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The map label for this gene is cobS [H]

Identifier: 192289860

GI number: 192289860

Start: 1532553

End: 1533350

Strand: Reverse

Name: cobS [H]

Synonym: Rpal_1450

Alternate gene names: 192289860

Gene position: 1533350-1532553 (Counterclockwise)

Preceding gene: 192289865

Following gene: 192289852

Centisome position: 26.69

GC content: 69.3

Gene sequence:

>798_bases
ATGACCGACTCCCCCCAAGTTTCGCGGCCGACGCCGACGCTGGCGCAATTGCTCGACGCCGTACGTTTCCTGACCATTCT
GCCGGTGCCGAGCGCCGACCGTGCGCCGGAGCCGGACTGGCTGATGCGGGCGATGACGTTTTTTCCGGTGGTCGGAGTTG
GCATCGGCGTGGTGTCGGCGGCGGCGATGATCGTCGGCACCTCGCTGTTCGGGCCAATGCTCGGCGCCCTACTAGCCGTG
ACCGCCTCGATCGCGCTGACGGGCGCGTTCCACGAAGACGGACTCGCCGACACCTTCGATGCGTTCGGCGGCGGCTGGAC
GGTCGAGCGCCGGCTCGAGATCATGAAGGACAGCCGGATCGGCACCTATGGCGCGCTCGCACTCGGCGTCGGCGTCGCGC
TGCGAGTTGCGGCGCTGTCGCTATTGCCGCTGTGGGCGGCACTGGCCGCGCTGGTCGCCTGCCACGCGGCGTCGCGCGGC
GCCTCGTGTCTGGTCGCACACCGGATGAATTACGCCGGCAACACCGCGGCGATGAAGGCGAGCTACGCCGACACTACGCT
GCGCAAGGATGAACTTGTGCTGGGCGGAATCGCGGTCGCGCTTGGCGCGCTGCCGCTGCTCCTGGTGTCGCCGATCGCGG
TGGTGATCGGACTTGCGGCAGGCAGCGTCCTTGCCGCCGCAATGGCCGGCTGGGCCAAGCGGCTGATCGGCGGCTACACC
GGCGACGTGCTCGGCGCCAGCGAGCAACTGTTCGAGATCGGCTTTCTGCTCGGCGTCGCGGCGGTGGTCGCCGGATAG

Upstream 100 bases:

>100_bases
GGTACTAAGGCACCGCGCGACAGCCTGTCGACTGCGTACGCAAGAGCTTGACCGTAGCGGCCTGGGCGCGCAGTACGGCG
GCTTCCGCAAAGGCAGATTC

Downstream 100 bases:

>100_bases
GTCGGTCGCTCAAATTTCTGCGCTATTGCGAGGAGCAAAGCGACGAAGAAATCCAGAATTCGGGGCATCCGTCTCCTGGA
TTGCTTCGCTTCGCTCGCAA

Product: cobalamin 5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MTDSPQVSRPTPTLAQLLDAVRFLTILPVPSADRAPEPDWLMRAMTFFPVVGVGIGVVSAAAMIVGTSLFGPMLGALLAV
TASIALTGAFHEDGLADTFDAFGGGWTVERRLEIMKDSRIGTYGALALGVGVALRVAALSLLPLWAALAALVACHAASRG
ASCLVAHRMNYAGNTAAMKASYADTTLRKDELVLGGIAVALGALPLLLVSPIAVVIGLAAGSVLAAAMAGWAKRLIGGYT
GDVLGASEQLFEIGFLLGVAAVVAG

Sequences:

>Translated_265_residues
MTDSPQVSRPTPTLAQLLDAVRFLTILPVPSADRAPEPDWLMRAMTFFPVVGVGIGVVSAAAMIVGTSLFGPMLGALLAV
TASIALTGAFHEDGLADTFDAFGGGWTVERRLEIMKDSRIGTYGALALGVGVALRVAALSLLPLWAALAALVACHAASRG
ASCLVAHRMNYAGNTAAMKASYADTTLRKDELVLGGIAVALGALPLLLVSPIAVVIGLAAGSVLAAAMAGWAKRLIGGYT
GDVLGASEQLFEIGFLLGVAAVVAG
>Mature_264_residues
TDSPQVSRPTPTLAQLLDAVRFLTILPVPSADRAPEPDWLMRAMTFFPVVGVGIGVVSAAAMIVGTSLFGPMLGALLAVT
ASIALTGAFHEDGLADTFDAFGGGWTVERRLEIMKDSRIGTYGALALGVGVALRVAALSLLPLWAALAALVACHAASRGA
SCLVAHRMNYAGNTAAMKASYADTTLRKDELVLGGIAVALGALPLLLVSPIAVVIGLAAGSVLAAAMAGWAKRLIGGYTG
DVLGASEQLFEIGFLLGVAAVVAG

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=238, Percent_Identity=35.2941176470588, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 26926; Mature: 26795

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDSPQVSRPTPTLAQLLDAVRFLTILPVPSADRAPEPDWLMRAMTFFPVVGVGIGVVSA
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AAMIVGTSLFGPMLGALLAVTASIALTGAFHEDGLADTFDAFGGGWTVERRLEIMKDSRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHHCCCC
GTYGALALGVGVALRVAALSLLPLWAALAALVACHAASRGASCLVAHRMNYAGNTAAMKA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHH
SYADTTLRKDELVLGGIAVALGALPLLLVSPIAVVIGLAAGSVLAAAMAGWAKRLIGGYT
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GDVLGASEQLFEIGFLLGVAAVVAG
CCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA