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The map label for this gene is pldB [H]

Identifier: 192289384

GI number: 192289384

Start: 1017115

End: 1018059

Strand: Reverse

Name: pldB [H]

Synonym: Rpal_0956

Alternate gene names: 192289384

Gene position: 1018059-1017115 (Counterclockwise)

Preceding gene: 192289386

Following gene: 192289383

Centisome position: 17.72

GC content: 66.67

Gene sequence:

>945_bases
ATGACGCTCGTCTCGACTCCTGCCAATCCGGTGCCCGAAGACGCCGTCTCCGGCTTCATCAAGACGCCCGATGGCGTCCA
GCTGCGGTTTGCGCGATGGGAGCCGCCGGGGCACCGCAAAGGCACCGTATGCGTCTTCACCGGACGCACCGAACAGATCG
AGAAGTACTTCGAGACGGTGCGCGATCTGCGCGATCGCGGCTTTGCAGTCGCGGCGCTCGACTGGCGCGGCCAGGGCCAT
TCCGAGCGCCGGCTGCGCGACTATCGCAAGGGCTATGTGCGCAACTTCGCCGACTTCGAGGTCGACGTCGAAACCTTCGT
GCGTGAAGTCGTGCTGCCGGACTGCCCGCCGCCGCATTTCGCGCTGGCGCATTCGATGGGCGGGGCGGTGATGCTGCGGA
TCGCCCGCTCCGGCAAGCGCTGGTTCGACCGCATGGTGCTGTCGGCCCCGATGATCGACCTTGCCGGCAACACCACCGCG
CTGCCGATCAGGCTGTTGCTGCGGGCGATGCGGCTGGCCGGCCAAGGCGGCCGTTACGTTCCCGGCGGCAACGACGTGCT
CGCCAACACCGAGCCGTTCGTCGGAAACAAGCTGACCTCGGATCCGGTGCGCTACGCCCGCAACGCCGCGATCGTCGCCG
AAGATCCGACCCTGGGCCTCGCCTCGCCGACCGTCGCATGGGCCGACACCGCTTTCCGCGCCATGCACGCCTTCAAGGCG
ACCAACTATCCGTCGCAGATCCGGCAGCCGATCCTGATGATCGCGGCCGGTATGGACGGCGTGGTGTCGACGCCGGCGAT
CGAAGAGTTCGCTTATCATTTGCGGGCCGGCTCGCATCTGGTGATCCCCGGCGCCAAGCACGAAATCCTCCAGGAGCAGG
ATCGCTACCGCCAGCAATTCTGGGCGGCGTTCGATGCTTTCGTACCGGGGACGCCGCTTTTCTGA

Upstream 100 bases:

>100_bases
GGGAGGGGTGTTTCGGGGTTCAAGAGGGGGCCCGAAGCTGTTCGCGCCCGCCGCTTTCATCCAGATTTTCCAGGCATTTT
CTCCCCTCCGGACTCGGTCC

Downstream 100 bases:

>100_bases
TGTGACATTCTGTCTCCGGCGACGCTTCGCGCCGCGTTTCGGAACTTCACACATCCGCCTTGGTTGCAGGCTGCAAGACC
GTCCGGTTTCGCCTTTCCGT

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: Lecithinase B [H]

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MTLVSTPANPVPEDAVSGFIKTPDGVQLRFARWEPPGHRKGTVCVFTGRTEQIEKYFETVRDLRDRGFAVAALDWRGQGH
SERRLRDYRKGYVRNFADFEVDVETFVREVVLPDCPPPHFALAHSMGGAVMLRIARSGKRWFDRMVLSAPMIDLAGNTTA
LPIRLLLRAMRLAGQGGRYVPGGNDVLANTEPFVGNKLTSDPVRYARNAAIVAEDPTLGLASPTVAWADTAFRAMHAFKA
TNYPSQIRQPILMIAAGMDGVVSTPAIEEFAYHLRAGSHLVIPGAKHEILQEQDRYRQQFWAAFDAFVPGTPLF

Sequences:

>Translated_314_residues
MTLVSTPANPVPEDAVSGFIKTPDGVQLRFARWEPPGHRKGTVCVFTGRTEQIEKYFETVRDLRDRGFAVAALDWRGQGH
SERRLRDYRKGYVRNFADFEVDVETFVREVVLPDCPPPHFALAHSMGGAVMLRIARSGKRWFDRMVLSAPMIDLAGNTTA
LPIRLLLRAMRLAGQGGRYVPGGNDVLANTEPFVGNKLTSDPVRYARNAAIVAEDPTLGLASPTVAWADTAFRAMHAFKA
TNYPSQIRQPILMIAAGMDGVVSTPAIEEFAYHLRAGSHLVIPGAKHEILQEQDRYRQQFWAAFDAFVPGTPLF
>Mature_313_residues
TLVSTPANPVPEDAVSGFIKTPDGVQLRFARWEPPGHRKGTVCVFTGRTEQIEKYFETVRDLRDRGFAVAALDWRGQGHS
ERRLRDYRKGYVRNFADFEVDVETFVREVVLPDCPPPHFALAHSMGGAVMLRIARSGKRWFDRMVLSAPMIDLAGNTTAL
PIRLLLRAMRLAGQGGRYVPGGNDVLANTEPFVGNKLTSDPVRYARNAAIVAEDPTLGLASPTVAWADTAFRAMHAFKAT
NYPSQIRQPILMIAAGMDGVVSTPAIEEFAYHLRAGSHLVIPGAKHEILQEQDRYRQQFWAAFDAFVPGTPLF

Specific function: Unknown

COG id: COG2267

COG function: function code I; Lysophospholipase

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI48994980, Length=283, Percent_Identity=31.8021201413428, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 34864; Mature: 34733

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLVSTPANPVPEDAVSGFIKTPDGVQLRFARWEPPGHRKGTVCVFTGRTEQIEKYFETV
CCEECCCCCCCCHHHHCCCEECCCCCEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHH
RDLRDRGFAVAALDWRGQGHSERRLRDYRKGYVRNFADFEVDVETFVREVVLPDCPPPHF
HHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHCCEECHHHHHHHHHCCCCCCCHH
ALAHSMGGAVMLRIARSGKRWFDRMVLSAPMIDLAGNTTALPIRLLLRAMRLAGQGGRYV
HHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCC
PGGNDVLANTEPFVGNKLTSDPVRYARNAAIVAEDPTLGLASPTVAWADTAFRAMHAFKA
CCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHC
TNYPSQIRQPILMIAAGMDGVVSTPAIEEFAYHLRAGSHLVIPGAKHEILQEQDRYRQQF
CCCHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHH
WAAFDAFVPGTPLF
HHHHHHHCCCCCCC
>Mature Secondary Structure 
TLVSTPANPVPEDAVSGFIKTPDGVQLRFARWEPPGHRKGTVCVFTGRTEQIEKYFETV
CEECCCCCCCCHHHHCCCEECCCCCEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHH
RDLRDRGFAVAALDWRGQGHSERRLRDYRKGYVRNFADFEVDVETFVREVVLPDCPPPHF
HHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHCCEECHHHHHHHHHCCCCCCCHH
ALAHSMGGAVMLRIARSGKRWFDRMVLSAPMIDLAGNTTALPIRLLLRAMRLAGQGGRYV
HHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCC
PGGNDVLANTEPFVGNKLTSDPVRYARNAAIVAEDPTLGLASPTVAWADTAFRAMHAFKA
CCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHC
TNYPSQIRQPILMIAAGMDGVVSTPAIEEFAYHLRAGSHLVIPGAKHEILQEQDRYRQQF
CCCHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHH
WAAFDAFVPGTPLF
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]