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The map label for this gene is 192289811

Identifier: 192289811

GI number: 192289811

Start: 1476020

End: 1477423

Strand: Reverse

Name: 192289811

Synonym: Rpal_1401

Alternate gene names: NA

Gene position: 1477423-1476020 (Counterclockwise)

Preceding gene: 192289812

Following gene: 192289809

Centisome position: 25.72

GC content: 66.31

Gene sequence:

>1404_bases
ATGCTTCAGGTGACCGGAATTCCGGATGCGTGCGACAATCTCGCGCCGATCCCAATGGAATGCGACGCGCCGTTCCTCAG
CATCAAGGGCGAGCTGCCGCGGGAATTGAACGGCACGTTGTATCGCAACGGCGCCAACCCGCAATTCGTCTCGCCGAACG
CGCACTGGTTCTTTGGCGACGGCATGCTGCACGCGTTTCATCTGGAGAACGGCCGCGCGTCGTATCGTAACCGCTGGGTG
CGCACGCCGAAATGGCTCGCGGAGCACGAGGCGGGCCGCCCGCTCTACGGCGAGTTCAACCTCAAGCTGCCCGATGCACC
GCGCTCGGTGCCGGACGACGGCAACGTCGCCAACACCAACATCGTGTTCCACGCCGGCCGGCTCCTGGCGCTGGAAGAGG
CGCATCTGCCGATGCAGATCGAACGCGACACGCTCGAGACCCGCGGTTACTGCGACTACGGCGGCGCGCTGAAGGGGCCA
TTCACCGCTCACCCAAAGATCGACCCGGTGACCGGCGAGATGCTGTTCTTCGGCTACAACGCCAGCGGCCCGCTGACGCG
TACGATGTCGTTCGGGGCGATCGACGCCTCGGGCAACGTCACCCGGCTGGAACATTTCAAGGCGCCGTTTGCGGCGATGG
TGCACGACTTCATCGTCACCGAGCATCATGTGCTGTTCCCGATCCTGCCGCTCACCGGCAGTATCTGGCGCGCGATGCGC
GGCCGGCCGCCCTACGCCTGGGATCCGCGCAAGGGCTCGTATGTCGGCGTGATGAAGCGCTCCGGCTCGACCCGCGACAT
CCGCTGGTTCCGCGGCGAGGCCTGCTTCGTGTTCCACGTCATGAACGCGTGGGAGGACGGCACCCGGATCGTCGCCGACG
TGATGCAGTCGGAGGAAGCGCCGCTGTTCACCCATCCCGATGGTCGCCGCACCGATCCGGAGAAGGGCCGTGCGCGACTG
TGCCGCTGGAGCTTCGACCTCGCCGGCAACACCAACGCCTTCAAGCGCAGCTATCTGGACGAGATCAGCGGCGAATTTCC
ACGGATCGACGAGCGCCGTGCCGGCCTGCGCAGCGGCCACGGCTGGTACGCGTGCGCCAGCCCGGAAACACCGATGCTCG
GCATGCTCACTGGACTCGTGCATGTCGACGGCAACGGCCATCGCCGCACTCGCTATCTGCTGCCGACCGGCGATACCATC
GGCGAGCCGGTGTTCGTGCCGCGTGCGGCCGACGCAAACGAAGCCGAAGGCTGGCTGCTCGCGGTGGTGTGGCGCGGCTG
CGAGAATCGCAGCGACCTTGCGGTGTTCAATGCGACCGACATTGCGGCAGGCCCGATCGCCCTGGTGCATCTCGGCCACC
GCATTCCCGACGGCTTCCACGGCAATTGGGTGCCGGCAGGATAA

Upstream 100 bases:

>100_bases
TGCCACAAGAACACGCTCACGCGGTGCAGCCGTTCGGGCGCCTGCTCGCGCTCGATTGACGAGGTCCGAAGCACGACCAA
GAGAGGCTCAGGAGCACGCC

Downstream 100 bases:

