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The map label for this gene is lig [H]

Identifier: 192289298

GI number: 192289298

Start: 922080

End: 923948

Strand: Reverse

Name: lig [H]

Synonym: Rpal_0870

Alternate gene names: 192289298

Gene position: 923948-922080 (Counterclockwise)

Preceding gene: 192289299

Following gene: 192289297

Centisome position: 16.09

GC content: 67.42

Gene sequence:

>1869_bases
ATGAATCGCTTCGCCGAACTGCTCGACCGCCTCGGCTATGAGCCCGGCCGTAACAACAAGCTGCGGCTGATCACCAATTA
CTTTCGCGAGGTCGAAGATCCCGACCGCGGCTGGGCGCTTGCAGCGTTGACCGGCGCGCTGTCGTTCCGCCATGCCAAGC
CCGGCCTGATCCGCGCCCTGATCGCCGAACGCACCGACCCGGTGCTGTTCGGCCTGTCCTACGACTACGTCGGCGATTTG
TCGGAGACGGTGGCGCTGATGTGGCCCTCTCCCCGCAACGCGGCCGTCTCCTCTCCCCCAGGCTGGGCCCCCTCCCCTCC
CCCCTGCGGGGAGGGGTCGGGGGTGGGGGTCCACACCGGGACGTCATCCTCGGGGCGCCCTCACCCCACCCCTCTCCCGC
AAGCGGGAGAGGGAGCGCGCCGCGCATCGGGAGAGGACGGCGATTCCGAACTAAGCTCCGCAACGAGCACCGGCCACAAC
AACCCGCCGCCCCCGACACTCAGCGAGATCGTCACTACGCTGCACAGTCTCGGCAAGGCGGAGCTGCCGGCGCAGTTGGC
GCGCTGGCTCGATGAGCTCGACGAGACCGGGCGCTGGGCGCTGCTCAAGCTGGTGACCGGGGGCTTGCGGATCGGCGTGT
CGGCGCGGCTCGCGAAGACCGCCACTGCGGCGCTCGGCGACAAGGATCCCCACGACGTCGAACTGGTGTGGCCCGGACTC
CAGCCGCCATATCGCGAGCTGTTCGCCTGGCTCGAAGACCGCGGGCCGCAGCCGGTCAATCGCGATCCGGCGCCGTTCCG
CCCGGTGATGCTGGCGCATGCGATCGACGACACCGACTTCGCCGCGATGAATCCGGCCGACTACATCGCCGAATGGAAAT
GGGACGGCATCCGGGTCCAGGCGGTCAGCGGCCAGAACGAGCACGGGGACACGGTCGTGCGGCTGTATTCGCGGAGCGGC
GAAGACATCACCATGAGCTTCCCCGATCTGTTGCCGTCGCTGCATCTTCCCGGCGCCATCGATGGCGAGCTCCTGGTGCT
GCGTGACAAGCGCGTGCAATCGTTCAACGTGCTGCAGCAGCGGCTGAACCGTAAATCAGTCACGCCGAAGTTGACGAAGG
ACTATCCGATCCATCTGCGCGCCTATGACCTGCTCGGCGAAGGCGACGAGGACCTGCGAACACTGCCGTTCGCCGAGCGA
CGCGCCCGCCTCGAGACCTTCATCGCGAGGCTCGACGATCCCAAGGTCGACCTGTCGCCGATCGTCACCTTCGACGATTG
GGAAGCCTTGCGCGCGGCACGCCGCGATCCGGCGAGTACCGGCGCCGGGCTCGATGCCGACGCGGTCGAAGGCGTGATGC
TGAAGCGGCGCGACGCGCTGTATCTGCCTGGCCGCCCCAAGGGCCAGTGGTGGAAGTGGAAGCACGACCCGCACATCATC
GATGCCGTGCTGATGTATGCGCAGCGCGGCCACGGCAAACGCTCGTCGTATTATTCCGACTACACCTTCGGGGTCTGGAC
CGAAGGCGACGGCGGCGATCAGCTCGTGCCGGTCGGCAAGGCGTATTTCGGCTTCACCGACGAAGAGCTGCTGCAGATCG
ACCGCTTCGTCCGCCGCAACACCACCGAGAAATTCGGCCCCGTCCGGCATGTCGTGCACGAGCCCGACAAGGGCCTAGTG
CTGGAAGTGGCGTTCGAAGGATTGCAGCGCTCGCCGCGCCACAAATCCGGCGTAGCGATGCGGTTTCCCCGCATCAACCG
GCTGCGCTGGGACAAGCCGCCGAAGGACGCCGACCGGCTGGACACGCTGGAGCGGATGCTGAAGGCGGAGACCGCAAATC
CGCCGGCGACCGCCGCCCGCGGCCATTGA

Upstream 100 bases:

