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The map label for this gene is nrdB [H]

Identifier: 192291019

GI number: 192291019

Start: 2859390

End: 2860511

Strand: Reverse

Name: nrdB [H]

Synonym: Rpal_2638

Alternate gene names: 192291019

Gene position: 2860511-2859390 (Counterclockwise)

Preceding gene: 192291020

Following gene: 192291018

Centisome position: 49.8

GC content: 61.41

Gene sequence:

>1122_bases
ATGCTGCAAGCGATCAAGAACCAGGACGCCCACCTCGTCGCGAATCTCGCCAACACCGCGCAAGCGCCGGATACGACCGG
GCTCGGCACGATCGATCGCGGCGCCGCGCGCGTGCGCGTCGACGACAAGCGGATGATCAACGCGCGCGCCGACGTCAACC
AGCTATTGCCGCTGAAATATCATTGGGCCTGGGAAAAATACCTCGCCGCCTGCAACAACCATTGGATGCCGACCGAGGTG
TCGATGCAGGCCGACATCGCGCTGTGGAAGTCGCGCGACGGCCTGACCGAGGATGAGCGCCACATGGTGAAGCGCAATCT
CGGCTTCTTCGCCGCGTCGGAGTCGCTGGTCGCCAACAATATCGTGCTGGCGATCTACCGCCATCTCACCAATCCCGAAT
GCCGGCAATATCTGCTGCGGCAGGCTTTCGAGGAGGCGGTGCACACCCACACCTTCCAGTACATCGTCGAAAGCCTCGGG
CTCGACGAGGGCGAACTGTTCAACATGTATCGCGAGGTCCCGTCGATCACCGACAAGGCGGCCTGGGCGCTCGACTACAC
CAAGCATCTCGACGATCCGCATTTCGAGACCGGCACCCCCGCGACCGATCAGGCGTTCCTGCTTGATCTGATCGCGTTCT
ACGTCGTTTTCGAAGGGATGTGGTTCTACACCGGCTTCGCACAGATCCTGTCGCTCGGCCGCCGCCAGAAGATGACCGGC
ATCGCCGAGCAGTATCAGTACATTCTGCGCGACGAGTCGATTCACTTGAACTTCGGCATCGACGTCATCAACCAGATCAA
GCTCGAAAACGCTGCGGTGTGGACGGCGTCGTTTCAGGACGACATCCGCGGCATGATCCACGACGCCGCCGAGCTCGAAG
CCGCGTATGGCCGGGATACGATGCCCCACGGCCTGCTGGGGCTGAACGCGGCATTGTGCGAGAGCTATATGCACTTCATC
GCCAACCGCCGCTGCGTTCAGCTCGGGCTTCGCCCGGTGTTCGCCGAGACCGACAATCCGTTCCCCTGGATGTCGGAGAC
GATGGACCTGAAGAAGGAGAAGAATTTCTTCGAGACCCGCGTCATCGAATACCAGAACGGCGGGGCGCTGGATTGGGACT
GA

Upstream 100 bases:

>100_bases
CCGGGAACGGCCACGCAGCCACCGAAGGCTGGGGCAAGGCTTGCGCCATCGACGATCCGGGTTGCGAGGCGTGCCAGTGA
GCGAACGAGGAGTTTTGACC

Downstream 100 bases:

>100_bases
ACGATGCAATCACCTCATTCGAATCCGGCAGCCCGCGGCGGGCGCCGGACCGAGCCGATCGAGCCGCAACCCGGTTCAGT
CGTAGCATCGAACGCGGCAC

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MLQAIKNQDAHLVANLANTAQAPDTTGLGTIDRGAARVRVDDKRMINARADVNQLLPLKYHWAWEKYLAACNNHWMPTEV
SMQADIALWKSRDGLTEDERHMVKRNLGFFAASESLVANNIVLAIYRHLTNPECRQYLLRQAFEEAVHTHTFQYIVESLG
LDEGELFNMYREVPSITDKAAWALDYTKHLDDPHFETGTPATDQAFLLDLIAFYVVFEGMWFYTGFAQILSLGRRQKMTG
IAEQYQYILRDESIHLNFGIDVINQIKLENAAVWTASFQDDIRGMIHDAAELEAAYGRDTMPHGLLGLNAALCESYMHFI
ANRRCVQLGLRPVFAETDNPFPWMSETMDLKKEKNFFETRVIEYQNGGALDWD

Sequences:

>Translated_373_residues
MLQAIKNQDAHLVANLANTAQAPDTTGLGTIDRGAARVRVDDKRMINARADVNQLLPLKYHWAWEKYLAACNNHWMPTEV
SMQADIALWKSRDGLTEDERHMVKRNLGFFAASESLVANNIVLAIYRHLTNPECRQYLLRQAFEEAVHTHTFQYIVESLG
LDEGELFNMYREVPSITDKAAWALDYTKHLDDPHFETGTPATDQAFLLDLIAFYVVFEGMWFYTGFAQILSLGRRQKMTG
IAEQYQYILRDESIHLNFGIDVINQIKLENAAVWTASFQDDIRGMIHDAAELEAAYGRDTMPHGLLGLNAALCESYMHFI
ANRRCVQLGLRPVFAETDNPFPWMSETMDLKKEKNFFETRVIEYQNGGALDWD
>Mature_373_residues
MLQAIKNQDAHLVANLANTAQAPDTTGLGTIDRGAARVRVDDKRMINARADVNQLLPLKYHWAWEKYLAACNNHWMPTEV
SMQADIALWKSRDGLTEDERHMVKRNLGFFAASESLVANNIVLAIYRHLTNPECRQYLLRQAFEEAVHTHTFQYIVESLG
LDEGELFNMYREVPSITDKAAWALDYTKHLDDPHFETGTPATDQAFLLDLIAFYVVFEGMWFYTGFAQILSLGRRQKMTG
IAEQYQYILRDESIHLNFGIDVINQIKLENAAVWTASFQDDIRGMIHDAAELEAAYGRDTMPHGLLGLNAALCESYMHFI
ANRRCVQLGLRPVFAETDNPFPWMSETMDLKKEKNFFETRVIEYQNGGALDWD

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=358, Percent_Identity=27.3743016759777, Blast_Score=148, Evalue=7e-36,
Organism=Homo sapiens, GI289177074, Length=347, Percent_Identity=27.6657060518732, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI260064013, Length=340, Percent_Identity=28.8235294117647, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI4557845, Length=340, Percent_Identity=28.8235294117647, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI289177076, Length=290, Percent_Identity=30.3448275862069, Blast_Score=131, Evalue=1e-30,
Organism=Escherichia coli, GI1788567, Length=293, Percent_Identity=22.8668941979522, Blast_Score=71, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17551968, Length=319, Percent_Identity=30.0940438871473, Blast_Score=140, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI193206379, Length=304, Percent_Identity=24.3421052631579, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17567153, Length=224, Percent_Identity=25.4464285714286, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322434, Length=330, Percent_Identity=30.9090909090909, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6321619, Length=329, Percent_Identity=22.4924012158055, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI18079279, Length=319, Percent_Identity=30.0940438871473, Blast_Score=145, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 42708; Mature: 42708

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00603 TK_CELLULAR_TYPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQAIKNQDAHLVANLANTAQAPDTTGLGTIDRGAARVRVDDKRMINARADVNQLLPLKY
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECHHHHHHHHCCHHHCCCCEE
HWAWEKYLAACNNHWMPTEVSMQADIALWKSRDGLTEDERHMVKRNLGFFAASESLVANN
HHHHHHHHHHHCCCCCCCCCCHHHHHHEECCCCCCCHHHHHHHHHHCCHHCCCCHHHHHH
IVLAIYRHLTNPECRQYLLRQAFEEAVHTHTFQYIVESLGLDEGELFNMYREVPSITDKA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHH
AWALDYTKHLDDPHFETGTPATDQAFLLDLIAFYVVFEGMWFYTGFAQILSLGRRQKMTG
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAEQYQYILRDESIHLNFGIDVINQIKLENAAVWTASFQDDIRGMIHDAAELEAAYGRDT
HHHHHHHHHCCCCEEEEECHHHHHHHEECCCEEEEECHHHHHHHHHHHHHHHHHHHCCCC
MPHGLLGLNAALCESYMHFIANRRCVQLGLRPVFAETDNPFPWMSETMDLKKEKNFFETR
CCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHH
VIEYQNGGALDWD
EEEECCCCCCCCC
>Mature Secondary Structure
MLQAIKNQDAHLVANLANTAQAPDTTGLGTIDRGAARVRVDDKRMINARADVNQLLPLKY
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECHHHHHHHHCCHHHCCCCEE
HWAWEKYLAACNNHWMPTEVSMQADIALWKSRDGLTEDERHMVKRNLGFFAASESLVANN
HHHHHHHHHHHCCCCCCCCCCHHHHHHEECCCCCCCHHHHHHHHHHCCHHCCCCHHHHHH
IVLAIYRHLTNPECRQYLLRQAFEEAVHTHTFQYIVESLGLDEGELFNMYREVPSITDKA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHH
AWALDYTKHLDDPHFETGTPATDQAFLLDLIAFYVVFEGMWFYTGFAQILSLGRRQKMTG
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAEQYQYILRDESIHLNFGIDVINQIKLENAAVWTASFQDDIRGMIHDAAELEAAYGRDT
HHHHHHHHHCCCCEEEEECHHHHHHHEECCCEEEEECHHHHHHHHHHHHHHHHHHHCCCC
MPHGLLGLNAALCESYMHFIANRRCVQLGLRPVFAETDNPFPWMSETMDLKKEKNFFETR
CCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHH
VIEYQNGGALDWD
EEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]