Click here to switch to the map view.

The map label for this gene is ltaA [H]

Identifier: 192290174

GI number: 192290174

Start: 1910386

End: 1911468

Strand: Reverse

Name: ltaA [H]

Synonym: Rpal_1776

Alternate gene names: 192290174

Gene position: 1911468-1910386 (Counterclockwise)

Preceding gene: 192290175

Following gene: 192290170

Centisome position: 33.28

GC content: 68.42

Gene sequence:

>1083_bases
ATGTTAGCCTCGGGTATGGCCTACATCCCTTCCCTACCCGATCCGAACGCGCCGCCTGTGCGGATCAATCTGCTCTCCGA
CACCCAGACCCGGCCGACGCCGGCGATGCGCGAGGCGATGGCGCGGGCCGAGGTCGGCGACGAGCAGACCGGCGACGATC
CGACCACCAACCTGTTGCAGGAGCGCGTCGCTGCGCTGCTCGGCAAACAGGCGGCGGTGTTCCTGCCCTCGGGCACGATG
TGCAACGTCACGACCACGCTCGCGACCTGCCGGCCTGGCGACGAGATCATCGCCCATCGCACCGCGCATATCCTGTCGCG
CGAAGGCGGCGCTCACGCTGCGCTCGGCGGCTTTCAGATCACCGGCCTAGACGGCGACGACGGCCAGTTCTCACTCGAGG
CGTTCCGCGCCGCGCTGCATCCGAAGACGCGCTACGAACCGCCGCAAGTGATGGTCAGCGCCGAACAGACCGCCAATATC
GGCGGCGGCACGATCTGGCCGCAGGCCACGCTCGACGCGGTCGCCGCTGCCGGCAAGGACGCCGGGCTGTGGACGCATAT
CGACGGCGCACGGCTGATGAACGCGGTGGTCGCCACGGGTATCAGTGCCAAAGCGATGACGGCAGGCTGGGATTCGGCGT
GGATCGATTTCAGCAAGGGCCTCGGTGCACCGGTCGGCGCCGTGCTGGCCGGCCCGCGTGCCTTCATCAACGAGGTGTGG
CGCTGGAAGCAGCGGCTCGGCGGCGCGATGCGGCAGTCCGGCGTGATTGCCGCGGCGTGTCTGTACGCGCTCGATCATCA
CGTCGATCGCCTTGCCGACGATCATGCCAACGCGCGCCGTCTTGCCTCCGGGCTGGCGCAGATCGACGGCATCGAGGTGC
AGACGCCGCAGACCAATCTGGTGTTCTTCAAACCGGACCGCACCGGCGTCTCCGGCGCCGACATGGTCGCACAGCTCCGC
CGGCACGGCGTGGTGCTGGCGATGATGGAGGGACGGATCCGCGCCTGCACGCATCTCGACGTCAGCGCGGACATGATCGA
GCAAACGCTCGACCACGTCCGCGAGATCGTGCGGGCAGCCTGA

Upstream 100 bases:

>100_bases
CTGACGCGAGCGCCCCGGAATGACGGCCTTGGCGATGGTACCAATCACATCCCGCTGACCTGATCGTGTCCGGGCAAAAC
TCTTCCGCTTCGCACCCGGC

Downstream 100 bases:

>100_bases
CCTTTACAGGCAGATCCGGACGCCGGCGGCGTTGACGACGCAGCGGCCGCCGTAAGACCCGCGTCCCCGTCCACGGTCCC
AGCCGCGACCGCGACCCCGG

Product: aromatic amino acid beta-eliminating lyase/threonine aldolase

Products: Acetaldehyde; Glycine [C]

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MLASGMAYIPSLPDPNAPPVRINLLSDTQTRPTPAMREAMARAEVGDEQTGDDPTTNLLQERVAALLGKQAAVFLPSGTM
CNVTTTLATCRPGDEIIAHRTAHILSREGGAHAALGGFQITGLDGDDGQFSLEAFRAALHPKTRYEPPQVMVSAEQTANI
GGGTIWPQATLDAVAAAGKDAGLWTHIDGARLMNAVVATGISAKAMTAGWDSAWIDFSKGLGAPVGAVLAGPRAFINEVW
RWKQRLGGAMRQSGVIAAACLYALDHHVDRLADDHANARRLASGLAQIDGIEVQTPQTNLVFFKPDRTGVSGADMVAQLR
RHGVVLAMMEGRIRACTHLDVSADMIEQTLDHVREIVRAA

Sequences:

>Translated_360_residues
MLASGMAYIPSLPDPNAPPVRINLLSDTQTRPTPAMREAMARAEVGDEQTGDDPTTNLLQERVAALLGKQAAVFLPSGTM
CNVTTTLATCRPGDEIIAHRTAHILSREGGAHAALGGFQITGLDGDDGQFSLEAFRAALHPKTRYEPPQVMVSAEQTANI
GGGTIWPQATLDAVAAAGKDAGLWTHIDGARLMNAVVATGISAKAMTAGWDSAWIDFSKGLGAPVGAVLAGPRAFINEVW
RWKQRLGGAMRQSGVIAAACLYALDHHVDRLADDHANARRLASGLAQIDGIEVQTPQTNLVFFKPDRTGVSGADMVAQLR
RHGVVLAMMEGRIRACTHLDVSADMIEQTLDHVREIVRAA
>Mature_360_residues
MLASGMAYIPSLPDPNAPPVRINLLSDTQTRPTPAMREAMARAEVGDEQTGDDPTTNLLQERVAALLGKQAAVFLPSGTM
CNVTTTLATCRPGDEIIAHRTAHILSREGGAHAALGGFQITGLDGDDGQFSLEAFRAALHPKTRYEPPQVMVSAEQTANI
GGGTIWPQATLDAVAAAGKDAGLWTHIDGARLMNAVVATGISAKAMTAGWDSAWIDFSKGLGAPVGAVLAGPRAFINEVW
RWKQRLGGAMRQSGVIAAACLYALDHHVDRLADDHANARRLASGLAQIDGIEVQTPQTNLVFFKPDRTGVSGADMVAQLR
RHGVVLAMMEGRIRACTHLDVSADMIEQTLDHVREIVRAA

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=337, Percent_Identity=39.7626112759644, Blast_Score=216, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI193206522, Length=353, Percent_Identity=34.5609065155807, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI193206524, Length=352, Percent_Identity=34.6590909090909, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI193206520, Length=353, Percent_Identity=34.5609065155807, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6320789, Length=342, Percent_Identity=30.1169590643275, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI21355439, Length=311, Percent_Identity=38.9067524115756, Blast_Score=210, Evalue=9e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5 [C]

Molecular weight: Translated: 38217; Mature: 38217

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLASGMAYIPSLPDPNAPPVRINLLSDTQTRPTPAMREAMARAEVGDEQTGDDPTTNLLQ
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHH
ERVAALLGKQAAVFLPSGTMCNVTTTLATCRPGDEIIAHRTAHILSREGGAHAALGGFQI
HHHHHHHCCCCEEEECCCCEEEEEHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEECCEEE
TGLDGDDGQFSLEAFRAALHPKTRYEPPQVMVSAEQTANIGGGTIWPQATLDAVAAAGKD
ECCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECHHHCCCCCCCCCCHHHHHHHHHCCCC
AGLWTHIDGARLMNAVVATGISAKAMTAGWDSAWIDFSKGLGAPVGAVLAGPRAFINEVW
CCCEEECCHHHHHHHHHHCCCCHHHEECCCCHHHHHHHCCCCCCHHHHHCCCHHHHHHHH
RWKQRLGGAMRQSGVIAAACLYALDHHVDRLADDHANARRLASGLAQIDGIEVQTPQTNL
HHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEECCCCCE
VFFKPDRTGVSGADMVAQLRRHGVVLAMMEGRIRACTHLDVSADMIEQTLDHVREIVRAA
EEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLASGMAYIPSLPDPNAPPVRINLLSDTQTRPTPAMREAMARAEVGDEQTGDDPTTNLLQ
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHH
ERVAALLGKQAAVFLPSGTMCNVTTTLATCRPGDEIIAHRTAHILSREGGAHAALGGFQI
HHHHHHHCCCCEEEECCCCEEEEEHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEECCEEE
TGLDGDDGQFSLEAFRAALHPKTRYEPPQVMVSAEQTANIGGGTIWPQATLDAVAAAGKD
ECCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECHHHCCCCCCCCCCHHHHHHHHHCCCC
AGLWTHIDGARLMNAVVATGISAKAMTAGWDSAWIDFSKGLGAPVGAVLAGPRAFINEVW
CCCEEECCHHHHHHHHHHCCCCHHHEECCCCHHHHHHHCCCCCCHHHHHCCCHHHHHHHH
RWKQRLGGAMRQSGVIAAACLYALDHHVDRLADDHANARRLASGLAQIDGIEVQTPQTNL
HHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEECCCCCE
VFFKPDRTGVSGADMVAQLRRHGVVLAMMEGRIRACTHLDVSADMIEQTLDHVREIVRAA
EEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 0.22 {L-allo-Thr}} 2.85 {L-Thr}} 0.12 {DL-threo-phenylserine}} 0.24 {DL-erythro-phenylserine}} [C]

Substrates: L-Threonine [C]

Specific reaction: L-Threonine <==> Acetaldehyde + Glycine [C]

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]