Click here to switch to the map view.

The map label for this gene is braD [H]

Identifier: 192290333

GI number: 192290333

Start: 2092644

End: 2093519

Strand: Reverse

Name: braD [H]

Synonym: Rpal_1940

Alternate gene names: 192290333

Gene position: 2093519-2092644 (Counterclockwise)

Preceding gene: 192290334

Following gene: 192290332

Centisome position: 36.45

GC content: 64.61

Gene sequence:

>876_bases
ATGCTCGCCCTGCAAATCCTAATCGACGGCTTCGCCATCAGCGCGCTGTATGCGCTGGGCGCGACCGGGTTTACGTTGAT
CTTCGGCGTCTCCGGCGTGCTCAACCTGTCACACGGCGCCATCATGGTCGCCGCCGCGGTCGCGGCCTGGGCAGCATCGA
GCACGTTCGGCATCGGCAGCTACGCCGGCGCGCTGATCGGCATCGCCACCGCGCTGGTGATCGCATTGCTGACCTATTTC
GCGGTGGTGCAGCCGATCCAGCGGTCACGGCGGATCGCCAATGAGGAGAAGGAGATCTTCGTCCTCACCGGCACCCTTCT
CTGGGGCATCATGATCCAGGAGGCGATCGCTTACTTTTTCACCAACAACGCCAAGACGGTGTTGCCGATCGTCACCGGCG
TGGTCGAAATTCTCGGCGTCCGCACCCCGCGCAACGAGATCTTCACCGCGCTGGTGTGCTGCCTGGTGATCGCGCTGCTG
TGGCTGCTGGTCAACCGCACCAAGATCGGCAAGGCGGTGCTGGCGGCCTCGATGAACCCGCGCGGCGTCACCCTGCTTGG
GCTCGAACTCACCAACATCTACGTCGTCGTCTGGATCATCTACGGCGTGCTCGCCGGTATCGCCGGGGTGCTGCTCGGCA
TGTTCCTGGGCGTGTCGTCCTACAGCGTCGGGCCGCTGACCGCGAGCGCATTCTCGATCGTGGTGCTCGGCGGGCTCGGC
AGCGTCTCCGGCTCGCTGATCGCCGCCTTCGTGGTCGGCTATCTGGAGACCTTCACCGCTTACATGATCTCGCCGGCCTA
TCGATCGATCCCGGCGCTGCTACTGTTGGTCACGGTGATGTATCTGCGGCCTCAGGGCCTGCTCGGGAGGCGCTGA

Upstream 100 bases:

>100_bases
GCGAACCCGGAATCTCAGAACAGCTCGGGATTCCGGGTCTGCGCGCCGGCGGCGCGCATCCCGGAATGACGTCCCGGATT
TGGCCAACTGAAAGTCCCGA

Downstream 100 bases:

>100_bases
GATGGGGTTCTTCACGTCACGATTGTTCTGGATCGCCCTGCTGGTGGTCGCTGTCGCGGCGACGCTGCCGCTCTACGTCT
CCGGCTACATCCTCGGGCTG

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MLALQILIDGFAISALYALGATGFTLIFGVSGVLNLSHGAIMVAAAVAAWAASSTFGIGSYAGALIGIATALVIALLTYF
AVVQPIQRSRRIANEEKEIFVLTGTLLWGIMIQEAIAYFFTNNAKTVLPIVTGVVEILGVRTPRNEIFTALVCCLVIALL
WLLVNRTKIGKAVLAASMNPRGVTLLGLELTNIYVVVWIIYGVLAGIAGVLLGMFLGVSSYSVGPLTASAFSIVVLGGLG
SVSGSLIAAFVVGYLETFTAYMISPAYRSIPALLLLVTVMYLRPQGLLGRR

Sequences:

>Translated_291_residues
MLALQILIDGFAISALYALGATGFTLIFGVSGVLNLSHGAIMVAAAVAAWAASSTFGIGSYAGALIGIATALVIALLTYF
AVVQPIQRSRRIANEEKEIFVLTGTLLWGIMIQEAIAYFFTNNAKTVLPIVTGVVEILGVRTPRNEIFTALVCCLVIALL
WLLVNRTKIGKAVLAASMNPRGVTLLGLELTNIYVVVWIIYGVLAGIAGVLLGMFLGVSSYSVGPLTASAFSIVVLGGLG
SVSGSLIAAFVVGYLETFTAYMISPAYRSIPALLLLVTVMYLRPQGLLGRR
>Mature_291_residues
MLALQILIDGFAISALYALGATGFTLIFGVSGVLNLSHGAIMVAAAVAAWAASSTFGIGSYAGALIGIATALVIALLTYF
AVVQPIQRSRRIANEEKEIFVLTGTLLWGIMIQEAIAYFFTNNAKTVLPIVTGVVEILGVRTPRNEIFTALVCCLVIALL
WLLVNRTKIGKAVLAASMNPRGVTLLGLELTNIYVVVWIIYGVLAGIAGVLLGMFLGVSSYSVGPLTASAFSIVVLGGLG
SVSGSLIAAFVVGYLETFTAYMISPAYRSIPALLLLVTVMYLRPQGLLGRR

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=297, Percent_Identity=26.9360269360269, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 30592; Mature: 30592

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLALQILIDGFAISALYALGATGFTLIFGVSGVLNLSHGAIMVAAAVAAWAASSTFGIGS
CEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHH
YAGALIGIATALVIALLTYFAVVQPIQRSRRIANEEKEIFVLTGTLLWGIMIQEAIAYFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHH
TNNAKTVLPIVTGVVEILGVRTPRNEIFTALVCCLVIALLWLLVNRTKIGKAVLAASMNP
HCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RGVTLLGLELTNIYVVVWIIYGVLAGIAGVLLGMFLGVSSYSVGPLTASAFSIVVLGGLG
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCC
SVSGSLIAAFVVGYLETFTAYMISPAYRSIPALLLLVTVMYLRPQGLLGRR
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLALQILIDGFAISALYALGATGFTLIFGVSGVLNLSHGAIMVAAAVAAWAASSTFGIGS
CEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHH
YAGALIGIATALVIALLTYFAVVQPIQRSRRIANEEKEIFVLTGTLLWGIMIQEAIAYFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHH
TNNAKTVLPIVTGVVEILGVRTPRNEIFTALVCCLVIALLWLLVNRTKIGKAVLAASMNP
HCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RGVTLLGLELTNIYVVVWIIYGVLAGIAGVLLGMFLGVSSYSVGPLTASAFSIVVLGGLG
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCC
SVSGSLIAAFVVGYLETFTAYMISPAYRSIPALLLLVTVMYLRPQGLLGRR
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]