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The map label for this gene is yfhM [H]

Identifier: 192291488

GI number: 192291488

Start: 3355434

End: 3356381

Strand: Reverse

Name: yfhM [H]

Synonym: Rpal_3112

Alternate gene names: 192291488

Gene position: 3356381-3355434 (Counterclockwise)

Preceding gene: 192291495

Following gene: 192291486

Centisome position: 58.43

GC content: 64.35

Gene sequence:

>948_bases
ATGGCTTCCACACGCACGATCACGGCCAACGGCATCGAATTGTTCCTGCGCGAACAGGGCGAAGGTCCGCTCGTGGTCTT
GTGTCACGGCTGGCCGGAACTGTCATACTCGTGGCGGCATCAGATCGGCGCGCTGGCGGACGCCGGTTATCATGTGGTGG
CGCCGGACATGCGCGGTTTCGGCCGCTCCTCGGCGCCGCAGGCGGTCGAAGCCTACAGCATCTTCGATCTGGTCGGCGAT
ATGGTGGCGTTGGTGGCCGAGCTCGGCGAGACCCGTGCTGCGATCATCGGCCATGATTGGGGCGCACCGGTCGCCTGGCA
CGCGGCCCAGTTCAGGCCAGATCTGTTCGCCGCCGTGGCGGGGCTCAGCGTGCCGCCGCCATGGCGCGGCAAAGGCCCGC
CGCTGGATCAGCTGCGCGCCGCCGGAATCACCAACTTCTATTGGCAGTATTTCCAGAAGCCTGGCGTCGCCGAGACCGAG
TTCGAGCGCGACGTCGCCAGTACGATGCGCGGCATTTTGTGCGGCGGCTTCGCCGACCCGGGGCGCTCGCTGTTCGTTCC
GGAGGGGCGCGGCTTCATCGGCCGCTCGGCGGCATCGCTTCCGTTGCCGCCCTGGCTGTCCGAGGCAGAGCTCGCCTTCT
TCATCGAACAATACAAACAGAGCGGTTTCCGCGGCGGCCTCAACTGGTACCGGAACATCGACCGCAACTGGGAACTGACG
TCGCCGTGGCAAGGCGCGCCCATTCACCAGCCAGCGGCGTTCATTGCCGGCAGCAACGATCCGGTGATCTCGGACAAAAT
GAGCGGCAAACATCTGGCCGCGATCAACCGGGTGCTGCCCAACCTCAAGCAGAAGCTGATTATCGATGGTGCCGGACACT
GGATCCAGCAGGAGAAGCCCGCCGAAGTGAATGCGGCGTTGATCGAATTTCTGAAGGAGAATTACTGA

Upstream 100 bases:

>100_bases
CGGGGCGGCCGTTTTCGATTGTGGTGACGAACGATCGATCCTACCGTGAGCGCAAGCTCGCTTCCAGACAAACTCAAGCA
ACAAACAGGGCGGATACACC

Downstream 100 bases:

>100_bases
GGCGGTTGCAGCGGCTGGAGCGAGCCGTTCAGTAAAGGCCGCGGGCCGACAGCAGGTTGCCGATCTCATCGTTGCGCCGG
ACCGGCGCACTTGCCGGCTC

Product: alpha/beta hydrolase fold protein

Products: glycol

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MASTRTITANGIELFLREQGEGPLVVLCHGWPELSYSWRHQIGALADAGYHVVAPDMRGFGRSSAPQAVEAYSIFDLVGD
MVALVAELGETRAAIIGHDWGAPVAWHAAQFRPDLFAAVAGLSVPPPWRGKGPPLDQLRAAGITNFYWQYFQKPGVAETE
FERDVASTMRGILCGGFADPGRSLFVPEGRGFIGRSAASLPLPPWLSEAELAFFIEQYKQSGFRGGLNWYRNIDRNWELT
SPWQGAPIHQPAAFIAGSNDPVISDKMSGKHLAAINRVLPNLKQKLIIDGAGHWIQQEKPAEVNAALIEFLKENY

Sequences:

>Translated_315_residues
MASTRTITANGIELFLREQGEGPLVVLCHGWPELSYSWRHQIGALADAGYHVVAPDMRGFGRSSAPQAVEAYSIFDLVGD
MVALVAELGETRAAIIGHDWGAPVAWHAAQFRPDLFAAVAGLSVPPPWRGKGPPLDQLRAAGITNFYWQYFQKPGVAETE
FERDVASTMRGILCGGFADPGRSLFVPEGRGFIGRSAASLPLPPWLSEAELAFFIEQYKQSGFRGGLNWYRNIDRNWELT
SPWQGAPIHQPAAFIAGSNDPVISDKMSGKHLAAINRVLPNLKQKLIIDGAGHWIQQEKPAEVNAALIEFLKENY
>Mature_314_residues
ASTRTITANGIELFLREQGEGPLVVLCHGWPELSYSWRHQIGALADAGYHVVAPDMRGFGRSSAPQAVEAYSIFDLVGDM
VALVAELGETRAAIIGHDWGAPVAWHAAQFRPDLFAAVAGLSVPPPWRGKGPPLDQLRAAGITNFYWQYFQKPGVAETEF
ERDVASTMRGILCGGFADPGRSLFVPEGRGFIGRSAASLPLPPWLSEAELAFFIEQYKQSGFRGGLNWYRNIDRNWELTS
PWQGAPIHQPAAFIAGSNDPVISDKMSGKHLAAINRVLPNLKQKLIIDGAGHWIQQEKPAEVNAALIEFLKENY

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI27597073, Length=304, Percent_Identity=40.4605263157895, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI218777837, Length=299, Percent_Identity=29.0969899665552, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI218777840, Length=296, Percent_Identity=28.3783783783784, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI13376164, Length=296, Percent_Identity=28.3783783783784, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17554012, Length=320, Percent_Identity=28.125, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17562298, Length=314, Percent_Identity=22.9299363057325, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.3.2.3

Molecular weight: Translated: 34547; Mature: 34415

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASTRTITANGIELFLREQGEGPLVVLCHGWPELSYSWRHQIGALADAGYHVVAPDMRGF
CCCCCEEECCCEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEECCCHHCC
GRSSAPQAVEAYSIFDLVGDMVALVAELGETRAAIIGHDWGAPVAWHAAQFRPDLFAAVA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCHHHHHHHH
GLSVPPPWRGKGPPLDQLRAAGITNFYWQYFQKPGVAETEFERDVASTMRGILCGGFADP
CCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
GRSLFVPEGRGFIGRSAASLPLPPWLSEAELAFFIEQYKQSGFRGGLNWYRNIDRNWELT
CCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCC
SPWQGAPIHQPAAFIAGSNDPVISDKMSGKHLAAINRVLPNLKQKLIIDGAGHWIQQEKP
CCCCCCCCCCCCEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCC
AEVNAALIEFLKENY
CHHHHHHHHHHHHCC
>Mature Secondary Structure 
ASTRTITANGIELFLREQGEGPLVVLCHGWPELSYSWRHQIGALADAGYHVVAPDMRGF
CCCCEEECCCEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEECCCHHCC
GRSSAPQAVEAYSIFDLVGDMVALVAELGETRAAIIGHDWGAPVAWHAAQFRPDLFAAVA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCHHHHHHHH
GLSVPPPWRGKGPPLDQLRAAGITNFYWQYFQKPGVAETEFERDVASTMRGILCGGFADP
CCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
GRSLFVPEGRGFIGRSAASLPLPPWLSEAELAFFIEQYKQSGFRGGLNWYRNIDRNWELT
CCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCC
SPWQGAPIHQPAAFIAGSNDPVISDKMSGKHLAAINRVLPNLKQKLIIDGAGHWIQQEKP
CCCCCCCCCCCCEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCC
AEVNAALIEFLKENY
CHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; epoxide

Specific reaction: H2O + an epoxide = a glycol

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]