>100_bases
AGACCAAGGGCCTCGCTGCGCGGCCGCTCGACCTGGTCGGCTATGGCGACGAAGATGAAGACGTCGGCATGCCGACCGGC
GAGAGCGAGGCCGCAGAGGC

Downstream 100 bases:

>100_bases
CGGCAAAGCGCCGGTTCCCCATCTGCCCAGCACCGAATATGATGCGCCAGCCACGCGCGAGGACCATGACAATGCGAGAC
GACATCCGGGATCTGCCGGC

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 622; Mature: 622

Protein sequence:

>622_residues
MNRFAELLDRLGYEPGRNNKLRLITNYFREVEDPDRGWALAALTGALSFRHAKPGLIRALIAERTDPVLFGLSYDYVGDL
SETVALMWPSPRNAAVSSPPGWAPSPPPCGEGSGVGVHTGTSSSGRPHPTPLPQAGEGARRASGEDGDSELSSATSTGHN
NPPPPTLSEIVTTLHSLGKAELPAQLARWLDELDETGRWALLKLVTGGLRIGVSARLAKTATAALGDKDPHDVELVWPGL
QPPYRELFAWLEDRGPQPVNRDPAPFRPVMLAHAIDDTDFAAMNPADYIAEWKWDGIRVQAVSGQNEHGDTVVRLYSRSG
EDITMSFPDLLPSLHLPGAIDGELLVLRDKRVQSFNVLQQRLNRKSVTPKLTKDYPIHLRAYDLLGEGDEDLRTLPFAER
RARLETFIARLDDPKVDLSPIVTFDDWEALRAARRDPASTGAGLDADAVEGVMLKRRDALYLPGRPKGQWWKWKHDPHII
DAVLMYAQRGHGKRSSYYSDYTFGVWTEGDGGDQLVPVGKAYFGFTDEELLQIDRFVRRNTTEKFGPVRHVVHEPDKGLV
LEVAFEGLQRSPRHKSGVAMRFPRINRLRWDKPPKDADRLDTLERMLKAETANPPATAARGH

Sequences:

>Translated_622_residues
MNRFAELLDRLGYEPGRNNKLRLITNYFREVEDPDRGWALAALTGALSFRHAKPGLIRALIAERTDPVLFGLSYDYVGDL
SETVALMWPSPRNAAVSSPPGWAPSPPPCGEGSGVGVHTGTSSSGRPHPTPLPQAGEGARRASGEDGDSELSSATSTGHN
NPPPPTLSEIVTTLHSLGKAELPAQLARWLDELDETGRWALLKLVTGGLRIGVSARLAKTATAALGDKDPHDVELVWPGL
QPPYRELFAWLEDRGPQPVNRDPAPFRPVMLAHAIDDTDFAAMNPADYIAEWKWDGIRVQAVSGQNEHGDTVVRLYSRSG
EDITMSFPDLLPSLHLPGAIDGELLVLRDKRVQSFNVLQQRLNRKSVTPKLTKDYPIHLRAYDLLGEGDEDLRTLPFAER
RARLETFIARLDDPKVDLSPIVTFDDWEALRAARRDPASTGAGLDADAVEGVMLKRRDALYLPGRPKGQWWKWKHDPHII
DAVLMYAQRGHGKRSSYYSDYTFGVWTEGDGGDQLVPVGKAYFGFTDEELLQIDRFVRRNTTEKFGPVRHVVHEPDKGLV
LEVAFEGLQRSPRHKSGVAMRFPRINRLRWDKPPKDADRLDTLERMLKAETANPPATAARGH
>Mature_622_residues
MNRFAELLDRLGYEPGRNNKLRLITNYFREVEDPDRGWALAALTGALSFRHAKPGLIRALIAERTDPVLFGLSYDYVGDL
SETVALMWPSPRNAAVSSPPGWAPSPPPCGEGSGVGVHTGTSSSGRPHPTPLPQAGEGARRASGEDGDSELSSATSTGHN
NPPPPTLSEIVTTLHSLGKAELPAQLARWLDELDETGRWALLKLVTGGLRIGVSARLAKTATAALGDKDPHDVELVWPGL
QPPYRELFAWLEDRGPQPVNRDPAPFRPVMLAHAIDDTDFAAMNPADYIAEWKWDGIRVQAVSGQNEHGDTVVRLYSRSG
EDITMSFPDLLPSLHLPGAIDGELLVLRDKRVQSFNVLQQRLNRKSVTPKLTKDYPIHLRAYDLLGEGDEDLRTLPFAER
RARLETFIARLDDPKVDLSPIVTFDDWEALRAARRDPASTGAGLDADAVEGVMLKRRDALYLPGRPKGQWWKWKHDPHII
DAVLMYAQRGHGKRSSYYSDYTFGVWTEGDGGDQLVPVGKAYFGFTDEELLQIDRFVRRNTTEKFGPVRHVVHEPDKGLV
LEVAFEGLQRSPRHKSGVAMRFPRINRLRWDKPPKDADRLDTLERMLKAETANPPATAARGH

