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The map label for this gene is pyk1 [H]

Identifier: 192290896

GI number: 192290896

Start: 2711788

End: 2713266

Strand: Reverse

Name: pyk1 [H]

Synonym: Rpal_2513

Alternate gene names: 192290896

Gene position: 2713266-2711788 (Counterclockwise)

Preceding gene: 192290904

Following gene: 192290895

Centisome position: 47.24

GC content: 68.76

Gene sequence:

>1479_bases
ATGTCGACCGAAATCCTCCGCCTCAAGCACAAGCTCGACACGCTGATCACCGACATCGACGAGGGCAGCCGCGAGCGGCT
GGCCGGCTGGGCGGAGCAGATCCATCGCCCCGGCTTCGGCCCGAGCGCGGCCAACCTCGCCCACTATCTGGCGCTGCGTC
ACCACGATCTGCGCCCGCTGCAGCACGCCCTGATGGCGCTGGGACTGTCCTCGCTCGGCCGGCTCGAAAGCCGGGTGATG
CCGACGCTGCTCGCGGTGCGCGCCACCCTCGCGGCACTGGCGGGCGAGCCGGAAATGCTGCGGCCGTCGTCGAAGCAGTT
CTTCGCCGGCGAGCACGATCTCGCCAGCCGCAGCGCAGAGCTGTTCGGGCCGCTGTCGGGCGCCCGCCATTCCGCCCTGC
TGGTCACCTGCCCGAGCGAAGCTGCCGACGATCCGACCTTCATGCTGCGACTCGCCGAGCGCGGCGTCGAAGCGGTGCGG
ATCAACTGCGCCCACGACGATGCCGCCGCCTGGCAGCGCATGATCAACCACCTCCACGTCGCGGAGGAAGCCACCGGCCG
GCGGATGAAGGTGCTGATGGACATCGCCGGCCCCAAGATCCGCACCGGCGACGCGCGCACGCCCGACGGCCGCGACCGCA
TCGTCACCGGCGATCTGGTGGCGATCGTGGCGCAGGGCGAATTCGAGCGGATCGATCTCGATGACGAGCATTTCGCGGTG
GAATGCACCCTGCCCGATCCGCTGACCCGCGCCGCCGTCGGCGCCCGGGTGTTCGTCGATGACGGCAAGTTGTGCGTCCG
GATCGAGCGCAAGACCGCCTGGGGTCTGCTCGGCCGCGTCACCGCCACCGCCGACACCGGCTTCCGGCTGAAGCCGGAGA
AAGGCCTGAACTTCCCGGATACGCAGCTCGATATCCCGGCGCTGACCGCCAAGGACCGCGCAGATCTCGATTTCGTTGCG
GCCCATGCCGACGGCGTCGAGTTCTCGTTCGTGCAGTCGGTCGGCGACGTGAAGATGCTGCAGGCTGCGCTCGCCGAACG
CCGCCCCGACGACTGGCACAAACTGTCGCTGGTGTTGAAGATCGAGACGCCGGATGCGGTCGCGCACCTGCCGGACATCC
TGGTGCAGGCCGCCGGCCGCCAGCCGACCGCGGTGATGATCGCGCGCGGCGATCTCGCGGTGGAGATCGGCTTCGCCCGG
CTTGCCGAGATGCAGGAGGAGATCCTGTGGATCGGCGAAGCCGCCCATGTCCCGGTGATCTGGGCCACCCAGGTGCTCGA
ACATCTGGTGAAGAAGGGCACCCCGTCGCGCGGCGAAATGACCGACGCCGCGATGGCAGCGCGCGCCGAATGCGTGATGC
TGAACAAGGGCCCCTATCTGTTCAAGGCGATCACTGAGCTCGATACCCTGCTCGGCCGAATGGCCGACCATCAGCACAAG
AAGACGCCGCAGCTCCGGCGACTGCGCAGCTGGGGCTGA

Upstream 100 bases:

>100_bases
TGAGGCCGCGCAGCACCAGATGTCACATCGGCTTCATCCAGCACTCATAGAGCGGGTGGGATGCGGCTCTCCGGCCGCCA
GAACCTGCGAGTGCCCTGCG

Downstream 100 bases:

>100_bases
GCGCACTCACCTCTGGTATCAACCTAAGGTTGACTCGGCAATATCTTCGTTCACTCTATGTTCCATCAACGGAGCGCAGC
CGCTGCAGCGGCCAGAGTGA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 1 [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MSTEILRLKHKLDTLITDIDEGSRERLAGWAEQIHRPGFGPSAANLAHYLALRHHDLRPLQHALMALGLSSLGRLESRVM
PTLLAVRATLAALAGEPEMLRPSSKQFFAGEHDLASRSAELFGPLSGARHSALLVTCPSEAADDPTFMLRLAERGVEAVR
INCAHDDAAAWQRMINHLHVAEEATGRRMKVLMDIAGPKIRTGDARTPDGRDRIVTGDLVAIVAQGEFERIDLDDEHFAV
ECTLPDPLTRAAVGARVFVDDGKLCVRIERKTAWGLLGRVTATADTGFRLKPEKGLNFPDTQLDIPALTAKDRADLDFVA
AHADGVEFSFVQSVGDVKMLQAALAERRPDDWHKLSLVLKIETPDAVAHLPDILVQAAGRQPTAVMIARGDLAVEIGFAR
LAEMQEEILWIGEAAHVPVIWATQVLEHLVKKGTPSRGEMTDAAMAARAECVMLNKGPYLFKAITELDTLLGRMADHQHK
KTPQLRRLRSWG

Sequences:

>Translated_492_residues
MSTEILRLKHKLDTLITDIDEGSRERLAGWAEQIHRPGFGPSAANLAHYLALRHHDLRPLQHALMALGLSSLGRLESRVM
PTLLAVRATLAALAGEPEMLRPSSKQFFAGEHDLASRSAELFGPLSGARHSALLVTCPSEAADDPTFMLRLAERGVEAVR
INCAHDDAAAWQRMINHLHVAEEATGRRMKVLMDIAGPKIRTGDARTPDGRDRIVTGDLVAIVAQGEFERIDLDDEHFAV
ECTLPDPLTRAAVGARVFVDDGKLCVRIERKTAWGLLGRVTATADTGFRLKPEKGLNFPDTQLDIPALTAKDRADLDFVA
AHADGVEFSFVQSVGDVKMLQAALAERRPDDWHKLSLVLKIETPDAVAHLPDILVQAAGRQPTAVMIARGDLAVEIGFAR
LAEMQEEILWIGEAAHVPVIWATQVLEHLVKKGTPSRGEMTDAAMAARAECVMLNKGPYLFKAITELDTLLGRMADHQHK
KTPQLRRLRSWG
>Mature_491_residues
STEILRLKHKLDTLITDIDEGSRERLAGWAEQIHRPGFGPSAANLAHYLALRHHDLRPLQHALMALGLSSLGRLESRVMP
TLLAVRATLAALAGEPEMLRPSSKQFFAGEHDLASRSAELFGPLSGARHSALLVTCPSEAADDPTFMLRLAERGVEAVRI
NCAHDDAAAWQRMINHLHVAEEATGRRMKVLMDIAGPKIRTGDARTPDGRDRIVTGDLVAIVAQGEFERIDLDDEHFAVE
CTLPDPLTRAAVGARVFVDDGKLCVRIERKTAWGLLGRVTATADTGFRLKPEKGLNFPDTQLDIPALTAKDRADLDFVAA
HADGVEFSFVQSVGDVKMLQAALAERRPDDWHKLSLVLKIETPDAVAHLPDILVQAAGRQPTAVMIARGDLAVEIGFARL
AEMQEEILWIGEAAHVPVIWATQVLEHLVKKGTPSRGEMTDAAMAARAECVMLNKGPYLFKAITELDTLLGRMADHQHKK
TPQLRRLRSWG

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI10835121, Length=351, Percent_Identity=27.9202279202279, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI32967597, Length=351, Percent_Identity=27.9202279202279, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI33286418, Length=390, Percent_Identity=28.2051282051282, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI33286422, Length=375, Percent_Identity=28.2666666666667, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI33286420, Length=375, Percent_Identity=28.2666666666667, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI310128732, Length=312, Percent_Identity=26.2820512820513, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI310128730, Length=312, Percent_Identity=26.2820512820513, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI310128736, Length=256, Percent_Identity=25.390625, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI310128734, Length=256, Percent_Identity=25.390625, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI310128738, Length=233, Percent_Identity=26.1802575107296, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1787965, Length=342, Percent_Identity=28.6549707602339, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788160, Length=322, Percent_Identity=26.3975155279503, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17544584, Length=333, Percent_Identity=28.8288288288288, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17506831, Length=335, Percent_Identity=28.6567164179104, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71984413, Length=335, Percent_Identity=28.6567164179104, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17506829, Length=335, Percent_Identity=28.6567164179104, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71984406, Length=335, Percent_Identity=28.6567164179104, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6319279, Length=345, Percent_Identity=28.9855072463768, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324923, Length=324, Percent_Identity=30.2469135802469, Blast_Score=117, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24648964, Length=356, Percent_Identity=27.8089887640449, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI28571814, Length=356, Percent_Identity=27.8089887640449, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24648966, Length=298, Percent_Identity=29.5302013422819, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24581235, Length=355, Percent_Identity=27.0422535211268, Blast_Score=102, Evalue=8e-22,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 53993; Mature: 53862

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEILRLKHKLDTLITDIDEGSRERLAGWAEQIHRPGFGPSAANLAHYLALRHHDLRPL
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHH
QHALMALGLSSLGRLESRVMPTLLAVRATLAALAGEPEMLRPSSKQFFAGEHDLASRSAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHCCHH
LFGPLSGARHSALLVTCPSEAADDPTFMLRLAERGVEAVRINCAHDDAAAWQRMINHLHV
HHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHH
AEEATGRRMKVLMDIAGPKIRTGDARTPDGRDRIVTGDLVAIVAQGEFERIDLDDEHFAV
HHHHCCCEEEEEEECCCCCEECCCCCCCCCCCEEEECCEEEEEECCCEEEECCCCCCEEE
ECTLPDPLTRAAVGARVFVDDGKLCVRIERKTAWGLLGRVTATADTGFRLKPEKGLNFPD
EEECCCCHHHHHHCCEEEEECCCEEEEEECCHHHHHHHEEEEECCCCEEECCCCCCCCCC
TQLDIPALTAKDRADLDFVAAHADGVEFSFVQSVGDVKMLQAALAERRPDDWHKLSLVLK
CCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCHHHHHHHHHHHCCCCCCEEEEEEEE
IETPDAVAHLPDILVQAAGRQPTAVMIARGDLAVEIGFARLAEMQEEILWIGEAAHVPVI
EECCHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHEEECCCCCCCCH
WATQVLEHLVKKGTPSRGEMTDAAMAARAECVMLNKGPYLFKAITELDTLLGRMADHQHK
HHHHHHHHHHHCCCCCCCCHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHC
KTPQLRRLRSWG
CCHHHHHHHCCC
>Mature Secondary Structure 
STEILRLKHKLDTLITDIDEGSRERLAGWAEQIHRPGFGPSAANLAHYLALRHHDLRPL
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHH
QHALMALGLSSLGRLESRVMPTLLAVRATLAALAGEPEMLRPSSKQFFAGEHDLASRSAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHCCHH
LFGPLSGARHSALLVTCPSEAADDPTFMLRLAERGVEAVRINCAHDDAAAWQRMINHLHV
HHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHH
AEEATGRRMKVLMDIAGPKIRTGDARTPDGRDRIVTGDLVAIVAQGEFERIDLDDEHFAV
HHHHCCCEEEEEEECCCCCEECCCCCCCCCCCEEEECCEEEEEECCCEEEECCCCCCEEE
ECTLPDPLTRAAVGARVFVDDGKLCVRIERKTAWGLLGRVTATADTGFRLKPEKGLNFPD
EEECCCCHHHHHHCCEEEEECCCEEEEEECCHHHHHHHEEEEECCCCEEECCCCCCCCCC
TQLDIPALTAKDRADLDFVAAHADGVEFSFVQSVGDVKMLQAALAERRPDDWHKLSLVLK
CCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCHHHHHHHHHHHCCCCCCEEEEEEEE
IETPDAVAHLPDILVQAAGRQPTAVMIARGDLAVEIGFARLAEMQEEILWIGEAAHVPVI
EECCHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHEEECCCCCCCCH
WATQVLEHLVKKGTPSRGEMTDAAMAARAECVMLNKGPYLFKAITELDTLLGRMADHQHK
HHHHHHHHHHHCCCCCCCCHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHC
KTPQLRRLRSWG
CCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]