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The map label for this gene is pitA [H]

Identifier: 192291069

GI number: 192291069

Start: 2918441

End: 2920081

Strand: Reverse

Name: pitA [H]

Synonym: Rpal_2689

Alternate gene names: 192291069

Gene position: 2920081-2918441 (Counterclockwise)

Preceding gene: 192291073

Following gene: 192291062

Centisome position: 50.84

GC content: 63.62

Gene sequence:

>1641_bases
ATGGGGCGTAGCGTGACCGATTTCACCATCAATCAAGCAATCGACGACAATGGGCCTCTTCAGCCGGCCGGTCGGCCGAA
CCTGAACAAAGGCTTCAATCCGCTGACGATGATCCTGTTCTTCGGGGTCCTCGGTGTCGGCATTCTGTTCGTGGCTTACA
GCATCTATGTCGACGTCGAGGCAACCGGCGTTCGCGTCACGACGTATTTGCCTTATCTGCTGCTGCTGGTGGCGCTGCTG
ATCGCGCTCGGCTTCGAGTTCGTCAACGGCTTCCACGACACCGCCAATGCAGTCGCGACCGTGATCTACACCCACTCGCT
GCCGGCCGAAGTTGCGGTCGTATGGTCAGGGGTGTTCAACTTCCTCGGCGTGCTGACCTCCACCGGTGCGGTGGCTTTCG
GCATCGTCTCGCTGCTGCCGGTGGAATTGATCCTGCAGGTCGGATCGAGCGCCGGCTTCGCAATGGTGTTCGCGCTGCTG
ATCGCCGCAATCCTGTGGAATCTCGGGACCTGGTACTTCGGCCTCCCCGCCTCCAGCTCGCACACCTTGATCGGCTCGAT
CATCGGCGTCGGTGTCGCCAATGCGCTGATGCGCGGCCGCGACGGCACTTCGGGAGTGGACTGGGGCAAGGCGACCGAGA
TCGGCTATGCGCTGCTGCTGTCGCCGCTGGTCGGCTTCGTCTGCGCCGCGATCCTGCTCCTGCTGCTCAAACTGGTCGTG
CGCAATCCGAAGCTGTACTCTGCGCCGGAAGGCAACGCGCCGCCGCCGTGGTGGATCCGCGGCCTGCTGATCCTGACCTG
TACCGGCGTCAGCTTCGCGCACGGCTCCAACGACGGTCAGAAAGGCATGGGCCTGATCATGCTGATCCTGATCGGCACCG
TGCCGACCGCCTATGCGCTCAATCGTGCCCTGCCGGATTCCCAGGTCGCGCATTTCGAGCAGACCTCGACGGCCGCATCG
GCTGTGATCGCGGCAAAGGGCGCCGGCTACAGCATCATCGGCGATCCCCGCCCCGCGGTGACGCAATACGTGTCCCAGCA
CAAGATCAGCGAAGGCACCTACCCGTCGCTGGCGGTGCTGGTGAAGGATATCGGCGAGCAGGTGCAGCGCTACGGTTCGC
TGAGCAAAGTGCCGGCCGAGTCCGTCGGCAACACTCGCAACGACATGTATCTGGCGTCCGAGGCGATCCGCTTCCTGATG
AAGGATAAGGAAAGCGACCTCAGCAAGGACGATGTTGCCAAGCTCAACGCCTACAAGGGCTCGCTCGACAGCGCGACGAA
GTTCATTCCGAACTGGGTCAAGATCGCAGTCGCGATCGCGCTCGGCCTCGGCACCATGATCGGCTGGAAGCGCATCGTCA
TCACCGTCGGTGAGAAGATCGGCAAGTCACATCTGACCTACGCGCAAGGCGCCTCCGCCGAGCTGGTGGCGGCCTCGACC
ATCGGCGCGGCGGACATGTTCGGCCTGCCGGTCTCGACCACCCACGTGCTGTCGTCGGGTGTCGCCGGCACGATGGCGGC
AAATGGCTCGGGCCTACAGATGGCCACGATCCGCAACCTGCTGATGGCCTGGGTGCTGACGCTGCCGGCCGCGATCCTGC
TATCGGCGACCCTGTACGTACTGCTGTCGCGCGTGCTCTGA

