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The map label for this gene is alkJ [H]

Identifier: 192290278

GI number: 192290278

Start: 2029247

End: 2030851

Strand: Reverse

Name: alkJ [H]

Synonym: Rpal_1882

Alternate gene names: 192290278

Gene position: 2030851-2029247 (Counterclockwise)

Preceding gene: 192290279

Following gene: 192290275

Centisome position: 35.36

GC content: 65.61

Gene sequence:

>1605_bases
GTGAGCGAAACAGTGGATTTCGTCGTGGTCGGCGGCGGCTCCGGCGGCTGCGCCGTCGCCGGTCGCTTGTCGGAAGACAC
CAACACGTCGGTGGCTCTGCTCGAAGCCGGCGGCACGGCCGACAACTGGGTGGTGAAGACGCCCTACGCGCTGTCGCTGA
TGGTGCCGAGCAAGCTGAACAACTGGCATTTCGAGACGGTGCCGCAGCCCGGCCTCAACGGCCGCGTCGGCTATCAGCCG
CGCGGCAAAGGGCTTGGCGGCTCGTCGGCGATCAACGCCATGGTCTATATCCGCGGCCACAAATGGGACTACGACCATTG
GGCCGAACTCGGCAATGCCGGCTGGTCCTACGCCGATGTGCTGCCGTACTTCAAGCGCTCGGAAAACAACTCCGATTTCA
ACGGCGCTTATCACGGCCAGAGCGGACCATTGCACGTCAACAAGCTGCGCACCGACAATCCGGCACACGAGATCTTCCTG
CAGGCGGCGCGCGAGGCGCAGTTTCGCATCCGTGACGACTTCAACGGCGAGGAGCAGGAAGGCCTCGGCTTGTATCAACT
CACCCAGCACGATGGCGAACGCTGGAGCGCCGCGCGCGCCTATGTGCATCCGCACATCGGGACGCGCTGCAATCTGCGGG
TCGAAACCGACGCGCAGGCGACCCGGATTCTGATCGAAGGCGGTCGCGCGGTCGGCGTCGCGTATCGGCAGAACGATCAG
ATCCGCGAGATCCGCGCGCGCCGCGAAGTCATCCTCGCGTCCGGCGCCTTCCAGTCGCCGCAGCTGCTGATGCTGTCGGG
CATCGGCGATGCCGCCGCGCTGAAGGCGCACGGCATCGACGTGGTGCACCATCTGCCGGGCGTCGGCCAGAACCTGCAGG
ATCACCCGGACTTCATCTTCGCCTATCAGTCGGACAGCCCGTATTTCACCGGCACCAGCTTCACCGGGATCGCCCGGCTG
CTGTCGACGATCGGTCAATATCGCCGCGAGGGCCGCGGACCGCTGACCACCAACTTCGCCGAATGCGGTGGCTTCCTGAA
GACACGTCCCGACGTCGACGTGCCGGATATCCAGCTGCATTTCGGCATGGCGATGGTCGACGATCACGGCCGCAAGCGGC
ACTGGGGCACGGGATTTTCCTGCCATTTCTGTCTGCTGCGGCCGAAGAGCCGCGGCAGCGTCGGGCTCGCCAGCGCCGAT
CCCTTGGCGCCGCCGCGGATCGATCCGAACTTCTTCGGCGATCCGGACGACCTCGAAACCATGGTGGCGGGCTACAAGAC
GACACAGCGGCTGATGGAGACGCCGGCGCTACGCGCCCTGCAGCAGAAGGATCTGTTCACCGCCAATGTCCGCACCGACG
ACGACATCCGCGCCATCCTGCGCGCCCGCGTCGACACGGTGTATCACCCGGTCGGCACCTGCAAGATGGGCAGCGACGCG
ACGGCGGTAGTCGATCCGCGCCTGAACGTGCATGGCATCGGGGCCTTGCGCATCGTCGACGCCTCGGTGATGCCGACTTT
GATCGGCGGCAACACCAACGCCCCCACCATCATGATCGGCGAGAAAGCCGCCGACATGATCCGCGAGGAGATGCGGGCGA
ACTGA

Upstream 100 bases:

>100_bases
CTGTCGAAGGTGCTGCGCCTGATGTCGTAACAAGAGAAGACCCGCACGGCCGAAGATGCCGGCGGCAATCAACAAGACAA
CATCGCGGAGGGAAACGCTC

Downstream 100 bases:

>100_bases
CGTCGTCCGCGTCACGTCAAGCCGACGCTTCCGGACGCACCACAAGATGCGCCGGAGCGACCGTCTTTGGATTCAGCCCC
TCTGCCCGATGAGGTGGACG

Product: glucose-methanol-choline oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MSETVDFVVVGGGSGGCAVAGRLSEDTNTSVALLEAGGTADNWVVKTPYALSLMVPSKLNNWHFETVPQPGLNGRVGYQP
RGKGLGGSSAINAMVYIRGHKWDYDHWAELGNAGWSYADVLPYFKRSENNSDFNGAYHGQSGPLHVNKLRTDNPAHEIFL
QAAREAQFRIRDDFNGEEQEGLGLYQLTQHDGERWSAARAYVHPHIGTRCNLRVETDAQATRILIEGGRAVGVAYRQNDQ
IREIRARREVILASGAFQSPQLLMLSGIGDAAALKAHGIDVVHHLPGVGQNLQDHPDFIFAYQSDSPYFTGTSFTGIARL
LSTIGQYRREGRGPLTTNFAECGGFLKTRPDVDVPDIQLHFGMAMVDDHGRKRHWGTGFSCHFCLLRPKSRGSVGLASAD
PLAPPRIDPNFFGDPDDLETMVAGYKTTQRLMETPALRALQQKDLFTANVRTDDDIRAILRARVDTVYHPVGTCKMGSDA
TAVVDPRLNVHGIGALRIVDASVMPTLIGGNTNAPTIMIGEKAADMIREEMRAN

Sequences:

>Translated_534_residues
MSETVDFVVVGGGSGGCAVAGRLSEDTNTSVALLEAGGTADNWVVKTPYALSLMVPSKLNNWHFETVPQPGLNGRVGYQP
RGKGLGGSSAINAMVYIRGHKWDYDHWAELGNAGWSYADVLPYFKRSENNSDFNGAYHGQSGPLHVNKLRTDNPAHEIFL
QAAREAQFRIRDDFNGEEQEGLGLYQLTQHDGERWSAARAYVHPHIGTRCNLRVETDAQATRILIEGGRAVGVAYRQNDQ
IREIRARREVILASGAFQSPQLLMLSGIGDAAALKAHGIDVVHHLPGVGQNLQDHPDFIFAYQSDSPYFTGTSFTGIARL
LSTIGQYRREGRGPLTTNFAECGGFLKTRPDVDVPDIQLHFGMAMVDDHGRKRHWGTGFSCHFCLLRPKSRGSVGLASAD
PLAPPRIDPNFFGDPDDLETMVAGYKTTQRLMETPALRALQQKDLFTANVRTDDDIRAILRARVDTVYHPVGTCKMGSDA
TAVVDPRLNVHGIGALRIVDASVMPTLIGGNTNAPTIMIGEKAADMIREEMRAN
>Mature_533_residues
SETVDFVVVGGGSGGCAVAGRLSEDTNTSVALLEAGGTADNWVVKTPYALSLMVPSKLNNWHFETVPQPGLNGRVGYQPR
GKGLGGSSAINAMVYIRGHKWDYDHWAELGNAGWSYADVLPYFKRSENNSDFNGAYHGQSGPLHVNKLRTDNPAHEIFLQ
AAREAQFRIRDDFNGEEQEGLGLYQLTQHDGERWSAARAYVHPHIGTRCNLRVETDAQATRILIEGGRAVGVAYRQNDQI
REIRARREVILASGAFQSPQLLMLSGIGDAAALKAHGIDVVHHLPGVGQNLQDHPDFIFAYQSDSPYFTGTSFTGIARLL
STIGQYRREGRGPLTTNFAECGGFLKTRPDVDVPDIQLHFGMAMVDDHGRKRHWGTGFSCHFCLLRPKSRGSVGLASADP
LAPPRIDPNFFGDPDDLETMVAGYKTTQRLMETPALRALQQKDLFTANVRTDDDIRAILRARVDTVYHPVGTCKMGSDAT
AVVDPRLNVHGIGALRIVDASVMPTLIGGNTNAPTIMIGEKAADMIREEMRAN

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=540, Percent_Identity=44.4444444444444, Blast_Score=427, Evalue=1e-119,
Organism=Escherichia coli, GI1786503, Length=532, Percent_Identity=40.0375939849624, Blast_Score=358, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17532301, Length=545, Percent_Identity=38.7155963302752, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24642055, Length=576, Percent_Identity=37.8472222222222, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24642042, Length=577, Percent_Identity=38.9948006932409, Blast_Score=336, Evalue=3e-92,
Organism=Drosophila melanogaster, GI45551458, Length=568, Percent_Identity=38.9084507042254, Blast_Score=334, Evalue=1e-91,
Organism=Drosophila melanogaster, GI45549471, Length=568, Percent_Identity=38.9084507042254, Blast_Score=334, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24642037, Length=577, Percent_Identity=38.6481802426343, Blast_Score=328, Evalue=4e-90,
Organism=Drosophila melanogaster, GI24642048, Length=569, Percent_Identity=37.9613356766257, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24642059, Length=571, Percent_Identity=37.1278458844133, Blast_Score=319, Evalue=3e-87,
Organism=Drosophila melanogaster, GI24642035, Length=579, Percent_Identity=38.1692573402418, Blast_Score=318, Evalue=5e-87,
Organism=Drosophila melanogaster, GI24650267, Length=571, Percent_Identity=36.9527145359019, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI18859995, Length=568, Percent_Identity=37.3239436619718, Blast_Score=317, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24642039, Length=570, Percent_Identity=37.0175438596491, Blast_Score=312, Evalue=3e-85,
Organism=Drosophila melanogaster, GI17137792, Length=553, Percent_Identity=35.9855334538879, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI18859993, Length=568, Percent_Identity=33.4507042253521, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24642051, Length=574, Percent_Identity=32.9268292682927, Blast_Score=260, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24645930, Length=584, Percent_Identity=31.6780821917808, Blast_Score=193, Evalue=3e-49,
Organism=Drosophila melanogaster, GI24642057, Length=584, Percent_Identity=30.3082191780822, Blast_Score=161, Evalue=9e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 58389; Mature: 58258

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETVDFVVVGGGSGGCAVAGRLSEDTNTSVALLEAGGTADNWVVKTPYALSLMVPSKLN
CCCCEEEEEEECCCCCEEEEEECCCCCCCEEEEEECCCCCCCCEEECCEEEEEEECCCCC
NWHFETVPQPGLNGRVGYQPRGKGLGGSSAINAMVYIRGHKWDYDHWAELGNAGWSYADV
CCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHCCCCCCHHHH
LPYFKRSENNSDFNGAYHGQSGPLHVNKLRTDNPAHEIFLQAAREAQFRIRDDFNGEEQE
HHHHHCCCCCCCCCCEECCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCHHH
GLGLYQLTQHDGERWSAARAYVHPHIGTRCNLRVETDAQATRILIEGGRAVGVAYRQNDQ
CCCEEEEECCCCCHHHHHHEEECCCCCCEEEEEEECCCCEEEEEEECCCEEEEEECCCCH
IREIRARREVILASGAFQSPQLLMLSGIGDAAALKAHGIDVVHHLPGVGQNLQDHPDFIF
HHHHHHHHHEEEECCCCCCCCEEEEECCCCHHHHHHCCCHHHHHCCCCCCCCCCCCCEEE
AYQSDSPYFTGTSFTGIARLLSTIGQYRREGRGPLTTNFAECGGFLKTRPDVDVPDIQLH
EEECCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEE
FGMAMVDDHGRKRHWGTGFSCHFCLLRPKSRGSVGLASADPLAPPRIDPNFFGDPDDLET
ECEEEECCCCCCCCCCCCCEEEEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHH
MVAGYKTTQRLMETPALRALQQKDLFTANVRTDDDIRAILRARVDTVYHPVGTCKMGSDA
HHHHHHHHHHHHHCHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCEECCCCC
TAVVDPRLNVHGIGALRIVDASVMPTLIGGNTNAPTIMIGEKAADMIREEMRAN
EEEECCCCCEEECEEEEEECCCCCCEEECCCCCCCEEEECHHHHHHHHHHHHCC
>Mature Secondary Structure 
SETVDFVVVGGGSGGCAVAGRLSEDTNTSVALLEAGGTADNWVVKTPYALSLMVPSKLN
CCCEEEEEEECCCCCEEEEEECCCCCCCEEEEEECCCCCCCCEEECCEEEEEEECCCCC
NWHFETVPQPGLNGRVGYQPRGKGLGGSSAINAMVYIRGHKWDYDHWAELGNAGWSYADV
CCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHCCCCCCHHHH
LPYFKRSENNSDFNGAYHGQSGPLHVNKLRTDNPAHEIFLQAAREAQFRIRDDFNGEEQE
HHHHHCCCCCCCCCCEECCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCHHH
GLGLYQLTQHDGERWSAARAYVHPHIGTRCNLRVETDAQATRILIEGGRAVGVAYRQNDQ
CCCEEEEECCCCCHHHHHHEEECCCCCCEEEEEEECCCCEEEEEEECCCEEEEEECCCCH
IREIRARREVILASGAFQSPQLLMLSGIGDAAALKAHGIDVVHHLPGVGQNLQDHPDFIF
HHHHHHHHHEEEECCCCCCCCEEEEECCCCHHHHHHCCCHHHHHCCCCCCCCCCCCCEEE
AYQSDSPYFTGTSFTGIARLLSTIGQYRREGRGPLTTNFAECGGFLKTRPDVDVPDIQLH
EEECCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEE
FGMAMVDDHGRKRHWGTGFSCHFCLLRPKSRGSVGLASADPLAPPRIDPNFFGDPDDLET
ECEEEECCCCCCCCCCCCCEEEEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHH
MVAGYKTTQRLMETPALRALQQKDLFTANVRTDDDIRAILRARVDTVYHPVGTCKMGSDA
HHHHHHHHHHHHHCHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCEECCCCC
TAVVDPRLNVHGIGALRIVDASVMPTLIGGNTNAPTIMIGEKAADMIREEMRAN
EEEECCCCCEEECEEEEEECCCCCCEEECCCCCCCEEEECHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]