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

Organism=Homo sapiens, GI4557719, Length=576, Percent_Identity=24.6527777777778, Blast_Score=87, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71985269, Length=381, Percent_Identity=27.5590551181102, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320038, Length=358, Percent_Identity=26.2569832402235, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24762424, Length=384, Percent_Identity=25.2604166666667, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 69052; Mature: 69052

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00697 DNA_LIGASE_A1 ; PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFAELLDRLGYEPGRNNKLRLITNYFREVEDPDRGWALAALTGALSFRHAKPGLIRAL
CCHHHHHHHHHCCCCCCCCCEEHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCHHHHHH
IAERTDPVLFGLSYDYVGDLSETVALMWPSPRNAAVSSPPGWAPSPPPCGEGSGVGVHTG
HHCCCCCEEEECCHHHHCCCHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECC
TSSSGRPHPTPLPQAGEGARRASGEDGDSELSSATSTGHNNPPPPTLSEIVTTLHSLGKA
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCC
ELPAQLARWLDELDETGRWALLKLVTGGLRIGVSARLAKTATAALGDKDPHDVELVWPGL
CHHHHHHHHHHHHHCCCCEEEEHHHHCCCEECCHHHHHHHHHHHCCCCCCCCEEEEECCC
QPPYRELFAWLEDRGPQPVNRDPAPFRPVMLAHAIDDTDFAAMNPADYIAEWKWDGIRVQ
CCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHCCCCCEEEE
AVSGQNEHGDTVVRLYSRSGEDITMSFPDLLPSLHLPGAIDGELLVLRDKRVQSFNVLQQ
EECCCCCCCCEEEEEECCCCCEEEEECHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHH
RLNRKSVTPKLTKDYPIHLRAYDLLGEGDEDLRTLPFAERRARLETFIARLDDPKVDLSP
HHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCE
IVTFDDWEALRAARRDPASTGAGLDADAVEGVMLKRRDALYLPGRPKGQWWKWKHDPHII
EEEECCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCHHH
DAVLMYAQRGHGKRSSYYSDYTFGVWTEGDGGDQLVPVGKAYFGFTDEELLQIDRFVRRN
HHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCEEECCHHHCCCCHHHHHHHHHHHHHC
TTEKFGPVRHVVHEPDKGLVLEVAFEGLQRSPRHKSGVAMRFPRINRLRWDKPPKDADRL
CHHHHHHHHHHHCCCCCCEEEHEEHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCHHHHH
DTLERMLKAETANPPATAARGH
HHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNRFAELLDRLGYEPGRNNKLRLITNYFREVEDPDRGWALAALTGALSFRHAKPGLIRAL
CCHHHHHHHHHCCCCCCCCCEEHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCHHHHHH
IAERTDPVLFGLSYDYVGDLSETVALMWPSPRNAAVSSPPGWAPSPPPCGEGSGVGVHTG
HHCCCCCEEEECCHHHHCCCHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECC
TSSSGRPHPTPLPQAGEGARRASGEDGDSELSSATSTGHNNPPPPTLSEIVTTLHSLGKA
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCC
ELPAQLARWLDELDETGRWALLKLVTGGLRIGVSARLAKTATAALGDKDPHDVELVWPGL
CHHHHHHHHHHHHHCCCCEEEEHHHHCCCEECCHHHHHHHHHHHCCCCCCCCEEEEECCC
QPPYRELFAWLEDRGPQPVNRDPAPFRPVMLAHAIDDTDFAAMNPADYIAEWKWDGIRVQ
CCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHCCCCCEEEE
AVSGQNEHGDTVVRLYSRSGEDITMSFPDLLPSLHLPGAIDGELLVLRDKRVQSFNVLQQ
EECCCCCCCCEEEEEECCCCCEEEEECHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHH
RLNRKSVTPKLTKDYPIHLRAYDLLGEGDEDLRTLPFAERRARLETFIARLDDPKVDLSP
HHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCE
IVTFDDWEALRAARRDPASTGAGLDADAVEGVMLKRRDALYLPGRPKGQWWKWKHDPHII
EEEECCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCHHH
DAVLMYAQRGHGKRSSYYSDYTFGVWTEGDGGDQLVPVGKAYFGFTDEELLQIDRFVRRN
HHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCEEECCHHHCCCCHHHHHHHHHHHHHC
TTEKFGPVRHVVHEPDKGLVLEVAFEGLQRSPRHKSGVAMRFPRINRLRWDKPPKDADRL
CHHHHHHHHHHHCCCCCCEEEHEEHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCHHHHH
DTLERMLKAETANPPATAARGH
HHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA