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The map label for this gene is phbC [H]

Identifier: 192291159

GI number: 192291159

Start: 3022563

End: 3024368

Strand: Reverse

Name: phbC [H]

Synonym: Rpal_2780

Alternate gene names: 192291159

Gene position: 3024368-3022563 (Counterclockwise)

Preceding gene: 192291167

Following gene: 192291154

Centisome position: 52.65

GC content: 64.01

Gene sequence:

>1806_bases
ATGACCGACATGCTCGCAGAACCCCAGGCGGCCCCGAAGTTCGATCCTGACGCGTTCGCCCGCAATCTCGCGCAGGCGAT
GGAGACCGGTAGCCAGGCCTTAGCGACGATGATGAAGTCGCAAAACGGTGCCGTGCCACCGGACGGTCATCCGCCCGCCG
GTCTGACCGAAATGGTGAAGAGCTTCTCGAGCGTCGCCAATTACTGGCTATCCGACCAGGCCCGTTCTCATGAGCTGCAG
CAGCGGCTCGGCAAGTCGTATCTCGAACTCTGGGGCGCGGCCTCCAAGCGCCTCGCCGGCGAACAGGCCGAGCCGGCGAT
CGCCCCCTCGCCGCGCGACAAGCGGTTCGCCTCACCGGAATGGAAGGCCAACGCGTTCTACGATTTCCTGATGCAGGCCT
ACCTGCTGACGACGCAGTGGGCCGACGAACTGGTCAAGAACGCCGAGATCGACCCGCACACCAAGCGCAAGGCCGCGTTC
TACGTCCAGCAGCTCACCACCGCTTTGGCGCCCTCCAACTTCGTGATGACCAATCCGGAGCTGACGCGCCAGACGCTGGA
GGCCAGCGGCGACAATCTGGTGCGCGGCATGAAGATGCTGGCCGACGACATCCAGTCAGGCCGCGGCCATCTGAAGATCC
GGCAGTCCGATCCGGCCGGGCTCGAGGTCGGCGTCAACATGGCGGTCACCCCGGGCAAGGTGATCTTCCAGAACGAAATC
ATGCAGCTCATCCAGTATGAGCCCGCCACCGCGACGGTGCAGCGCACGCCGCTGCTGATCGTGCCGCCGTGGATCAACAA
GTACTACATTCTCGATCTGAAGCCCGAGAAGTCGTTCATCAAATGGTGCGTCGACCAGGGCCTCACCGTGTTCGTGATCT
CCTGGGTCAACCCGGACAAGAGCCTCGCCGACAAGGACTTCGCCGACTACATGAAGCTCGGCCCGCTGACCGCGATGGAC
GTCGTCGAGAAGGTCACCGGCGAGATGAAGGTCCACACGCTGGGCTACTGCGTCGGCGGCACCCTGCTCGCCTCGACGCT
GGCCTGGCTCGCCGAGCGCCGCCGGGTCCGCGTTACCTCGGCGACCTTCCTGACCACGCAGGTCGACTTCACCCATGCCG
GCGACCTGATGGTGTTCGTCGACGAGGAGCAGATTTCCGCGGTCGAACGCGAGATGAAGGTCACCGGCGTGCTCGAAGGC
GCCAAGATGGCGATGGCCTTCAACATGCTGCGGCCGAACGATCTGATCTGGTCCTACGTCGTCAATAACTACCTGAAGGG
CCAGCCGCCGCAGGCGTTCGACCTGCTGCACTGGAATTCCGACGCCACCCGGATGCCGGCGGCGAACCACTCCTACTACC
TGCGCAACTGCTATCTCGACAACAAGCTGTCGGCCGGCACCATGGTGCTGGACAACACCACGCTCGACCTCACCAAGGTC
AAGGTGCCGATCTACAACCTCGCCACCCGCGAAGATCACATCGCGCCGGCGGAGTCGGTGCTGTACGGCTCGCAGTTCTT
CGGCGGTCCGGTGAAATTCGTGCTGTCCGGCTCCGGCCACATCGCCGGCGTGATCAATCCGCCGGTGGCGAACAAGTACC
TATTCTGGACCAACCCCGACATCTTCGCCGGCTCGGTCGCCAATTGGCTGAACGGCGCCGAAGAGCACAAGGGGTCGTGG
TGGCCGGATTGGCGCGCTTGGATCGGCCAGTTCGACGACGAACAGGTTCCCGCCCGCGCCATCGGCAACGAAGCCTACCC
GCCGCTGGAAGATGCGCCGGGCAGCTACGTCAAAGTGCGATCCTGA

Upstream 100 bases:

>100_bases
AGCACATTGAAATCAGCGACGTTTACCGAACTATGCCATCATCGGGGGGCGCGACGACAAATCTCAGCGGATTTTCTGCA
CCGGACTGTAGTGTTGATTC

Downstream 100 bases:

>100_bases
ACGACGCGCGGCGGCGAAGCAGCAGCCGCACGTCGCTCAAGTTAGTTCGCCAGCGCCGCCTCGACCACCCGCGCGGTGCG
CAGCAGCTTGGCGTCCTCGC

Product: poly(R)-hydroxyalkanoic acid synthase, class I

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 601; Mature: 600

Protein sequence:

>601_residues
MTDMLAEPQAAPKFDPDAFARNLAQAMETGSQALATMMKSQNGAVPPDGHPPAGLTEMVKSFSSVANYWLSDQARSHELQ
QRLGKSYLELWGAASKRLAGEQAEPAIAPSPRDKRFASPEWKANAFYDFLMQAYLLTTQWADELVKNAEIDPHTKRKAAF
YVQQLTTALAPSNFVMTNPELTRQTLEASGDNLVRGMKMLADDIQSGRGHLKIRQSDPAGLEVGVNMAVTPGKVIFQNEI
MQLIQYEPATATVQRTPLLIVPPWINKYYILDLKPEKSFIKWCVDQGLTVFVISWVNPDKSLADKDFADYMKLGPLTAMD
VVEKVTGEMKVHTLGYCVGGTLLASTLAWLAERRRVRVTSATFLTTQVDFTHAGDLMVFVDEEQISAVEREMKVTGVLEG
AKMAMAFNMLRPNDLIWSYVVNNYLKGQPPQAFDLLHWNSDATRMPAANHSYYLRNCYLDNKLSAGTMVLDNTTLDLTKV
KVPIYNLATREDHIAPAESVLYGSQFFGGPVKFVLSGSGHIAGVINPPVANKYLFWTNPDIFAGSVANWLNGAEEHKGSW
WPDWRAWIGQFDDEQVPARAIGNEAYPPLEDAPGSYVKVRS

Sequences:

>Translated_601_residues
MTDMLAEPQAAPKFDPDAFARNLAQAMETGSQALATMMKSQNGAVPPDGHPPAGLTEMVKSFSSVANYWLSDQARSHELQ
QRLGKSYLELWGAASKRLAGEQAEPAIAPSPRDKRFASPEWKANAFYDFLMQAYLLTTQWADELVKNAEIDPHTKRKAAF
YVQQLTTALAPSNFVMTNPELTRQTLEASGDNLVRGMKMLADDIQSGRGHLKIRQSDPAGLEVGVNMAVTPGKVIFQNEI
MQLIQYEPATATVQRTPLLIVPPWINKYYILDLKPEKSFIKWCVDQGLTVFVISWVNPDKSLADKDFADYMKLGPLTAMD
VVEKVTGEMKVHTLGYCVGGTLLASTLAWLAERRRVRVTSATFLTTQVDFTHAGDLMVFVDEEQISAVEREMKVTGVLEG
AKMAMAFNMLRPNDLIWSYVVNNYLKGQPPQAFDLLHWNSDATRMPAANHSYYLRNCYLDNKLSAGTMVLDNTTLDLTKV
KVPIYNLATREDHIAPAESVLYGSQFFGGPVKFVLSGSGHIAGVINPPVANKYLFWTNPDIFAGSVANWLNGAEEHKGSW
WPDWRAWIGQFDDEQVPARAIGNEAYPPLEDAPGSYVKVRS
>Mature_600_residues
TDMLAEPQAAPKFDPDAFARNLAQAMETGSQALATMMKSQNGAVPPDGHPPAGLTEMVKSFSSVANYWLSDQARSHELQQ
RLGKSYLELWGAASKRLAGEQAEPAIAPSPRDKRFASPEWKANAFYDFLMQAYLLTTQWADELVKNAEIDPHTKRKAAFY
VQQLTTALAPSNFVMTNPELTRQTLEASGDNLVRGMKMLADDIQSGRGHLKIRQSDPAGLEVGVNMAVTPGKVIFQNEIM
QLIQYEPATATVQRTPLLIVPPWINKYYILDLKPEKSFIKWCVDQGLTVFVISWVNPDKSLADKDFADYMKLGPLTAMDV
VEKVTGEMKVHTLGYCVGGTLLASTLAWLAERRRVRVTSATFLTTQVDFTHAGDLMVFVDEEQISAVEREMKVTGVLEGA
KMAMAFNMLRPNDLIWSYVVNNYLKGQPPQAFDLLHWNSDATRMPAANHSYYLRNCYLDNKLSAGTMVLDNTTLDLTKVK
VPIYNLATREDHIAPAESVLYGSQFFGGPVKFVLSGSGHIAGVINPPVANKYLFWTNPDIFAGSVANWLNGAEEHKGSWW
PDWRAWIGQFDDEQVPARAIGNEAYPPLEDAPGSYVKVRS

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 66795; Mature: 66664

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDMLAEPQAAPKFDPDAFARNLAQAMETGSQALATMMKSQNGAVPPDGHPPAGLTEMVK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
SFSSVANYWLSDQARSHELQQRLGKSYLELWGAASKRLAGEQAEPAIAPSPRDKRFASPE
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
WKANAFYDFLMQAYLLTTQWADELVKNAEIDPHTKRKAAFYVQQLTTALAPSNFVMTNPE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECHH
LTRQTLEASGDNLVRGMKMLADDIQSGRGHLKIRQSDPAGLEVGVNMAVTPGKVIFQNEI
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEECCEEEECCCHHHHHHHH
MQLIQYEPATATVQRTPLLIVPPWINKYYILDLKPEKSFIKWCVDQGLTVFVISWVNPDK
HHHHHCCCCCCEEECCCEEEECCCCCCEEEEEECCCHHHHHHHHHCCCEEEEEEECCCCH
SLADKDFADYMKLGPLTAMDVVEKVTGEMKVHTLGYCVGGTLLASTLAWLAERRRVRVTS
HHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEE
ATFLTTQVDFTHAGDLMVFVDEEQISAVEREMKVTGVLEGAKMAMAFNMLRPNDLIWSYV
EEEEEEEECEEECCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
VNNYLKGQPPQAFDLLHWNSDATRMPAANHSYYLRNCYLDNKLSAGTMVLDNTTLDLTKV
HHHHHCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCEEEEEEE
KVPIYNLATREDHIAPAESVLYGSQFFGGPVKFVLSGSGHIAGVINPPVANKYLFWTNPD
EEEEEECCCCCCCCCCHHHHHCCCHHCCCCEEEEEECCCCEEEEECCCCCCCEEEECCCC
IFAGSVANWLNGAEEHKGSWWPDWRAWIGQFDDEQVPARAIGNEAYPPLEDAPGSYVKVR
CHHHHHHHHHCCHHHCCCCCCCCHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEC
S
C
>Mature Secondary Structure 
TDMLAEPQAAPKFDPDAFARNLAQAMETGSQALATMMKSQNGAVPPDGHPPAGLTEMVK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
SFSSVANYWLSDQARSHELQQRLGKSYLELWGAASKRLAGEQAEPAIAPSPRDKRFASPE
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
WKANAFYDFLMQAYLLTTQWADELVKNAEIDPHTKRKAAFYVQQLTTALAPSNFVMTNPE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECHH
LTRQTLEASGDNLVRGMKMLADDIQSGRGHLKIRQSDPAGLEVGVNMAVTPGKVIFQNEI
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEECCEEEECCCHHHHHHHH
MQLIQYEPATATVQRTPLLIVPPWINKYYILDLKPEKSFIKWCVDQGLTVFVISWVNPDK
HHHHHCCCCCCEEECCCEEEECCCCCCEEEEEECCCHHHHHHHHHCCCEEEEEEECCCCH
SLADKDFADYMKLGPLTAMDVVEKVTGEMKVHTLGYCVGGTLLASTLAWLAERRRVRVTS
HHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEE
ATFLTTQVDFTHAGDLMVFVDEEQISAVEREMKVTGVLEGAKMAMAFNMLRPNDLIWSYV
EEEEEEEECEEECCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
VNNYLKGQPPQAFDLLHWNSDATRMPAANHSYYLRNCYLDNKLSAGTMVLDNTTLDLTKV
HHHHHCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCEEEEEEE
KVPIYNLATREDHIAPAESVLYGSQFFGGPVKFVLSGSGHIAGVINPPVANKYLFWTNPD
EEEEEECCCCCCCCCCHHHHHCCCHHCCCCEEEEEECCCCEEEEECCCCCCCEEEECCCC
IFAGSVANWLNGAEEHKGSWWPDWRAWIGQFDDEQVPARAIGNEAYPPLEDAPGSYVKVR
CHHHHHHHHHCCHHHCCCCCCCCHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748438 [H]