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The map label for this gene is ltaA [H]

Identifier: 192290992

GI number: 192290992

Start: 2821768

End: 2822868

Strand: Reverse

Name: ltaA [H]

Synonym: Rpal_2609

Alternate gene names: 192290992

Gene position: 2822868-2821768 (Counterclockwise)

Preceding gene: 192290994

Following gene: 192290985

Centisome position: 49.14

GC content: 67.39

Gene sequence:

>1101_bases
GTGATCTACGCCCCCGCCCCTGACGATCCGCGACTGCCGCCGGTGCGGATCAATTTGCTGTCGGACACTCAGACGCAGCC
CAGTGCGGCGATGCGCGAGGCGATGGCGCGGGCGCCTGTGGGAGACGAACAGATCGGCGCCGATCCGACCGTGAACGCGC
TGTGCGCCCGTGTCGCCGATCTGCTCGGCAAGGAGGCGGCGGTGTTCATGGCGTCCGGCACGATCTGCAACATTGCGGCG
ACGTTGGCGCATTGCCGAGCCGGCGACGAAATTCTCGCGCACGAGACGTCCCATGTCGTCTCGCTGGAGGGCTGCACACA
CGCGGCTCTCGGTGGCTTTCAGATCCACCCGCTGCCGGGCGCAGGCGGTCAGTTCTCCGCTGAAACGCTGCGATCGGCGC
TGCATCGGCCAGACCGGCATCAGGCGAAGCAGAGCTTGGTGTGCGTCGAGCAGACCACCAATCTCGGCGGCGGCACGGTG
TGGAACGCTTTGACGCTCCGGCAGATCGCGGAATTGGCTCGCGCCAATGGATTAGCCACCCATATGGACGGTGCTCGCCT
GATGAACGCCAGCGTCGCCAGCGGCATCTCGGCCCGGCAGATCGCCGCCGGCTGGGACTCGGTGTGGATCGACTTCAGCA
AGGGCCTCGGCGCGCCGCTCGGTGCCGTGCTGGCGGGATCGCGCGATTTCATCGACGAGGTCTGGCTGTGGAAGCAGCGG
CTCGGCGGGTCGCTGCGTCAGGCCGGCATTTGCGCCGCCGCCTGCCTGTATGCGCTCGACCACAATGTCGGGCGACTTGC
TGAGGATCACGCCAATGCCCGACTGCTCGCGGCAGGCTTGTCGCAGATCGACGGCGTGAGCGCTGAACCGCCGGAGACCA
ACCTGGTCTATTTCGACGTCTCCGGCGCCGCGTGGTCAAAGCAGGCATTGGTCGACGCGCTGCGCGCCCGTGGTGTGCTG
ATCTCGATCCTCAGCGGCCGGCTACGCGCCTGCGCGCATCTCGACGTCACGCCCGCGATGATCCAAGAGGCGCTCGCAGT
GATCCGCGACGTGGTCGAAGGCGATACGACGGCCCGGATCGGCCGCAGCGCACAGATCTGA

Upstream 100 bases:

>100_bases
TTGTAATGGTCCAACGAATAATGTTGGCTTCCGCCCGGTAAGTACCCGCGACGGCCGGCGTCGCATGGCCACCCAGTCTC
CATCAGCGTGACATCGCACC

Downstream 100 bases:

>100_bases
TCTGAGCTGAGCGCCGTCATGCCATGGTGCTGGCGCGCACCACCGTGGTGTAGGTTCGCTCGATCAATTGCAACGCGCCG
CGATAGCCGACATAGGCGCG

Product: Threonine aldolase

Products: Acetaldehyde; Glycine [C]

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MIYAPAPDDPRLPPVRINLLSDTQTQPSAAMREAMARAPVGDEQIGADPTVNALCARVADLLGKEAAVFMASGTICNIAA
TLAHCRAGDEILAHETSHVVSLEGCTHAALGGFQIHPLPGAGGQFSAETLRSALHRPDRHQAKQSLVCVEQTTNLGGGTV
WNALTLRQIAELARANGLATHMDGARLMNASVASGISARQIAAGWDSVWIDFSKGLGAPLGAVLAGSRDFIDEVWLWKQR
LGGSLRQAGICAAACLYALDHNVGRLAEDHANARLLAAGLSQIDGVSAEPPETNLVYFDVSGAAWSKQALVDALRARGVL
ISILSGRLRACAHLDVTPAMIQEALAVIRDVVEGDTTARIGRSAQI

Sequences:

