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The map label for this gene is mdeA [H]

Identifier: 192290976

GI number: 192290976

Start: 2803736

End: 2804929

Strand: Reverse

Name: mdeA [H]

Synonym: Rpal_2593

Alternate gene names: 192290976

Gene position: 2804929-2803736 (Counterclockwise)

Preceding gene: 192290978

Following gene: 192290975

Centisome position: 48.83

GC content: 68.01

Gene sequence:

>1194_bases
ATGACCCAGCCGCACGCCGCCAATGTCGAAACGCTCGCGGTCCATGGTGGCTCGTTCCGCTCCGATCCGGTAACCGGCGC
GGTGGTGCCTCCGATCTATCTGACCACCTCGTACCAGTTTCAGGACACCGAGCACGCGCGCCGCATCTTCGCGCTCGAAG
AGATCGGCTACACCTATACACGCACCGTCAATCCCACCCGCGAGTTCCTGGAGCGCCGCGTCTCTGCGCTCGAAGGCGGC
GTCGGTGCACTTGCGCTCGCCACCGGCGAGAGCGCATCGCTGTACGCGCTGCTCAACATCGCCCACAGCGGCGACAACGT
GGTGCTGTCGCCCGACATCGTGACGGGACGCAATGCCGGTTTGGTCGCATCGCTGCGCCAGCTCGGGATCGACGTCCGTT
TCGCCGCAGAGACGGGATCGGCGGAGTCGTTCGCCACGGCGACCGATGCCCGCACCCGCGCCTATTATGTCGAGAGCCTC
AGCGTGCCGCAGCTGCGCGCCTTCCCGCTCGCCGGTGCAGCCGAAGCGGCCCAACAGGCCGGCGTGCCGCTGATCGTCGA
CAACACTGCGCAGCCGCTGTCAGTGAGGCCGTTCGAGCACGGCGCTGCGTTGGTGATCTACTCGGCAGCCGAATATCTCG
GCGGACATGACGGGGCCCAAGGCGGCTTGATCATCGACGGGGGGCGGTTTTCCTGGGAGGCTCACGGCACCCGGCTGCCG
ACCCTGAGCGAACCGGATGCGTCCTATCACGGCGTGGTCTGGACCGAGCTGGTGAAGAAATGGAACGCCAGCCCGTTCGT
GGCGCGGACGCGGGCACATCTGCTTCGCGATCTCGGCGCGGCGATCAATCCGCTGGCGGTGTTCCAGATCATCCAGGGTG
TCGAGACGCTGCCGCTGCGGCTGCGCCGGCAGACCGCCAACGCGCGCGAGGTCGCGACCTATCTGGGCGCGCACCCAAAG
GTGTCCGGCCTCGCCACGGGCGCCGCGCTGATCGCCTTCGACCTGCCGAGCGCAGCCGCTGCGGCGCGCTTCACCGACGC
ATTGACGCTGTTTGATCCTGCCGTCACTTACGGCGGTGTCCGCAGCGCCGTCCTCGACCGAACCTCGACCTCGCCGCGGG
TGGTGCTGTCGATCGGGCTCGAACACGCCGGCGACCTGCTCGCCGATCTCGACTCCGCCCTCGCCCACATCTGA

Upstream 100 bases:

>100_bases
TCTTTGCAACGGCAGCAGCGCCACTGAGCGATATGTCCGTCGCTCGACTTTCACCGATCGTTTCCCTCTCTTCACCTCTG
CCGCCGAGGAGCGCACCGTC

Downstream 100 bases:

>100_bases
TCGCAGCCGCGACGAGCAGATCTGGCAGCAATCCGGTAGCCGAGCATCATCGCACGGTGCCCCGCCGACACTGGATTGTT
TCTCCACACGCACGACAGCG

Product: Cys/Met metabolism pyridoxal-phosphate-dependent protein

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MTQPHAANVETLAVHGGSFRSDPVTGAVVPPIYLTTSYQFQDTEHARRIFALEEIGYTYTRTVNPTREFLERRVSALEGG
VGALALATGESASLYALLNIAHSGDNVVLSPDIVTGRNAGLVASLRQLGIDVRFAAETGSAESFATATDARTRAYYVESL
SVPQLRAFPLAGAAEAAQQAGVPLIVDNTAQPLSVRPFEHGAALVIYSAAEYLGGHDGAQGGLIIDGGRFSWEAHGTRLP
TLSEPDASYHGVVWTELVKKWNASPFVARTRAHLLRDLGAAINPLAVFQIIQGVETLPLRLRRQTANAREVATYLGAHPK
VSGLATGAALIAFDLPSAAAAARFTDALTLFDPAVTYGGVRSAVLDRTSTSPRVVLSIGLEHAGDLLADLDSALAHI

Sequences:

>Translated_397_residues
MTQPHAANVETLAVHGGSFRSDPVTGAVVPPIYLTTSYQFQDTEHARRIFALEEIGYTYTRTVNPTREFLERRVSALEGG
VGALALATGESASLYALLNIAHSGDNVVLSPDIVTGRNAGLVASLRQLGIDVRFAAETGSAESFATATDARTRAYYVESL
SVPQLRAFPLAGAAEAAQQAGVPLIVDNTAQPLSVRPFEHGAALVIYSAAEYLGGHDGAQGGLIIDGGRFSWEAHGTRLP
TLSEPDASYHGVVWTELVKKWNASPFVARTRAHLLRDLGAAINPLAVFQIIQGVETLPLRLRRQTANAREVATYLGAHPK
VSGLATGAALIAFDLPSAAAAARFTDALTLFDPAVTYGGVRSAVLDRTSTSPRVVLSIGLEHAGDLLADLDSALAHI
>Mature_396_residues
TQPHAANVETLAVHGGSFRSDPVTGAVVPPIYLTTSYQFQDTEHARRIFALEEIGYTYTRTVNPTREFLERRVSALEGGV
GALALATGESASLYALLNIAHSGDNVVLSPDIVTGRNAGLVASLRQLGIDVRFAAETGSAESFATATDARTRAYYVESLS
VPQLRAFPLAGAAEAAQQAGVPLIVDNTAQPLSVRPFEHGAALVIYSAAEYLGGHDGAQGGLIIDGGRFSWEAHGTRLPT
LSEPDASYHGVVWTELVKKWNASPFVARTRAHLLRDLGAAINPLAVFQIIQGVETLPLRLRRQTANAREVATYLGAHPKV
SGLATGAALIAFDLPSAAAAARFTDALTLFDPAVTYGGVRSAVLDRTSTSPRVVLSIGLEHAGDLLADLDSALAHI

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=381, Percent_Identity=25.1968503937008, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI25453487, Length=319, Percent_Identity=24.1379310344828, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1790375, Length=430, Percent_Identity=26.7441860465116, Blast_Score=108, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71980708, Length=418, Percent_Identity=24.1626794258373, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17560056, Length=380, Percent_Identity=25.2631578947368, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17538055, Length=376, Percent_Identity=25.531914893617, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6323333, Length=412, Percent_Identity=32.7669902912621, Blast_Score=190, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6319307, Length=438, Percent_Identity=25.1141552511416, Blast_Score=93, Evalue=9e-20,
Organism=Drosophila melanogaster, GI19922554, Length=423, Percent_Identity=25.7683215130024, Blast_Score=108, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 41925; Mature: 41793

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPHAANVETLAVHGGSFRSDPVTGAVVPPIYLTTSYQFQDTEHARRIFALEEIGYTYT
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHCCCEEE
RTVNPTREFLERRVSALEGGVGALALATGESASLYALLNIAHSGDNVVLSPDIVTGRNAG
ECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEEEEECCCCEEECCCEECCCCCH
LVASLRQLGIDVRFAAETGSAESFATATDARTRAYYVESLSVPQLRAFPLAGAAEAAQQA
HHHHHHHHCCEEEEEECCCCCCHHHHHCCCCCEEEEEECCCCCCEEECCCCCHHHHHHHC
GVPLIVDNTAQPLSVRPFEHGAALVIYSAAEYLGGHDGAQGGLIIDGGRFSWEAHGTRLP
CCEEEECCCCCCCEECCCCCCCEEEEEECHHHHCCCCCCCCCEEEECCEEEEEECCCCCC
TLSEPDASYHGVVWTELVKKWNASPFVARTRAHLLRDLGAAINPLAVFQIIQGVETLPLR
CCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHH
LRRQTANAREVATYLGAHPKVSGLATGAALIAFDLPSAAAAARFTDALTLFDPAVTYGGV
HHHHCCCHHHHHHHHCCCCCCCHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCH
RSAVLDRTSTSPRVVLSIGLEHAGDLLADLDSALAHI
HHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQPHAANVETLAVHGGSFRSDPVTGAVVPPIYLTTSYQFQDTEHARRIFALEEIGYTYT
CCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHCCCEEE
RTVNPTREFLERRVSALEGGVGALALATGESASLYALLNIAHSGDNVVLSPDIVTGRNAG
ECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEEEEECCCCEEECCCEECCCCCH
LVASLRQLGIDVRFAAETGSAESFATATDARTRAYYVESLSVPQLRAFPLAGAAEAAQQA
HHHHHHHHCCEEEEEECCCCCCHHHHHCCCCCEEEEEECCCCCCEEECCCCCHHHHHHHC
GVPLIVDNTAQPLSVRPFEHGAALVIYSAAEYLGGHDGAQGGLIIDGGRFSWEAHGTRLP
CCEEEECCCCCCCEECCCCCCCEEEEEECHHHHCCCCCCCCCEEEECCEEEEEECCCCCC
TLSEPDASYHGVVWTELVKKWNASPFVARTRAHLLRDLGAAINPLAVFQIIQGVETLPLR
CCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHH
LRRQTANAREVATYLGAHPKVSGLATGAALIAFDLPSAAAAARFTDALTLFDPAVTYGGV
HHHHCCCHHHHHHHHCCCCCCCHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCH
RSAVLDRTSTSPRVVLSIGLEHAGDLLADLDSALAHI
HHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]