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The map label for this gene is paaK [H]

Identifier: 192290012

GI number: 192290012

Start: 1727661

End: 1728998

Strand: Reverse

Name: paaK [H]

Synonym: Rpal_1613

Alternate gene names: 192290012

Gene position: 1728998-1727661 (Counterclockwise)

Preceding gene: 192290013

Following gene: 192290005

Centisome position: 30.1

GC content: 64.95

Gene sequence:

>1338_bases
ATGACCACGACTTCCGACGAGCGTCCGTATTGGGACGCCAAGCTCGAAACCCAATCGCGCGCCGACTGGGACGCGCTGAA
ACTGTCGCTGCTGCAGAAGCACATCGCCCACGCCTATGCGGGTTCGCCCGCCTATCGCGCCGCGTTCGACGCCGCCAAGG
TCTCGCCGGCTCAGATCAAATCGCTCGACGACATCCGCCGCTTTCCGTTCATCGACAAGCGCACCCTGCGCGACCGCCAG
CTGGCGGTGCCGCCGTTCGGTGACCTCGTCGCTGTGCCGGAGCGCGACATCGTCTACATTTCGGCTTCCTCGGGCTCTAC
CGGCGTGCCGACCGCCTCACCGTTCACCCAATCCGATTTCGACGGCTGGATCGACTATGAGGCGCGGCAGTTTTGGTCGT
CCGGGATGCGGCCGAGCGATCGCTACGTGCATTCGCTGAATTTCTCGCTGTTCATCGGCGGCCCGTGCGTGCTCGGCGCG
CAGAAACTTGGCGCGCTGACGATTCACGCCGGCACAATTCCATCCGATCGGCTGCTGCAGATCCTCCGCCAGTTCCAGGC
CACGGTGATCTGGACCACGCCGTCGTACGCCTGGTACCTCGGTGAGACCGCCATCAAGGAAGGCTACGACTTGCGCAAGG
ATCTCGCCGTGCGCCGGATCTTCGTCGCCGGCGAGCCCGGCGGTTCGATTCCCGAAACCCGCAACCGCATCGAGCAGCTG
TGGGGCGCCTCGGTGTACGACTATTACGGCCTGTCCGACATCTTCGGCTCCTGCGCCGGGATGTGCGAGCAGAAAGAGGG
GCTGCACTGGGCCGAAGATCACATCCTTGTCGAAGTGATCGATCCCGACAGCGGCGAACCGGTGAAGCCGGGAGACCGCG
GCGAAATGGTGCTGACGACGCTGAAGAAGACGGCGCGGCCGATGATCCGGTTCCGCACCGGCGACATCGTCAGCTTCAAT
CCGGAGCCTTGCCGCTGCGGTCGCACCGCGATCCGGCTCAACGGCGTGCATGGCAGGCGCGACGACATGCTGATCATCAA
GGGTGTCAACCTGTTCCCGAGCGATGTCGAAGCGGTAGCGCGGCAGGATCATGAGCTGACCGGCGAGTATCGGCTGGTGG
TCGACCGCATCAACCACCTCGACCGCCTCATCGTCGAGGTCGAGCACATCCAAGGCTACAACGGCGAGACGCATGCGCTC
GCCGACCGGTTCGAACGCAAGCTGAAGGCCATCACAGGCGTCTCGGCCGCGGTCTCAGTGCTGAAGCCCGACACCCTGCC
GCGCGCCACGCACAAGGCCAAACGGGTCGAGGACCGGCGCAGCGGGGTGTGGAGCTGA

Upstream 100 bases:

>100_bases
GGTGAGACCGGCCGCCGCGACGACAATCAACACCATTTCAGTCATCGACAGCGCGACCGGCCGCCCAGCGGCCGGTCGTC
GGCATTCAGGAACACTGCAC

Downstream 100 bases:

>100_bases
AGAAGAGGACTGAGGACAAGGCGGCGGAGCGTCAGCGTTCCGCCGCAGCTCTTCACATCGTCATTGCGAGGAGCGGAGCG
ACGACAATCCAGCTTCGTGC

Product: Phenylacetate--CoA ligase

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MTTTSDERPYWDAKLETQSRADWDALKLSLLQKHIAHAYAGSPAYRAAFDAAKVSPAQIKSLDDIRRFPFIDKRTLRDRQ
LAVPPFGDLVAVPERDIVYISASSGSTGVPTASPFTQSDFDGWIDYEARQFWSSGMRPSDRYVHSLNFSLFIGGPCVLGA
QKLGALTIHAGTIPSDRLLQILRQFQATVIWTTPSYAWYLGETAIKEGYDLRKDLAVRRIFVAGEPGGSIPETRNRIEQL
WGASVYDYYGLSDIFGSCAGMCEQKEGLHWAEDHILVEVIDPDSGEPVKPGDRGEMVLTTLKKTARPMIRFRTGDIVSFN
PEPCRCGRTAIRLNGVHGRRDDMLIIKGVNLFPSDVEAVARQDHELTGEYRLVVDRINHLDRLIVEVEHIQGYNGETHAL
ADRFERKLKAITGVSAAVSVLKPDTLPRATHKAKRVEDRRSGVWS

Sequences:

>Translated_445_residues
MTTTSDERPYWDAKLETQSRADWDALKLSLLQKHIAHAYAGSPAYRAAFDAAKVSPAQIKSLDDIRRFPFIDKRTLRDRQ
LAVPPFGDLVAVPERDIVYISASSGSTGVPTASPFTQSDFDGWIDYEARQFWSSGMRPSDRYVHSLNFSLFIGGPCVLGA
QKLGALTIHAGTIPSDRLLQILRQFQATVIWTTPSYAWYLGETAIKEGYDLRKDLAVRRIFVAGEPGGSIPETRNRIEQL
WGASVYDYYGLSDIFGSCAGMCEQKEGLHWAEDHILVEVIDPDSGEPVKPGDRGEMVLTTLKKTARPMIRFRTGDIVSFN
PEPCRCGRTAIRLNGVHGRRDDMLIIKGVNLFPSDVEAVARQDHELTGEYRLVVDRINHLDRLIVEVEHIQGYNGETHAL
ADRFERKLKAITGVSAAVSVLKPDTLPRATHKAKRVEDRRSGVWS
>Mature_444_residues
TTTSDERPYWDAKLETQSRADWDALKLSLLQKHIAHAYAGSPAYRAAFDAAKVSPAQIKSLDDIRRFPFIDKRTLRDRQL
AVPPFGDLVAVPERDIVYISASSGSTGVPTASPFTQSDFDGWIDYEARQFWSSGMRPSDRYVHSLNFSLFIGGPCVLGAQ
KLGALTIHAGTIPSDRLLQILRQFQATVIWTTPSYAWYLGETAIKEGYDLRKDLAVRRIFVAGEPGGSIPETRNRIEQLW
GASVYDYYGLSDIFGSCAGMCEQKEGLHWAEDHILVEVIDPDSGEPVKPGDRGEMVLTTLKKTARPMIRFRTGDIVSFNP
EPCRCGRTAIRLNGVHGRRDDMLIIKGVNLFPSDVEAVARQDHELTGEYRLVVDRINHLDRLIVEVEHIQGYNGETHALA
DRFERKLKAITGVSAAVSVLKPDTLPRATHKAKRVEDRRSGVWS

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=436, Percent_Identity=36.0091743119266, Blast_Score=246, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 49769; Mature: 49638

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTSDERPYWDAKLETQSRADWDALKLSLLQKHIAHAYAGSPAYRAAFDAAKVSPAQIK
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHH
SLDDIRRFPFIDKRTLRDRQLAVPPFGDLVAVPERDIVYISASSGSTGVPTASPFTQSDF
HHHHHHHCCCCCCHHCCCCEECCCCCCCEEECCCCCEEEEECCCCCCCCCCCCCCCCCCC
DGWIDYEARQFWSSGMRPSDRYVHSLNFSLFIGGPCVLGAQKLGALTIHAGTIPSDRLLQ
CCCCCHHHHHHHHCCCCCCHHEEEEECEEEEECCCCEECHHHHCEEEEEECCCCHHHHHH
ILRQFQATVIWTTPSYAWYLGETAIKEGYDLRKDLAVRRIFVAGEPGGSIPETRNRIEQL
HHHHHCCEEEEECCCCEEECCHHHHHCCCHHHHHHHEEEEEEECCCCCCCCHHHHHHHHH
WGASVYDYYGLSDIFGSCAGMCEQKEGLHWAEDHILVEVIDPDSGEPVKPGDRGEMVLTT
HCCCHHHHCCHHHHHHHHHHHHHHHCCCEEECCCEEEEEECCCCCCCCCCCCCCCHHHHH
LKKTARPMIRFRTGDIVSFNPEPCRCGRTAIRLNGVHGRRDDMLIIKGVNLFPSDVEAVA
HHHHHCCEEEEECCCEEEECCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCHHHHHHH
RQDHELTGEYRLVVDRINHLDRLIVEVEHIQGYNGETHALADRFERKLKAITGVSAAVSV
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LKPDTLPRATHKAKRVEDRRSGVWS
HCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TTTSDERPYWDAKLETQSRADWDALKLSLLQKHIAHAYAGSPAYRAAFDAAKVSPAQIK
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHH
SLDDIRRFPFIDKRTLRDRQLAVPPFGDLVAVPERDIVYISASSGSTGVPTASPFTQSDF
HHHHHHHCCCCCCHHCCCCEECCCCCCCEEECCCCCEEEEECCCCCCCCCCCCCCCCCCC
DGWIDYEARQFWSSGMRPSDRYVHSLNFSLFIGGPCVLGAQKLGALTIHAGTIPSDRLLQ
CCCCCHHHHHHHHCCCCCCHHEEEEECEEEEECCCCEECHHHHCEEEEEECCCCHHHHHH
ILRQFQATVIWTTPSYAWYLGETAIKEGYDLRKDLAVRRIFVAGEPGGSIPETRNRIEQL
HHHHHCCEEEEECCCCEEECCHHHHHCCCHHHHHHHEEEEEEECCCCCCCCHHHHHHHHH
WGASVYDYYGLSDIFGSCAGMCEQKEGLHWAEDHILVEVIDPDSGEPVKPGDRGEMVLTT
HCCCHHHHCCHHHHHHHHHHHHHHHCCCEEECCCEEEEEECCCCCCCCCCCCCCCHHHHH
LKKTARPMIRFRTGDIVSFNPEPCRCGRTAIRLNGVHGRRDDMLIIKGVNLFPSDVEAVA
HHHHHCCEEEEECCCEEEECCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCHHHHHHH
RQDHELTGEYRLVVDRINHLDRLIVEVEHIQGYNGETHALADRFERKLKAITGVSAAVSV
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LKPDTLPRATHKAKRVEDRRSGVWS
HCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]