Upstream 100 bases:

>100_bases
CACCCCCCGCCGTAGATCCGCAACTCCATCGACATCCAGCTGACCGACAAGTGTCATCGGAGCGTCAGATCGGGCGGGGA
ATATCGCGCCACTCAATAAA

Downstream 100 bases:

>100_bases
GCCTGCACCGCGGCCGCGGTGACGACCGACGCTGTCGTACGGCCGCGGCCCCGTAAAAAAGACGCCGCTCCGGCAGAACC
GGAACGGCGTCAGGAGGGAG

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MGRSVTDFTINQAIDDNGPLQPAGRPNLNKGFNPLTMILFFGVLGVGILFVAYSIYVDVEATGVRVTTYLPYLLLLVALL
IALGFEFVNGFHDTANAVATVIYTHSLPAEVAVVWSGVFNFLGVLTSTGAVAFGIVSLLPVELILQVGSSAGFAMVFALL
IAAILWNLGTWYFGLPASSSHTLIGSIIGVGVANALMRGRDGTSGVDWGKATEIGYALLLSPLVGFVCAAILLLLLKLVV
RNPKLYSAPEGNAPPPWWIRGLLILTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAYALNRALPDSQVAHFEQTSTAAS
AVIAAKGAGYSIIGDPRPAVTQYVSQHKISEGTYPSLAVLVKDIGEQVQRYGSLSKVPAESVGNTRNDMYLASEAIRFLM
KDKESDLSKDDVAKLNAYKGSLDSATKFIPNWVKIAVAIALGLGTMIGWKRIVITVGEKIGKSHLTYAQGASAELVAAST
IGAADMFGLPVSTTHVLSSGVAGTMAANGSGLQMATIRNLLMAWVLTLPAAILLSATLYVLLSRVL

Sequences:

>Translated_546_residues
MGRSVTDFTINQAIDDNGPLQPAGRPNLNKGFNPLTMILFFGVLGVGILFVAYSIYVDVEATGVRVTTYLPYLLLLVALL
IALGFEFVNGFHDTANAVATVIYTHSLPAEVAVVWSGVFNFLGVLTSTGAVAFGIVSLLPVELILQVGSSAGFAMVFALL
IAAILWNLGTWYFGLPASSSHTLIGSIIGVGVANALMRGRDGTSGVDWGKATEIGYALLLSPLVGFVCAAILLLLLKLVV
RNPKLYSAPEGNAPPPWWIRGLLILTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAYALNRALPDSQVAHFEQTSTAAS
AVIAAKGAGYSIIGDPRPAVTQYVSQHKISEGTYPSLAVLVKDIGEQVQRYGSLSKVPAESVGNTRNDMYLASEAIRFLM
KDKESDLSKDDVAKLNAYKGSLDSATKFIPNWVKIAVAIALGLGTMIGWKRIVITVGEKIGKSHLTYAQGASAELVAAST
IGAADMFGLPVSTTHVLSSGVAGTMAANGSGLQMATIRNLLMAWVLTLPAAILLSATLYVLLSRVL
>Mature_545_residues
GRSVTDFTINQAIDDNGPLQPAGRPNLNKGFNPLTMILFFGVLGVGILFVAYSIYVDVEATGVRVTTYLPYLLLLVALLI
ALGFEFVNGFHDTANAVATVIYTHSLPAEVAVVWSGVFNFLGVLTSTGAVAFGIVSLLPVELILQVGSSAGFAMVFALLI
AAILWNLGTWYFGLPASSSHTLIGSIIGVGVANALMRGRDGTSGVDWGKATEIGYALLLSPLVGFVCAAILLLLLKLVVR
NPKLYSAPEGNAPPPWWIRGLLILTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAYALNRALPDSQVAHFEQTSTAASA
VIAAKGAGYSIIGDPRPAVTQYVSQHKISEGTYPSLAVLVKDIGEQVQRYGSLSKVPAESVGNTRNDMYLASEAIRFLMK
DKESDLSKDDVAKLNAYKGSLDSATKFIPNWVKIAVAIALGLGTMIGWKRIVITVGEKIGKSHLTYAQGASAELVAASTI
GAADMFGLPVSTTHVLSSGVAGTMAANGSGLQMATIRNLLMAWVLTLPAAILLSATLYVLLSRVL

Specific function: Low-affinity inorganic phosphate transport. Can also transport arsenate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=478, Percent_Identity=45.397489539749, Blast_Score=380, Evalue=1e-106,
Organism=Escherichia coli, GI1789360, Length=480, Percent_Identity=42.2916666666667, Blast_Score=358, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25146401, Length=409, Percent_Identity=23.7163814180929, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 57417; Mature: 57286

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRSVTDFTINQAIDDNGPLQPAGRPNLNKGFNPLTMILFFGVLGVGILFVAYSIYVDVE
CCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEE
ATGVRVTTYLPYLLLLVALLIALGFEFVNGFHDTANAVATVIYTHSLPAEVAVVWSGVFN
ECCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
FLGVLTSTGAVAFGIVSLLPVELILQVGSSAGFAMVFALLIAAILWNLGTWYFGLPASSS
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCC
HTLIGSIIGVGVANALMRGRDGTSGVDWGKATEIGYALLLSPLVGFVCAAILLLLLKLVV
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNPKLYSAPEGNAPPPWWIRGLLILTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAYAL
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCCHHHHHH
NRALPDSQVAHFEQTSTAASAVIAAKGAGYSIIGDPRPAVTQYVSQHKISEGTYPSLAVL
HHCCCCHHHHHHHHHHHHHHHHHEECCCCEEECCCCCHHHHHHHHHHHCCCCCCHHHHHH
VKDIGEQVQRYGSLSKVPAESVGNTRNDMYLASEAIRFLMKDKESDLSKDDVAKLNAYKG
HHHHHHHHHHHCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
SLDSATKFIPNWVKIAVAIALGLGTMIGWKRIVITVGEKIGKSHLTYAQGASAELVAAST
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHCCHHCCHHCCCCCCEEEHHH
IGAADMFGLPVSTTHVLSSGVAGTMAANGSGLQMATIRNLLMAWVLTLPAAILLSATLYV
CCHHHHCCCCCCHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLSRVL
HHHHHC
>Mature Secondary Structure 
GRSVTDFTINQAIDDNGPLQPAGRPNLNKGFNPLTMILFFGVLGVGILFVAYSIYVDVE
CCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEE
ATGVRVTTYLPYLLLLVALLIALGFEFVNGFHDTANAVATVIYTHSLPAEVAVVWSGVFN
ECCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
FLGVLTSTGAVAFGIVSLLPVELILQVGSSAGFAMVFALLIAAILWNLGTWYFGLPASSS
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCC
HTLIGSIIGVGVANALMRGRDGTSGVDWGKATEIGYALLLSPLVGFVCAAILLLLLKLVV
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNPKLYSAPEGNAPPPWWIRGLLILTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAYAL
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCCHHHHHH
NRALPDSQVAHFEQTSTAASAVIAAKGAGYSIIGDPRPAVTQYVSQHKISEGTYPSLAVL
HHCCCCHHHHHHHHHHHHHHHHHEECCCCEEECCCCCHHHHHHHHHHHCCCCCCHHHHHH
VKDIGEQVQRYGSLSKVPAESVGNTRNDMYLASEAIRFLMKDKESDLSKDDVAKLNAYKG
HHHHHHHHHHHCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
SLDSATKFIPNWVKIAVAIALGLGTMIGWKRIVITVGEKIGKSHLTYAQGASAELVAAST
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHCCHHCCHHCCCCCCEEEHHH
IGAADMFGLPVSTTHVLSSGVAGTMAANGSGLQMATIRNLLMAWVLTLPAAILLSATLYV
CCHHHHCCCCCCHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLSRVL
HHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]