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The map label for this gene is kipI [H]

Identifier: 192291336

GI number: 192291336

Start: 3231020

End: 3231748

Strand: Reverse

Name: kipI [H]

Synonym: Rpal_2958

Alternate gene names: 192291336

Gene position: 3231748-3231020 (Counterclockwise)

Preceding gene: 192291343

Following gene: 192291335

Centisome position: 56.26

GC content: 63.1

Gene sequence:

>729_bases
ATGACCACCCCACCTCCCAGGATGCTGCCGAGCGGTGACACAGCGATCACCGTCGAGTTCGGCCGAGATATCAGCGATGA
ATCCAATCGCCGCGTGCTGGCGCTCGATCGCAAGCTGAAGCAAGCCCCGATCGCCGGTATCCTCGAAACCGTGCCGACCT
ATCGTTCGCTTCTAGTGCATTACGATCCGGAGACGATCGGCTTTGGCGCGCTGTCCGATCAGCTCACAGCGCTCGCACAT
ACATCCGACGAAAGCGAAACTGTCGCCAAACGGCGCTGGCGGATTCCGGTGTGTTATGGCGGCGAGCACGGCATCGATCT
GGAAGACGCCGCGCAGGCGTTGAACATCACGCCGGACGCGCTGGTCGCGCGCCACGCCGCTGGCGATTATCGCGTCGCCA
TGATCGGCTTCACACCGGGCTGGTCATATCTTTCCGGCCTCGATCCGGCCTTGGCGATGCCACGACGGCAAGCGCCGCGA
CTGCACACCCCGGCCGGGACGATTTCGGTCGGCGGCATTCAGACCGGCATCCAATGTCTGGCCGGACCGAGCGGATGGCA
CCTGCTCGGCCGCACGCCGGTGCGGACTTATCAGTTGCATCGCGAGCCGATCTTTCTGCTCGAACCCGGCGACGCAATCA
GCTTCGCGCAGATCGATGCCAAGGAATTCGCCGAACGAGACGATGCAGCGTCTCATGGTGAAGTCGTCGCGGAACTGCTG
AGCGCATGA

Upstream 100 bases:

>100_bases
AGGAGCATCGACTTATCGGCCTCGCGTTAGTTGCGAAGTGCTTTTCAGCCGCTCGGAAAAGGCGCTATCGCTGCCGGTAG
CTTTGTGAGATCGTTGCCGC

Downstream 100 bases:

>100_bases
CGAAGCTTGTAATCGACGCCGTCGGACCGGCCACCTCCGTGCAGGACGCCGGGCGCCACGGCGCACAGCGTTACGGCCTC
CCGCCGAGCGGCGCGATGGA

Product: Allophanate hydrolase subunit 1

Products: NA

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MTTPPPRMLPSGDTAITVEFGRDISDESNRRVLALDRKLKQAPIAGILETVPTYRSLLVHYDPETIGFGALSDQLTALAH
TSDESETVAKRRWRIPVCYGGEHGIDLEDAAQALNITPDALVARHAAGDYRVAMIGFTPGWSYLSGLDPALAMPRRQAPR
LHTPAGTISVGGIQTGIQCLAGPSGWHLLGRTPVRTYQLHREPIFLLEPGDAISFAQIDAKEFAERDDAASHGEVVAELL
SA

Sequences:

>Translated_242_residues
MTTPPPRMLPSGDTAITVEFGRDISDESNRRVLALDRKLKQAPIAGILETVPTYRSLLVHYDPETIGFGALSDQLTALAH
TSDESETVAKRRWRIPVCYGGEHGIDLEDAAQALNITPDALVARHAAGDYRVAMIGFTPGWSYLSGLDPALAMPRRQAPR
LHTPAGTISVGGIQTGIQCLAGPSGWHLLGRTPVRTYQLHREPIFLLEPGDAISFAQIDAKEFAERDDAASHGEVVAELL
SA
>Mature_241_residues
TTPPPRMLPSGDTAITVEFGRDISDESNRRVLALDRKLKQAPIAGILETVPTYRSLLVHYDPETIGFGALSDQLTALAHT
SDESETVAKRRWRIPVCYGGEHGIDLEDAAQALNITPDALVARHAAGDYRVAMIGFTPGWSYLSGLDPALAMPRRQAPRL
HTPAGTISVGGIQTGIQCLAGPSGWHLLGRTPVRTYQLHREPIFLLEPGDAISFAQIDAKEFAERDDAASHGEVVAELLS
A

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=208, Percent_Identity=33.6538461538462, Blast_Score=118, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: NA

Molecular weight: Translated: 26155; Mature: 26024

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPPPRMLPSGDTAITVEFGRDISDESNRRVLALDRKLKQAPIAGILETVPTYRSLLVH
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECHHHHHCCHHHHHHHHHHHHEEEEE
YDPETIGFGALSDQLTALAHTSDESETVAKRRWRIPVCYGGEHGIDLEDAAQALNITPDA
ECCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCHHH
LVARHAAGDYRVAMIGFTPGWSYLSGLDPALAMPRRQAPRLHTPAGTISVGGIQTGIQCL
HHHHHCCCCEEEEEEEECCCHHHHHCCCHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHH
AGPSGWHLLGRTPVRTYQLHREPIFLLEPGDAISFAQIDAKEFAERDDAASHGEVVAELL
CCCCCCEEECCCCCCEEEECCCCEEEECCCCCEEEEECCHHHHHHHCCCCHHHHHHHHHH
SA
CC
>Mature Secondary Structure 
TTPPPRMLPSGDTAITVEFGRDISDESNRRVLALDRKLKQAPIAGILETVPTYRSLLVH
CCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECHHHHHCCHHHHHHHHHHHHEEEEE
YDPETIGFGALSDQLTALAHTSDESETVAKRRWRIPVCYGGEHGIDLEDAAQALNITPDA
ECCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCHHH
LVARHAAGDYRVAMIGFTPGWSYLSGLDPALAMPRRQAPRLHTPAGTISVGGIQTGIQCL
HHHHHCCCCEEEEEEEECCCHHHHHCCCHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHH
AGPSGWHLLGRTPVRTYQLHREPIFLLEPGDAISFAQIDAKEFAERDDAASHGEVVAELL
CCCCCCEEECCCCCCEEEECCCCEEEECCCCCEEEEECCHHHHHHHCCCCHHHHHHHHHH
SA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]