>Translated_366_residues
MIYAPAPDDPRLPPVRINLLSDTQTQPSAAMREAMARAPVGDEQIGADPTVNALCARVADLLGKEAAVFMASGTICNIAA
TLAHCRAGDEILAHETSHVVSLEGCTHAALGGFQIHPLPGAGGQFSAETLRSALHRPDRHQAKQSLVCVEQTTNLGGGTV
WNALTLRQIAELARANGLATHMDGARLMNASVASGISARQIAAGWDSVWIDFSKGLGAPLGAVLAGSRDFIDEVWLWKQR
LGGSLRQAGICAAACLYALDHNVGRLAEDHANARLLAAGLSQIDGVSAEPPETNLVYFDVSGAAWSKQALVDALRARGVL
ISILSGRLRACAHLDVTPAMIQEALAVIRDVVEGDTTARIGRSAQI
>Mature_366_residues
MIYAPAPDDPRLPPVRINLLSDTQTQPSAAMREAMARAPVGDEQIGADPTVNALCARVADLLGKEAAVFMASGTICNIAA
TLAHCRAGDEILAHETSHVVSLEGCTHAALGGFQIHPLPGAGGQFSAETLRSALHRPDRHQAKQSLVCVEQTTNLGGGTV
WNALTLRQIAELARANGLATHMDGARLMNASVASGISARQIAAGWDSVWIDFSKGLGAPLGAVLAGSRDFIDEVWLWKQR
LGGSLRQAGICAAACLYALDHNVGRLAEDHANARLLAAGLSQIDGVSAEPPETNLVYFDVSGAAWSKQALVDALRARGVL
ISILSGRLRACAHLDVTPAMIQEALAVIRDVVEGDTTARIGRSAQI

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=331, Percent_Identity=40.785498489426, Blast_Score=218, Evalue=7e-58,
Organism=Caenorhabditis elegans, GI193206524, Length=346, Percent_Identity=35.5491329479769, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI193206520, Length=346, Percent_Identity=35.5491329479769, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI193206522, Length=346, Percent_Identity=35.5491329479769, Blast_Score=182, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6320789, Length=354, Percent_Identity=30.7909604519774, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI21355439, Length=298, Percent_Identity=40.2684563758389, Blast_Score=196, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5 [C]

Molecular weight: Translated: 38400; Mature: 38400

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYAPAPDDPRLPPVRINLLSDTQTQPSAAMREAMARAPVGDEQIGADPTVNALCARVAD
CCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
LLGKEAAVFMASGTICNIAATLAHCRAGDEILAHETSHVVSLEGCTHAALGGFQIHPLPG
HHCCCCEEEEECCCHHHHHHHHHHHCCCCHHHHCCCCCEEEECCCCHHHCCCEEEECCCC
AGGQFSAETLRSALHRPDRHQAKQSLVCVEQTTNLGGGTVWNALTLRQIAELARANGLAT
CCCCCCHHHHHHHHCCCHHHHHHHHEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHCCCEE
HMDGARLMNASVASGISARQIAAGWDSVWIDFSKGLGAPLGAVLAGSRDFIDEVWLWKQR
CCCCHHHHHHHHHCCCCHHHHHCCCCCEEEECCCCCCCCHHHHHCCCHHHHHHHHHHHHH
LGGSLRQAGICAAACLYALDHNVGRLAEDHANARLLAAGLSQIDGVSAEPPETNLVYFDV
HCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCEEEEEC
SGAAWSKQALVDALRARGVLISILSGRLRACAHLDVTPAMIQEALAVIRDVVEGDTTARI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHC
GRSAQI
CCCCCC
>Mature Secondary Structure
MIYAPAPDDPRLPPVRINLLSDTQTQPSAAMREAMARAPVGDEQIGADPTVNALCARVAD
CCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
LLGKEAAVFMASGTICNIAATLAHCRAGDEILAHETSHVVSLEGCTHAALGGFQIHPLPG
HHCCCCEEEEECCCHHHHHHHHHHHCCCCHHHHCCCCCEEEECCCCHHHCCCEEEECCCC
AGGQFSAETLRSALHRPDRHQAKQSLVCVEQTTNLGGGTVWNALTLRQIAELARANGLAT
CCCCCCHHHHHHHHCCCHHHHHHHHEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHCCCEE
HMDGARLMNASVASGISARQIAAGWDSVWIDFSKGLGAPLGAVLAGSRDFIDEVWLWKQR
CCCCHHHHHHHHHCCCCHHHHHCCCCCEEEECCCCCCCCHHHHHCCCHHHHHHHHHHHHH
LGGSLRQAGICAAACLYALDHNVGRLAEDHANARLLAAGLSQIDGVSAEPPETNLVYFDV
HCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCEEEEEC
SGAAWSKQALVDALRARGVLISILSGRLRACAHLDVTPAMIQEALAVIRDVVEGDTTARI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHC
GRSAQI
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 0.22 {L-allo-Thr}} 2.85 {L-Thr}} 0.12 {DL-threo-phenylserine}} 0.24 {DL-erythro-phenylserine}} [C]

Substrates: L-Threonine [C]

Specific reaction: L-Threonine <==> Acetaldehyde + Glycine [C]

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]