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The map label for this gene is paaF [C]

Identifier: 192289993

GI number: 192289993

Start: 1705199

End: 1705990

Strand: Reverse

Name: paaF [C]

Synonym: Rpal_1594

Alternate gene names: 192289993

Gene position: 1705990-1705199 (Counterclockwise)

Preceding gene: 192289995

Following gene: 192289992

Centisome position: 29.7

GC content: 66.67

Gene sequence:

>792_bases
ATGCCTGCACGCAGCGCAGCCGACGGCGATGCCGTCGTGTTCGAACGCCATACCGACGTCATGGTGGTTCGCCTCAACAG
CCCGTCGACGCGCAATGCGCTGCAACCAGCGGTCAAGCAGCATCTCGAACTCCGCATCCCGGAATTACTCACCGATCCAG
CCGTGCGCTGCCTGGTGATCACCGGTAGCGAGGACTCGTTCTGTGCCGGCGGTGATATCAACTACATGGGCGATCGCGGC
GCACCGCAGGTGCGGCGGCGGATGCAGGCCACGCACGGCTGGGCCAAGATGCTGCTGACAGCGGAGAAACCAGTGATCGC
CGCCGTCAACGGGTCGGCCGCTGGTGCCGGCTTCTCGCTGGCGCTGCTCTGCGACATCGTGCTGATGTCGGATCAAGCTT
ACTTCCGCGCGGCATTTCCCGGGCTCGGTGCCGCACCGGATCTCGGCTTGGCGCTGACGCTGCCACGGGCGGTCGGCGCG
ATGCGCGCCAAAGACATCCTGCTCACCAACCGCCGCGTCGAAGCGGAGGAGGCGCTCGCGCTCGGCATCGCCAAGCGCGT
CGTCCCGGCTGACACGCTGATGGCCGAGGCGCTGCAGCTCGCTGCTGATCTTGCCGCCGGTCCCGCCACCTCGCTCGGCC
TCACCAAGATGCTGGTCAACGGCGCGTTCGAGCCGATTGAACAGTTCTTCGCCCTCGAGGCCTTCGCCCAGGGCATCGCC
TTCGGCAGCGCCGAATTCGCCGAAGGCGTCGCCGCCTTTCAGGAAAAGCGGAAAGCGGACTTCAGGCGCTGA

Upstream 100 bases:

>100_bases
GTGTTGTTGCTTGAACGCGTTTTAGCATTGCGAAAATCGTTTTCGCAAATTGTTACGAACTGCGCTATCCTCGCCGGAAA
CAAAACAAGGACGGAAACAC

Downstream 100 bases:

>100_bases
CAAGCGTTCGCAGAAGATGGAGATGAGTTCGGCAGCACATCGTTGCAGATCGGTAAGCTGGTCATCGAGACCGCATGATC
GTACCGATTCCGCCGAGCCA

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MPARSAADGDAVVFERHTDVMVVRLNSPSTRNALQPAVKQHLELRIPELLTDPAVRCLVITGSEDSFCAGGDINYMGDRG
APQVRRRMQATHGWAKMLLTAEKPVIAAVNGSAAGAGFSLALLCDIVLMSDQAYFRAAFPGLGAAPDLGLALTLPRAVGA
MRAKDILLTNRRVEAEEALALGIAKRVVPADTLMAEALQLAADLAAGPATSLGLTKMLVNGAFEPIEQFFALEAFAQGIA
FGSAEFAEGVAAFQEKRKADFRR

Sequences:

>Translated_263_residues
MPARSAADGDAVVFERHTDVMVVRLNSPSTRNALQPAVKQHLELRIPELLTDPAVRCLVITGSEDSFCAGGDINYMGDRG
APQVRRRMQATHGWAKMLLTAEKPVIAAVNGSAAGAGFSLALLCDIVLMSDQAYFRAAFPGLGAAPDLGLALTLPRAVGA
MRAKDILLTNRRVEAEEALALGIAKRVVPADTLMAEALQLAADLAAGPATSLGLTKMLVNGAFEPIEQFFALEAFAQGIA
FGSAEFAEGVAAFQEKRKADFRR
>Mature_262_residues
PARSAADGDAVVFERHTDVMVVRLNSPSTRNALQPAVKQHLELRIPELLTDPAVRCLVITGSEDSFCAGGDINYMGDRGA
PQVRRRMQATHGWAKMLLTAEKPVIAAVNGSAAGAGFSLALLCDIVLMSDQAYFRAAFPGLGAAPDLGLALTLPRAVGAM
RAKDILLTNRRVEAEEALALGIAKRVVPADTLMAEALQLAADLAAGPATSLGLTKMLVNGAFEPIEQFFALEAFAQGIAF
GSAEFAEGVAAFQEKRKADFRR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=265, Percent_Identity=33.5849056603774, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI221136756, Length=257, Percent_Identity=24.5136186770428, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI221136753, Length=257, Percent_Identity=24.5136186770428, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI221307494, Length=257, Percent_Identity=24.5136186770428, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI116235487, Length=221, Percent_Identity=25.7918552036199, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI4757968, Length=201, Percent_Identity=26.865671641791, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI49355787, Length=201, Percent_Identity=26.865671641791, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI25453481, Length=201, Percent_Identity=25.8706467661692, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI51538573, Length=201, Percent_Identity=25.8706467661692, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI4757966, Length=158, Percent_Identity=27.8481012658228, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI51538581, Length=158, Percent_Identity=27.8481012658228, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI45643119, Length=220, Percent_Identity=24.0909090909091, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260275230, Length=220, Percent_Identity=24.0909090909091, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI70995211, Length=192, Percent_Identity=29.6875, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260274832, Length=220, Percent_Identity=24.0909090909091, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI31542718, Length=250, Percent_Identity=27.6, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI20127408, Length=175, Percent_Identity=30.2857142857143, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI68989263, Length=195, Percent_Identity=30.7692307692308, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1787659, Length=260, Percent_Identity=32.3076923076923, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1787660, Length=250, Percent_Identity=37.6, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1788597, Length=256, Percent_Identity=26.5625, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI221142681, Length=250, Percent_Identity=32, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI87082183, Length=248, Percent_Identity=25.4032258064516, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788682, Length=192, Percent_Identity=30.2083333333333, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17554946, Length=245, Percent_Identity=33.0612244897959, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI25145438, Length=240, Percent_Identity=34.1666666666667, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17535521, Length=255, Percent_Identity=25.8823529411765, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17560910, Length=244, Percent_Identity=27.0491803278689, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=228, Percent_Identity=28.0701754385965, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17558304, Length=182, Percent_Identity=30.2197802197802, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=218, Percent_Identity=28.4403669724771, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17540714, Length=219, Percent_Identity=26.4840182648402, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=193, Percent_Identity=27.4611398963731, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=215, Percent_Identity=28.3720930232558, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17508951, Length=180, Percent_Identity=28.3333333333333, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=180, Percent_Identity=28.3333333333333, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI25144276, Length=180, Percent_Identity=28.3333333333333, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI20129971, Length=246, Percent_Identity=35.7723577235772, Blast_Score=136, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24653477, Length=246, Percent_Identity=35.7723577235772, Blast_Score=136, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24654903, Length=253, Percent_Identity=26.8774703557312, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21357171, Length=262, Percent_Identity=27.4809160305344, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24650670, Length=214, Percent_Identity=29.4392523364486, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI19920382, Length=227, Percent_Identity=29.9559471365639, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24653139, Length=265, Percent_Identity=25.2830188679245, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24583077, Length=249, Percent_Identity=28.5140562248996, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24583079, Length=249, Percent_Identity=28.5140562248996, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI19921000, Length=249, Percent_Identity=28.5140562248996, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI19922422, Length=274, Percent_Identity=24.8175182481752, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27848; Mature: 27717

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPARSAADGDAVVFERHTDVMVVRLNSPSTRNALQPAVKQHLELRIPELLTDPAVRCLVI
CCCCCCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHCCCCEEEEEE
TGSEDSFCAGGDINYMGDRGAPQVRRRMQATHGWAKMLLTAEKPVIAAVNGSAAGAGFSL
ECCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHH
ALLCDIVLMSDQAYFRAAFPGLGAAPDLGLALTLPRAVGAMRAKDILLTNRRVEAEEALA
HHHHHHHHHCCCHHHHCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCHHHHHH
LGIAKRVVPADTLMAEALQLAADLAAGPATSLGLTKMLVNGAFEPIEQFFALEAFAQGIA
HHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
FGSAEFAEGVAAFQEKRKADFRR
CCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PARSAADGDAVVFERHTDVMVVRLNSPSTRNALQPAVKQHLELRIPELLTDPAVRCLVI
CCCCCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHCCCCEEEEEE
TGSEDSFCAGGDINYMGDRGAPQVRRRMQATHGWAKMLLTAEKPVIAAVNGSAAGAGFSL
ECCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHH
ALLCDIVLMSDQAYFRAAFPGLGAAPDLGLALTLPRAVGAMRAKDILLTNRRVEAEEALA
HHHHHHHHHCCCHHHHCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCHHHHHH
LGIAKRVVPADTLMAEALQLAADLAAGPATSLGLTKMLVNGAFEPIEQFFALEAFAQGIA
HHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
FGSAEFAEGVAAFQEKRKADFRR
CCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA