BacMap

Definition	Rhodopseudomonas palustris TIE-1 chromosome, complete genome.
Accession	NC_011004
Length	5,744,041

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The map label for this gene is paaF [C]

Identifier: 192291480

GI number: 192291480

Start: 3348720

End: 3349547

Strand: Reverse

Name: paaF [C]

Synonym: Rpal_3104

Alternate gene names: 192291480

Gene position: 3349547-3348720 (Counterclockwise)

Preceding gene: 192291486

Following gene: 192291479

Centisome position: 58.31

GC content: 66.3

Gene sequence:

>828_bases
ATGACCGTGTTGCCCGCTTTCGCAGCGACCCAGCCTTCACCGATTCTGCTGCGTGAGACCGTCGATGGCGGTGTTGCAGT
GCTGACGTTGAACAGACCGGCCGCGCGCAACAGCCTGTCGCTGGAAATGATCGACGATCTGCACGCGGCGCTGGATCTGA
TCGCGCGCGACGATGGCGTGCGTGTCGTGGTTATCGCCGCCAATGGCCCGGCGTTCTGCGCCGGCCATGACCTCAAAGAG
CTGACCGCGCATCGCAATGACGCCGATCGCGGCCGTGCCAGCTTCACCCGGATCATGACCGCCTGCAGTGCGATGATGCA
GGCGATCGTCAAGCTGCCCAAGCCGGTGATCGCGGCGGTGCAGGGCGTCGCCACCGCGGCGGGCTGTCAGCTGGTCGCGA
GCTGCGACCTCGCGATCGCATCCGAGCAGGCGACCTTCGCGACGCCCGGCGTCGATATCGGACTGTTCTGCTCCACCCCG
ATGGTGGCGCTGTCGCGCAACGTGCCGCGCAAGAACGCGATGCACATGCTACTGACCGGCGAGCCGGTCGGCGCCGAAGA
GGCCCGCCAGATCGGCCTGATCAACCGCGTGGTGCCGCACGGCACCGAGCGCGCCGCGGCCGTCGCACTGGCCGAGCAGA
TCGCCGGCAAGTCGGCCCACACCATCAAGATCGGCAAGGAGGCGTTCTACCGCCAGGCCGAGCTCAACCTCGCCGACGCC
TATCACTACGCCTCGCAGGTGATGGCCGAGAACATGATGGCCGCCGATGCCGAAGAAGGCATTTGTGCCTTCCTGGAAAA
GCGCCAGCCGGAATGGAAGGATCGTTGA

Upstream 100 bases:

>100_bases
CATGTGTCATGTCGTTCCCGCCCCTGCTCTCGCTTCGTTCGGCTTGTTACAATAAGGTGACGCTCGTTCCCAGCGCTTCA
TTTTGTTCCCGGACGTCGCC

Downstream 100 bases:

>100_bases
TCCGCGGCGTCGACCATGCCCCGTCATTGCGAGCGAAGCGAAGCAATCCAGGGCCAAGCACCGGAAGCTGGATTGCTTCG
TCGCGGAGCCTGTGCTCGGA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTVLPAFAATQPSPILLRETVDGGVAVLTLNRPAARNSLSLEMIDDLHAALDLIARDDGVRVVVIAANGPAFCAGHDLKE
LTAHRNDADRGRASFTRIMTACSAMMQAIVKLPKPVIAAVQGVATAAGCQLVASCDLAIASEQATFATPGVDIGLFCSTP
MVALSRNVPRKNAMHMLLTGEPVGAEEARQIGLINRVVPHGTERAAAVALAEQIAGKSAHTIKIGKEAFYRQAELNLADA
YHYASQVMAENMMAADAEEGICAFLEKRQPEWKDR

Sequences:

