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The map label for this gene is hsrA [H]

Identifier: 192290877

GI number: 192290877

Start: 2689185

End: 2690660

Strand: Reverse

Name: hsrA [H]

Synonym: Rpal_2493

Alternate gene names: 192290877

Gene position: 2690660-2689185 (Counterclockwise)

Preceding gene: 192290884

Following gene: 192290874

Centisome position: 46.84

GC content: 65.51

Gene sequence:

>1476_bases
ATGAACAAAGATCGAGTTATTCCGCTGATCGTGGCTGCCGCGCTGTTCATGGAGAACATGGACGCGACCGTGATCGCGAC
CTCATTGCCGGCGATCGCCGCCGACATCGGCACCTCGCCGCTGACGCTGAAGCTGGCGATCACCTCGTATCTGTTGTCGC
TGGCGGTGTTCATTCCGGCGTCGGGCTGGACTGCTGATCGATTCGGCGCCCGCACCGTGTTCTCGATCGCGATCGCGGTG
TTCATGGTCGGCTCGATCGGCTGCGCGCTGTCGAGTTCGATCACCGATTTCGTCATCGCCCGCATCGTGCAGGGCATCGG
TGGGGCGATGATGACTCCTGTTGGGCGGTTGGTCCTGCTGCGCTCGATCGACAAGAGCGCGCTGGTCGGCGCGATGGCCT
GGGTCACGGTGCCGGCGCTGATCGGGCCGGTAATCGGCCCGCCGCTCGGCGGCTTCATCACCACCTACTTCTCCTGGCAC
TGGATCTTCCTGATCAACATTCCGATCGGCCTGATCGGTATCGCGATGGCGCAGCGCTTCATCGATCCGATCCGCAGCGA
TCATCTCGAAAAATTCGACCTGTGGGGGCTGGCGCTGGCCGGGATCGGCGTCGCCGGCATCGCTTTCGGCCTGTCGGTCG
CAGGCCTCGGCCTGCTGCACTGGACCGTGGTGGTCGGGCTGATCGGCGTCGGCGCGATCGCGATGACGCTCTACGTGTTC
CACGCCCGCCGCGTGCCGGCGCCGGTGCTGGACTTCTCGCTGCTGCGCCTGACGACGCTGCGCTCCAGCCTGATCGGCGG
TTTCCTGTTCCGGCTCGGCATCGGCGCGCTGCCGTTCCTGCTACCGCTCCTGATGCAGATCGGCTTCGGGCTGTCTCCGT
TTCAATCCGGCCTGGTTACGTTCGCCTCTGCGGCCGGCGCGATCGGCATGAAGACGCTGGCGGCGCGCATCATCCGCGCC
TTCGGCTTCCGCAACATCATGACGGTGAACGCGGTGGTCAGCTCGGTGTTTCTCGCCGCCTGCGCGCTGTTCACACCGGG
CACGCCGCTGCTGCTGATCATGATCATCCTGGTGGTCGGCGGCTTCTTCCGATCGCTGCAGTTCACCTCGATCAACACCG
TCGCGTATGCCGAGGTCGAGCAGGCGCAGATGAGCCGCGCCACCACCCTGCTCAGTGTCGGCCAGCAGCTCGCGCTATCG
GCAGGTGTTGCAGTCGGCGCGTTCTCGGTGGAAGCAACGATGGCTCTGCGCGGCGTCACCACGCTGGGCGCTGCGGACTT
TGCGCCCGCCTTCATCGTGGTGGCGGTGCCCGCCGCGCTGTCGGCGCTGTTCTTCTGGCAGATGCCGAGCGATGCCGGCC
ACGAGATTTCGGGACGCAAGGTGATCGAGATTTCGAGCAAGAAGGCAGGCGACAACGCTGCGACCAAGGCCGCGTCCGAA
CAGACCCAGGATGCCCGCGACCAGCGGTTCGGGTAA

Upstream 100 bases:

>100_bases
GCCGGTCACGGAGGATTTAGGTAACCGCGCAAGCTGCGCAATAGCCGGGGCCGCGACACGGTGGTACAGCGCGAGCGCCC
TCCTCTGGTCGACATCACTC

Downstream 100 bases:

>100_bases
GCCCGGTCCCCGGCGCTCAGCCGAAACGGTCGCCCCCGGACTGGGCGCCATCGTCTTGACGTACGGCCAGCCGCAGCACG
TCGTTGCAGATCGGGCAGCG

Product: major facilitator superfamily protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MNKDRVIPLIVAAALFMENMDATVIATSLPAIAADIGTSPLTLKLAITSYLLSLAVFIPASGWTADRFGARTVFSIAIAV
FMVGSIGCALSSSITDFVIARIVQGIGGAMMTPVGRLVLLRSIDKSALVGAMAWVTVPALIGPVIGPPLGGFITTYFSWH
WIFLINIPIGLIGIAMAQRFIDPIRSDHLEKFDLWGLALAGIGVAGIAFGLSVAGLGLLHWTVVVGLIGVGAIAMTLYVF
HARRVPAPVLDFSLLRLTTLRSSLIGGFLFRLGIGALPFLLPLLMQIGFGLSPFQSGLVTFASAAGAIGMKTLAARIIRA
FGFRNIMTVNAVVSSVFLAACALFTPGTPLLLIMIILVVGGFFRSLQFTSINTVAYAEVEQAQMSRATTLLSVGQQLALS
AGVAVGAFSVEATMALRGVTTLGAADFAPAFIVVAVPAALSALFFWQMPSDAGHEISGRKVIEISSKKAGDNAATKAASE
QTQDARDQRFG

Sequences:

>Translated_491_residues
MNKDRVIPLIVAAALFMENMDATVIATSLPAIAADIGTSPLTLKLAITSYLLSLAVFIPASGWTADRFGARTVFSIAIAV
FMVGSIGCALSSSITDFVIARIVQGIGGAMMTPVGRLVLLRSIDKSALVGAMAWVTVPALIGPVIGPPLGGFITTYFSWH
WIFLINIPIGLIGIAMAQRFIDPIRSDHLEKFDLWGLALAGIGVAGIAFGLSVAGLGLLHWTVVVGLIGVGAIAMTLYVF
HARRVPAPVLDFSLLRLTTLRSSLIGGFLFRLGIGALPFLLPLLMQIGFGLSPFQSGLVTFASAAGAIGMKTLAARIIRA
FGFRNIMTVNAVVSSVFLAACALFTPGTPLLLIMIILVVGGFFRSLQFTSINTVAYAEVEQAQMSRATTLLSVGQQLALS
AGVAVGAFSVEATMALRGVTTLGAADFAPAFIVVAVPAALSALFFWQMPSDAGHEISGRKVIEISSKKAGDNAATKAASE
QTQDARDQRFG
>Mature_491_residues
MNKDRVIPLIVAAALFMENMDATVIATSLPAIAADIGTSPLTLKLAITSYLLSLAVFIPASGWTADRFGARTVFSIAIAV
FMVGSIGCALSSSITDFVIARIVQGIGGAMMTPVGRLVLLRSIDKSALVGAMAWVTVPALIGPVIGPPLGGFITTYFSWH
WIFLINIPIGLIGIAMAQRFIDPIRSDHLEKFDLWGLALAGIGVAGIAFGLSVAGLGLLHWTVVVGLIGVGAIAMTLYVF
HARRVPAPVLDFSLLRLTTLRSSLIGGFLFRLGIGALPFLLPLLMQIGFGLSPFQSGLVTFASAAGAIGMKTLAARIIRA
FGFRNIMTVNAVVSSVFLAACALFTPGTPLLLIMIILVVGGFFRSLQFTSINTVAYAEVEQAQMSRATTLLSVGQQLALS
AGVAVGAFSVEATMALRGVTTLGAADFAPAFIVVAVPAALSALFFWQMPSDAGHEISGRKVIEISSKKAGDNAATKAASE
QTQDARDQRFG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=458, Percent_Identity=37.3362445414847, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1788392, Length=463, Percent_Identity=37.1490280777538, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1789042, Length=401, Percent_Identity=25.9351620947631, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1788710, Length=406, Percent_Identity=26.6009852216749, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI87081983, Length=313, Percent_Identity=27.7955271565495, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1790146, Length=135, Percent_Identity=31.1111111111111, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6325455, Length=297, Percent_Identity=25.9259259259259, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=162, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51403; Mature: 51403

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKDRVIPLIVAAALFMENMDATVIATSLPAIAADIGTSPLTLKLAITSYLLSLAVFIPA
CCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCC
SGWTADRFGARTVFSIAIAVFMVGSIGCALSSSITDFVIARIVQGIGGAMMTPVGRLVLL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RSIDKSALVGAMAWVTVPALIGPVIGPPLGGFITTYFSWHWIFLINIPIGLIGIAMAQRF
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEECCHHHHHHHHHHHH
IDPIRSDHLEKFDLWGLALAGIGVAGIAFGLSVAGLGLLHWTVVVGLIGVGAIAMTLYVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HARRVPAPVLDFSLLRLTTLRSSLIGGFLFRLGIGALPFLLPLLMQIGFGLSPFQSGLVT
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FASAAGAIGMKTLAARIIRAFGFRNIMTVNAVVSSVFLAACALFTPGTPLLLIMIILVVG
HHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GFFRSLQFTSINTVAYAEVEQAQMSRATTLLSVGQQLALSAGVAVGAFSVEATMALRGVT
HHHHHCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
TLGAADFAPAFIVVAVPAALSALFFWQMPSDAGHEISGRKVIEISSKKAGDNAATKAASE
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHH
QTQDARDQRFG
HHHHHHHCCCC
>Mature Secondary Structure
MNKDRVIPLIVAAALFMENMDATVIATSLPAIAADIGTSPLTLKLAITSYLLSLAVFIPA
CCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCC
SGWTADRFGARTVFSIAIAVFMVGSIGCALSSSITDFVIARIVQGIGGAMMTPVGRLVLL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RSIDKSALVGAMAWVTVPALIGPVIGPPLGGFITTYFSWHWIFLINIPIGLIGIAMAQRF
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEECCHHHHHHHHHHHH
IDPIRSDHLEKFDLWGLALAGIGVAGIAFGLSVAGLGLLHWTVVVGLIGVGAIAMTLYVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HARRVPAPVLDFSLLRLTTLRSSLIGGFLFRLGIGALPFLLPLLMQIGFGLSPFQSGLVT
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FASAAGAIGMKTLAARIIRAFGFRNIMTVNAVVSSVFLAACALFTPGTPLLLIMIILVVG
HHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GFFRSLQFTSINTVAYAEVEQAQMSRATTLLSVGQQLALSAGVAVGAFSVEATMALRGVT
HHHHHCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
TLGAADFAPAFIVVAVPAALSALFFWQMPSDAGHEISGRKVIEISSKKAGDNAATKAASE
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHH
QTQDARDQRFG
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]