>100_bases
CGGATCGTCACCCGCCACGCAGCACAACGCATCGCGCAGCTCATCGGCCGCGATCTTTGTCCTTGAGCAGGCGGCGATAG
TACGTACTTCGGTCGATCCC

Product: carotenoid oxygenase

Products: NA

Alternate protein names: ACO; 8'-apo-beta-carotenal 15,15'-oxygenase; Diox1 [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MLQVTGIPDACDNLAPIPMECDAPFLSIKGELPRELNGTLYRNGANPQFVSPNAHWFFGDGMLHAFHLENGRASYRNRWV
RTPKWLAEHEAGRPLYGEFNLKLPDAPRSVPDDGNVANTNIVFHAGRLLALEEAHLPMQIERDTLETRGYCDYGGALKGP
FTAHPKIDPVTGEMLFFGYNASGPLTRTMSFGAIDASGNVTRLEHFKAPFAAMVHDFIVTEHHVLFPILPLTGSIWRAMR
GRPPYAWDPRKGSYVGVMKRSGSTRDIRWFRGEACFVFHVMNAWEDGTRIVADVMQSEEAPLFTHPDGRRTDPEKGRARL
CRWSFDLAGNTNAFKRSYLDEISGEFPRIDERRAGLRSGHGWYACASPETPMLGMLTGLVHVDGNGHRRTRYLLPTGDTI
GEPVFVPRAADANEAEGWLLAVVWRGCENRSDLAVFNATDIAAGPIALVHLGHRIPDGFHGNWVPAG

Sequences:

>Translated_467_residues
MLQVTGIPDACDNLAPIPMECDAPFLSIKGELPRELNGTLYRNGANPQFVSPNAHWFFGDGMLHAFHLENGRASYRNRWV
RTPKWLAEHEAGRPLYGEFNLKLPDAPRSVPDDGNVANTNIVFHAGRLLALEEAHLPMQIERDTLETRGYCDYGGALKGP
FTAHPKIDPVTGEMLFFGYNASGPLTRTMSFGAIDASGNVTRLEHFKAPFAAMVHDFIVTEHHVLFPILPLTGSIWRAMR
GRPPYAWDPRKGSYVGVMKRSGSTRDIRWFRGEACFVFHVMNAWEDGTRIVADVMQSEEAPLFTHPDGRRTDPEKGRARL
CRWSFDLAGNTNAFKRSYLDEISGEFPRIDERRAGLRSGHGWYACASPETPMLGMLTGLVHVDGNGHRRTRYLLPTGDTI
GEPVFVPRAADANEAEGWLLAVVWRGCENRSDLAVFNATDIAAGPIALVHLGHRIPDGFHGNWVPAG
>Mature_467_residues
MLQVTGIPDACDNLAPIPMECDAPFLSIKGELPRELNGTLYRNGANPQFVSPNAHWFFGDGMLHAFHLENGRASYRNRWV
RTPKWLAEHEAGRPLYGEFNLKLPDAPRSVPDDGNVANTNIVFHAGRLLALEEAHLPMQIERDTLETRGYCDYGGALKGP
FTAHPKIDPVTGEMLFFGYNASGPLTRTMSFGAIDASGNVTRLEHFKAPFAAMVHDFIVTEHHVLFPILPLTGSIWRAMR
GRPPYAWDPRKGSYVGVMKRSGSTRDIRWFRGEACFVFHVMNAWEDGTRIVADVMQSEEAPLFTHPDGRRTDPEKGRARL
CRWSFDLAGNTNAFKRSYLDEISGEFPRIDERRAGLRSGHGWYACASPETPMLGMLTGLVHVDGNGHRRTRYLLPTGDTI
GEPVFVPRAADANEAEGWLLAVVWRGCENRSDLAVFNATDIAAGPIALVHLGHRIPDGFHGNWVPAG

Specific function: Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta- carotene or lyco

COG id: COG3670

COG function: function code Q; Lignostilbene-alpha,beta-dioxygenase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid oxygenase family [H]

Homologues:

Organism=Homo sapiens, GI82617628, Length=542, Percent_Identity=22.3247232472325, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI82617624, Length=542, Percent_Identity=22.6937269372694, Blast_Score=80, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004294 [H]

Pfam domain/function: PF03055 RPE65 [H]

EC number: =1.14.99.41 [H]

Molecular weight: Translated: 51936; Mature: 51936

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQVTGIPDACDNLAPIPMECDAPFLSIKGELPRELNGTLYRNGANPQFVSPNAHWFFGD
CEEECCCCHHHCCCCCCCCCCCCCEEEECCCCCCCCCCEEEECCCCCCEECCCCCEEECC
GMLHAFHLENGRASYRNRWVRTPKWLAEHEAGRPLYGEFNLKLPDAPRSVPDDGNVANTN
CEEEEEEECCCCHHHHHCCCCCCHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCEECCE
IVFHAGRLLALEEAHLPMQIERDTLETRGYCDYGGALKGPFTAHPKIDPVTGEMLFFGYN
EEEECCEEEEEECCCCCEEEECCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEC
ASGPLTRTMSFGAIDASGNVTRLEHFKAPFAAMVHDFIVTEHHVLFPILPLTGSIWRAMR
CCCCEEEEEEEEEEECCCCEEEHHHHCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHC
GRPPYAWDPRKGSYVGVMKRSGSTRDIRWFRGEACFVFHVMNAWEDGTRIVADVMQSEEA
CCCCCCCCCCCCCEEEEEECCCCCCCEEEECCCEEEEEEEEHHCCCCHHHHHHHHHCCCC
PLFTHPDGRRTDPEKGRARLCRWSFDLAGNTNAFKRSYLDEISGEFPRIDERRAGLRSGH
CEEECCCCCCCCCHHHHHEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCC
GWYACASPETPMLGMLTGLVHVDGNGHRRTRYLLPTGDTIGEPVFVPRAADANEAEGWLL
CEEEECCCCCCHHEEEEEEEEECCCCCEEEEEEECCCCCCCCCEEEECCCCCCCCCCEEE
AVVWRGCENRSDLAVFNATDIAAGPIALVHLGHRIPDGFHGNWVPAG
EEEECCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLQVTGIPDACDNLAPIPMECDAPFLSIKGELPRELNGTLYRNGANPQFVSPNAHWFFGD
CEEECCCCHHHCCCCCCCCCCCCCEEEECCCCCCCCCCEEEECCCCCCEECCCCCEEECC
GMLHAFHLENGRASYRNRWVRTPKWLAEHEAGRPLYGEFNLKLPDAPRSVPDDGNVANTN
CEEEEEEECCCCHHHHHCCCCCCHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCEECCE
IVFHAGRLLALEEAHLPMQIERDTLETRGYCDYGGALKGPFTAHPKIDPVTGEMLFFGYN
EEEECCEEEEEECCCCCEEEECCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEC
ASGPLTRTMSFGAIDASGNVTRLEHFKAPFAAMVHDFIVTEHHVLFPILPLTGSIWRAMR
CCCCEEEEEEEEEEECCCCEEEHHHHCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHC
GRPPYAWDPRKGSYVGVMKRSGSTRDIRWFRGEACFVFHVMNAWEDGTRIVADVMQSEEA
CCCCCCCCCCCCCEEEEEECCCCCCCEEEECCCEEEEEEEEHHCCCCHHHHHHHHHCCCC
PLFTHPDGRRTDPEKGRARLCRWSFDLAGNTNAFKRSYLDEISGEFPRIDERRAGLRSGH
CEEECCCCCCCCCHHHHHEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCC
GWYACASPETPMLGMLTGLVHVDGNGHRRTRYLLPTGDTIGEPVFVPRAADANEAEGWLL
CEEEECCCCCCHHEEEEEEEEECCCCCEEEEEEECCCCCCCCCEEEECCCCCCCCCCEEE
AVVWRGCENRSDLAVFNATDIAAGPIALVHLGHRIPDGFHGNWVPAG
EEEECCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]