Click here to switch to the map view.

The map label for this gene is 192290327

Identifier: 192290327

GI number: 192290327

Start: 2085151

End: 2086584

Strand: Reverse

Name: 192290327

Synonym: Rpal_1934

Alternate gene names: NA

Gene position: 2086584-2085151 (Counterclockwise)

Preceding gene: 192290329

Following gene: 192290326

Centisome position: 36.33

GC content: 67.78

Gene sequence:

>1434_bases
GTGGCGGCAGATCAGGCGCTGGTAAAGGAAACCGCTTGTGCAGTGGTGGGCAAGCTCAAGTCCGGCGAGGTGACGCCGAT
CGAGGTGCTCGACGTGCTCGAAGAGCGGATTGCCGAAGTCGACGGCGCCATCAATGCTCTCCCCACTCTGTGCTTCGATC
GTGCCCGCACCCACGCCGCCGAGCTGATGAAGAAACCGCCGGCCGAGCGCGGCCTTCTGGCCGGTCTGCCGGTGCCGATC
AAGGATCTCACCGCGGTCGCAGGCGTGCTCACCACCCAGGGCTCGCCGATCTACAAGGACAACGTCCCGGCGCGCTCCGA
CCTGATGGTCGAACATCTCGAAGCCAATGGCGGCGTGGTGTACGCCAAGTCGAACACCCCGGAATTCGGCGCCGGCGCCA
ATACGTTCAACGAAGTGTTCGGCGCGACCCGCAACCCGTGGGACCTATCGCGCTCGGCGGCGGGCTCGTCCGGCGGCGCC
GCGGCGGCGCTTGCATCGGGCACCGCATGGCTGGCCCACGGCTCGGACATGGGCGGCTCGCTGCGCAATCCGGCGAGCTT
CTGCGGCATCGTCGGTCTCCGTCCCAGCTTCGGCCGGGTGGCGCATACGCCGGCGGCGAAGATAGACCGCAATCTCGGCG
TTCAGGGCCCGATGGCCCGCAATGTCGAGGACGTCGCGCTGTTGCTCGACGCGATGAGCGGCGAATACGCCGCCGACCCG
CTGTCGATCCCCTCGCCTGCCACCTCGTTCCTCGCGGCGGCGCGGTCGCGGCAGAAGCCGCTGCGCGTGGCCTATTCGCC
CGATCTCGGCATCACTCCGGTCGATCCGGAAGTCGCCGCCGTCACCCGCAAGGCGGCCGAACGCTTCGCCGAGGCCGGCG
TGATCGTGGAACAGGCGCAGCCTGATCTGTCCGAAGCGCACGAGTGCTTCCAGGCGCTGCGCGCGTTCGACTTCGCGATC
AGCAAGGCCGATCTGCTGCGCACCAAGCGCGACCTGCTCAAACCGGAAGTGATCTGGAATATCGAACAGGGCCTCAAGCT
CAGCGTCGACGATCTGGCCAAGGCCGAAGCGCAGCGGGTGGCGATGACGGCGCGGACCATCGAGTTCTTCCGGACCTACG
ATCTGCTGCTGTGCCCAGCGACGATCACCGCGCCGTTCCCGGTCGAGCAGCGCTACCTCGCCGAATGCAACGGTCATACC
TTCGACAACTACGTCGAATGGCTCGGCATCGTCTACGCGATCACGCTGGTGTGCTGCCCTGCCCTGTCGCTGCCTTGCGG
CATCACCACGACCGGCCTGCCTGTCGGCCTGCAGATGGTGGCACGGCCGCGCAACGAAGCCGGCCTGCTCGCCGGCGCCG
CGGTGCTCGAGGATATCCTTGGCCTGAAGGGCACGACCCCGATCGACCCGCGTCCGGCGCCCGCCAGCCTGTAG

Upstream 100 bases:

>100_bases
GTCCGGGGTACCCATTGCGTCTAGTCTTGGTATCCGGTCCAAAAGCGCAGACGGTGATGTACTGATACCGCCGGCGCGAT
CAACTCAGGGGAGTTACAGG

Downstream 100 bases:

>100_bases
GCGCCTTCCGGGACTGGCGGTTGCAACACGCTGTGCTACTCTCACGCGTGTTGCAACACCGATAGATCCGCCGACAAAGG
CGGACGATAGGGAAACGCCA

Product: Amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MAADQALVKETACAVVGKLKSGEVTPIEVLDVLEERIAEVDGAINALPTLCFDRARTHAAELMKKPPAERGLLAGLPVPI
KDLTAVAGVLTTQGSPIYKDNVPARSDLMVEHLEANGGVVYAKSNTPEFGAGANTFNEVFGATRNPWDLSRSAAGSSGGA
AAALASGTAWLAHGSDMGGSLRNPASFCGIVGLRPSFGRVAHTPAAKIDRNLGVQGPMARNVEDVALLLDAMSGEYAADP
LSIPSPATSFLAAARSRQKPLRVAYSPDLGITPVDPEVAAVTRKAAERFAEAGVIVEQAQPDLSEAHECFQALRAFDFAI
SKADLLRTKRDLLKPEVIWNIEQGLKLSVDDLAKAEAQRVAMTARTIEFFRTYDLLLCPATITAPFPVEQRYLAECNGHT
FDNYVEWLGIVYAITLVCCPALSLPCGITTTGLPVGLQMVARPRNEAGLLAGAAVLEDILGLKGTTPIDPRPAPASL

Sequences:

>Translated_477_residues
MAADQALVKETACAVVGKLKSGEVTPIEVLDVLEERIAEVDGAINALPTLCFDRARTHAAELMKKPPAERGLLAGLPVPI
KDLTAVAGVLTTQGSPIYKDNVPARSDLMVEHLEANGGVVYAKSNTPEFGAGANTFNEVFGATRNPWDLSRSAAGSSGGA
AAALASGTAWLAHGSDMGGSLRNPASFCGIVGLRPSFGRVAHTPAAKIDRNLGVQGPMARNVEDVALLLDAMSGEYAADP
LSIPSPATSFLAAARSRQKPLRVAYSPDLGITPVDPEVAAVTRKAAERFAEAGVIVEQAQPDLSEAHECFQALRAFDFAI
SKADLLRTKRDLLKPEVIWNIEQGLKLSVDDLAKAEAQRVAMTARTIEFFRTYDLLLCPATITAPFPVEQRYLAECNGHT
FDNYVEWLGIVYAITLVCCPALSLPCGITTTGLPVGLQMVARPRNEAGLLAGAAVLEDILGLKGTTPIDPRPAPASL
>Mature_476_residues
AADQALVKETACAVVGKLKSGEVTPIEVLDVLEERIAEVDGAINALPTLCFDRARTHAAELMKKPPAERGLLAGLPVPIK
DLTAVAGVLTTQGSPIYKDNVPARSDLMVEHLEANGGVVYAKSNTPEFGAGANTFNEVFGATRNPWDLSRSAAGSSGGAA
AALASGTAWLAHGSDMGGSLRNPASFCGIVGLRPSFGRVAHTPAAKIDRNLGVQGPMARNVEDVALLLDAMSGEYAADPL
SIPSPATSFLAAARSRQKPLRVAYSPDLGITPVDPEVAAVTRKAAERFAEAGVIVEQAQPDLSEAHECFQALRAFDFAIS
KADLLRTKRDLLKPEVIWNIEQGLKLSVDDLAKAEAQRVAMTARTIEFFRTYDLLLCPATITAPFPVEQRYLAECNGHTF
DNYVEWLGIVYAITLVCCPALSLPCGITTTGLPVGLQMVARPRNEAGLLAGAAVLEDILGLKGTTPIDPRPAPASL

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=492, Percent_Identity=25.2032520325203, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI195972892, Length=490, Percent_Identity=24.2857142857143, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI166795287, Length=258, Percent_Identity=36.046511627907, Blast_Score=95, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17537465, Length=504, Percent_Identity=27.5793650793651, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71990152, Length=298, Percent_Identity=29.1946308724832, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17538252, Length=337, Percent_Identity=27.5964391691395, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17556264, Length=293, Percent_Identity=30.3754266211604, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17543272, Length=453, Percent_Identity=26.0485651214128, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17538254, Length=413, Percent_Identity=25.181598062954, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=310, Percent_Identity=30, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556278, Length=310, Percent_Identity=30, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320448, Length=237, Percent_Identity=29.1139240506329, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6319685, Length=432, Percent_Identity=23.6111111111111, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI45550774, Length=230, Percent_Identity=36.0869565217391, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24648435, Length=230, Percent_Identity=36.0869565217391, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24648437, Length=230, Percent_Identity=36.0869565217391, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24644968, Length=504, Percent_Identity=25.7936507936508, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21356731, Length=489, Percent_Identity=25.5623721881391, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24648441, Length=415, Percent_Identity=27.2289156626506, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24648439, Length=415, Percent_Identity=27.2289156626506, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI161078093, Length=497, Percent_Identity=26.1569416498994, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648113, Length=502, Percent_Identity=27.0916334661355, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24652985, Length=253, Percent_Identity=32.4110671936759, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19922090, Length=253, Percent_Identity=32.4110671936759, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24652981, Length=253, Percent_Identity=32.4110671936759, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24652983, Length=253, Percent_Identity=32.4110671936759, Blast_Score=105, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 50340; Mature: 50209

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAADQALVKETACAVVGKLKSGEVTPIEVLDVLEERIAEVDGAINALPTLCFDRARTHAA
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELMKKPPAERGLLAGLPVPIKDLTAVAGVLTTQGSPIYKDNVPARSDLMVEHLEANGGVV
HHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEE
YAKSNTPEFGAGANTFNEVFGATRNPWDLSRSAAGSSGGAAAALASGTAWLAHGSDMGGS
EECCCCCCCCCCCHHHHHHHCCCCCCCCCHHHCCCCCCCCHHHHHCCCEEEECCCCCCCC
LRNPASFCGIVGLRPSFGRVAHTPAAKIDRNLGVQGPMARNVEDVALLLDAMSGEYAADP
CCCCHHHHHHCCCCCCCCCHHCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
LSIPSPATSFLAAARSRQKPLRVAYSPDLGITPVDPEVAAVTRKAAERFAEAGVIVEQAQ
CCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCC
PDLSEAHECFQALRAFDFAISKADLLRTKRDLLKPEVIWNIEQGLKLSVDDLAKAEAQRV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCEECHHHHHHHHHHHH
AMTARTIEFFRTYDLLLCPATITAPFPVEQRYLAECNGHTFDNYVEWLGIVYAITLVCCP
HHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ALSLPCGITTTGLPVGLQMVARPRNEAGLLAGAAVLEDILGLKGTTPIDPRPAPASL
HCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AADQALVKETACAVVGKLKSGEVTPIEVLDVLEERIAEVDGAINALPTLCFDRARTHAA
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELMKKPPAERGLLAGLPVPIKDLTAVAGVLTTQGSPIYKDNVPARSDLMVEHLEANGGVV
HHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEE
YAKSNTPEFGAGANTFNEVFGATRNPWDLSRSAAGSSGGAAAALASGTAWLAHGSDMGGS
EECCCCCCCCCCCHHHHHHHCCCCCCCCCHHHCCCCCCCCHHHHHCCCEEEECCCCCCCC
LRNPASFCGIVGLRPSFGRVAHTPAAKIDRNLGVQGPMARNVEDVALLLDAMSGEYAADP
CCCCHHHHHHCCCCCCCCCHHCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
LSIPSPATSFLAAARSRQKPLRVAYSPDLGITPVDPEVAAVTRKAAERFAEAGVIVEQAQ
CCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCC
PDLSEAHECFQALRAFDFAISKADLLRTKRDLLKPEVIWNIEQGLKLSVDDLAKAEAQRV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCEECHHHHHHHHHHHH
AMTARTIEFFRTYDLLLCPATITAPFPVEQRYLAECNGHTFDNYVEWLGIVYAITLVCCP
HHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ALSLPCGITTTGLPVGLQMVARPRNEAGLLAGAAVLEDILGLKGTTPIDPRPAPASL
HCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]