGHRPEEEWDAISLGYTSGTTGNPKGVVTHHRGAYLNAVSNILAGNLGQHPVYLWTLPMFHCN
GWCFPWTLAAAAGINVCLRKVDPAKIFELIPKHGVTHMCGAPIVYNALINAPEAPKGAATKPVVGLIAGAAPPMAVLAGA
ETIGIKLTHVYGLTEVYGPASVCAEQPGWDELPVAERAKLKRRQGVPYPMQEAVTVLNPETMQEVPRDGETIGEVMFRGN
IVMKGYLKNEKATKEALAGGWFHTGDLGVLDADGYVIIKDRSKDIIISGGENVSSVEVEDVLYKHPAILFAAVVAKPDPK
WGEVPCAFVELKDGASATEAEIIAYCREHLPGFKTPKSIVFSAIPKTSTGKIQKFMLRDQVKSAKAILE
>Mature_548_residues
STPQGHYSIGLDKTPANFVPLSPLSFLERTANVYPEMTSVVYEGRHYTWKETRARCRRFASWLTRSGIGRGDTVAVMLPN
VPAMVEVHFAVPMAGAVLNALNIRLDAAAIAFQLDHGGAKIILVDPEFSSVVAEALKLMTKPKPLVIDVDDKMYPGSHRI
GELEYEYAVASGDPTFAGHRPEEEWDAISLGYTSGTTGNPKGVVTHHRGAYLNAVSNILAGNLGQHPVYLWTLPMFHCNG
WCFPWTLAAAAGINVCLRKVDPAKIFELIPKHGVTHMCGAPIVYNALINAPEAPKGAATKPVVGLIAGAAPPMAVLAGAE
TIGIKLTHVYGLTEVYGPASVCAEQPGWDELPVAERAKLKRRQGVPYPMQEAVTVLNPETMQEVPRDGETIGEVMFRGNI
VMKGYLKNEKATKEALAGGWFHTGDLGVLDADGYVIIKDRSKDIIISGGENVSSVEVEDVLYKHPAILFAAVVAKPDPKW
GEVPCAFVELKDGASATEAEIIAYCREHLPGFKTPKSIVFSAIPKTSTGKIQKFMLRDQVKSAKAILE

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=590, Percent_Identity=30.1694915254237, Blast_Score=189, Evalue=8e-48,
Organism=Homo sapiens, GI122937307, Length=540, Percent_Identity=27.962962962963, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI157311624, Length=543, Percent_Identity=27.6243093922652, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI157311622, Length=543, Percent_Identity=27.6243093922652, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI58082049, Length=536, Percent_Identity=27.2388059701493, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI187761345, Length=581, Percent_Identity=25.9896729776248, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI187761343, Length=581, Percent_Identity=25.9896729776248, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI38505220, Length=508, Percent_Identity=28.1496062992126, Blast_Score=123, Evalue=6e-28,
Organism=Homo sapiens, GI42544132, Length=519, Percent_Identity=26.2042389210019, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI115511026, Length=521, Percent_Identity=26.295585412668, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI13375727, Length=542, Percent_Identity=24.7232472324723, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI27477105, Length=500, Percent_Identity=24.4, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI227499619, Length=541, Percent_Identity=23.8447319778189, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI253970489, Length=123, Percent_Identity=37.3983739837398, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI8923896, Length=123, Percent_Identity=37.3983739837398, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1788107, Length=553, Percent_Identity=27.8481012658228, Blast_Score=172, Evalue=6e-44,
Organism=Escherichia coli, GI145693145, Length=534, Percent_Identity=29.4007490636704, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI221142682, Length=526, Percent_Identity=25.4752851711027, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1786810, Length=545, Percent_Identity=25.3211009174312, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1788595, Length=518, Percent_Identity=24.9034749034749, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1786529, Length=571, Percent_Identity=25.0437828371278, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71994694, Length=538, Percent_Identity=28.4386617100372, Blast_Score=191, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI71994690, Length=538, Percent_Identity=28.4386617100372, Blast_Score=191, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI71994703, Length=538, Percent_Identity=28.4386617100372, Blast_Score=191, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17560308, Length=541, Percent_Identity=27.1719038817006, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17558820, Length=524, Percent_Identity=27.6717557251908, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17559526, Length=518, Percent_Identity=27.027027027027, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32563687, Length=508, Percent_Identity=25.7874015748031, Blast_Score=125, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17538037, Length=521, Percent_Identity=25.9117082533589, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17557194, Length=519, Percent_Identity=26.0115606936416, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17531443, Length=517, Percent_Identity=23.4042553191489, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17560140, Length=501, Percent_Identity=22.9540918163673, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71996755, Length=527, Percent_Identity=24.4781783681214, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71983001, Length=562, Percent_Identity=24.0213523131673, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71982997, Length=562, Percent_Identity=24.0213523131673, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71985884, Length=359, Percent_Identity=30.6406685236769, Blast_Score=90, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6319699, Length=499, Percent_Identity=25.8517034068136, Blast_Score=116, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6319264, Length=587, Percent_Identity=22.4872231686542, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323182, Length=135, Percent_Identity=34.0740740740741, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24581924, Length=568, Percent_Identity=26.5845070422535, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI18859661, Length=521, Percent_Identity=27.6391554702495, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI21355181, Length=520, Percent_Identity=27.1153846153846, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI21358303, Length=513, Percent_Identity=23.5867446393762, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648255, Length=529, Percent_Identity=23.2514177693762, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281365686, Length=515, Percent_Identity=23.3009708737864, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19922652, Length=510, Percent_Identity=24.9019607843137, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356947, Length=523, Percent_Identity=23.7093690248566, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648260, Length=539, Percent_Identity=23.3766233766234, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21356441, Length=475, Percent_Identity=23.7894736842105, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24667955, Length=551, Percent_Identity=23.7749546279492, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI62472339, Length=551, Percent_Identity=23.7749546279492, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24667959, Length=380, Percent_Identity=26.0526315789474, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI20130357, Length=450, Percent_Identity=22.4444444444444, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI19921316, Length=498, Percent_Identity=23.8955823293173, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17933690, Length=163, Percent_Identity=30.0613496932515, Blast_Score=70, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 59387; Mature: 59256

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPQGHYSIGLDKTPANFVPLSPLSFLERTANVYPEMTSVVYEGRHYTWKETRARCRRF
CCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHCCCCCCCHHHHHHHHHHH
ASWLTRSGIGRGDTVAVMLPNVPAMVEVHFAVPMAGAVLNALNIRLDAAAIAFQLDHGGA
HHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHEEEEEEEEEEEEECCCE
KIILVDPEFSSVVAEALKLMTKPKPLVIDVDDKMYPGSHRIGELEYEYAVASGDPTFAGH
EEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCC
RPEEEWDAISLGYTSGTTGNPKGVVTHHRGAYLNAVSNILAGNLGQHPVYLWTLPMFHCN
CCCCCCCEEEEECCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCEEEEEECHHHCC
GWCFPWTLAAAAGINVCLRKVDPAKIFELIPKHGVTHMCGAPIVYNALINAPEAPKGAAT
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHCCCCHHHHHHHCCCCCCCCCCC
KPVVGLIAGAAPPMAVLAGAETIGIKLTHVYGLTEVYGPASVCAEQPGWDELPVAERAKL
CCHHHHHHCCCCCHHHHCCCCEEEEEEEEEECHHHHCCCHHHHCCCCCCCCCCHHHHHHH
KRRQGVPYPMQEAVTVLNPETMQEVPRDGETIGEVMFRGNIVMKGYLKNEKATKEALAGG
HHHCCCCCCHHHHHHHCCCHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHCC
WFHTGDLGVLDADGYVIIKDRSKDIIISGGENVSSVEVEDVLYKHPAILFAAVVAKPDPK
CEECCCEEEEECCCEEEEEECCCEEEEECCCCCCEEEHHHHHHHCHHHHHHHHHCCCCCC
WGEVPCAFVELKDGASATEAEIIAYCREHLPGFKTPKSIVFSAIPKTSTGKIQKFMLRDQ
CCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHH
VKSAKAILE
HHHHHHHCC
>Mature Secondary Structure 
STPQGHYSIGLDKTPANFVPLSPLSFLERTANVYPEMTSVVYEGRHYTWKETRARCRRF
CCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHCCCCCCCHHHHHHHHHHH
ASWLTRSGIGRGDTVAVMLPNVPAMVEVHFAVPMAGAVLNALNIRLDAAAIAFQLDHGGA
HHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHEEEEEEEEEEEEECCCE
KIILVDPEFSSVVAEALKLMTKPKPLVIDVDDKMYPGSHRIGELEYEYAVASGDPTFAGH
EEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCC
RPEEEWDAISLGYTSGTTGNPKGVVTHHRGAYLNAVSNILAGNLGQHPVYLWTLPMFHCN
CCCCCCCEEEEECCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCEEEEEECHHHCC
GWCFPWTLAAAAGINVCLRKVDPAKIFELIPKHGVTHMCGAPIVYNALINAPEAPKGAAT
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHCCCCHHHHHHHCCCCCCCCCCC
KPVVGLIAGAAPPMAVLAGAETIGIKLTHVYGLTEVYGPASVCAEQPGWDELPVAERAKL
CCHHHHHHCCCCCHHHHCCCCEEEEEEEEEECHHHHCCCHHHHCCCCCCCCCCHHHHHHH
KRRQGVPYPMQEAVTVLNPETMQEVPRDGETIGEVMFRGNIVMKGYLKNEKATKEALAGG
HHHCCCCCCHHHHHHHCCCHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHCC
WFHTGDLGVLDADGYVIIKDRSKDIIISGGENVSSVEVEDVLYKHPAILFAAVVAKPDPK
CEECCCEEEEECCCEEEEEECCCEEEEECCCCCCEEEHHHHHHHCHHHHHHHHHCCCCCC
WGEVPCAFVELKDGASATEAEIIAYCREHLPGFKTPKSIVFSAIPKTSTGKIQKFMLRDQ
CCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHH
VKSAKAILE
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]