>Translated_275_residues
MTVLPAFAATQPSPILLRETVDGGVAVLTLNRPAARNSLSLEMIDDLHAALDLIARDDGVRVVVIAANGPAFCAGHDLKE
LTAHRNDADRGRASFTRIMTACSAMMQAIVKLPKPVIAAVQGVATAAGCQLVASCDLAIASEQATFATPGVDIGLFCSTP
MVALSRNVPRKNAMHMLLTGEPVGAEEARQIGLINRVVPHGTERAAAVALAEQIAGKSAHTIKIGKEAFYRQAELNLADA
YHYASQVMAENMMAADAEEGICAFLEKRQPEWKDR
>Mature_274_residues
TVLPAFAATQPSPILLRETVDGGVAVLTLNRPAARNSLSLEMIDDLHAALDLIARDDGVRVVVIAANGPAFCAGHDLKEL
TAHRNDADRGRASFTRIMTACSAMMQAIVKLPKPVIAAVQGVATAAGCQLVASCDLAIASEQATFATPGVDIGLFCSTPM
VALSRNVPRKNAMHMLLTGEPVGAEEARQIGLINRVVPHGTERAAAVALAEQIAGKSAHTIKIGKEAFYRQAELNLADAY
HYASQVMAENMMAADAEEGICAFLEKRQPEWKDR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=263, Percent_Identity=45.6273764258555, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI194097323, Length=255, Percent_Identity=32.9411764705882, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4502327, Length=267, Percent_Identity=28.0898876404494, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI221136756, Length=261, Percent_Identity=26.8199233716475, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI221136753, Length=261, Percent_Identity=26.8199233716475, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI4757968, Length=268, Percent_Identity=26.865671641791, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI49355787, Length=268, Percent_Identity=26.865671641791, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI221307494, Length=261, Percent_Identity=26.8199233716475, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI260274832, Length=257, Percent_Identity=24.5136186770428, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI45643119, Length=257, Percent_Identity=24.5136186770428, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI260275230, Length=257, Percent_Identity=24.5136186770428, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI25453481, Length=255, Percent_Identity=25.8823529411765, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI51538573, Length=255, Percent_Identity=25.8823529411765, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI4757966, Length=185, Percent_Identity=30.2702702702703, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI51538581, Length=185, Percent_Identity=30.2702702702703, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI70995211, Length=179, Percent_Identity=30.7262569832402, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI116235487, Length=181, Percent_Identity=31.4917127071823, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI37594469, Length=181, Percent_Identity=28.1767955801105, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI37594471, Length=181, Percent_Identity=28.1767955801105, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI20127408, Length=186, Percent_Identity=26.8817204301075, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787659, Length=252, Percent_Identity=35.3174603174603, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI221142681, Length=270, Percent_Identity=33.3333333333333, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=35.1145038167939, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87082183, Length=253, Percent_Identity=28.0632411067194, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1788597, Length=257, Percent_Identity=27.2373540856031, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790281, Length=187, Percent_Identity=24.5989304812834, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788682, Length=201, Percent_Identity=27.8606965174129, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=271, Percent_Identity=44.280442804428, Blast_Score=214, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI17554946, Length=272, Percent_Identity=33.0882352941176, Blast_Score=143, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI25145438, Length=252, Percent_Identity=32.9365079365079, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17560910, Length=264, Percent_Identity=29.5454545454545, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17535521, Length=259, Percent_Identity=27.027027027027, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=262, Percent_Identity=25.5725190839695, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17540714, Length=231, Percent_Identity=27.7056277056277, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=212, Percent_Identity=31.6037735849057, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=253, Percent_Identity=28.0632411067194, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25144160, Length=182, Percent_Identity=28.021978021978, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25144157, Length=182, Percent_Identity=28.021978021978, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17558304, Length=222, Percent_Identity=24.3243243243243, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=202, Percent_Identity=27.2277227722772, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19922422, Length=270, Percent_Identity=39.2592592592593, Blast_Score=188, Evalue=3e-48,
Organism=Drosophila melanogaster, GI20129971, Length=251, Percent_Identity=31.8725099601594, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24653477, Length=251, Percent_Identity=31.8725099601594, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24653139, Length=270, Percent_Identity=31.4814814814815, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21357171, Length=273, Percent_Identity=27.4725274725275, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24650670, Length=281, Percent_Identity=29.1814946619217, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI19920382, Length=241, Percent_Identity=27.8008298755187, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24654903, Length=179, Percent_Identity=30.7262569832402, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583165, Length=181, Percent_Identity=29.2817679558011, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19921018, Length=181, Percent_Identity=24.8618784530387, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29195; Mature: 29064

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLPAFAATQPSPILLRETVDGGVAVLTLNRPAARNSLSLEMIDDLHAALDLIARDDGV
CCCCCCHHCCCCCCEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
RVVVIAANGPAFCAGHDLKELTAHRNDADRGRASFTRIMTACSAMMQAIVKLPKPVIAAV
EEEEEECCCCCEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
QGVATAAGCQLVASCDLAIASEQATFATPGVDIGLFCSTPMVALSRNVPRKNAMHMLLTG
HHHHHHHHHHHEECCCEEEECCCCEEECCCCCEEEECCCCHHHHHCCCCCCCCEEEEEEC
EPVGAEEARQIGLINRVVPHGTERAAAVALAEQIAGKSAHTIKIGKEAFYRQAELNLADA
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHCCHHHH
YHYASQVMAENMMAADAEEGICAFLEKRQPEWKDR
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TVLPAFAATQPSPILLRETVDGGVAVLTLNRPAARNSLSLEMIDDLHAALDLIARDDGV
CCCCCHHCCCCCCEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
RVVVIAANGPAFCAGHDLKELTAHRNDADRGRASFTRIMTACSAMMQAIVKLPKPVIAAV
EEEEEECCCCCEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
QGVATAAGCQLVASCDLAIASEQATFATPGVDIGLFCSTPMVALSRNVPRKNAMHMLLTG
HHHHHHHHHHHEECCCEEEECCCCEEECCCCCEEEECCCCHHHHHCCCCCCCCEEEEEEC
EPVGAEEARQIGLINRVVPHGTERAAAVALAEQIAGKSAHTIKIGKEAFYRQAELNLADA
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHCCHHHH
YHYASQVMAENMMAADAEEGICAFLEKRQPEWKDR
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA