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The map label for this gene is adhI [H]

Identifier: 192290557

GI number: 192290557

Start: 2344103

End: 2345212

Strand: Reverse

Name: adhI [H]

Synonym: Rpal_2166

Alternate gene names: 192290557

Gene position: 2345212-2344103 (Counterclockwise)

Preceding gene: 192290558

Following gene: 192290555

Centisome position: 40.83

GC content: 63.78

Gene sequence:

>1110_bases
ATGAAGACACGGGCCGCCGTCGCGTTCGAGGCCAAGAAGCCGCTCGAAATCGTCGAGTTGGATCTCGAAGGGCCGAAGGC
CGGCGAGGTGCTGGTCGAGATCAAGGCCACCGGCATCTGCCACACCGACGCCTACACGCTCGACGGCTTCGACTCTGAAG
GCATTTTCCCGTCGATCCTCGGCCATGAAGGCGCGGGCATCGTCCGCGAGGTCGGCCCCGGCGTCACCTCGGTGAAGCCG
GGCGATCACGTCATTCCGCTGTACACGCCGGAATGCCGGCAGTGCAAAAGCTGCCTGAGCCAGAAGACCAATCTCTGCAC
CGCGATCCGCGCCACGCAGGGCAAGGGCCTGATGCCGGACGGCACCTCGCGCTTTTCCTACAAGGGCCAGACCATCTATC
ACTACATGGGCTGCTCGACGTTCTCGAACTTCACCGTGCTGCCGGAGATAGCGGTCGCGAAGATCCGCGACGACGCGCCG
TTCGACAAGAGCTGCTACATCGGCTGCGGTGTCACCACCGGCGTCGGCGCCGTGGTCAACACCGCCAAGGTCACGCCCGG
CTCCAACGTCGTGGTGTTCGGCCTCGGCGGCATCGGTCTCAACGTGATCCAGGGCGCCAAGATGGTCGGTGCCGATAAAA
TCGTCGGCGTCGACATCAACGACGACAAGGAAGAATGGGGCCGCCGCTTCGGCATGACGCACTTCGTCAACCCGAAGAAG
GTCGACGGCGACATCGTCCAGCACCTCGTCGGGCTCACCGATGGCGGCGCCGACTACACCTTCGACTGCACCGGCAACAC
CAATGTGATGCGCCAGGCGCTGGAAGCCTGCCATCGCGGCTGGGGCGTCTCGGTGGTGATCGGCGTCGCTGAAGCCGGCA
AGGAAATCTCGACCCGCCCGTTCCAGCTCGTTACCGGCCGCGTCTGGAAGGGCAGCGCCTTCGGCGGCGCCCGCGGCCGT
ACCGACGTGCCGAAGATCGTCGACTGGTACATGAACGGCAAGATCGAGATCGACCCGATGATCACCCACGTGCTGAAACT
CGAAGAGATCAACAAGGGTTTCGACCTGATGCACGAAGGCAAGTCGATCCGCTCGGTGGTGGTGTACTAA

Upstream 100 bases:

>100_bases
GAGCCCCGTCGCCTCACCAGCCTAGAAAACGGTTCAAGTCGGCGCGCCGATTTGCGATACGAGAGGCAATCGCAGTTTTG
CCGATGCCAGGAGGATCGCC

Downstream 100 bases:

>100_bases
GCCAATACCCAACAAACTCCGTCATGCCCGGCCTCGTGCCGGGCATCGACGTCTTACGGCAGATATGGCGCGACAAGACG
TGGATGGCCGGTACAAGCCT

Product: S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MKTRAAVAFEAKKPLEIVELDLEGPKAGEVLVEIKATGICHTDAYTLDGFDSEGIFPSILGHEGAGIVREVGPGVTSVKP
GDHVIPLYTPECRQCKSCLSQKTNLCTAIRATQGKGLMPDGTSRFSYKGQTIYHYMGCSTFSNFTVLPEIAVAKIRDDAP
FDKSCYIGCGVTTGVGAVVNTAKVTPGSNVVVFGLGGIGLNVIQGAKMVGADKIVGVDINDDKEEWGRRFGMTHFVNPKK
VDGDIVQHLVGLTDGGADYTFDCTGNTNVMRQALEACHRGWGVSVVIGVAEAGKEISTRPFQLVTGRVWKGSAFGGARGR
TDVPKIVDWYMNGKIEIDPMITHVLKLEEINKGFDLMHEGKSIRSVVVY

Sequences:

>Translated_369_residues
MKTRAAVAFEAKKPLEIVELDLEGPKAGEVLVEIKATGICHTDAYTLDGFDSEGIFPSILGHEGAGIVREVGPGVTSVKP
GDHVIPLYTPECRQCKSCLSQKTNLCTAIRATQGKGLMPDGTSRFSYKGQTIYHYMGCSTFSNFTVLPEIAVAKIRDDAP
FDKSCYIGCGVTTGVGAVVNTAKVTPGSNVVVFGLGGIGLNVIQGAKMVGADKIVGVDINDDKEEWGRRFGMTHFVNPKK
VDGDIVQHLVGLTDGGADYTFDCTGNTNVMRQALEACHRGWGVSVVIGVAEAGKEISTRPFQLVTGRVWKGSAFGGARGR
TDVPKIVDWYMNGKIEIDPMITHVLKLEEINKGFDLMHEGKSIRSVVVY
>Mature_369_residues
MKTRAAVAFEAKKPLEIVELDLEGPKAGEVLVEIKATGICHTDAYTLDGFDSEGIFPSILGHEGAGIVREVGPGVTSVKP
GDHVIPLYTPECRQCKSCLSQKTNLCTAIRATQGKGLMPDGTSRFSYKGQTIYHYMGCSTFSNFTVLPEIAVAKIRDDAP
FDKSCYIGCGVTTGVGAVVNTAKVTPGSNVVVFGLGGIGLNVIQGAKMVGADKIVGVDINDDKEEWGRRFGMTHFVNPKK
VDGDIVQHLVGLTDGGADYTFDCTGNTNVMRQALEACHRGWGVSVVIGVAEAGKEISTRPFQLVTGRVWKGSAFGGARGR
TDVPKIVDWYMNGKIEIDPMITHVLKLEEINKGFDLMHEGKSIRSVVVY

Specific function: Oxidizes long-chain aliphatic alcohols, long-chain hydroxylated fatty acids and S-hydroxymethylglutathione (hmGSH) in increasing order of preference. Shows little or no activity with short-chain aliphatic alcohols [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=367, Percent_Identity=62.125340599455, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI71565152, Length=373, Percent_Identity=46.3806970509383, Blast_Score=359, Evalue=3e-99,
Organism=Homo sapiens, GI34577061, Length=369, Percent_Identity=46.6124661246612, Blast_Score=356, Evalue=2e-98,
Organism=Homo sapiens, GI262073058, Length=370, Percent_Identity=46.7567567567568, Blast_Score=348, Evalue=6e-96,
Organism=Homo sapiens, GI71743840, Length=370, Percent_Identity=46.7567567567568, Blast_Score=348, Evalue=6e-96,
Organism=Homo sapiens, GI4501929, Length=369, Percent_Identity=46.3414634146341, Blast_Score=347, Evalue=7e-96,
Organism=Homo sapiens, GI4501933, Length=369, Percent_Identity=45.7994579945799, Blast_Score=345, Evalue=3e-95,
Organism=Homo sapiens, GI156523966, Length=368, Percent_Identity=44.2934782608696, Blast_Score=339, Evalue=2e-93,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=44.5983379501385, Blast_Score=333, Evalue=2e-91,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=62.8726287262873, Blast_Score=466, Evalue=1e-133,
Organism=Escherichia coli, GI1788073, Length=364, Percent_Identity=26.6483516483516, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790045, Length=361, Percent_Identity=27.9778393351801, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87082125, Length=382, Percent_Identity=25.6544502617801, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787863, Length=360, Percent_Identity=24.7222222222222, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI87081918, Length=345, Percent_Identity=29.8550724637681, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788075, Length=369, Percent_Identity=27.10027100271, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI226510992, Length=364, Percent_Identity=26.0989010989011, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1786825, Length=378, Percent_Identity=24.8677248677249, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1788407, Length=225, Percent_Identity=26.2222222222222, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786518, Length=367, Percent_Identity=25.6130790190736, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25146526, Length=373, Percent_Identity=55.4959785522788, Blast_Score=433, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI71997431, Length=375, Percent_Identity=55.2, Blast_Score=429, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17565904, Length=343, Percent_Identity=55.3935860058309, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17562584, Length=371, Percent_Identity=30.9973045822102, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI71988145, Length=372, Percent_Identity=29.0322580645161, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17562582, Length=373, Percent_Identity=27.8820375335121, Blast_Score=91, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6320033, Length=371, Percent_Identity=57.9514824797844, Blast_Score=454, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6323099, Length=387, Percent_Identity=28.9405684754522, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322619, Length=359, Percent_Identity=28.6908077994429, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319955, Length=359, Percent_Identity=28.6908077994429, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319621, Length=370, Percent_Identity=26.2162162162162, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324486, Length=372, Percent_Identity=26.6129032258064, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323729, Length=373, Percent_Identity=26.8096514745308, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323961, Length=362, Percent_Identity=24.8618784530387, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319258, Length=276, Percent_Identity=28.2608695652174, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319257, Length=375, Percent_Identity=24.2666666666667, Blast_Score=76, Evalue=8e-15,
Organism=Drosophila melanogaster, GI17737895, Length=368, Percent_Identity=60.3260869565217, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI17137530, Length=338, Percent_Identity=27.810650887574, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI17737897, Length=339, Percent_Identity=24.4837758112094, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.1; =1.1.1.284 [H]

Molecular weight: Translated: 39604; Mature: 39604

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRAAVAFEAKKPLEIVELDLEGPKAGEVLVEIKATGICHTDAYTLDGFDSEGIFPSIL
CCCCEEEEEECCCCCEEEEEECCCCCCCCEEEEEEECEEEECCCEEECCCCCCCCCHHHH
GHEGAGIVREVGPGVTSVKPGDHVIPLYTPECRQCKSCLSQKTNLCTAIRATQGKGLMPD
CCCCCCHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHEEEEEECCCCCCCCC
GTSRFSYKGQTIYHYMGCSTFSNFTVLPEIAVAKIRDDAPFDKSCYIGCGVTTGVGAVVN
CCCCEEECCCEEEEEECCCCCCCCEECCCHHEEEECCCCCCCCCEEEECCCHHCCHHHEE
TAKVTPGSNVVVFGLGGIGLNVIQGAKMVGADKIVGVDINDDKEEWGRRFGMTHFVNPKK
EEEECCCCCEEEEEECCCCHHHHCCCEEECCCEEEEECCCCCHHHHHHHCCCCCCCCCCC
VDGDIVQHLVGLTDGGADYTFDCTGNTNVMRQALEACHRGWGVSVVIGVAEAGKEISTRP
CCHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCC
FQLVTGRVWKGSAFGGARGRTDVPKIVDWYMNGKIEIDPMITHVLKLEEINKGFDLMHEG
EEEEECEEEECCCCCCCCCCCCCHHHHHHHHCCEEEECHHHHHHHHHHHHCCCHHHHHCC
KSIRSVVVY
CCCEEEEEC
>Mature Secondary Structure
MKTRAAVAFEAKKPLEIVELDLEGPKAGEVLVEIKATGICHTDAYTLDGFDSEGIFPSIL
CCCCEEEEEECCCCCEEEEEECCCCCCCCEEEEEEECEEEECCCEEECCCCCCCCCHHHH
GHEGAGIVREVGPGVTSVKPGDHVIPLYTPECRQCKSCLSQKTNLCTAIRATQGKGLMPD
CCCCCCHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHEEEEEECCCCCCCCC
GTSRFSYKGQTIYHYMGCSTFSNFTVLPEIAVAKIRDDAPFDKSCYIGCGVTTGVGAVVN
CCCCEEECCCEEEEEECCCCCCCCEECCCHHEEEECCCCCCCCCEEEECCCHHCCHHHEE
TAKVTPGSNVVVFGLGGIGLNVIQGAKMVGADKIVGVDINDDKEEWGRRFGMTHFVNPKK
EEEECCCCCEEEEEECCCCHHHHCCCEEECCCEEEEECCCCCHHHHHHHCCCCCCCCCCC
VDGDIVQHLVGLTDGGADYTFDCTGNTNVMRQALEACHRGWGVSVVIGVAEAGKEISTRP
CCHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCC
FQLVTGRVWKGSAFGGARGRTDVPKIVDWYMNGKIEIDPMITHVLKLEEINKGFDLMHEG
EEEEECEEEECCCCCCCCCCCCCHHHHHHHHCCEEEECHHHHHHHHHHHHCCCHHHHHCC
KSIRSVVVY
CCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8631716 [H]